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1
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Obecność zdziczałych kotów domowych jako czynnik zagrażający światowej bioróżnorodności

100%
Kamieniak J., Mazurkiewicz T., Tietze M.
Życie Weterynaryjne
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2016
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tom 91
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nr 02
2
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Modyfikujący wpływ człowieka na zachowanie się psa domowego

100%
Kamieniak J., Mazurkiewicz T., Tietze M.
Życie Weterynaryjne
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2015
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tom 90
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nr 06
3

Zabawa jako podstawowy czynnik dobrostanu kształtujący prawidłowe zachowania psychosomatyczne i socjalne psów

100%
Karpinski M., Mazurkiewicz T., Czyzowski P., Goleman M.
Medycyna Weterynaryjna
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2012
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tom 68
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nr 04
Humans have long tried to understand the essence of play. It is important for a dog’s mental health to have sufficient life variety and contact with other dogs. Pleasure resulting from play produces in dogs a desirable mental balance. The dog belongs to a group of animals whose play-related behavior has an important role in ontogeny. During play, puppies learn social behavior from one another. Later, they use this experience as adult dogs. On the basis of play behavior, one can assess a dog’s mental maturity. By playing, dogs learn how to deal with other dogs, animals and humans. Dogs prefer dyadic play, that is, play between two individuals. A dog’s play behavior is affected by factors such as age, sex and body size. Willingness to play and the way of playing also depends on whether both dogs are on a leash, or only one, or neither. Types of behavior related to play include invitations to play, offensive behavior such as climbing on the other dog or pushing the other dog down to the ground, as well as affiliative behavior. When dogs play with a toy, their play is mostly competitive, consisting in attempts to take control of the object. When a dog’s partner in playing is a man, the dog is generally less willing to win, and a mutual interaction between the dog and the man is more important. Humans can modify dogs’ behavior associated with showing willingness to play by sterilization or by activities such as imposing one’s company. The owners’ ability to recognize and differentiate between offensive and aggressive behavior in puppies may reduce the incidence of dogs’ open aggression in adulthood.
4
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Modyfikacja czynników środowiskowych w schronisku dla zwierząt i jej wpływ na sukces adopcyjny

100%
Karpinski M., Mazurkiewicz T., Czyzowski P.
Życie Weterynaryjne
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2012
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tom 87
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nr 08
5
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Pies jako typowy drapieżnik komunikujący się z człowiekiem

100%
Kamieniak J., Mazurkiewicz T., Tietze M.
Życie Weterynaryjne
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2016
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tom 91
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nr 04
6

Wybrane testy behawioralne stosowane za granicą w schroniskach przy adopcji psów

88%
Karpinski M., Mazurkiewicz T., Drozd L., Goleman M., Czyzowski P.
Medycyna Weterynaryjna
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2013
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tom 69
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nr 07
Man’s connection with the dog has lasted thousands of years. Over that period, humans have influenced the physical characteristics and behavior of Canis familiaris through the process of artificial selection. The role of the domestic dog, however, has been changing along with human civilization, rendering some traits of canine behavior undesirable. The innate inclination of Canis familiaris to display aggression may influence its interaction with humans. Dogs whose behavior diverges from the norms approved by society are often placed in shelters for animals, which take care of homeless animals as well as of pets left by their owners. Consequently, animal shelters have to deal with dogs exhibiting various forms of aggressive behavior. The identification of these behavioral problems is extremely important if the animals are to be offered for adopted. In order to detect abnormal behavior in dogs, animal shelters use a number of methods, the most popular of which are various behavioral tests. The tests, however, have certain drawbacks and are not very effective in discovering behavioral problems in dogs. That is why animal shelters ought to use only reliable tests and discuss all important aspects of the dog’s behavior with its potential new owner. It is necessary to use a comprehensive approach in identifying the risks related to the dog’s aggressive behavior.
7
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Evaluation of prognostic parameters of E-cadherin status in breast cancer treatment

