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S. occidentalis can be used in pharmacology and vegetation restoration. A pot experiment was conducted to study the effects of biochar and mycorrhizal fungus inoculation on plant growth and heavy metal accumulation. The mycorrhizal infection rate was increased by apple branch biochar but decreased by coconut husk iron-modified biochar. Conversely, soil pH was not affected by mycorrhizal inoculation but was increased by biochar. Compared to the uninoculated control, the combination of apple branch biochar and mycorrhizal inoculation significantly increased the growth of stems, leaves and roots by 226.46%, 163.15% and 86.00%, respectively. The application of apple branch biochar increased the root Pb content, while root Cd, Cr, Cu and Fe were decreased by 36.30%, 13.63%, 3.09% and 7.66%, respectively. Furthermore, the content of all elements in the stems and leaves also decreased. The application of iron-modified biochar alone increased the content of all the elements in the roots by 4.23-109.33%. But their contents in stems and leaves were decreased by iron-modified biochar and mycorrhizal inoculation alone. The combination of biochar and mycorrhizal inoculation most effectively promoted plant growth, enhanced heavy metal uptake by the roots and produced a barrier effect that reduced the transfer of heavy metals from the roots to the shoots. This might constitute a feasible means of promoting the safe utilization of S. occidentalis in phytoremediation.
The Mexican free-tailed bat (Tadarida brasiliensis) is one of the most widely distributed bat species in the Western Hemisphere. Despite their prevalence in urban environments, limited research has been conducted to determine the features of buildings or of the surroundings that might affect the likelihood of a building being selected by Mexican free-tailed bats as a roost. Our study objectives were to improve the current understanding of Mexican free-tailed bat's urban roosting preferences with regard to both microhabitat and macrohabitat. Between August 2010 and August 2012, we conducted acoustic surveys and emergence observations and examined 218 buildings in Waco, TX, USA. A total of 54 day-roosts for Mexican free-tailed bats was identified. At the microhabitat scale, modeling of building characteristics and opening characteristics showed that bats preferred to roost in tall and abandoned buildings. Roost exits were more likely the results of structural damage to buildings and less likely to have vegetation blocking the adjacent air space. Roost accessibility seemed to be more important than thermal condition in roost selection. At the broader macrohabitat scale, bats were more likely to roost in areas with lower income and were near tall buildings and water sources.
Dissimilatory Fe(III) reduction has a profound infl uence on the global cycling of elements and the decontamination of pollutants, depending on the interaction of various environmental conditions. Hydrogelencapsulated goethite/soil was prepared in this study, and anaerobic incubation was conducted to investigate the biological Fe reduction of different encapsulated aggregates by Shewanella oneidensis MR-1. Results indicated that the release of Fe(II) ion was signifi cant and insignifi cant in R-soil treatments with and without anthraquinone-2,6-disulfonate (AQDS), respectively. The increase in the cross-linker ratio in the hydrogel decreased iron reduction. The Fe(II) concentration followed the order of unencapsulated treatment < 0.3% encapsulated hydrogel treatment < 2% encapsulated hydrogel treatment with AQDS addition. The results of the goethite experiment suggested that the goethite level and the addition of AQDS changed the effect of mineral structure property on iron reduction. This result was consistent with the simulation of a reductive dissolution kinetic model, in which the initial iron reduction rate k and long-term Fe(II) ion release extent parameter log γ were controlled by the interaction of the mineral structure property, iron mineral content, and electron shuttle compound distribution. Thus, site-specific environmental conditions should be fully considered in monitoring the performance and environmental effects of biological iron reduction.
