As a goal of this paper, the assessment of genetic variability of Złotnicka White (ZW) and Zlotnicka Spotted (ZS), was chosen in order to verify the appropriateness of 11 tetrameric Short Tandem Repeats (STRs) panel for use in genetic resources of pigs. Analyses were carried out in sets of 91 ZW and 250 ZS pigs. Seventy-one alleles in ZW and 85 alleles in ZS were detected at all 11 STRs loci. An average number of alleles at locus (MNA) was 6.455 in ZW and 7.727 in ZS. An average number of effective alleles (MNe) was 3.532 in ZW and 3.431 in ZS. Observed heterozygosity Ho was 0.659 in ZW and 0.637 in ZS. On average, polymorphism information content (PIC) reached 0.639 and 0.619 per locus in ZW and ZS. The probability of identity of two independent samples PI using all 11 STRs loci in ZW amounted to 3.118 x 10⁻¹⁰ and to 5.921 x 10⁻¹⁰ in ZS while the probability of identity related individuals PISibs was 1.331 x 10⁻⁴ and 1.749 x 10⁻⁴ in ZW and ZS. The power of exclusion for loci combinations when both parents are known, when only one of the parent is known and for two putative parents P1, P2 and P3 were in ZW versus ZS 0.99903 v. 0.99887, 0.97998 v. 0.97654 and 0.99999 v. 0.99998, respectively. Identified estimates of stated parameters illustrate suitability of tetrameric STRs for practical application in the management of genetic resources, verification of parentage and traceability in ZW and ZS. Based on the results, we recommend the panel of tetrameric STRs loci as suitable for parentage, traceability and differentiation of subpopulations in genetic pig resources of similar history. Hₑ.
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