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1

W szubie, ale z odkrytą...

100%
Pniewski T.
Głos Lasu
|
2014
|
nr 07-08
2

Expression of HBV core antigen (HBcAg) in transgenic tobacco and lettuce for purposes of therapeutic vaccine against hepatitis B

84%
Bogusiewicz M., Pniewski T.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
|
tom 51
|
nr 1
3

Efficiency of transformation of Polish cultivars of pea [Pisum sativum L.] with various regeneration capacity by using hypervirulent Agrobacterium tumefaciens strains

84%
Pniewski T., Kapusta J.
Journal of Applied Genetics
|
2005
|
tom 46
|
nr 2
An Agrobacterium-mediated transformation method of pea has been developed for several edible and fodder cultivars of pea (Pisum sativum L.), characterized previously in their potential for regeneration via organogenesis) The most appropriate explant, which was susceptible to Agrobacterium infection and capable of regenerating transgenic plants, turned out to be a slice of an immature embryo, including the embryo axis and the basal part of a cotyledon. Three hypervirulent strains of A. tumefaciens were tested: AgL0, AgL1 and EHA105. Each carried the binary vector pP35SGIB containing the uid gene, with an intron under control of the 35S promoter, and the bar gene conferring resistance to phosphinotricin. Strain AgL0 was found to be efficient for the majority of cultivars, followed by AgL1 and EHA105. Transformation efficiency varied from 0.7 to 4.1%, depending on cultivar and Agrobacterium strain. The transformation efficiency of particular pea cultivars did not clearly correspond to their regeneration capacity, which - although indispensable - was not a critical parameter of successful transformation. The presence of integrated genes in pea genomic DNA was detected by the PCR. T-DNA was stably transmitted to the progeny, as it was confirmed by Southern hybridization. The activity of introduced genes was analysed by the histochemical GUS assay and by painting leaves or by spraying transgenic plants with the herbicide Basta.
4

Transformation of microspore-derivated of winter oilseed rape [Brassica napus L.] by using Agrobacterium tumefaciens

68%
Cegielska-Taras T., Pniewski T., Szala L.
Journal of Applied Genetics
|
2008
|
tom 49
|
nr 4
343-347
Haploid microspore-derived embryos (MDEs) constitute a unique material for the introduction of new traits into winter oilseed rape (Brassica napus). MDEs have been transformed by using Agrobacterium tumefaciens strains EHA105 and LBA4404, both carrying the binary vector pKGIB containing the uidA gene encoding -glucuronidase (GUS) and the bar gene as a marker of resistance to phosphinotricin. Transformed embryos expressed GUS and regenerated plants that were resistant to herbicide Basta, as confirmed by a leaf-painting test. Progeny plants of the transformant T-39 were all transgenic, as they inherited T-DNA from their doubled haploid parental plant. Southern-blot analysis confirmed the integration and transmission of T-DNA into T₁ plants. Transformation of MDEs facilitates the obtaining of winter oilseed rape homozygous for the introduced genes.
5

HBV core antigen (HBcAg) as a plant-based prototype of therapeutic hepatitis B vaccine and a carrier for HBV preS epitopes

68%
Pniewski T., Bogusiewicz M., Wyrwa K., Czyz M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
|
tom 51
|
nr 1
6

Improved production of doubled haploids of winter and spring triticale hybrids via combination of colchicine treatments on anthers and regenerated plants

68%
Slusarkiewicz-Jarzina A., Pudelska J., Wozna J., Pniewski T.
Journal of Applied Genetics
|
2017
|
tom 58
|
nr 3
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Organogenesis and long-term micropropagation of Polish pea cultivars

68%
Pniewski T., Wachowiak J., Kapusta J., Legocki A. B.
Acta Societatis Botanicorum Poloniae
|
2003
|
tom 72
|
nr 4
The complete protocol for regeneration and long-term micropropagation of several Polish cultivars of pea (Pisum sativum L.) has been elaborated. The shoots were the most likely regenerated via de novo organogenesis. The adventitious buds formed in callus derived from cotyledons tissue adjacent to the axillary meristems of immature embryos. All cultivars' calli regenerated several shoots per explant on the MS medium supplemented with B5 vitamins and 4.5 mgl-1 of BAP, however some differences in regeneration capacity among cultivars were observed. The plantlets were subsequently micropropagated with slightly higher efficiency and preserving a good viability over the long-term culture on a medium containing 2.0 mgl-1 than one with 4.5 mgl-1 of BAP. The additional step of the pre-conditioning culture of multiplicated shoots on a medium with very low BAP concentration i.e. 0.02 mgl-1 was applied and appeared to be beneficial before rooting in vitro or grafting. The modified MS-derived medium with the half-strength of MS macroelements but with the full original dose of calcium and supplemented with B5 vitamins and 1.0 mgl-1 of NAA was developed for effective rooting. The shoots were also sufficiently transferred into ex vitro conditions using grafting. The majority of the regenerated plants had adapted to in vivo conditions in a greenhouse and subsequently has set seeds. The presented protocol provides relatively efficient rate of de novo pea regeneration and would be useful for Agrobacterium-mediated transformation purposes.
8

