Wyniki wyszukiwania

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RAPD analysis of somaclonal and natural DNA variation in Hypericum perforatum L.

100%

Haluskova J., Kosuth J.

Acta Biologica Cracoviensia. Series Botanica

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2003

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tom 45

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nr 2

RAPD analysis of DNA variation in somaclones of Hypericum perforatum which passed through one or two cycles of in vitro regeneration revealed great variation among the donor plants from which the somaclones were regenerated. The donor plants represented either seed-derived plants or the seed progeny of first-cycle somaclones. The variation among them may indicate natural genetic variation in Hypericum perforatum. In addition, no differences were found in the RAPD profiles of 51 first-cycle somaclones within groups originated from 7 donor plants. This may point to relative stability of the Hypericum perforatum genome under given conditions of in vitro regeneration. One polymorphic band was found in 3 of 51 RAPD profiles of second-cycle somaclones, perhaps an effect of repeated in vitro regeneration on DNA changes. Study of DNA variation among 75 Hypericum perforatum progenies derived from 8 mother plants indicates the prevalence of the apomictic mode of reproduction. Analysis of 47 seed progenies of diploid somaclones derived from 5 R3 plants may suggest the sexual mode of reproduction.

2

Morphogenetic response to plant growth regulators in transformed and untransformed Hypericum perforatum L. clones

71%

Koperdakova J., Katkovcinova Z., Kosuth J., Giovannini A., Cellarova E.

Acta Biologica Cracoviensia. Series Botanica

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2009

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tom 51

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nr 1

61-70

This study examined the effects of different exogenous auxins and cytokinins at 0.1-5.0 mg·l-1 concentration on shoot cuttings of two H. perforatum clones transformed with a wild agropine strain of A. rhizogenes and one untransformed clone. Their sensitivity to the auxins varied and showed concentration-dependent behavior, and the response to auxins differed between the transgenic clones. The number of cuttings of transgenic clones capable of root formation, and the onset of rooting on most of the media with auxins lagged behind the control. The number of differentiated shoots of the transgenic clones on hormone-free medium was two to three times higher than that of the untransformed control. Regenerated shoots of the transgenic clones on basal medium branched much less than the nontransgenic clone. The transgenic and control clones differed in their ability to form shoots on media supplemented with cytokinins. Increased cytokinins led to differentiation of shorter shoots with fewer leaf pairs. Because gene expression studies have shown integration of rolABC genes, their possible impact on the type of morphogenetic response is discussed.

3

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

71%

Koperdakova J., Komarovska H., Kosuth J., Giovannini A., Cellarova E.

Acta Physiologiae Plantarum

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2009

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tom 31

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nr 2

Hairy root-regenerated clones of Hypericum perforatum L. grown in vitro similarly to those successfully adapted to ex vitro conditions showed phenotype features typical for plants transformed with Agrobacterium rhizogenes T-DNA. These included reduced apical dominance, increased branching, dwarfing and reduced fertility. Transgenic clones differed in ability to develop root system as a necessary condition for transfer to the soil. One of the profiling characters, capability of hypericin biosynthesis was altered as well. Dark glands as the sites of hypericin accumulation and/or synthesis exhibited significantly higher densities on both, leaves and petals of transgenic clones comparing to controls. In the genome of transgenic clones, rolABC genes were detected. Both clones harboured similar copy number of individual rol genes. However, copy numbers descended from rolA to rolC gene in both clones.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

1 Acta Physiologiae Plantarum

3 Kosuth J.

2 Cellarova E.

2 Giovannini A.

2 Koperdakova J.

1 Haluskova J.

1 Katkovcinova Z.

1 Komarovska H.

2 2009

1 2003

RAPD analysis of somaclonal and natural DNA variation in Hypericum perforatum L.

Morphogenetic response to plant growth regulators in transformed and untransformed Hypericum perforatum L. clones

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones