Wyniki wyszukiwania

1

Rola społeczeństwa w ochronie i zarządzaniu krajobrazem na przykładzie Caerlaverock i Langholm and Newcastleton Hills w południowej Szkocji

100%

Jaworek J.

Prace Komisji Krajobrazu Kulturowego PTG

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2008

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nr 10

2

Ghrelin and melatonin in the regulation of pancreatic exocrine secretion and maintaining of integrity

100%

Jaworek J.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 5

83-96

Ghrelin and melatonin are produced in the central nervous system and in the gastrointestinal tissues; ghrelin in the stomach, and melatonin - in the liver and in the intestine. Both ghrelin and melatonin have been reported to protect the gastric mucosa against acute lesions and to influence gastrointestinal motility and secretions, however the physiological significance of these peptides in the gastrointestinal tissues remains unknown. In spite of the presence of ghrelin and melatonin receptors in the pancreatic tissue little is known about the role of these peptides in the pancreas. It is very likely that both ghrelin and melatonin, which are released from the gastrointestinal tract in relation to food ingestion, could be implicated in the postprandial stimulation of pancreatic enzyme secretion though the activation of cholinergic entero-pancreatic reflex and CCK release. Our experimental studies have shown that exogenous melatonin, as well as this produced endogenously from its precursor; L-tryptophan, strongly stimulates pancreatic amylase secretion when given intraperitoneally, or into the gut lumen. Intraduodenal administration of ghrelin also increases pancreatic enzyme secretion. This was accompanied by significant increases of CCK plasma levels. Above pancreatostimulatory effects of luminal administration of melatonin or ghrelin were completely reversed by bilateral vagotomy, capsaicin deactivation of sensory nerves or pretreatment of the rats with CCK1 receptor antagonist; tarazepide. Our previous findings have revealed that melatonin, as well as its precursor; L-tryptophan, effectively protects the pancreas against the damage induced by caerulein overstimulation. The beneficial effects of melatonin and L-tryptophan on the pancreas have been related to the ability of melatonin to scavenge the radical oxygen species (ROS), to activate antioxidative enzymes and to modulate the cytokine production. It has been previously shown that systemic application of ghrelin attenuated acute pancreatitis activating the immune defense mechanisms. Our recent data demonstrate that ghrelin is able to prevent pancreatic inflammatory damage though the activation of central nervous mechanisms leading to the improvement of antioxidative properties of pancreatic tissue. The results of experimental studies indicated that melatonin and ghrelin could take a part in the protection of pancreatic tissue against the damage under physiological conditions.

3

Central and peripheral control of pancreatic integrity by leptin and melatonin

100%

Jaworek J.

Journal of Physiology and Pharmacology. Supplement

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2001

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tom 52

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nr 2

4

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effects of prostaglandin of E, F and I series, leukotriene C4 and platelet activating factor on amylase release from isolated rat pancreatic acini

63%

Jaworek J., Konturek S. J.

Journal of Physiology and Pharmacology

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1993

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tom 44

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nr 4

5

Zmiana powierzchni zbiorowisk o charakterze leśnym w dolinie Karpnickiego Potoku jako przykład zmian lesistości w dolnych partiach Sudetów

51%

Jaworek J., Filipiak M., Napierala-Filipiak A.

Sylwan

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2014

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tom 158

|

nr 09

On the basis of historical and recent cadastral maps, topographic maps, and aerial photographs, we analysed changes in forest area within the cadastral units of Karpniki village, in the Karpnicki Potok (SW Poland). In 1823−2012, forest area increased there from about 41% to over 57% of the total area. The greatest increase has been recorded recently. Moreover, at that time over 16% of land classified officially as farmland was naturally colonized by communities dominated by woody plants. This seminatural process in the analysed case does not pose any serious threat to natural resources, but a complete lack of its control may lead to deterioration of the value of the local landscape and development of low−quality forest stands. The observed changes seem to be typical of other areas located in lower parts of the Sudety Mountains.

6

Brain-gut axis in the modulation of pancreatic enzyme secretion

51%

Jaworek J., Nawrot-Porabka K., Leja-Szpak A., Konturek S. J.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 5

7

Krajobraz doliny Karpnickiego Potoku. Zastosowanie systemow GIS w analizach historycznych krajobrazu

51%

Jaworek J., Goluch P., Michalski A.

Architektura Krajobrazu. Studia i Prezentacje

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2010

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nr 1

55-61

8

Effect of enkephalin and morphine on pancereatic secretion

51%

Tasler J., Konturek S. J., Cieszkowski M., Jaworek J.