In order to monitor and correct the meteorological factors of the spring gravity meter, the characteristics of the time varying gravity changes caused by meteorological factors were analyzed. Kunming GS15 gravity meter from 2007 continuous gravity observation has been carried out with the sampling rate of the pressure observation. In this study, we first compare the effects of 4 types of gravity meter and 3 different types of stations on the gravity observed seasonal gravity signals. It is indicated that the observed seasonal gravity signal of the cave is only 1/10, and there is a constant temperature and constant pressure device. Compared with the same time, the gravity signal of the gravity signal is about 100 times smaller. The influence of the pressure load of the gravity meter is tested by using the theory of pressure. The results show that only the actual value of 2cpd - 3cpd pressure load varies from -0.395 to -0.280×10-8ms-2 , and the 1cpd to 1 cpm periodic partial type gravity meter is also in accordance with the law of gravity and air pressure. And with the characteristics of time lag. In this paper, the nonlinear zero drift parameters of the linear regression model with time lag and the time series of the GS15 gravity meter are used to simulate the nonlinear zero drift parameters of the gravity meter. The results show that the gravity signal contains time lag 35 hours, and the air pressure admittance is 0.8 × 10-8ms-2/mbar. The correlation can reach 79%. The gravity changes signal and satellite gravity as well as the gravity water load signal of the land water model are the same as the gravity water load signal in the autumn as the minimum value, and the seasonal variation of the maximum value of gravity in summer
Based on 2005-15 water quality data and measured flood data in 2006-12 at Tongguan Bridge Section, storm-period water-quality characteristics and different time-scale water-quality characteristics have been analyzed for the Weihe River and its basins. The interannual and quarterly mean concentrations of COD, NH₃-N, DO, and river pH value are calculated to evaluate pollution characteristics. Water quality in flood and non-flood periods were monitored to explore the relationship between peak flows and water quality in stormwater. The results showed that in the stormwater period, index concentrations and their fluctuation ranges decreased and were reduced gradually with the increase of peak flow. When peak flow was in the range of 480-680m³/s, concentrations of COD and NH₃-N had the same trend of increasing and decreasing in general. During 2005-15, river pH value fluctuated in the range of 7 to 8, and pH value in the second quarter was slightly higher than in the other three quarters. Water quality of the Weihe improved significantly after 2010.
A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl2, and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.
Untranslated regions (UTRs) of eukaryotic mRNAs plav crucial roles in post-transcriptional regulation of gene expression via the modulation of nucleocytoplasmic mRNA transport, translation efficiency, subcellular localization, and message stability. Single-nucleotide polymorphisms (SNPs) in UTRs of a candidate gene may also change the post-transcriptional regulation of a gene or function by nucleotide mutation. For species that have not been entirely sequenced genomically, new methods need to be devised to discover SNPs in noncoding regions of candidate genes. In this study, based on the expressed sequence tag (EST) of Pinus radiata (Monterey pine), we obtained all the sequences of UTRs of the actin gene by using a chromosome walking method. We also detected all the SNPs in and around the coding region of the actin gene. In this way, the full genomie sequence (2154 bp) of the actin gene was identified, including the 5'UTR, introns, the coding sequence, and the 3'UTR. PCR amplification and DNA fragment sequencing from 200 unrelated P. radiata trees revealed a total of 21 SNPs in the actin gene, of which 3 were located in the 5'UTR, 3 in the introns, 10 in the coding sequence, and 5 in the 3'UTR. We show that chromosome walking can be used for obtaining the sequence of UTRs, and then, based on this sequence, to discover SNPs in the noncoding regions of candidate genes from this species without an entire genomic sequence.
The net photosynthetic rate (PN), the sample room CO₂ concentration (CO₂S) and the intercellular CO₂ concentration (Ci) in response to PAR, of C3 (wheat and bean) and C4 (maize and three-colored amaranth) plants were measured. Results showed that photorespiration (Rp) of wheat and bean could not occur at 2 % O₂. At 2 % O₂ and 0 µmol mol-1 CO₂, PN can be used to estimate the rate of mitochondrial respiration in the light (Rd). The Rd decreased with increasing PAR, and ranged between 3.20 and 2.09 µmol CO₂ m⁻² s⁻¹ in wheat. The trend was similar for bean (between 2.95 and 1.70 µmol CO₂ m⁻² s⁻¹), maize (between 2.27 and 0.62 µmol CO₂ m⁻² s⁻¹) and three-colored amaranth (between 1.37 and 0.49 µmol CO₂ m⁻² s⁻¹). The widely observed phenomenon of Rd being lower than Rn can be attributed to refixation, rather than light inhibition. For all plants tested, CO₂ recovery rates increased with increasing light intensity from 32 to 55 % (wheat), 29 to 59 % (bean), 54 to 87 % (maize) and 72 to 90 % (three-colored amaranth) at 50 and 2,000 µmol m⁻² s⁻¹, respectively.
