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1
Content available remote

Kinetics of calcium ion concentration accompanying signal transduction in neutrophils from children with increased susceptibility to infections

100%
Jakubczak B., Wasik M., Popko K., Demkow U.
Journal of Physiology and Pharmacology. Supplement
|
2006
|
tom 57
|
nr 4
131-137
Increased susceptibility to infections can be a consequence of altered function of immune cells including neutrophils. The goal of the study was to evaluate the process of neutrophil activation via Ca2+-mediated signal. The study was performed on isolated peripheral blood neutrophils obtained from 41 children with recurrent infections (21 girls, 20 boys) 3-10 years old with more than five episodes of respiratory tract infection (RI) per year and from a control group of 30 healthy children age and sex matched, free from allergic, immune and hematological disorders. Neutrophils were activated by bacterial peptide fMLP, opsonized zymosan (OZ), and phorbol myristat acetate (PMA). The kinetics of the intracellular Ca2+ concentration i[Ca2+] was assessed by flow cytometry (Coulter Epics XL) with the use of Fluo3 and Fura Red fluorescent dyes. Data were collected in histograms displaying Fluo3 fluorescence vs. time and Fura Red fluorescence vs. time and the mean channels of fluorescence intensity were used for calculations. fMLP and OZ-induced Ca2+ mobilization lasted shorter in the RI group (P<0.05). The peak influx of free Ca2+ and i[Ca2+] in the resting state after stimulation with fMLP were lower in the patients (P<0.05). In the RI group stimulation with OZ was delayed compared with that in the control group (P<0.01). In response to PMA, i[Ca2+] decreased faster. The kinetic slopes of i[Ca2+] in both groups examined differed statistically at all points measured. A decrease in i[Ca2+] after PMA stimulation was greater (P<0.01) and lasted longer in the RI group. We conclude that increased sensitivity to infections in RI children may be related to the disturbance in neutrophil activation that is mediated by changes in i[Ca2+] with the subsequent production of free oxygen radicals. Such a disturbance may be inheritable or secondary to infection and antibiotic therapy.
2

Wykorzystanie techniki PCR-RELP oraz PCR-SSCP do analizy częstości występowania polimorfizmu genu IL-6 u dzieci z otyłością prostą

88%
Popko K., Pyrzak B., Wisniewska A., Kluczynska-Rymkiewicz B., Wasik M.
Diagnostyka Laboratoryjna
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2004
|
tom 40
|
nr 3
3

Ocena możliwości wykrycia zaburzeń czynności granulocytów testem Chemiluminescencji

88%
Jakubczak B., Popko K., Gorska E., Wasilewska R., Wasik M.
Diagnostyka Laboratoryjna
|
2004
|
tom 40
|
nr 3
4

Ocena chemiluninescencji i zmian stężenia jonów wapnia w granulocytach krwi obwodowej

76%
Jakubczak B., Demkow U., Gorska E., Popko K., Potapinska O., Wasik M.
Diagnostyka Laboratoryjna
|
2007
|
tom 43
|
nr 3
5

Hemophagocytic syndrome in children and adults

76%
Malinowska I., Machaczka M., Popko K., Siwicka A., Salamonowicz M., Nasilowska-Adamska B.
Archivum Immunologiae et Therapiae Experimentalis
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2014
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tom 62
|
nr 5
6
Content available remote

The Gln223Arg polymorphism of the leptin receptor gene and peripheral blood-bone marrow leptin level in leukemic children

76%
Wasik M., Gorska E., Popko K., Pawelec K., Matysiak M., Demkow U.
Journal of Physiology and Pharmacology. Supplement
|
2006
|
tom 57
|
nr 4
375-383
Leptin is an adipocyte-derived hormone regulating energy homeostasis and body weight. Leptin also plays a role in hematopoesis, cell cycle regulation, and in oncogenesis. The leptin receptor is a single transmembrane protein belonging to the superfamily of cytokine receptors, structurally related to the hemopoietin receptor family. The aim of the study was to evaluate bone marrow and peripheral blood leptin level and frequency of distribution of leptin receptor gene polymorphism Gln223Arg in children with acute leukemia. The examined group included 92 children with acute leukemia (83 ALL and 9 AML) and 39 non-leukemic control children. Leptin level was measured by ELISA method at the day of leukemia diagnosis. Genomic DNA was isolated with the use of a column method and the genotyping of DNA sequence variation was carried out by the restriction enzyme analysis of PCR – amplified DNA. The samples were then electrophoresed on 2.5% agarose gel. Leptin level in leukemic children was lower than in healthy children. Bone marrow leptin level was significantly lower than that in the blood in leukemic children with ALL-T and AML. An analysis of frequency distribution of the Gln233Arg polymorphism in the leptin receptor gene in leukemic children showed lack of differences between the patients and controls. There was no difference in the genotype frequencies between the leukemic AML and ALL groups either. The results indicate a possible relation between the leptin level and leukemia development in children. The effectory effect of the hormone seems not related to Gln223Arg polymorphism of its receptor.
7
Content available remote

The influence of insulin on calcium ion concentration during transduction of signals into neutrophils

76%
Demkow U., Winklewski P., Potapinska O., Popko K., Lipinska A., Wasik M.
Journal of Physiology and Pharmacology. Supplement
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2008
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tom 59
|
nr 6
219-229
8

Przydatność oceny markerów apoptozy i lekowrażliwości limfoblastów w ostrych białaczkach u dzieci

76%
Modzelewska M., Popko K., Jakubczak B., Gorska E., Stelmaszczyk-Emmel A., Wasilewski R., Wasik M.
Diagnostyka Laboratoryjna
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2004
|
tom 40
|
nr 3
9
Content available remote

Kinetics of calcium ion concentration accompanying transduction of signals into neutrophils from diabetic patients and its modification by insulin

76%
Demkow U., Winklewski P., Potapinska O., Popko K., Lipinska A., Wasik M.
Journal of Physiology and Pharmacology. Supplement
|
2009
|
tom 60
|
nr 5
10

Analiza polimorfizmu genów dla cytokin prozapalnych IL-1, IL-6 i TNF alfa u otyłych dorosłych z zespołem bezdechu sennego ( OBS)

64%
Demkow U., Popko K., Gorska E., Stoklosa A., Plywaczewski R., Sliwinski P., Gorecka D., Wasik M.
Diagnostyka Laboratoryjna
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2007
|
tom 43
|
nr 3
11
Content available remote

Frequency of distribution of leptin receptor gene polymorphism in obstructive sleep apnea patients

64%
Popko K., Gorska E., Wasik M., Stoklosa A., Plywaczewski R., Winiarska M., Gorecka D., Sliwinski P., Demkow U.
Journal of Physiology and Pharmacology. Supplement
|
2007
|
tom 58
|
nr 5
12
Content available remote

Frequency of distribution of inflammatory cytokines IL-1, IL-6 and TNF-alpha gene polymorphism in patients with obstructive sleep apnea

52%
Popko K., Gorska E., Potapinska O., Wasik M., Stoklosa A., Plywaczewski R., Winiarska M., Gorecka D., Sliwinski P., Popko M., Szwed T., Demkow U.
Journal of Physiology and Pharmacology. Supplement
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2008
|
tom 59
|
nr 6
607-614
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