88%
Brzozowska A., Sodolski T., Duma D., Mazurkiewicz T., Mazurkiewicz M.
Annals of Agricultural and Environmental Medicine
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2012
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tom 19
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nr 3
Introduction and objective. Breast cancer is one of the most frequent malignancies in women. Axillary lymph node involvement, tumour size, receptor status, and level of malignancy are the most significant prognostic factors in breast cancer, but insufficient to date. More factors are needed for establishing the prognosis and treatment in these patients. The aim of the presented study was evaluation of E- cadherin expression and its prognostic value among 89 specimens of breast cancer. Materials and methods. 89 formalin-fixed and paraffin-embedded breast cancer specimens were studied for expression of E-cadherin detected by immunohistochemistry. During 10-year observation overall/OS/and disease-free survival/DFS/ of patients were assessed. Results. Average of OS and DFS were shorter among patients without expression of E-cadherin in comparison to survival time of patients with expression of E-cadherin. The lack of E-cadherin expression was present more often among patients with distant metastasis. No essential changes were noticed in the level of E-cadherin depending on the size of the tumour, G, presence of metastasis into the lymph nodes, ER, PR and HER-2, hormonal condition and presence of cancerous tissues in lymphatic vessels and the infiltration of lymph nodes capsules.
8

The presence of pamidronate in bone cement affects serum biochemical markers in the rat

76%
Matuszewski L., Matuszewska A., Jaszek M., Stefaniuk D., Grzywnowicz K., Mazurkiewicz T., Polkowska I.
Bulletin of the Veterinary Institute in Puławy
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2014
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tom 58
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nr 2
The main aim of the study was to assess whether the presence of biphosphate Pamidronate (PA) in the cement implanted into the tibial bones had any effect on the chosen biochemical markers in rat's serum characterising homeostasis. Forty adult male Wistar rats were divided into two control groups and two experimental groups. Tibial bone of rats in the experimental groups was implanted with PA-enriched cement, whereas the bone in control-group's rats was implanted with cement without PA. Serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatine kinase (CK) were determined three and six weeks after the surgery. Statistically significant differences in the activities of AST and CK of the rats after implantation with non-enriched cement when compared to rats given PA-enriched cement implantation, were tound. Six weeks after treatment, AST levels decreased significantly in rats with PA-enriched cement, whereas rats in the control group (implanted with non-enriched cement) demonstrated a significant increase in AST activity in comparison to the same values determined after three weeks and values of PA-enriched cement rats determined after six weeks. The activities of CK were higher in rats with PA-enriched implants than in the control group three weeks after surgery, but six weeks after the treatment, rats implanted with enriched cement reached lower values than animals implanted with non-enriched cement. The use of PA in the cement had also some positive effect on the homeostasis of the rats after the surgery and a positive influence on the post operative muscle regeneration process.
9

Changes in physical properties of the bisphosphonate-enriched bone cement

76%
Olchowik G., Matuszewski L., Zdrojewska A., Mazurkiewicz T., Gospodarek M., Kowalczyk B.
Current Topics in Biophysics. Supplement
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2013
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tom 36
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nr 2A: Posters
10

Determination of pamidronate in bisphosphonate-enriched bone cement by ion-pair hplc and capillary electrophoresis

76%
Matuszewski L., Matuszewska A., Mazurkiewicz T., Polkowska I., Jaszek M., Graz M., Gagala J.
Bulletin of the Veterinary Institute in Puławy
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2013
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tom 57
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nr 2
The presence of pamidronate during local use of bisphosphonates (BP)-enriched bone cement was determined. The question was whether pamidronate implanted into the bone cement is eluted. The study was performed on 10 probes of BP-enriched bone cement located in 0.9% NaCl. The probes were incubated for 3 and 6 weeks. Ion-pair HPLC was used for the detection of pamidronate. Then, capillary electrophoresis was applied for quantitative analysis of pamidronate in the 3rd and 6th week after incubation. The presence of pamidronate, eluted from BP-enriched bone cement into 0.9% NaCl solution 3 and 6 weeks after incubation, was demonstrated. These results may explain the changes in the level of cytokine RANKL and bone turnover marker osteoprotegrin in rats' serum treated with BP-enriched bone cement 3 and 6 weeks after surgery. The possibility of effective local use of BP-enriched bone cement in veterinary medicine was underlined. The results, and the former conducted research, point out that the clinical applications of BP-enriched bone cement in vivo may have some validity in the future.
11

Investigation of the actual causes of hip joint implant loosening classified as aseptic – analysis of microbiological culture results and levels of inflammatory markers

