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The nature of nanoparticles and their effective application has been given considerable attention by researchers in various fields, mainly agriculture. The present investigation examined the foliar effect of zinc oxide nanoparticles (ZnO-NPs) on plant growth profiling, photosynthetic machinery and associated biochemical changes in tomato (Lycopersicon esculentum) following growth in various concentrations (10, 50, 100 and 200 ppm ZnO-NPs). After 15 days of transplantation, ZnO-NPs sprayed to the foliage of tomato plant for five days (35-39 DAS). Treated plants at days 45 and 60 (pre-flowering stage), registered an increase in growth and biomass over their respective control. Among different concentrations of ZnO-NPs [0 (control), 10, 50, 100 and 200 ppm], 50 ppm proved to be the optimum foliar spray treatment and increase the SPAD chlorophyll (27% and 32%), net photosynthetic rate (31% and 35%), leaf protein content (17% and 22%), catalase (CAT, 55% and 61%), peroxidase (POX, 68% and 75%) and superoxide dismutase (SOD, 50% and 55%) activity. Interestingly, significant increases in lycopene (23%), β-carotene (25%) content followed by a decrease in the content of ascorbic acid (38%) in response to above treatments. Number of fruits and fruit yield in the treated plants were also higher (21% and 28%) as compare to respective controls. These results suggest that ZnO-NPs interact with meristematic cells triggering biochemical pathways conductive to an enhancement of growth attribute. Further studies are needed to investigate the mechanisms and the side effects of ZnO-NPs on tomato plants
The plants of mung bean (Vigna radiata L. Wilczek) were raised from the seeds soaked in water (control), IAA or 4-C-IAA (10⁻⁶, 10⁻⁸ or 10⁻¹⁰ M) for 8 or 12 h. The plants were allowed to grow in a net house and were sampled at 30 and 45 days after sowing (DAS). Both IAA and 4-Cl-IAA significantly affected the growth (length, fresh and dry mass of roots and shoots), the number of nodules, their fresh and dry mass and the activity of nitrogenase. However, the contents of nitrogen and carbohydrate exhibited a decrease in response to both the auxins. 4-Cl-IAA, at a concentration of 10⁻⁸ M, generated the best response. Moreover, 4-Cl-IAA at other two concentrations (10⁻⁶ and 10⁻¹⁰ M) was much more active than any of the IAA concentration used.
'Multiple fuels-multiple choice' is a common notion associated with the energy choice theory. Following the same theoretical framework, this study aims to identify: (i) existing sources of energy (ii) the associated factors that determine a household choice of a particular source of energy. The study is based on primary data collected from 100 randomly selected households in two villages in northwest Pakistan. The study found that rural households, though having access to multiple sources of energy, persistently depend on biomass fuels for domestic energy consumption. Nonetheless, rural households exhibit inter-fuel switching according to changes in their socioeconomic conditions. The study concludes that income is not the only determinant of transition from traditional to more convenient forms of energy, other factors such as access to alternative energy sources and consumer preferences also account for a household energy choice.
To investigate the effect of exogenously applied 28-homobrassinolide (HBL) on drought-stressed plants, photosynthesis and antioxidant systems were examined in Indian mustard (Brassica juncea L.). Seedlings of Indian mustard were subjected to drought stress for 7 days at the 8–14 (DS1)/15–21 (DS2) days’ stage of growth and then returned to normal conditions of growth. These seedlings were sprayed with HBL (0.01 µM) at the 30-day stage and were sampled at 60 days to assess the changes in growth, photosynthesis and antioxidant enzymes. Plants exposed to stress at either of the stages of growth exhibited a significant decrease in growth and photosynthesis. The exposure of plants to stress at an earlier stage (DS1) was more inhibitory than that at a later stage (DS2). However, the follow-up treatment with HBL significantly improved the values of these parameters and also overcame the inhibitory effect of water stress. The activity of antioxidant enzymes [catalase (E.C. 1.11.1.6), peroxidase (E.C. 1.11.1.7) and superoxide dismutase (E.C. 1.15.1.1)] and proline content in leaves exhibited an increase in response to both the treatment factors, where their interaction had an additive effect. It was, therefore, concluded that the elevated antioxidant system, at least in part, was responsible for amelioration of the drought stress.
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