Breeding scientists have given extensive attention to triploids in trees because of their importance to forestry. Consequently, creating and breeding triploids of good quality has become one of purposes of tree breeding. We chose two autotetraploids (Betula platyphylla, named Q10 and Q65) as female parents and eight hybrid diploids (B. platyphylla × B. pendula, named F1 – F8) as male parents to obtain progenies through controllable pollination, resulting in triploid progenies. Germination rate and germination energy of triploid seeds of Q65 were significantly higher (P < 0.01) than in triploid seed s of Q10. Triploid families with Q65 as female parent had a large quantity of saplings, whereas triploid families with Q10 as female parent had a small quantity of saplings. Triploid families with Q65 as female parent were generally superior in base diameter and height to base diameter ratio when compared to a diploid family. Q65×F3 was preliminarily recognized as the superior family. These results demonstrate that the female parent has a major influence on triploid progenies, although the male parent also has a small influence. The results provided a reference to build seed orchards of triploid birch trees, choose tetraploids as female parents and forecast triploid families of good quality.
The structural configuration of bioretention plays an important role in the consumption and purification of nitrogen pollutants in rainfall runoff. Three layered bioretention tanks – 7#, 9#, and 10# – with artificial packing layers of fly ash mixing sand, blast furnace slag, and planting soil, respectively, were selected for intermittent and continuous operational tests. All load-reduction rates of nitrogen pollutants for intermittent running test exceeded 40% in three tanks, and tank 7# showed >70%. Moreover, the effluent pollutant concentration of 7# increased with time, whereas those of 9# and 10# fluctuated and then decreased slowly. The correlation model between TN removal and its influencing factors was established using the partial least regression method. Modeling analysis suggested that the filler type was the most important factor affecting TN removal. TN removal was positively correlated with packing factor and submerged zone height, while it was negatively correlated with antecedent dry time and influent loading. Soil pollutant original content and texture classification were detected before the continuous running test. The percentages of NO₃-N and NH₃-N accumulating in three facilities accounted for a total influent load of approximately 77% (7#), 61% (9#), and 43% (10#) when the exhaustion point was reached, demonstrating the relatively poor performance of planting soil.
P5 ATPases (ATP13A1 through ATP13A5) are found in all eukaryotes. They are currently poorly characterized and have unknown substrate specificity. Recent evidence has linked two P5 ATPases to diseases of the nervous system, suggesting possible importance of these proteins within the nervous system. In this study we determined the relative expression of mouse P5 ATPases in development using quantitative real time PCR. We have shown that ATP13A1 and ATP13A2 were both expressed similarly during development, with the highest expression levels at the peak of neurogenesis. ATP13A3 was expressed highly during organogenesis with one of its isoforms playing a more predominant role during the period of neuronal development. ATP13A5 was expressed most highly in the adult mouse brain. We also assessed the expression of these genes in various regions of the adult mouse brain. ATP13A1 to ATP13A4 were expressed differentially in the cerebral cortex, hippocampus, brainstem and cerebellum while levels of ATP13A5 were fairly constant between these brain regions. Moreover, we demonstrated expression of the ATP13A4 protein in the corresponding brain regions using immunohistochemistry. In summary, this study furthers our knowledge of P5-type ATPases and their potentially important role in the nervous system.
Carbonaceous greenhouse gases (i.e., CO₂, CH₄ ) have often reported to be emitted from the surfaces of reservoirs, while other non-carbonaceous greenhouse gases are not available to many reservoirs. In this study, the spatiotemporal variability of N₂O emissions from Xin’anjiang Reservoir was investigated at the different regions over a 13-month period using the method of the static floating chambers combined with gas chromatography. Results showed that the average N₂O emission flux was the highest in the downstream river (220.24±153.68 μg m⁻²·s⁻¹), followed by the upstream river (49.85±48.55 μg m⁻²·s⁻¹). The reservoir’s main body had the lowest flux (26.61-39.07 μg m⁻²·s⁻¹). Seasonal variability indicated that the average N₂O emission fluxes before the dam reached their maximum in March, which was likely influenced by the disappearance of thermal stratification then, caused a short N₂O flux peak at the water-air interface. A thorough investigation should be carried out at the different reservoir regions for a long-term basis to discover the spatiotemporal variability in N₂O emission flux in a hydroelectric reservoir system, which could avoid the error of estimating the N₂O emissions incorrectly.