Acta Physiologica Polonica

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1979

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tom 30

|

nr 1

9

Rozmieszczenie zasobów drzew z rodzaju wiąz (Ulmus spp.) w lasach Polski w świetle dokumentacji leśnej

51%

Napierala-Filipiak A., Filipiak M., Jaworek J.

Sylwan

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2014

|

tom 158

|

nr 11

Elm resources in Poland were analysed on the basis of latest forestry inventory data (updated in 2012). As compared with the 1970s, a remarkable increase was noticed in the total area of forest stands dominated by elms. However, the contribution of elms to the total forest area in Poland is still small (17 653.67 ha, i.e. 0.24% of the total forest area), while forest stands dominated by them cover only 1011.84 hectares (0.01%). Elm forests are the most frequent at alluvial sites, but majority of elm trees is scattered in lowlands, mostly at the fertile potential sites of oak−hornbeam forest. Elms do not prefer any part of Poland, but their contribution is markedly lower in forests of the Sudety Mountains.

10

Leptin is the modulator of HSP60 gene expression in AR42J cells

51%

Bonior J., Jaworek J., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

|

nr 7

135-143

Leptin, circulating protein involved in the control of body weight and energy expenditure received attention as a modulator of immune response of the organism. Leptin receptors have been detected in the pancreas and experimental studies have shown that leptin protects the pancreas against the damage induced by caerulein overstimulation. Heat shock proteins (HSP) are endogenous proteins produced by various cells exposed to high temperature or to the noxious agents. HSP protect the cells against various environmental and endogenous stressors. The implication of HSP60 in the leptin-induced pancreatic protection has not been examined yet. The aim of this study was: to investigate the changes of HSP60 mRNA signal in the pancreatic AR42J cells subjected to caerulein and leptin. AR42J cells were incubated in standart medium at 37°C for: 0, 1, 3, 5, 12 or 24 h, under basal conditions. Incubation time of 3 h was selected for the next experiments. AR42J cells were incubated in presence of caerulein (10-11, 10-9 or 10-7M), leptin (10-8 or 10-6M), or combination of above. Gene expression for HSP60 was determined by RT-PCR. The mRNA signal for HSP60 has been observed in AR42J pancreatic cells under basal conditions. Incubation of AR42J cells in presence of leptin (10-8 or 10-6M) resulted in the significant increase of gene expression for HSP60 in both groups of AR42J cells. Caerulein stimulation reduced mRNA signal for HSP60. The strongest mRNA signal for HSP60 has been observed after the exposition of AR42J cells to combination of leptin and caerulein. We conclude that: 1. Gene expression for HSP60 has been detected in pancreatic AR42J cells under basal conditions. 2. HSP60 gene expression was significantly increased in pancreatic AR42J cells stimulated by leptin whereas caerulein reduced this signal. 3. The strongest gene expression for HSP60 has been detected in the cells incubated with combination of caerulein and leptin.

11

Increase of heat shock protein gene expression by melatonin in AR42J cells

51%

Bonior J., Jaworek J., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 3

Heat shock proteins (HSPs) have been reported to protect the pancreatic cells from the acute damage produced by caerulein overstimulation. However the effects of caerulein, melatonin or hyperthermia preconditioning on mRNA signal for HSP60 in the pancreatic acinar cells has not been examined yet. The aims of this study were: 1. To investigate the gene expression for HSP60 in the pancreatic AR42J cells stimulated by melatonin, caerulein or combination of both these substances. 2. To compare above changes with mRNA signal for HSP60 in pancreatic AR42J cells subjected to hyperthermia preconditioning. AR42J cells were incubated in standard medium at 37°C for: 0, 1, 3, 5, 12 or 24 h, under basal conditions. Above cells were then subjected to heat shock (42°C) for 0, 1 or 3 h. In the next part of the study AR42J cells were incubated in presence of caerulein (10-11, 10-9 or 10-7M), melatonin (10-8 or 10-6M), or combination of above under basal conditions or following heat shock pretreatment. Gene expression for HSP60 was determined by RT-PCR. The mRNA signal for HSP60 has been observed in AR42J cells under basal conditions, and this signal was markedly and time-dependently increased in these cells subjected to hyperthermia preconditioning. Incubation of AR42J cells in presence of melatonin (10-8 or 10-6M) resulted in the significant and dose-dependent increase of gene expression for HSP60 in both groups of AR42J cells: preconditioned and in those, which were not subjected to hyperthermia. Caerulein stimulation reduced mRNA signal for HSP60. The strongest signal has been observed after the exposition of AR42J cells to hyperthermia preconditioning, combined with melatonin and caerulein. We conclude that: 1. Gene expression for HSP60 has been detected in pancreatic AR42J cells under basal conditions. 2. Hyperthermia preconditioning resulted in a significant and time-dependent increase of HSP60 signal in pancreatic AR42J cells. 3. HSP60 gene expression was significantly increased in pancreatic AR42J cells stimulated by melatonin whereas caerulein reduced this signal. 4. The strongest gene expression for HSP60 has been found in the cells subjected to the combination of hyperthermia preconditioning, caerulein and melatonin.

