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The plants of mung bean (Vigna radiata L. Wilczek) were raised from the seeds soaked in water (control), IAA or 4-C-IAA (10⁻⁶, 10⁻⁸ or 10⁻¹⁰ M) for 8 or 12 h. The plants were allowed to grow in a net house and were sampled at 30 and 45 days after sowing (DAS). Both IAA and 4-Cl-IAA significantly affected the growth (length, fresh and dry mass of roots and shoots), the number of nodules, their fresh and dry mass and the activity of nitrogenase. However, the contents of nitrogen and carbohydrate exhibited a decrease in response to both the auxins. 4-Cl-IAA, at a concentration of 10⁻⁸ M, generated the best response. Moreover, 4-Cl-IAA at other two concentrations (10⁻⁶ and 10⁻¹⁰ M) was much more active than any of the IAA concentration used.
Lead (Pb) is a toxic heavy metal that causes physiological and morphological changes in plants. Our current study was aimed at observing Pb-induced changes in seeds of two upland cotton varieties (TM-1 and Z- 747) that were exposed for 24 hours to various Pb concentrations (0, 50, 100, 300, and 500 μM). Seeds of TM-1 showed higher germination percentages, water uptake, and cell viability as compared to Z-747. Changes in nuclei shape, their partial disappearance, and increase in vacuole size were the initial signs of lead toxicity in Z-747, while TM-1 maintained its typical conformations. Cell wall thickening; increase in the number of mitochondria, protein, and lipid bodies; and vacuolation were more prominent in TM-1. In contrast, plasmolytic shrinkage and scattered cell organelles were the key characteristics of Z-747 at higher doses of Pb. Those observations demonstrated that seeds of TM-1 were more tolerant to Pb stress as compared to Z-747 and may be considered a preferred choice for culture on Pb-contaminated soils.
Wheat, a glycophyte grown in tropical and subtropical regions, is frequently being subjected to soil salinity ultimately affecting the plant growth and yield. Focus of the present study was to evaluate the ameliorative efficiency of different seed priming methods including hydropriming and halopriming [KCl and CaCl2 (100 mM)] by observing change in the expression of antioxidant defense system and accumulation of phenolic as well as proline in the spring wheat Lu26s (salt tolerant) and Lasani-06 (salt sensitive), grown under salt stress of 100 mM NaCl. Results showed that salt stress provoked a marked decline in germination, growth and yield parameters as well as increased lipid peroxidation and hydrogen peroxide (H2O2) contents. However, higher accumulation of proline and low H2O2 contents were recorded in both cultivars under halopriming followed by hydropriming. Halopriming induced a significant increase in antioxidant enzyme activities (CAT, POD, APX) of salt-tolerant cultivar Lu26s, whereas such pattern of enhanced activities of antioxidant enzymes in cultivar Lasani-06 was also found but the content of these activities was less than control under saline regime. The cultivar Lu26s (salt tolerant) maintained lower Na+ and higher K+/Na+ ratio in leaves than salt-sensitive cultivar Lasani-06. Reason behind the loss of grain yield under salinity was found due to the reduction in the grain spike-1 in cultivar Lasani-06, while, in cultivar Lu26s, it was due to decrease in the size of grains. Enhanced germination, low proline and Na+ contents stimulated antioxidant activities as well as phenolic contents associated with improved salt tolerance in haloprimed plants. These results suggest that halopriming is an efficient approach for imparting tolerance in wheat against salinity stress.
To investigate the effect of exogenously applied 28-homobrassinolide (HBL) on drought-stressed plants, photosynthesis and antioxidant systems were examined in Indian mustard (Brassica juncea L.). Seedlings of Indian mustard were subjected to drought stress for 7 days at the 8–14 (DS1)/15–21 (DS2) days’ stage of growth and then returned to normal conditions of growth. These seedlings were sprayed with HBL (0.01 µM) at the 30-day stage and were sampled at 60 days to assess the changes in growth, photosynthesis and antioxidant enzymes. Plants exposed to stress at either of the stages of growth exhibited a significant decrease in growth and photosynthesis. The exposure of plants to stress at an earlier stage (DS1) was more inhibitory than that at a later stage (DS2). However, the follow-up treatment with HBL significantly improved the values of these parameters and also overcame the inhibitory effect of water stress. The activity of antioxidant enzymes [catalase (E.C. 1.11.1.6), peroxidase (E.C. 1.11.1.7) and superoxide dismutase (E.C. 1.15.1.1)] and proline content in leaves exhibited an increase in response to both the treatment factors, where their interaction had an additive effect. It was, therefore, concluded that the elevated antioxidant system, at least in part, was responsible for amelioration of the drought stress.
The complexes of Cu and Mn metals with carboxylates (pivalic acid and benzoic acid) and imidazole were prepared and characterized. The synthesized complexes were screened for antimicrobial activity and cytotoxicity. The FTIR technique was used for characterization. The antibacterial assay revealed that the ligand and complexes are active against Escherichia coli, Staphylococcus aureus and Pasteurella multocida bacterial strains. The haemolytic assay revealed that [Cu(imi)(N₃)] and [MnIII(piv)(imi)(N₃)] were nontoxic, whereas [Cu(piv)(imi)] and [MnIII(Benz)(imi)(OCH₃)] showed moderate toxicity, and [Mn(Him)(N₃)₂] was highly toxic against human red blood cells (RBCs). Results revealed that Cu and Mn complexes with carboxylates (pivalic acid and benzoic acid) have antimicrobial activities. However, haemlytic assay revealed that the metal complex cytotoxicity was variable and biological activity evaluatuion of newly synthesized metals complexes is suggested for safe applications.
Propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy) benzylamino)benzoate (ZJ0273) is a new herbicide which inhibits acetolactate synthase (ALS). The ZJ0273 is considered as safe for the environment and exhibits a satisfactory effect on weed control in the rapeseed field. ALS is the key enzyme of reactions in the biosynthesis of total amino acids (TAAs) especially branched-chain amino acids (BCAAs). This study reports the effect of ZJ0273 on BCAAs and TAAs in rapeseed leaves using near-infrared spectroscopy (NIRS) techniques. A decrease in TAAs and BCAAs contents was observed as the herbicide dosages were increased along with leaf senescence. The wavelengths 2,416 and 1,340 nm were selected to develop the NIRS model for detecting BCAAs and TAAs, and correlation coefficients of model’s prediction set were 0.9823, 0.9764, 0.9831, and 0.9968 for valine, isoleucine, leucine, and TAAs, respectively. The results indicated that 100 mg/L ZJ0273 was a safe dosage for oilseed rape as it did not show a significant effect on the contents of amino acids compared to other higher dosages (500 and 1,000 mg/L).
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