76%
Strzelec-Nowak D., Koziol-Montewka M., Niedzwiadek J., Bogut A., Blacha J., Mazurkiewicz T.
Polish Journal of Microbiology
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2015
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tom 64
|
nr 2
Loosening of the hip joint prosthesis is considered as one of the most significant postoperative complications in recent years. The laboratory diagnostic procedure used to differentiate periprosthetic infection from aseptic loosening is very difficult because of the biofilm which microorganisms form on the implant surface. The purpose of this research was to evaluate the level of concordance between clinical classification of implant loosening among 50 patients subjected to reimplantation procedure and laboratory investigation of PJI including microbiological culture results and the levels of inflammatory markers assessed in the patients’ synovial fluid samples, serum, and full blood. The synovial fluid was collected for leukocyte count, differential cell count, and culture on standard media. The levels of systemic inflammation markers such as the ESR and CRP concentration were determined in serum and full blood. Tissue samples were collected for microbiological studies. Components from endoprostheses were exposed to ultrasound in a process called sonication. Among the parameters measured in serum and full blood the levels of ESR and CRP were higher in the septic group of patients. Cytologic analysis of synovial fluid was in correlation with microbiologic identification. The most frequent isolated bacteria was Staphylococcus epidermidis. Culture results from materials such as synovial fluid, sonicate and tissues are crucial to establish the infectious aetiology of the loosening. Microscopic analysis of synovial fluid represents a simple, rapid and accurate method for differentiating PJI from aseptic failure. Sonication increases detection of the infectious process, and culture results are in correlation with the cytologic analysis of synovial fluid.
12
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Characterization of Staphylococcus epidermidis isolates cultured from patients with infected hip prostheses

76%
Bogut A., Niedzwiadek J., Strzelec-Nowak D., Plewik D., Mazurkiewicz T., Marczynski W., Olender A.
Health Problems of Civilization
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2018
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tom 12
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nr 2
Background. Coagulase negative staphylococci are at the forefront of etiologic agents of periprosthetic joint infections (PJIs). The purpose of the study was to characterise causative isolates (n=19) of Staphylococcus epidermidis (SE) – with emphasis on their phenotypic and genotypic heterogeneity. Material and methods. The isolates were cultured from multiple samples obtained perioperatively during revision surgery from 14 patients with clinically and/or microbiologically proven PJI. Phenotypic heterogeneity included variations of colony morphologies, drug resistance patterns and/or the capability of the biofilm formation and was verified by the DNA fingerprinting assay. Results. Phenotypic discrepancies were observed between isolates cultured from 5 patients (35.7%). The genotyping assay identified 3 pairs of isolates as unrelated; single pairs were genetically related and indistinguishable. The biofilm production was detected in 17 isolates, among which 5 (29.4%) were proficient biofilm formers harbouring the icaADBC genes. Additionally, one ica-positive isolate produced a moderate, protease-sensitive biofilm. The remaining isolates were moderate biofilm producers among which four developed protease-sensitive biofilms. Conclusions. The majority of PJIs are monoclonal; nevertheless, phenotypic diversity of SE is a frequent phenomenon which can complicate the diagnostic proceeding. Adherence ability is an important pathogenic trait of SE although the chemical composition of the resultant biofilm, its intensity and regulation of development can vary.
13

Sonication as a diagnostic approach used to investigate the infectious etiology of prosthetic hip joint loosening

64%
Bogut A., Niedzwiadek J., Koziol-Montewka M., Strzelec-Nowak D., Blacha J., Mazurkiewicz T., Macias J., Marczynski W.
Polish Journal of Microbiology
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2014
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tom 63
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nr 3
The purpose of the study was to evaluate the usefulness of sonication for the diagnosis of prosthetic joint infections (PJIs) by its comparison with periprosthetic tissues (PTs) and synovial fluid (SV-F) cultures. The study groups included 54 patients undergoing exchange of total hip prostheses for so called “aseptic” loosening occurring without clinical manifestations of an accompanying PJI and 22 patients who developed a sinus tract communicating with the prosthesis which was indicative of an ongoing infectious process. Significant positive culture results were obtained among 10 (18.5%) patients with “aseptic” implant failure and in 18 (81.8%) patients who developed a sinus tract. Sonicate-fluid (S-F) yielded bacterial growth in all culture-positive patients with “aseptic” loosening vs. 15 patients with presumed PJIs. There was a concordance in terms of bacterial species isolated from S-F and conventional cultures from individual patients. Coagulase-negative staphylococci were isolated most frequently. Sensitivity of sonication (75%) exceeded that estimated for PTs (69%) and SV-F (45%) cultures. We conclude that identification of causative agents of PJIs which is critical to further therapeutic decisions is aided by the combination of sonication and conventional culture.
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