12

Effects of ammonia and alkalization on secretory activity of isolated rat pancreatic acini

51%

Jaworek J., Jachimczak B., Konturek S. J.

Journal of Physiology and Pharmacology. Supplement

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1997

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tom 48

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nr 1

13

Only live Helicobacter pylori is capable of caspase-3 dependent apoptosis induction in gastric mucosa epithelial cells

45%

Pierzchalski P., Pytko-Polonczyk J., Jaworek J., Konturek S. J., Gonciarz M.

Journal of Physiology and Pharmacology

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2009

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tom 60

|

nr 4

119-128

Physiological process of cell death, apoptosis, plays a beneficial role in organism survival, but in some pathologies, like gastric Helicobacter pylori (Hp) infection, this process may turn against the host organism causing tissue damage. Knowledge of the mechanisms controlling apoptosis may have potential significance in treatment of these pathologic states. Therefore, we sought to determine whether apoptosis induced in the gastric epithelial cells exposed to live Hp involves the alteration in heat shock protein 70 (HSP70) expression and activation of caspase-3 in peroxisome proliferator-activated receptors (PPARg dependent manner). Experiments were performed with KATO III, gastric epithelial cells, exposed to CagA and Vac A positive live Hp , water Hp extracts or Hp culture supernatant over different time periods. Total cellular RNA and proteins were isolated for PCR, western-blot and EMSA studies. Genomic DNA was isolated to analyze apoptosis status. We propose new model of Hp induced HSP70 dependent, caspase-3 executed apoptosis in human gastric epithelium. KATO III cells exposed to Hp , showed an increase in caspase-3 activity accompanied and preceeded by activation of nuclear translocation of PPAR peaking at 48 h of culture. Moreover, heat shock factor 1 (HSF-1) bound up with phosphorylated STAT-3 was unable to activate HSP70 protein synthesis in KATO III exposed to Hp . Lack of protective effect of HSP70, activation of caspase-3 - dependent apoptosis pathway caused by Hp and alteration of the bax/bcl-2 cellular equilibrium led to gastric epithelial cell death. The observed phenomenon might be helpful in understanding of the mechanism of Hp related gastrointestinal tract diseasess, especially gastric cancer.

14

Interleukin-25, thymic stromal lymphopoietin and house dust mites in pathogenesis of atopic dermatitis

45%

Jaworek A. K., Szafraniec K., Zuber Z., Wojas-Pelc A., Jaworek J.

Journal of Physiology and Pharmacology

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2020

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tom 71

|

nr 2

15

Endotoxemia in the infant rats modulates HSP60 protein level in the pancreatic acinar cells

45%

Bonior J., Jaworek J., Kot M., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

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nr 3

189-198

16

Effect of intravenous administration of amino acids and lipids on pancreatic secretion

45%

Jaworek J., Tasler J., Cieszkowski M., Mitis M., Konturek S. J.

Acta Physiologica Polonica

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1979

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tom 30

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nr 1

17

Melatonin stimulates HSP27 phosphorylation in human pancreatic carcinoma cells [PANC-1]

45%

Leja-Szpak A., Jaworek J., Szklarczyk J., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

|

nr 3

177-188

18

Role of calcitonin gene related peptide in the modulation of intestinal circulatory, metabolic and myoelectric activity during ischemia-reperfusion

45%

Pawlik W. W., Obuchowicz R., Biernat J., Sendur R., Jaworek J.

Journal of Physiology and Pharmacology

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2000

|

tom 51

|

nr 4,2

19

Brain-gut axis in ischemic preconditioning of gastro-duodeno-pancreatic cluster

45%

Konturek S. J., Brzozowski T., Pajdo R., Dembinski A., Jaworek J.

