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Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor alpha and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells

100%

He C.-L., Yi Q., Li Y. F., Yang H., Wang L.

Bulletin of the Veterinary Institute in Puławy

2013

tom 57

nr 3

Mammary epithelial cells (MECs) from Kunming mice were isolated and stimulated in vitro with 10 µg/mL of Escherichia coli lipopolysaccharide (LPS). The release of tumour necrosis factor α (TNF-α) and interleukin-8 (IL-8) into culture supernatants was measured by ELISA. Furthermore, blocking experiments with Toll-like receptor 2 (TLR2) and TLR4 antibodies were performed to verify whether cytokine secretion depended on LPS-induced activation of TLR2 or TLR4. The results revealed that LPS-stimulated mouse MECs significantly secreted TNF-α and IL-8. Blocking of the TLR4 pathway inhibited the secretion of TNF-α and IL-8, while inhibition of LPS-induced TNF-α and IL-8 production was not observed when TLR2 was blocked. Thus, TLR4 can mediate the LPS-induced expression of cytokines such as TNF-α and IL-8 in mouse MECs.

Measurement of milk D-3-hydroxybutyrate with a simple UV spectrophotometer method: an alternative assay method

72%

Zhang Z. G., Liu G. W., Li X. B., Li Y. F., Guo C. M., Gao L., Wang H. B., Xu S. W., Wang Z.

Polish Journal of Veterinary Sciences

2009

tom 12

nr 4

The measurement of D-3-hydroxybutyrate (D-BHBA) in milk samples is an important tool for diagnosis of subclinical/clinical ketosis in dairy cows. We describe a simple UV spectrophotometric method for measuring the concentration of D-BHBA in milk of dairy cows. From two herds, 119 milk samples were taken from dairy cows. The standard-curve equation was y = 0.2582x + 0.0269 (R² = 0.9967). The assay was highly specific with a minimum detection limit of 0.01 mmol/L and measuring range of up to 5 mmol/L. The recovery was between 99.35% and 100.22% and repeatability was 99.8%. The comparison between the spectrophotometric method and the fluorometric method revealed a close correlation (r = 0.9939). These results show that the spectrophotometric method can be successfully used as an alternative method to measure D-BHBA content in milk.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Polish Journal of Veterinary Sciences

2 Li Y. F.

1 Gao L.

1 Guo C. M.

1 He C.-L.

1 Li X. B.

1 Liu G. W.

1 Wang H. B.

1 Wang L.

1 Wang Z.

1 Xu S. W.

1 Yang H.

1 Yi Q.

1 Zhang Z. G.

1 2013

1 2009

Wyniki wyszukiwania

Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor alpha and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells

Measurement of milk D-3-hydroxybutyrate with a simple UV spectrophotometer method: an alternative assay method