Wyniki wyszukiwania

1

Determination of the absolute number of transgene copies in CMVFut transgenic pigs

100%

Lipinski D., Zeyland J., Plawski A., Slomski R.

Annals of Animal Science

|

2012

|

tom 12

|

nr 3

2

Produkcja bialek terapeutycznych w nasieniu transgenicznych zwierzat

100%

Lipinski D., Juzwa W., Zeyland J., Slomski R.

Biotechnologia

|

2006

|

nr 1

44-52

3

Immune modulation in xenotransplantation

100%

Boksa M., Zeyland J., Slomski R., Lipinski D.

Archivum Immunologiae et Therapiae Experimentalis

|

2015

|

tom 63

|

nr 3

4

The current state of xenotransplantation

100%

Zeyland J., Lipinski D., Slomski R.

Journal of Applied Genetics

|

2015

|

tom 56

|

nr 2

5

Produkcja biofarmaceutykow z wykorzystaniem transgenicznych zwierzat

100%

Lipinski D., Zeyland J., Slomski R.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 03

261-265

Two discoveries reported in the mid 1970s in hybridoma technology and genetic engineering techniques - have enabled overcoming the difficulties related to the generation of pharmaceuticals from their natural resources, such as blood or human tissues, and in 1982 led to the introduction of the first product of the modern biotechnology, a recombinant insulin, opening the biopharmaceutical era. Among the registered biopharmaceuticals in the 1980s and the early 1990s the majority of the products were identical to the natural forms of the proteins and their amino acid sequences. Currently, the rapid growth of the contribution of the second generation biopharmaceuticals with genetically introduced modifications is observed. All of the biopharmaceuticals registered on the market until 2005 were generated with the application of E. coli bacteria, yeast, or animal cell lines (CHO, BHK). With the possible registration on the market of the recombinant antithrombin, in 2006 the first therapeutic protein produced in milk of the transgenic goats will appear as a new alternative, cheaper and more effective system enabling the production of complex proteins. The biopharmaceuticals can be generated in blood, milk, urine, semen, egg white of the transgenic animals, and the silk glands of silkworms. At present, several dozens of the protein products which are produced in milk of almost all species of farm animals and in the egg white of the hens are subjected to studies at the clinical phase.

6

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Genetyczne modyfikacje wybranych cech zwierzat

100%

Zeyland J., Lipinski D., Slomski R.

Postępy Nauk Rolniczych

|

2006

|

tom 53

|

nr 6

17-30

7

CRISPR/Cas9 immune system as a tool for genome engineering

100%

Hryhorowicz M., Lipinski D., Zeyland J., Slomski R.

Archivum Immunologiae et Therapiae Experimentalis

|

2017

|

tom 65

|

nr 3

8

Human CD46, CD55 and CD59 expression influence on swine transgenic fetal fibroblasts' survivability in the presence of human complement components

100%

Zeyland J., Lipinski D., Slomski R.

Annals of Animal Science

|

2012

|

tom 12

|

nr 4

9

Budowa i zastosowanie wybranych glikozoaminoglikanow

88%

Zeyland J., Lipinski D., Juzwa W., Plawski A., Slomski R.

Medycyna Weterynaryjna

|

2006

|

tom 62

|

nr 02

139-144

Proteoglycans can be found in the extracellular matrix of most tissues. They play the role of receptors, take part in cellular adhesion and also interactions between cells. Proteoglycans consist of proteins with one or more covalently bonded glycosaminoglycans. There are seven different types of glycosaminoglycans: hyaluronic acid, heparin and sulphates of chondroitin, keratan I and II, heparin and dermatan. Hyaluronic acid and chondroitin sulphate appear in synovial fluid where they take part in binding water, preventing mechanical stress and increasing elasticity. Keratan sulphate and chondroitin sulphate are used as potential markers of osteoarthritis. Osteoarthritis is a type of arthritis that is caused by the breakdown and eventual loss of the cartilage of one or more joints. Cartilage is a protein substance that serves as a cushion between the bones of the joints. Dermatan sulphate is responsible for the individual resistance to inflammation and pathogens. Understanding the mechanisms of interactions between glycosaminoglycans and pathogens will help to develop more effective vaccinations. The perfect functioning of glycosaminoglycans depends on the efficient activity of both synthesising and degrading enzymes. Even small dysfunctions cause major diseases and disorders.

10

Novel gene constructs for animal transgenesis

88%

Lipinski D., Zeyland J., Juzwa W., Wolko L., Slomski R.

Annals of Animal Science. Supplement

|

2006

|

nr 1

11

1,3-Propanediol production by Escherichia coli using genes from Citrobacter freundii ATCC 8090

76%

Przystalowska H., Zeyland J., Kosmider A., Szalata M., Slomski R., Lipinski D.

Acta Biochimica Polonica

|

2015

|

tom 62

|

nr 3

12

Gene constructs for xenotransplantation

76%

Lipinski D., Juzwa W., Zeyland J., Plawski A., Wielgus K., Slomski R.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr Supplement 1

13

Obtainment of transgenic porcine fibroblast cell lines for the purpose of xenotransplantation

76%

Hryhorowicz M., Nowak A., Grzeskowiak B., Zeyland J., Slomski R., Lipinski D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

14

Agrobacterium -mediated transformation of tobacco and sorghum with a genetic construct containing genes encoding enzymes in the sucrose synthesis pathway

76%

Marszalek M., Zeyland J., Luwanska A., Wielgus K., Slomski R., Lipinski D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

15

Optymalizacja warunków regeneracji in vitro szałwii lekarskiej

76%

Luwanska A., Wielgus K., Szalata M., Lipinski D., Zeyland J., Slomski R.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2013

|

nr 270

16

Tur powroci do polskich lasow?

76%

Slomski R., Lipinski D., Szalata M., Zeyland J., Wielgus K., Dzieduszycki A., Ryba M.

Brać Łowiecka

|

2007

|

nr 11

40

17

Overexpression of gtfbeta gene fragments from Streptococcus mutans encoding Glu and Cat domains in Escherichia coli

76%

Lipinski D., Zandecka-Dziubak J., Juzwa W., Zeyland J., Mrozikiewicz P. M., Slomski R.

Herba Polonica

|

2004

|

tom 50

|

nr 2

Streptococcus mutans is involved in the initiation and progression of dental caries. Fragments of a gtfB gene from Streptococcus mutans encoding Glu and Cat peptides of approximately 35 and 45 kDa, respectively, were cloned from this organism and sequenced. Recombinant Glu and Cat peptides were overexpressed in Escherichia coli with C-terminal sequence containing additional six histidine residues and enterokinase recognition site. The recombinant peptides were purified from E. coli by metal affinity chromatography. These proteins will be used to prepare efficient vaccine against dental caries.

18

Uzyskiwanie swin wykorzystywanych w ksenotransplantacji

64%

Jura J., Slomski R., Smorag Z., Gajda B., Wieczorek J., Lipinski D., Kalak R., Juzwa W., Zeyland J.

Biotechnologia

|

2006

|

nr 1

151-158

19

Production of ZFN-mediated GGTA1 knock-out pigs by microinjection of gene constructs into pronuclei of zygotes

64%

Lipinski D., Nowak-Terpilowska A., Hryhorowicz M., Jura J., Korcz A., Slomski R., Juzwa W., Mazurkiewicz N., Smorag Z., Zeyland J.

Polish Journal of Veterinary Sciences

|

2019

|

tom 22

|

nr 1

20

Molecular and cytogenetic characterization of human albumin transgenic goat fibroblasts as a source of nuclei in somatic cloning

64%

Wozniak A., Lipinski D., Zeyland J., Nowak A., Nuc K., Rynska B., Smorag Z., Slomski R.

Annals of Animal Science

|

2011

|

tom 11

|

nr 1

Ograniczanie wyników

Determination of the absolute number of transgene copies in CMVFut transgenic pigs

Produkcja bialek terapeutycznych w nasieniu transgenicznych zwierzat

Immune modulation in xenotransplantation

The current state of xenotransplantation

Produkcja biofarmaceutykow z wykorzystaniem transgenicznych zwierzat

Genetyczne modyfikacje wybranych cech zwierzat

CRISPR/Cas9 immune system as a tool for genome engineering

Human CD46, CD55 and CD59 expression influence on swine transgenic fetal fibroblasts' survivability in the presence of human complement components

Budowa i zastosowanie wybranych glikozoaminoglikanow

Novel gene constructs for animal transgenesis

1,3-Propanediol production by Escherichia coli using genes from Citrobacter freundii ATCC 8090

Gene constructs for xenotransplantation

Obtainment of transgenic porcine fibroblast cell lines for the purpose of xenotransplantation

Agrobacterium -mediated transformation of tobacco and sorghum with a genetic construct containing genes encoding enzymes in the sucrose synthesis pathway

Optymalizacja warunków regeneracji in vitro szałwii lekarskiej

Tur powroci do polskich lasow?

Overexpression of gtfbeta gene fragments from Streptococcus mutans encoding Glu and Cat domains in Escherichia coli

Uzyskiwanie swin wykorzystywanych w ksenotransplantacji

Production of ZFN-mediated GGTA1 knock-out pigs by microinjection of gene constructs into pronuclei of zygotes

Molecular and cytogenetic characterization of human albumin transgenic goat fibroblasts as a source of nuclei in somatic cloning