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Aspects of red soil properties and water management in China

100%

Cao Z., Zhu X.

International Agrophysics

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1999

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tom 13

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nr 1

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The endomycorrhizal fungal species and their effects on the growth and nutrient characteristics of Eucalyptus maidenii seedlings in China

71%

Wang X., Kong X., Zhao Y., Cao Y., Cao Z.

Dendrobiology

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2020

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tom 83

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Identification of Ephedra species by phylogenetic analyses using matK and ITS1 sequences

61%

Zhao Y. S., Xie L. X., Mao F. Y., Cao Z., Wang W. P., Zhao Q. P., Zhang X. H.

Acta Societatis Botanicorum Poloniae

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2016

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tom 85

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nr 2

In this study, the species identifications of seven Ephedra plants, including three medicinal plants from the Pharmacopoeia of the People’s Republic of China, were conducted using phylogenetic analyses, and the method’s validity was verified. The phylogenetic trees constructed from the maturase-coding gene (matK) and internal transcribed spacer 1 (ITS1) sequences showed that the former could be used for identifying five Ephedra plants, Ephedra intermedia, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. przewalskii, while the latter could distinguish five Ephedra plants, E. przewalskii, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. intermedia. However, when the two genes were combined, the seven species could be completely distinguished from each other, especially the medicinal plants from the others, which is significant in developing their pharmaceutical uses and in performing quality control assessments of herbal medicines. The method presented here could be applied to the analysis of processed Ephedra plants and to the identification of the botanical origins of crude drugs. Additionally, we discovered that E. equisetina and E. major were probably closely related to each other, and that E. sinica, E. intermedia, and E. przewalskii also had a close genetic relationship.

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Involvement of haem oxygenase-1 in hydrogen peroxide-induced lateral root formation in tomato

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Cao Z., Fang T., Chen M., Li J., Shen W., Huang L.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 04

The objective of this study was to test whether haem oxygenase-1 (HO-1) is involved in hydrogen peroxide (H₂O₂)-induced lateral root (LR) formation. The results showed that 0.1 mM H₂O₂ mimicked the effects of the HO-1 inducer, haemin, on the up-regulation of tomato HO-1 (SlHO1) expression, increased carbon monoxide (CO) synthesis and LR formation. However, 1.0 mM H₂O₂ resulted in inhibitory responses. The above inducible or inhibitory responses elicited by 0.1 and 1.0 mM H₂O₂ were noticeably blocked or rescued by the HO-1 inhibitor zinc protoporphyrin IX (ZnPP) or haemin, and then separately reversed by CO or ZnPP. Further trials showed that haemin-induced responses were not altered by the H₂O₂ trap, dimethylthiourea (DMTU). When applied alone, DMTU not only decreased H₂O₂ contents but also inhibited SlHO1 expression and LR development. These responses were recovered by the application of haemin or CO. Molecular evidence revealed that H₂O₂-modulated expression of the target genes responsible for LR formation was blocked by ZnPP, but rescued by CO. Salinity-induced up-regulation of HO-1 expression and thereafter LR formation were also dependent on the H₂O₂ generation. Overall, these results demonstrated a possible role of HO-1 in the H₂O₂-induced tomato LR formation.

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Improved fusion protein expression in EGFP via the mutation of both Kozak and the initial ATG codon

61%

Dai C., Cao Z., Wu Y., Yi H., Jiang D., Li W.

Cellular and Molecular Biology Letters

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2007

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tom 12

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nr 3

Since its discovery, green fluorescence protein (GFP) has been used as a reporter in a broad range of applications, including the determination of gene expresion in diverse organisms, and subcellular protein localization. pEGFP-N1 is a eukayotic expression vector encoding EGFP, the MCS of which locates at the N terminus of EGFP. In this study, the cDNA sequence of scorpion toxin BmKK2 was inserted into the XhoI-HindIII cut of pEGFP-N1 to construct a toxin-EGFP fusion gene (named pEGFP-BmKK2). Fluorescence imaging revealed that HEK 293T cells that were transfected by pEGFP-BmKK2 emitted green fluorescence. Transcription of pEGFP-BmKK2 was confirmed by RT-PCR. However, western blotting analysis showed that the transfected HEK 293T cells expressed mostly EGFP, but little toxin-EGFP fusion protein, implying that pEGFP-N1 cannot be used as a fusion expression vector for subcellular protein localization for the BmKK2 gene. Consequently, two modified recombinant vectors (pEGFP-BmKK2-M1 and pEGFP-BmKK2-M2) were constructed based on pEGFP-BmKK2. This greatly improved the expression of toxin-EGFP fusion protein from pEGFP-BmKK2-M2.

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Structure and evolution of the complete mitochondrial genome of the freshwater drum, Aplodinotus grunniens (Actinopterygii: Perciformes: Sciaenidae)

51%

Wen H., Ma X., Xu P., Cao Z., Zheng B., Hua D., Jin W., Sun G., Gu R.

Acta Ichthyologica et Piscatoria

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