The tumblehome hull adopts some novelty designs such as low-tumblehome freeboard and wave-piercing bow. The new form design makes the ship have many special hydrodynamic performances. Especially the green water of tumblehome hull is different from that of hulls with flare free board. Green water is a strong nonlinear phenomenon of ship-wave interaction, the variation of free surface of liquid is complicated, and there are still some difficulties to solve green water problems well with numerical simulation method. In this paper firstly the motion responses of the tumblehome hull was calculated based on 3D potential theory, and then the dam-break flow model was used to calculate green water height and pressure distribution. According to the result of numerical simulation, some typical working conditions are chosen for 3D CFD simulation using RANS method. The results of numerical simulation methods are compared with the experimental results measured in towing tank. The influence of different ship form parameters and wave parameters to the green water of tumblehome hull is analyzed, and some regularities of green water on tumblehome hull in regular waves are summarized
The first appearance of the brachiopod Cyrtospirifer and related forms in the Late Devonian of South China significantly postdates the beginning of the Frasnian and the entry of the group in other parts of the world. Scattered data from different sections suggest that its first entry, associated with the emergence of other plicate spiriferids, such as theodossid and conispiriferid brachiopods, was late in the Middle Frasnian. At the same time, many rhynchonellids disappeared or became extinct locally in South China. This brachiopod faunal overturn near the Palmatolepis punctata–Early Pa. hassi zonal boundary is the most significant event in the Early–Middle Frasnian of South China, characterized by about a 35% loss of existing species and the flourishing of the plicate spiriferids, which was coeval with the end of a major biogeochemical perturbation recently recognized in the Pa. punctata Zone. By contrast, atrypid brachiopods do not seem to show any significant diversity change. The brachiopod faunal change was probably related to a (local?) transgressive event in South China, which also brought new pelagic faunas northwards into some intra−shelf deeper water areas, such as the Shetianqiao area in central Hunan Province. Fifteen brachiopod species are described and illustrated, which include some taxa that are first recorded or recognized in South China, e.g., the spiriferid Pyramidaspirifer, which is now known from both North America and South China. One new species, Desquamatia qiziqiaoensis, is erected.
As a common plant in Momoge Wetland, Scirpus planiculmis is the major food source for Siberian Crane (Grus leucogeranus), which is one of the most valuable waterfowl in Momoge Wetland. Through a simulation experiment, this study investigated the effects of different water table depths (-5, 5, 10, 20, and 30 cm) and salinities (300, 1,000, 3,000, 4,000, and 5,000 mg/L) on the eco-physiological characteristics (height, leaf area per plant, chlorophyll content, and chlorophyll fluorescence) of Scirpus planiculmis. The results indicate that for the seedlings of Scirpus planiculmis, the effects of water depth on plant height, leaf area per plant, and chlorophyll content were significant, while salinity affected height increment, chlorophyll content, and chlorophyll fluorescence parameters. For the plants of Scirpus planiculmis, the water depth had a significant effect on height increment and chlorophyll content, while salinity had a significant effect on height increment, chlorophyll content, and chlorophyll fluorescence parameters. These results indicate a suitable ecological response thresholds of water table and salinity on the growth of Scirpus planiculmis seedlings and plants, which were 5-10 cm and less than 3,000 mg/L for seedlings, 30 cm and 300-4,000 mg/L for plants.
This paper examines the potential value of phosphate-solubilizing bacteria(PSB) in the dissolution of soil phosphorus and in the subsequent immobilization of lead (Pb), both in bacterial growth medium and in soil. In growth medium, Pseudomonas chlororaphis showed both phosphate-solubilizing and Pb-immobilizing capability, the immobilization of Pb was attributed to pyromorphite formation, as indicated by X-ray diffraction analysis. P. chlororaphis cannot multiply in soil in the presence of indigenous soil bacteria; however, when the added content of PSB-immobilized biochar (PIB) was equal to or greater than 800 mg/kg, the PSB could proliferate effective and the NH₄NO₃-extractable Pb concentration was decreased to below 1 mg/kg. Therefore, the inoculation of PIB in soil can be used as an alternative technique to Pb immobilization, thereby avoiding secondary pollution arising from the addition of large amounts of phosphorus as a heavy-metal passivator.
Taking advantage of coastal wave environment to carry out scaled ship model test is an effective testing technology for ship performance. In this paper, the method of spectral analysis is adopted to calculate the significant wave height, period, wave spectrum and some other parameters of some places along the North Sea area of China. The measured wave spectrum and the ocean spectrum are handled into non-dimensional form to evaluate their similarity. The influence of wind direction and tide on coastal waves was analyzed. And the results indicate that the coastal wave spectrum is similar to the ocean spectrum under some specific conditions
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