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1

Plant regeneration system in protoplast culture of Gentiana genus

100%
Fiuk A., Rybczynski J. J.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
2

Wykorzystanie metod wspolczesnej analizy materialu roslinnego w badaniach somatycznej embriogenezy Gentiana kurroo [Royle]

100%
Fiuk A., Rybczynski J.
Biotechnologia
|
2006
|
nr 4
95-106
3

Możliwości wykorzystania znaczników molekularnych w hodowli zbóż o zwiększonej tolerancyjności na toksyczne działanie jonów glinu

100%
Fiuk A., Aniol A.
Postępy Nauk Rolniczych
|
2010
|
tom 62
|
nr 3
4

Morphogenic capability of Gentiana kurroo Royale seedling and leaf explants

100%
Fiuk A., Rybczynski J. J.
Acta Physiologiae Plantarum
|
2008
|
tom 30
|
nr 2
Experiments have been carried out on seedling and primary leaf explants of Gentiana kurroo Royle. Morphogenic capacities of cotyledons, hypocotyls and roots were investigated using MS (1962) medium supplemented with 4.64 μM kinetin and 2.26, 4.52 or 9.04 μM 2,4-D. Percentage of callusing explants for each combination was inversely proportional to numbers of obtained embryos. Cotyledons showed the highest morphogenic capabilities. To assess the morphogenic potential of leaf explants, 189 combinations of auxin (NAA, dicamba and 2,4-D) and cytokinin (kinetin, BAP, zeatin, CPPU and TDZ) in different concentrations were tested. The presence of NAA with BAP and dicamba with zeatin produced the greatest number of differentiated somatic embryos. Microscopic analysis of responsive explants led to identifying rhizogenic centers, non-embryogenic and embryogenic cells. The best embryo conversion into germlings was obtained on MS medium containing 4.46 μM kinetin, 1.44 μM GA3 and 2.68 μM NAA or ½ MS. Both media were supplemented with 4.0% sucrose and 8.0% agar. Depending on explant origin and conversion medium, 55.8–71.0% of somatic embryos developed into germlings and plants.
5

Gentiana heterokaryons production with application of ECM 2001[BTX] pulse generator

100%
Fiuk A., Rybczynski J. J.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
6

Gentiana kurroo (Royle) w kulturach in vitro

81%
Fiuk A., Rajkiewicz M., Rybczynski J. J.
Biotechnologia
|
2003
|
nr 3
7

Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures

81%
Mikula A., Fiuk A., Rybczynski J. J.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2005
|
tom 47
|
nr 1
8

Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures

80%
Mikula A., Fiuk A., Rybczynski J. J.
Acta Biologica Cracoviensia. Series Botanica
|
2005
|
tom 47
|
nr 1
The paper describes structural and ultrastructural changes in primary explants, induction of embryogenesis, somatic embryo development, and four protocols for cryopreservation of cell suspensions. The changes during tissue culture of hypocotyl and cotyledon explants from 10-day-old seedlings and fragments of leaf explant of Gentiana cruciata L. were studied. Seedling explants were cultured on MS medium supplemented with 1.0 mg/l dicamba + 0.1 mg/l NAA + 2.00 mg/l BAP + 80.0 mg/l adenine sulphate. The hypocotyl callus tissue was initiated by cell divisions of the vascular cylinder, but in cotyledons only parenchyma cells took part in callus formation. The leaf blade expiants usually responded only by proliferation of the wounded surface. The effect of auxins (2,4-D, NAA, DIC) and cytokinins (kinetin, zeatin, BAP) in various concentration and combinations on leaf explant response was examined. Generally, embryos were formed sporadically on media containing NAA (1.64% responding explants) or 2,4-D (0.38%), but were not produced in the presence of dicamba. Production of somatic embryos was more effective from suspension culture than from agar medium. Liquid culture made it possible to maintain the cell suspension’s embryogenic competence for 5 years. For preservation of proembryogenic masses, four protocols of cryopreservation were studied: direct cooling, sorbitol/DMSO treatment, vitrification, and encapsulation. Direct cooling and sorbitol/DMSO treatment was unsuccessful. Vitrified tissue required a minimum 3 weeks of culture on solid medium for cell proliferation to reach the proper fresh weight for manipulation. Alginate beads with PEMs were transferred directly to liquid medium for post-freezing culture. Vitrification and encapsulation maintained high viability of post-freezing PEM, but encapsulation ensured faster restoration of G. cruciata cell suspension.
9

Protoplast culture of Gentiana kurroo Royle

80%
Fiuk A., Rajkiewicz M., Piatkowska M., Rybczynski J. J.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
10
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In vitro morphogenic events in culture of Lotus corniculatus L. seedling root explants

71%
Rybczynski J. J., Karolkowska M., Kaczmarek Z., Mikula A., Fiuk A.
Acta Societatis Botanicorum Poloniae
|
2006
|
tom 75
|
nr 3
191-200
The experiments were carried out on Lotus corniculatus (L.) seedling root explants of the cultivar varieties Skrzeszowicka, Caroll A10 and strain 175. Callus formation and shoot regeneration were the major explant response depended mainly on of the studied genotype and used plant growth regulators (PGRs). Primary cortex of proximal and distal end of explant was the most active tissue for callus proliferation. For shoot primordia differentiation deeper zones of cortex took a part. The process of meristematic centre initiation was not uniform and various level of shoot differentiation events were observed not earlier than 3 weeks of culture. Usually, the shoot primordia regeneration began on proximal rather than distal end of the explant. BAP rather than urea derivatives stimulated shoot proliferation in extended cultures. Increasing of BAP and TDZ concentrations brought about the explant polarity and expansion of the meristematic zones. The explant position in root did not have significant influence on the number of regenerated shoots. The cultures only had better bud formation by TDZ when compared to BAP. BAP stimulated bud formation and development of the shoots from them. Short term of TDZ treatment of explants stimulated meristem formation which developed into buds and shoots. CPPU stimulated callus proliferation and bud formation when explants pretreatment was prolonged from 12 to 36 hrs.
11

TTC test functionality in evaluation of surviving of Gentiana pem after cryopreervation

71%
Mikula A., Niedzielski M., Fiuk A., Zielinska M., Rybczynski J. J.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
12

Photosynthetic activity of in vitro cultured Gentiana kurroo on medium with different sucrose concentrations

61%
Rybczynski J. J., Fiuk A., Borkowska B., Gawronska H., Bernat W., Mikula A.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
13

Effect of sucrose concentration on photosynthetic activity of in vitro cultures Gentiana kurroo (Royle) germlings

61%
Rybczynski J. J., Borkowska B., Fiuk A., Gawronska H., Sliwinska E., Mikula A.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 5
In this work, the effect of sucrose on photosynthetic activity during in vitro culture was studied. Experiments were carried out using uniform somatic embryo-derived germlings of Gentiana kurroo (Royle) confirmed by chromosome counting and flow cytometry technique. Photosynthetic activity was measured by chlorophyll a fluorescence and gas exchange method. The efficiency of photosynthetic apparatus as measured by the ratio Fv/Fm, Yield and qP (light phase of photosynthesis) was the highest when the medium was supplemented with 0.3% sucrose which well corresponded with plant gas exchange. Taking all data into consideration for the best development of photosynthetic apparatus and the most efficient of net photosynthesis of studied germlings would be medium supplemented with 0.2–0.4% of sucrose.
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