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The effects of 24-epibrassinolide (EBR) on chlorophyll fluorescence, leaf surface morphology and cellular ultrastructure of grape seedlings (Vitis vinifera L.) under water stress were investigated. The grape seedlings were subjected to 10 % (w/v) polyethylene glycol (PEG-6000) and treated with 0.05, 0.10 or 0.20 mg L⁻¹ EBR, respectively. EBR application increased chlorophyll contents, the effective photochemical quantum yield of PSII, maximum photochemical efficiency of PSII, maximal fluorescence and non-photochemical quenching coefficient under water stress in each concentration. Compared with water stress control, higher stomatal density and stomatal length were observed in young leaves under EBR treatments, but not in mature leaves. In-depth analysis of the ultrastructure of leaves indicated that water stress induced disappearance of nucleus, chloroplast swelling, fractured mitochondrial cristae and disorder of thylakoid arrangement both in young leaves and mature leaves. However, EBR application counteracted the detrimental effects of water stress on the structure of the photosynthetic apparatus better in young leaves than in mature leaves. Compared to the other treatments, treatment of 0.10 mg L⁻¹ EBR had best ameliorative effect against water stress. These results suggested that exogenous EBR could alleviate water stress-induced inhibition of photosynthesis on grape possibly through increasing chlorophyll content, lessening the stomatal and non-stomatal limitation of photosynthesis performance.
Antibiotics, as special pollutants in piggery wastewater, have negative effects on wastewater treatment processes. However, it is difficult to obtain a clear understanding of the toxic potency of antibiotics using the common inhibitory experiments of wastewater because of their low concentrations. Therefore, this study for the first time reports research on examining the toxic strength of piggery wastewater containing antibiotics by acute toxicity experiments using luminescent bacteria. Also reported herein for the first time were the mixed toxic effects of different antibiotics on luminescent bacteria using orthogonal experimental designs. Results showed that piggery wastewater had high ecotoxicity equal to 24.6 mg∙L⁻¹ HgCl₂, and the toxicity units (TU) of Chlortetracycline, amoxicillin, florfenicol, and sulfamethazine (SM₂) were 85.5, 64.72, 1.27, and 1.20, respectively, which fell under high or significant ecotoxicity according to the Toxicity Classification System (TCS). The IC₁₀ values indicated that the antibiotics could impose single or mixed toxic effects on luminescent bacteria even in trace concentrations. Among these antibiotics, Chlortetracycline was the most significant one needing more attention in the treatment of wastewater-containing antibiotics.
The objective of the study was to evaluate the effects of increasing dietary standardized ileal digestible (SID) lysine (Lys) level on the expression of jejunal amino acids (AAs) transporters and the microflora in the hindgut of weaned pigs. One hundred and twenty weanling pigs weighing 8.10 ± 0.48 kg were randomly assigned according to body weight and sex to 5 treatments with 6 replicates per treatment and 4 pigs per replicate. Pigs were fed diets with 0.98, 1.11, 1.23, 1.35 or 1.48% of SID Lys for 28 days. The mRNA expression of cationic amino acids transporter 1 (CAT1) in jejunum was higher in groups fed with 1.23, 1.35 and 1.48% SID Lys addition (P < 0.05). There was stated a linear increase in the mRNA expressions of CAT1, excitatory amino acids carrier 1 (EAAC1) and peptide transporter T1 (PEPT1) (linear, P < 0.05). In the caecum, the populations of bacteria and the content of butyric acid were significantly influenced (P < 0.05) by dietary SID Lys. In animals fed diet with 1.35% SID Lys a content of butyric acid was the highest (P < 0.05). Moreover, the populations of Lactobacillus and Bifidobacterium in caecum and colon increased (linear, P < 0.05; quadratic, P < 0.05) as dietary SID Lys level increased. The obtained results showed that dietary SID Lys level may influence AAs absorption and promote the hindgut health. The suggested supplementation of SID Lys to the weaned pigs’ diet is 1.35%.
The objective of this study was to evaluate the effect of different fiber sources on jejunal digestive and absorptive physiology in weaned piglets. One hundred weaned piglets were allotted according to body weight, gender and litter to four dietary treatments. Each treatment was replicated in 5 pens of 5 pigs each. The experimental diets contained 10% of a fiber source: wheat bran (WB), maize fiber (MF), soyabean fiber (SF), or pea fiber (PF). Piglets were fed ad libitum for 30 d. Reduced villus height and villus height-to-crypt depth ratio (P < 0.05) were observed in pigs fed diet MF compared with pigs fed diet WB. In the mid-jejunal mucosa, lower activities of sucrase (P < 0.05) were found in pigs fed diets MF and SF compared with WB. In mid-jejunal digesta, inclusion of SF and PF decreased the activities of trypsin and lipase (P < 0.05) compared with inclusion of WB. Supplementation with PF resulted in higher facilitated glucose transporter 2 (P < 0.05) and lower excitatory amino acid carrier 1 mRNA levels compared with supplementing WB. The apparent faecal digestibility of gross energy, dry matter, organic matter, neutral detergent fiber and acid detergent fiber declined in the following order: diet SF > PF > MF > WB. Our study indicates that SF and PF could promote apparent faecal digestibility in piglets, but this is not accompanied by better jejunal morphology or digestive enzyme activities as compared with WB.
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