The expression of E2 antigens of classic swine fever virus [CSFV] in transgenic plants

68%
Miedzinska K., Pniewski T., Legocki A. B.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
9

In vitro micropropagation of four lupin species

68%
Pniewski T., Kapusta J., Legocki A. B.
Acta Physiologiae Plantarum
|
2002
|
tom 24
|
nr 4
10

Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture

68%
Pniewski T., Kapusta J., Plucienniczak A.
Journal of Applied Genetics
|
2006
|
tom 47
|
nr 4
The idea of an oral vaccine administered as a portion of plant tissue requires a high level of antigen production. An improved protocol for the induction of transgenic yellow lupin calli or tumours, reaching 44% of transformation rate, is presented here. It has been developed by using the nptII marker gene and the uidA reporter gene as well as various Agrobacterium strains and plant explants. This method of seedling and hypocotyl transformation was applied to raise calli or tumours producing a small surface antigen of Hepatitis В Virus (S-HBsAg). Lupin tissue lines were long-term cultured on selection media maintaining the growth rate and high expression level of the native form of S-HBs, up to 6 µg per g of fresh tissue.
11

Nanogram doses of alum-adjuvanted HBs antigen induce humoral immune response in mice when orally administered

60%
Kapusta J., Pniewski T., Wojciechowicz J., Bociag P., Plucienniczak A.
Archivum Immunologiae et Therapiae Experimentalis
|
2010
|
tom 58
|
nr 2
143-151
12

Plant regeneration in the in vitro cultures of the selected species of Miscanthus

51%
Slusarkiewicz-Jarzina A., Ponitka A., Cerazy J., Pniewski T., Pudelska H., Malec K., Jezowski S.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
|
nr 2
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Expression of hepatitis B core antigen in lettuce and tobacco for advanced plant-derived vaccine formulations

51%
Pyrski M., Kasprzyk A., Bogusiewicz M., Bociag P., Czyz M., Pniewski T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Agrobacterium-mediated genetic transformation of Miscanthus sinensis and Miscanthus × giganteus. Preliminary studies

51%
Cerazy J., Slusarkiewicz-Jarzina A., Ponitka A., Malec K., Jezowski S., Pniewski T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
15

Identyfikacja i charakterystyka ekspresji genów determinujących cechy związane z plonowaniem w kombinacjach krzyżówkowych grochu

51%
Krajewski P., Bocianowski J., Gawlowska M., Kaczmarek Z., Pniewski T., Swiecicki W., Wolko B.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2006
|
nr 240-241
271-278
W pracy przedstawione są wstępne wyniki badań prowadzonych na podstawie linii uzyskanych z kombinacji krzyżówkowych Wt11238 × Wt3557 i Wt10245 × Wt11238 grochu Pisum sativum L., zmierzających do opisu działania loci determinujących cechy związane z plonowaniem. Podane są rezultaty doświadczenia jednorocznego prowadzonego w dwu środowiskach. Opisana jest zastosowana metoda statystyczna mapowania loci cech ilościowych, która uwzględnia sposób otrzymania linii i pozwala na ocenę efektów addytywnego działania genów oraz dominacji.
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The expression of HBV antigens in transgenic lettuce micropropagated in vitro cultures for the purpose of anti-HBV vaccine

43%
Czyz M., Wyrwa K., Bociag P., Dembczynski R., Marecik R., Krajewski P., Kapusta J., Pniewski T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Activity of hygromycin phosphotransferase marker gene optimized for expression in plants for the construction of vectors for genetic modifications of grasses

43%
Malec K., Kowalska M., Podkowinski J., Zielinska M., Cerazy J., Slusarkiewicz-Jarzina A., Ponitka A., Jezowski S., Pniewski T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
18

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

35%
Pniewski T., Kapusta J., Bociąg P., Wojciechowicz J., Kostrzak A., Gdula M., Fedorowicz-Stronska O., Wojcik P., Otta H., Samardakiewicz S., Wolko B., Plucienniczak A.
Journal of Applied Genetics
|
2011
|
tom 52
|
nr 2
Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors and the optimization of vaccine compositions and administration protocols. Previous immunization studies were mainly based on a combination of the injection of a small surface antigen of HBV (S-HBsAg) and the feeding with raw tissue containing the antigen, supplemented with an adjuvant, and coming from plants conferring resistance to kanamycin. The objective of this study was to develop a prototype oral vaccine formula suitable for human immunization. Herbicide-resistant lettuce was engineered, stably expressing through progeny generation micrograms of S-HBsAg per g of fresh weight and formed into virus-like particles (VLPs). Lyophilized tissue containing a relatively low, 100-ng VLP-assembled antigen dose, administered only orally to mice with a long, 60-day interval between prime and boost immunizations and without exogenous adjuvant, elicited mucosal and systemic humoral anti-HBs responses at the nominally protective level. Lyophilized tissue was converted into tablets, which preserved S-HBsAg content for at least one year of room temperature storage. The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B.
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