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The objective of this present investigation was to study the different response on drought tolerance and post-drought recovery for two different genotypes of white clover (a drought-tolerant small-leafed white clover and a drought-sensitive large-leafed white clover) associating with antioxidative enzyme and lignin metabolism. Two materials were exposed to 12 days of drought stress and 6 days of rewatering in a greenhouse. Significantly higher activities of superoxide dismutase, ascorbate peroxidase, catalase, guaiacol peroxidase and root activity as well as lower electrolyte leakage and malondialdehyde levels of the small-leafed in leaves and roots were observed as compared to the large-leafed under drought stress and rewatering. Lignification-related enzymes (polyphenol oxidase, cinnamyl alcohol dehydrogenase) activities of two materials in leaves gradually increased from 0 to 9 days of drought stress; then declined at 12 days of drought stress and after rewatering. After 12 days of drought stress, lignin content of two materials in roots was significantly higher relative to the well-watered. Lignin content of leaves in the small-leafed also increased significantly after 6 days of rewatering, but not in the large-leafed. The small-leafed exhibited more sensitive lignin metabolism and faster lignification in leaves and roots than the large-leafed regarding the response of drought stress. These results indicated that antioxidant efficiency and lignin metabolism played important roles in response to drought stress and rewatering for different white clovers. Comparatively higher reactive oxygen species scavenging system and more sensitive lignin metabolism could be associated with better drought tolerance and post-drought recovery in white clover.
Soil erosion is one of the most important environmental concerns in the hilly and gully region of the Chinese Loess Plateau. In this study, a distributed soil erosion model considering the shallow gully erosion (ephemeral gully erosion) was used to simulate and track soil erosion changes in an easily-eroded watershed from 1985 to 2010. Results indicate that: 1) The distributed soil erosion model based on the RUSLE is suitable for the hilly and gully region of the Loess Plateau and can better improve modeling capabilities for future study of sediment and pollution. 2) The overall soil erosion intensity in the southeast and central parts of the watershed is higher than that of the northwest. The spatiotemporal variations of soil erosion in the whole watershed are largely related to rainfall erosion distribution and land use layout. 3) Effects of returning farmland measures on soil erosion in the Yanhe River upstream are remarkable, and the Panlong River basin needs to further strengthen soil and water conservation measures, and the Yanhe downstream cannot also be ignored in future watershed management planning. 4) Dry land is the critical source area before and after returning farmland in the watershed. The implementation of soil and water conservation measures of dry land is a top priority for soil erosion prevention and control of the watershed. Results may provide scientific reference for erosion identification of critical source areas and land use planning in the loess hilly and gully region.
The verneuiliid brachiopod Changshunella yangi gen. et sp. nov. is described from the Early Carboniferous Muhua Formation of Guizhou, China. This rather unusual form is characterized by having very small shell dimensions (2.6–4 mm in length), a very high flat procline ventral interarea, and by having three prominent plications on both valves that are arranged in opposed folding; the last feature is characteristic for the family Verneuiliidae. We suggest that the family has been derived from an offshoot of the stock that produced the superfamilies Spiriferoidea, Paeckelmanelloidea, and Brachythyridoidea by developing opposed folding and loss of dental plates or dental adminicula as exemplified by Verneuilia and Changshunella gen. nov.
We present taxonomic revision of rugose corals and brachiopods from several Frasnian–Fammenian (F–F) boundary sections in central Hunan Province, China. Diversity of shallow−water rugose corals gradually increased during the Frasnian, but ended with sudden extinction near the end of Frasnian. Ostracods were abundant during the Frasnian; their extinction coincided with anoxic deposition of the end−Frasnian black shale deposits. The early Famennian ostracod fauna is of low diversity. The brachiopod fauna of the late Frasnian (Palmatolepis rhenana and Pa. linguiformis zones) is dominated by atrypids, small−sized cyrtospiriferids, and the rhynchonellid Hunanotoechia. All atrypids disappeared before the F–F boundary with highest rates of extinction below the boundary (probably low in the Pa. linguiformis Zone). The Frasnian cyrtospiriferid fauna is also of low diversity and dominated by small taxa. All but one of the cyrtospiriferid taxa crossed the F–F boundary. The early Famennian post−extinction recovery brachiopod fauna was the result of rapid radiation of new forms shortly after the terminal Frasnian event. The early Famennian fauna is characterized by diverse cyrtospiriferids, abundant Yunnanellina and productoids. Above the early recovery fauna another fauna was recovered, with brachiopods Hunanospirifer and Yunnanella and is correlated with the late or latest Pa. crepida Zone. Sinalosia rugosa gen. et sp. nov. (Productida) is erected.
