Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 91

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 5 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 5 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
The objective of this study was to validate TaqMan real-time PCR procedures for the quantification of genetically-modified maize (events: MON810, Bt11, Bt176, and T25) and soybean (event: GTS 40-3-2) with the capillary LightCycler 2.0 instrument. The quantification was done by the amplification of reference maize genes: hmg (MON810, Bt11), zSSIIb (Bt176), and adh1 (T25), and genetically-modified DNA fragments containing a part of the maize genome and modified genes at 5' end (MON810, Bt11, T25), or unique DNA sequence of genes (cryIAb/IVS9) used for the modification (Bt176). As regards GM soybean event GTS 40-3-2, soya specific lectin gene and GM construct-specific DNA fragment (CaMV35S/ CTP- EPSPS) were amplified in real-time PCR reactions. Real-time PCR performance criteria achieved in this validation study proved that it is possible to use the reaction protocol made for 96-well plates real-time PCR instruments at capillary Roche thermocycler. Result of the performance criteria obtained for real-time PCR validation study for the quantification of genetically-modified maize or soybean were satisfactory, close or ever better than the values of inter-laboratory ring-trials.
Genetically modified crops are widely used to produce feed mixtures. European Community law requires products containing above 0.9% of GMO to be labeled. The purpose of the study was to detect, identify and quantify GMO by analyzing DNA. The qualitative methods used were based on PCR technique; however, quantitative GMO analysis based on Real Time PCR technique and Roche LightCycler 2.0 instruments was also applied. 59 of the 109 examined samples of feed contained GM crops. The presence of GMO was detected in 31 samples of soybeans and Soya meal, 4 samples of maize and 24 samples of feed mixtures. The most commonly used GMO in animal feed in Poland is Soya Roundup Ready, tolerant to Roundup herbicide, and it was present in 57 samples of feed. Genetically modified maize was present in only 2 of the 69 samples of maize corn and compound feeds. Additionally, 2 samples of maize were contaminated by Soya Roundup Ready.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 5 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.