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Cytokine mRNA expression and IFN-gamma production in nematode resistant and susceptible line lambs artificially infected with gastro-intestinal nematodes

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Pernthaner A., Vlassoff A., Douch P. G. C., Maass D. R.

Acta Parasitologica

1997

tom 42

nr 1

The mRNA expression of the cytokines interleukin (IL)-2, IL-4 and interferon (IFN)-γ and the production of IFN-γ was examined in mesenteric lymph node cells (MLNC) and CD4⁺ enriched cells populations from resistant and susceptible line lambs by use of reverse transcriptase-polymerase chain reactions (RT-PCR) and ELISA. MLNC obtained from lambs that were previously infected with a mixed gastro-intestinal nematode population were stimulated with Con A or Trichostrongylus colubriformis specific antigen. Four weeks after infection MLNC from both line lambs expressed high levels of mRNA coding for IL-2, IL-4 and IFN-γ. MLNC from resistant line lambs when stimulated with antigen for one day had higher mRNA expression of IL-2 and IFN-γ and after three days of culture had higher levels of IL-4 mRNA than MLNC from susceptible line lambs. Antigen stimulated MLNC of resistant line lambs had a higher IFN-γ production than those from susceptible line lambs. These results indicate that in resistant line lambs the secondary in vitro responses of cytokine mRNA and IFN-γ production to nematode specific antigen differs from those of susceptible line lambs.

Ograniczanie wyników

1 Acta Parasitologica

1 Douch P. G. C.

1 Maass D. R.

1 Pernthaner A.

1 Vlassoff A.

1 1997

Wyniki wyszukiwania

Cytokine mRNA expression and IFN-gamma production in nematode resistant and susceptible line lambs artificially infected with gastro-intestinal nematodes