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Bacterial contamination of indoor air, surfaces, and settled dust, and related dust endotoxin concentrations in healthy office buildings

100%

Bouillard L., Michel O., Dramaix M., Devleeschouwer M.

Annals of Agricultural and Environmental Medicine

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2005

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tom 12

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nr 2

187-192

Endotoxin, a characteristic external fraction of the outer membrane from Gram-negative bacteria, continuously shed into the environment, is considered as an important risk factor for human health. Our purpose was to study the bacterial species contaminating healthy working environments. Airborne, working surfaces and carpet dust samples were collected from 25 offices. Bacterial species were identified with biochemical ApiSystem® strips. Endotoxin concentrations in settled dust were measured with the kinetic chromogenic Limulus assay. The airborne bacterial level varied from 44-2,511, with a median of 277 cfu/m3. Bacterial contamination on surfaces ranged from 1-1000, with 33 cfu/25 cm2 as median value. On carpets, bacterial concentration ranged from 0.73-185 x 105 cfu/g, with 7.28 x 105 cfu/g as median value. Endotoxin concentration varied from 4.6-116.2 EU/mg, with a median of 20.3 EU/mg. Altogether, 501 bacterial strains were isolated. The species variability was greater in Gram-negative bacteria than in Gram-positive cocci with 41 versus 34 various species. In conclusion, people working in healthy offices can be exposed to large concentrations of airborne and dust bacteria and related endotoxin concentrations, giving a risk of work-related diseases.

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Podstawy elektrochemioterapii w onkologii i chirurgii weterynaryjnej

100%

Tunikowska J., Kulbacka J., Michel O., Kielbowicz Z.

Weterynaria w Praktyce

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2018

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tom 15

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nr 06

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In vitro study of the effect of corn dried distillers grains with solubles on rumen fermentation in sheep

76%

Pecka-Kielb E., Zawadzki W., Zachwieja A., Michel O., Mazur M., Mista D.

Polish Journal of Veterinary Sciences

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2015

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tom 18

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nr 4

The aim of the in vitro study was to determine the effect of corn dried distillers grains with solubles (corn DDGS), used as a replacement for the concentrate ingredients of sheep diet, on rumen fermentation. The material for the study was the ruminal fluid of Polish Merino sheep which was incubated during 4-, 8- or 24-hour periods. Five groups of samples were prepared for in vitro fermentation: C – control, incubated with the substrate consisting of the concentrate ingredients; D1, D2 and D3, where DDGS was used as a substrate added in proportions of 10, 20 and 30% of dry matter of the concentrate; and D4, where 100% DDGS was used as a substrate. After fermentation, the gas and short chain fatty acids (SCFAs) analyses were performed using gas chromatography. The ammonia concentration and pH were also determined, and the SCFA utilization index (NGR), the fermentation efficiency (FE) and the index of cell yield of ruminal microorganisms (CY) were calculated. This research showed no effect of DDGS on the methane emission. The positive correlations between the amount of methane and ammonia concentrations in the 8- and 24-hour fermentation periods were found. DDGS addition increased propionate proportion, but decreased production of acetate (p<0.01). Additionally, D1, D2, D3 and D4 substrates lowered isobutyrate (p<0.05) and isovalerate (p<0.01) production. Based on the results obtained, it can be stated that partial substitution of the concentrate ingredients with DDGS did not have deleterious effect on sheep rumen fermentation processes.

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The favourable effect of catechin in electrochemotherapy in human pancreatic cancer cells

76%

Michel O., Przystupski D., Saczko J., Szewczyk A., Niedzielska N., Rossowska J., Kulbacka

Acta Biochimica Polonica

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2018

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tom 65

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nr 2

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Second inter-laboratory study comparing endotoxin assay results from cotton dust

51%

Chun D. T. W., Chew V., Bartlett K., Gordon T., Jacobs R. R., Larsson B. M., Lewis D. M., Liesivouri J., Michel O., Rylander R., Thorne P. S., White E. M., Gunn V. C., Wurtz H.

Annals of Agricultural and Environmental Medicine

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2002

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tom 09

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nr 1

Ograniczanie wyników

Bacterial contamination of indoor air, surfaces, and settled dust, and related dust endotoxin concentrations in healthy office buildings

Podstawy elektrochemioterapii w onkologii i chirurgii weterynaryjnej

In vitro study of the effect of corn dried distillers grains with solubles on rumen fermentation in sheep

The favourable effect of catechin in electrochemotherapy in human pancreatic cancer cells

Second inter-laboratory study comparing endotoxin assay results from cotton dust