Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 12

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Expression analysis and functional characterization of a cold-responsive gene COR15A from Arabidopsis thaliana

100%
Liu D., Li W., Cheng J., Hou L.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 09
The cold-responsive (COR) genes play an important role in cold acclimation of higher plants. Here, a tight correlation between chloroplast functionality and COR15A expression, and the functional characterization of Arabidopsis COR15A involved in salt/osmotic stress, were revealed. COR15A gene is light inducible and expressed in light-grown seedlings. The expression level of COR15A was reduced when chloroplasts were damaged by norflurazon treatment. By using several albino mutants, seca1, secy1, and tic20, all of which exhibited severe defects in both structure and function of chloroplast, it was shown that the accumulation of COR15A mRNA depends on chloroplast functionality. Real-time RT-PCR and GUS-staining assays demonstrated that COR15A was induced by salt/osmotic stress partially via ABA. Overexpression of COR15A in Arabidopsis resulted in the seedlings displaying hypersensitivity to salt/osmotic stress. All these results suggest that plant acquire the ability to fully express COR15A only after the development of functional chloroplasts, COR15A may be involved in response to salt/ osmotic stress during early stages of plant development.
2

Study on vegetation concrete technology for slope protection and greening engineering

100%
Cheng J., Luo X., Shen Z., Guo X.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 6
3

Characteristics of preferential flow paths and their impact on nitrate nitrogen transport on agricultural land

88%
Cheng J., Zhang H., Zhang Y., Chen Y., Wang B.
Polish Journal of Environmental Studies
|
2014
|
tom 23
|
nr 6
Preferential flow is important for solute transport in soil. This study aims to investigate distribution characteristics of preferential flow paths in agricultural land and to determine the effects of preferential flow on nitrate nitrogen transport. Dye tracer experiments were conducted on two farmland plots in Changping County, Beijing. Two undisturbed soil columns (with preferential flow) and two packed soil columns (without preferential flow) were used to determine the influence of preferential flow on nitrate nitrogen transport. The results showed greater nitrate nitrogen movement with a relatively higher velocity in the undisturbed soil columns, which is on average 2.31 times of that in the packed soil column. The breakthrough time of undisturbed soil columns was 12 h with 43% reduction compared with that of the packed soil columns. The preferential transport of NO-3 in the undisturbed soil columns accounted for 43.83% of the total flux and resulted in a 97.60% accumulative leached mass for NO-3 of the total mass. These results indicated that the preferential flow with a limited total flux ratio could lead to a large proportion of NO-3 transport. Tailing phenomenon was observed and found to be a unique feature in the preferential flow’s breakthrough curve. Tailing might be caused by discrepancies between the preferential flow, matrix flow, and penetration rate during infiltration.
4

Experimental study of herbaceous structure effects on reducing nutrient loss

76%
Gao C., Cheng J., Li F., Zhang H., Yang F., Dai J.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 3
The objective of this study was to evaluate nutrient loss under herbaceous cover in simulated field conditions and quantify the influences of the aerial and underground parts of plants in preventing nutrient loss. The experimental settings were as follows: two herbs, i.e., alfalfa (Medicago sativa L.) and green foxtail (Setariaviridis (L.) Beauv.); four covers for each herb (0, 20-30%, 50-60%, and 80-90%); and two treatments (reserving the entire plant and trimming the aerial part of the plant). Through simulated rainfall, runoff and sediment samples were collected at the end of the soil tank and analysed for nitrogen (N) and phosphorus (P). The results indicated that nutrient loss gradually decreased with the increasing plant coverage, and the nutrient loss on the green foxtail slope was lower than that on the alfalfa slope under the same coverage. Although the effect of the alfalfa canopy on nutrient loss prevention was higher than that of the green foxtail, the capacity of the green foxtail root for conserving nutrients is stronger than that of alfalfa due to a different herbaceous plant leaf area index and root morphology distribution. An exponential function was found to characterize the different plant root length densities and nutrient losses on the slope.
5

Review of antibiotic pollution in the seven watersheds in China

76%
Lu P., Fang Y., Barvor J. B., Kennedy Neth N. L., Fan N., Li Z., Cheng J.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 6
6

Cloning, expression and identification by immunohistochemistry of humanized single-chain variable fragment antibody against hepatitis C virus core protein

76%
Lun Y., Cheng J., Zhong Y., Yu Z., Wang Q., Wang F., Feng J.
Polish Journal of Microbiology
|
2011
|
tom 60
|
nr 1
Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis C virus core protein was panned with core protein immobilized on microtiter plate wells. After five rounds of panning 60 phage clones specific to core protein were obtained and one selected clone was sequenced. It was found that the specifically detected antigen consists of 774bp and is capable of encoding 257 amino acids in the patients but not in healthy persons.
7

Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis

76%
Xu Z., Dai F., Cheng J., Lv M., Cheng J. W., Lin B. W.
Cellular and Molecular Biology Letters
|
2018
|
tom 23
Background: Evidence has shown that endogenous H2S plays an important role in the physiological and pathophysiological processes of many organs. The study aimed to explore whether exogenous H2S has a potential therapeutic effect on a rat ovariectomy-induced model of osteoporosis. Methods: The OVX osteoporosis model was established in female Sprague-Dawley rats by full bilateral ovariectomy. The rats were randomly divided into four groups, with the two experimental groups receiving an intraperitoneal injection of GYY4137 or sodium alendronate. The level of H2S in the plasma was determined and common laboratory indicators to diagnose osteoporosis, such as alkaline phosphatase (ALP) activity and the levels of osteocalcin (OCN), calcitonin, parathyroid hormone and leptin were measured. The bone mineral density (BMD) of the 4th and 5th lumbar vertebrae was measured using dual-energy X-ray absorptiometry. The maximum stress of femoral fracture was obtained through a three-point bending test of the femur. Results: The OVX osteoporosis model was successfully established. GYY4137 was injected to increase the level of H2S in the plasma in one group, designated OVX-GYY during the observation period (p < 0.05). At 12 weeks, the BMD value of the fourth lumbar vertebra in the OVX-GYY group had increased (p < 0.05). The BMD femur value in the OVX-vehicle group had decreased (p < 0.05). Bilateral ovariectomy leads to biochemical disorders related to bone metabolism and hormone levels in rat plasma (all p < 0.05). Ovariectomy also reduced blood calcium, blood phosphate and calcitonin, and increased parathyroid hormone and leptin. The opposite results were obtained for the groups with alendronate sodium or GYY4137 treatment (all p < 0.05). Conclusions: Through the slow release of H2S, GYY4137 did an excellent job of simulating endogenous neuroendocrine gaseous signaling molecules. Exogenous H2S had a regulatory effect on osteoporosis in ovariectomized rats, showing potential value for the treatment of human postmenopausal osteoporosis.
8

Comparative transcriptome reveals circadian and hormonal control of adventitious rooting in mulberry hardwood cuttings

76%
Cao X., Du W., Shang C., Shen Q., Liu L., Cheng J.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 11
9

Temporal distribution characteristic and risk analysis of heavy metals in greenhouse vegetable soils

76%
Cao Q., Liu B., Ren Z., Xiao H., Cheng J., Xue W.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 3
10

The effects of litter layer and soil properties on the soil-atmosphere fluxes of greenhouse gases in karst forest, Southwest China

76%
Cheng J., Lee X., Zhou Z., Wang B., Xing Y., Cheng H., Tang Y.
Polish Journal of Ecology
|
2013
|
tom 61
|
nr 1
Temporal variation is a major source of the uncertainty in estimating the fluxes of the greenhouse gases (GHGs) in terrestrial ecosystems, and the GHG fluxes and its affecting factors in the karst region of southwest China remains weakly understood. Using the static chamber technique and gas chromatography method, the CO₂, CH₄ and N₂O fluxes were carried out between 9 and 11 a.m. at 15 day intervals from June 2008 to May 2009 in a Pinus massoniana forest. Two treatments were chosen for this study: undisturbed (soil with litter layer) and disturbed (surface litter removal). Both treatments were found to be the net source of atmospheric CO₂ and N₂O, but a sink of atmospheric CH₄. The seasonality of soil CO₂ emission coincided with the seasonal climate pattern, with high CO₂ emission rates in the hot-wet season and low rates in the cool-dry season. In contrast, seasonal patterns of CH₄ and N₂O fluxes were not clear, although higher CH₄ uptake rates were often observed in autumn and higher N₂O emission rates were often observed in spring (dry-wet season transition). The litter was active in GHG fluxes, and removal of the litter layer reduced soil CO₂ emission (17%) and increased CH₄ uptake (24%) whereas N₂O fluxes were not affected distinctly in the pine forest, indicating that litter layer had an important effect on C exchanges. In the pine forest, soil CO₂ emissions and CH₄ uptakes correlated significantly with soil temperature (r²= 0.87, P <0.01; r²= 0.34, P <0.05, respectively), but had no significant relationship with soil moisture. And there was a significant correlation between CH₄ flux and NH₄⁺-N (r²= 0.39, P < 0.05) and soil inorganic N (r²= 0.48, P <0.05), but no significant correlation was found between CH₄ flux and NO₃⁻-N. Moreover, we found a significant negative logarithmic correlation between N₂O flux and soil NO₃⁻-N concentration (r²= 0.41, P <0.05), and the relationship between CO₂ emission and soil inorganic N content (r²= 0.35, P < 0.05). These results suggested that soil temperature and mineral N dynamics largely affected the temporal GHG exchanges between forest soil and atmosphere.
11