Journal of Physiology and Pharmacology. Supplement

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2001

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tom 52

|

nr 2

20

Leptin is able to stimulate pancreatic enzyme secretion via activation of duodeno-pancreatic reflex and CCK release

39%

Nawrot-Porabka K., Jaworek J., Leja-Szpak A., Palonek M., Szklarczyk J., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

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2004

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tom 55

|

nr 2

47-57

Leptin, 16- kDa protein produced and secreted from white adipocytes is known to regulate food intake and energy expenditure. Leptin receptors have been detected in the pancreas and it has been shown that systemic application of this protein diminished postprandial pancreatic secretion. Leptin is also produced in the stomach and released into the gastrointestinal lumen but the implication of luminal leptin in the regulation of pancreatic enzyme secretion has not been elucidated. The aim of our study was to evaluate the effects of intraduodenal (i.d.) leptin administration on pancreatic enzyme secretion and to assess the involvent of afferent nerves and CCK in above effects. The secretory studies were carried out on anaesthetized Wistar rats with acute pancreatic fistulae. Leptin was administered to the animals at doses of 0.1 1.0 or 10.0 µg/kg i.d. Tarazepide (2.5 mg/kg i.d.), a CCK1 receptor antagonist, was given to the rats prior to the application of leptin. Rats with capsaicin deactivated sensory nerves were used in part of the study. Samples of pancreatic juice were taken at 15 min intervals to measure the volume flow and protein and amylase concentrations. CCK plasma level was measured by radioimmunoassay (RIA) following administration of leptin to the rats. Intraduodenal administration of leptin (1.0 or 10.0 µg/kg) to the fasted rats significantly and dose-dependently increased pancreatic protein and amylase outputs. Pancreatic secretory responses to leptin were totally abolished by prior capsaicin deactivation of sensory nerves or by pretreatment of the rats with tarazepide. Under basal conditions plasma CCK level averaged about 15.46 ± 1,4 pg/ml. Exogenous leptin, given i.d. at doses of 0.1 1.0 or 10.0 µg/kg i.d. to the rats with intact or capsaicin-deactivated sensory nerves resulted in dose-dependent rise of plasma CCK level, reaching the highest value at the dose of 10.0 µg/kg i.d. We conclude that leptin given i.d. stimulates pancreatic enzyme secretion and this effect could be related to the stimulation of CCK release and activation of duodeno-pancreatic reflexes.

Ograniczanie wyników

Rola społeczeństwa w ochronie i zarządzaniu krajobrazem na przykładzie Caerlaverock i Langholm and Newcastleton Hills w południowej Szkocji

Ghrelin and melatonin in the regulation of pancreatic exocrine secretion and maintaining of integrity

Central and peripheral control of pancreatic integrity by leptin and melatonin

Effects of prostaglandin of E, F and I series, leukotriene C4 and platelet activating factor on amylase release from isolated rat pancreatic acini

Zmiana powierzchni zbiorowisk o charakterze leśnym w dolinie Karpnickiego Potoku jako przykład zmian lesistości w dolnych partiach Sudetów

Brain-gut axis in the modulation of pancreatic enzyme secretion

Krajobraz doliny Karpnickiego Potoku. Zastosowanie systemow GIS w analizach historycznych krajobrazu

Effect of enkephalin and morphine on pancereatic secretion

Rozmieszczenie zasobów drzew z rodzaju wiąz (Ulmus spp.) w lasach Polski w świetle dokumentacji leśnej

Leptin is the modulator of HSP60 gene expression in AR42J cells

Increase of heat shock protein gene expression by melatonin in AR42J cells

Effects of ammonia and alkalization on secretory activity of isolated rat pancreatic acini

Only live Helicobacter pylori is capable of caspase-3 dependent apoptosis induction in gastric mucosa epithelial cells

Interleukin-25, thymic stromal lymphopoietin and house dust mites in pathogenesis of atopic dermatitis

Endotoxemia in the infant rats modulates HSP60 protein level in the pancreatic acinar cells

Effect of intravenous administration of amino acids and lipids on pancreatic secretion

Melatonin stimulates HSP27 phosphorylation in human pancreatic carcinoma cells [PANC-1]

Role of calcitonin gene related peptide in the modulation of intestinal circulatory, metabolic and myoelectric activity during ischemia-reperfusion

Brain-gut axis in ischemic preconditioning of gastro-duodeno-pancreatic cluster

Leptin is able to stimulate pancreatic enzyme secretion via activation of duodeno-pancreatic reflex and CCK release