Although some genes involved in heavy metal detoxification and tolerance have been identified in plants, the mechanisms underlying heavy metal resistance remain to be elucidated further. To investigate its molecular mechanisms, a forward genetic screen was performed for identifying Arabidopsis (Arabidopsis thaliana) cadmium (Cd)-sensitive mutants. Here we report a novel cms1-1 (cadmium-sensitive) mutant, which defines a genetic locus involved in the regulation of cadmium and lead (Pb) resistance in Arabidopsis. cms1-1 plants were hypersensitive to Cd but resistant to Pb as well as oxidative stress mediated by hydrogen peroxide and methyl viologen. Genetic analysis indicated that cms1-1 is a recessive mutation in a single nuclear gene. When subjected to Cd or Pb stress, higher Cd or lower Pb content was detected in cms1-1 plants than in wild-type plants, respectively. The cms1-1 mutation altered the expression pattern of some Cd/ Pb stress-related genes, including AtPDR8 and AtPDR12, which was associated with changes of Cd and Pb contents. In addition, Cd hypersensitivity/enhanced Pb resistance mediated by cms1-1 is glutathione (GSH)- independent. The results suggest that CMS1 plays distinct roles in the regulation of Cd and Pb resistance mainly through a GSHindependent AtPDR12/AtPDR8-mediated mechanism.
The foraging behavior of greater short-nosed fruit bats (Cynopterus sphinx) on wild banana (Musa acuminata) and subsequent dispersal of seeds were studied in the Tropical Rainforest Conservation Area, Xishuangbanna Tropical Botanical Garden, Yunnan province, by direct observation of marked fruits, mist netting, and seed collection. The mean number (± SE) of individual C. sphinx captured by mist net were 2.2 ± 0.33/day and 1.4 ± 0.32/day in the rainy season (September to October) and dry season (November to December), respectively; the difference was not significant. The number of seed pellets expelled was 9.0 ± 1.12/day and 7.2 ± 1.37/day in the rainy and dry seasons respectively; again the difference was not significant. The removal curves for marked fruit were similar for 10 focal trees. Fruits were consumed heavily within two weeks after ripening and all the marked fruit were removed within one month. The difference in seed dispersal was significant between different feeding roosts indicating that patterns of seed dispersal may not be uniform. We found the seeds of M. acuminata can be dispersed by C. sphinx to a distance of about 200 m, and C. sphinx can be considered as an effective seed disperser of M. acuminata.
Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.
The cucurbitane-type triterpenoid glycosides, mogrosides, are the main active components of Siraitia grosvenorii fruit. Squalene and cucurbitadienol are among the intermediates of the biosynthetic pathway for the formation of cucurbitane-type triterpenoid backbones of mogrosides. It is recognized that the exogenous application of methyl jasmonate (MeJA) increases the accumulation of secondary metabolites in various plant species. Here, the effect of MeJA (50, 200, and 500 μM) on the accumulation of squalene and cucurbitadienol in the fruits of S. grosvenorii at 10, 20, and 30 days after flowering (DAF) was tested for the first time. Since mogroside II E is the main cucurbitane-type triterpenoid present at this time, its concentration was also determined. The results show that MeJA can indeed promote squalene and cucurbitadienol accumulation, the application of 500 μM MeJA at 30 DAF being optimal. The concentration of squalene and cucurbitadienol increased up to 0.43 and 4.71 μg/g dry weight (DW), respectively, both of which were 1.2-fold greater than that of the control. The content of mogroside II E increased by 15% over the untreated group. We subsequently analyzed the expression of key genes involved in the mogroside biosynthetic pathway, including the 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene (SgHMGR), squalene synthetase gene (SgSQS), cucurbitadienol synthase gene (SgCS), and cytochrome P450 (SgCYP450) with quantitative real-time PCR. The results showed that transcriptional levels of these genes were upregulated following the treatment described above. Additionally, their responses in the presence of MeJA was related to the concentration and timing of MeJA treatment.
A semi-field test was conducted to assess the risk of exposure to fufenozide in a ditch and pond adjacent to an agricultural area. To support the investigation, a fast, highly selective, and sensitive method was developed to determine the residue of fufenozide in water, sediment, and soil through high performance liquid chromatography- tandem mass spectrometry. The recoveries were in the acceptable range of 85.6% to 99.3% in the three matrices, with the associated relative standard deviations at 1.2% to 7.8%. The results indicate that the surface water-sediment system could be exposed to fufenozide through runoff after application, which dissipated rapidly in the aquatic ecosystem. The toxicity exposure ratio showed no risk of fufenozide exposure to the fish in the aquatic ecosystem close to the agriculture field.
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