Genome-wide identification of Ziziphus jujuba TCP transcription factors and their expression in response to infection with jujube witches’ broom phytoplasma

64%
Chen P., Li J., Ye X., Tan B., Zheng X., Cheng J., Wang W., Wang H., Gu L., Feng J.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 06
Phytoplasmas are plant pathogenic bacteria that inhabit the phloem sieve elements in infected plants and lack a cell wall. Infection of the woody perennial Ziziphus jujuba with phytoplasma causes jujube witches’ broom (JWB), a disease that causes heavy crop losses. The TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) are plant-specific regulators of plant morphogenesis, development, and defense after phytoplasma infection. While the TCP gene family has been studied in many plants, there is no report on TCPs in jujube. In this study, 21 ZjTCP TFs were identified and bioinformatically analyzed based on the genome of the Dongzao cultivar. Another 2 ZjTCPs were found in the genome of the Junzao cultivar. Physicochemical properties of the ZjTCP proteins were quite varied, indicating possible versatility of functions. ZjTCP proteins ranged from 172 to 590 amino acids (aa), had isoelectric points (pIs) between 5.53 (ZjTCP1) and 9.81 (ZjTCP11), and molecular weights ranging from 19,279.98 (ZjTCP11) to 61,784.17 kDa (ZjTCP19). Interaction network mapping predicted several hubs, such as ZjTCP6, ZjTCP7, ZjTCP8, ZjTCP15, ZjTCP16, and ZjTCP19, among which ZjTCP6 and ZjTCP16 were predicted to function in plant defense and morphogenesis. Spatiotemporal expression analysis of the ZjTCPs showed that ten of the ZjTCP genes were detected after infection with ‘Candidatus Phytoplasma ziziphi’. ZjTCP6, ZjTCP7, ZjTCP16, and ZjTCP19 were up-regulated after phytoplasma infection. ZjTCP16 showed the most significant increase in transcript levels, after the emergence of disease symptoms. ZjTCP12, ZjTCP15, and ZjTCP18 were down-regulated after phytoplasma infection. We concluded that ZjTCP6 and ZjTCP16 were most likely regulatory factors with roles during the plant response to jujube witches’ broom.
12

Purification and functional reconstitution of intact ral-binding GTPase activating protein, RLIP76, in artificial liposomes

64%
Singhal S. S., Singhal J., Cheng J., Pikula S., Sharma R., Zimniak P., Awasthi Y. C., Awasthi S.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 2
We have recently shown that RLIP76, a ral-bind ing GTPase ac ti vat ing pro tein, me­diates ATP-dependent transport of glutathione-conjugates (GS-E) and doxorubicin (DOX) (S. Awasthi eta/., Biochemistry39l9327l2000).TransportfunctionofRLIP76 was found to be in tact de spite con sid er able proteolytic frag men ta tion in prep a ra tions used for those stud ies, sug gest ing ei ther that the re sid ual in tact RLIP76 was re spon si ble for trans port ac tiv ity, or that the trans port ac tiv ity could be re con sti tuted by frag­ments of RLIP76. If the for mer were true, in tact RLIP76 would have a much higher spe cific ac tiv ity for ATP-hydrolysis than the frag mented pro tein. We have ad dressed this ques tion by com par ing trans port prop er ties of re com bi nant RLIP76 and hu man eryth ro cyte mem brane RLIP76 pu ri fied in buff ers treated with either 100 or 500uM serine pro te ase in hib i tor, PMSF. The pu rity and iden tity of re com bi nant and hu man eryth ro cyte RLIP76 was es tab lished by SDS/PAGE and West ern-blot anal y sis. These studies con firmed the or i gin of the 38 kDa pro tein, pre vi ously re ferred to as DNP-SG ATPase, from RLIP76. Higher PMSF con cen tra tion re sulted in lower yield of the 38 kDa band and higher yield of in tact RLIP76 from both hu man and re com bi nant source. In con trast, the sub strate-stimulated ATPase ac tiv ity in pres ence of DNP-SG, doxorubicin, daunorubicin, or colchicine were un af fected by in creased PMSF; similarly, ATP-dependent trans port of doxorubicin in proteo liposomes re con sti tuted with RLIP76 was un af fected by higher PMSF. These re sults in di cated that lim ited proteolysis by serine pro teas es does not ab ro gate the trans port func tion of RLIP76. Com parison of trans port ki net ics for daunorubicin be tween re com bi nant vs hu man erythrocyte RLIP76 re vealed higher spe cific ac tiv ity of trans port for tis sue pu ri fied RLIP76, in di cat ing that ad di tional fac tors pres ent in tis sue pu ri fied RLIP76 can mod u late its transport activity.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.