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  1. 27 Acta Neurobiologiae Experimentalis

  2. 3 Acta Physiologica Polonica

  3. 3 Journal of Physiology and Pharmacology

  4. 1 Acta Biochimica Polonica

  5. 1 Applied Biology Communications. Lublin Scientific Center

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  1. 37 Lewin-Kowalik J.

  2. 18 Larysz-Brysz M.

  3. 11 Gorka D.

  4. 10 Jedrzejowska-Szypulka H.

  5. 8 Golka B.

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  1. 2 2019

  2. 2 2017

  3. 1 2016

  4. 4 2015

  5. 1 2014

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1

Influence of lowered serum calcium level on body temperature and sensibility to thermal stress in rats

100%
Lewin-Kowalik J.
Acta Physiologica Polonica
|
1980
|
tom 31
|
nr 4
2

Czynnik wzrostu nerwu, budowa chemiczna i znaczenie biologiczne

63%
Sieron A., Lewin-Kowalik J.
Postępy Biochemii
|
1988
|
tom 34
|
nr 3
3

Influence of intraperiotonal administration of calcium on body temperature and resistance to thermal stress in rats

63%
Prebendowski J., Lewin-Kowalik J.
Acta Physiologica Polonica
|
1979
|
tom 30
|
nr 1
4

Influence of interaperitoneal administration of calcium on body temperature and resistance to thermal stress in rats

63%
Prebendowski J., Lewin-Kowalik J.
Acta Physiologica Polonica
|
1978
|
tom 29
|
nr 5
5

Modified protocol for culturing predegenerated adult rat Schwann cells

51%
Lewin-Kowalik J., Marcol W., Francuz T., Pietricha-Dutczak M.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
The purpose of this experiment was to optimize the methodology of culturing predegenerated Schwann cells. Right sciatic nerves of adult rats (n=3) were cut and left for 7 days. Then, 1-mm fragments of predegenerated (P) and intact (C) nerves were separately planted in 12-well culture plates precoated with laminin or fibronectin. Medium for culturing of endothelial cells EBM-2 (endothelial cell culture medium) was compared with DMEM (Dulbecco’s Modified Eagle’s Medium). Additionally, culture media were supplemented with factors supporting SCs growth: bovine pituitary extract (5 µg/ml), heregulin (40 ng/ml), and insulin (2.5 ng/ml). After 7 or 14 days, plates were subjected to analysis. Cell culture purity was determined under the fluorescent microscope by estimating the percentage of GFAP, N-Cadherin and NGFR p75-positive cells, and intensity of cell growth - by counting the number of cell islets migrating from nerve explants. Percentage of cells confirmed as Schwann cells was 94–97. Number of islets was significantly higher in both time-frames: (1) in plates precoated with fibronectin in both groups; (2) in P than in C groups. Thus, nerve predegeneration, application of EBM-2 as culture medium and fibronectin as coating appeared a good method for obtaining cultured Schwann cells to be used in different experimental models in rats.
6

Schwann cells induced neuroprotection and regeneration of retinal ganglion cells

51%
Lewin-Kowalik J., Smedowski A., Pietrucha-Dutczak M.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
To investigate neuroprotective effect of intravitreally applied Schwann cells therapy towards Retinal Ganglion Cells (RGCs) in rat experimental glaucoma. Twenty male Wistar rats were included to this study. Experimental glaucoma was induced in the left eye of each rat by intraocular pressure (IOP) elevation using intracameral injection of polystyrene microbeads. The right eye served as a healthy control. Ten animals received intravitreal injection of 5 μl Schwann cells suspension (about 106 cells), another 10 received injection of equivallent volume of PBS. Animals were breaded for 6 weeks and IOP was monitored using laboratory tonometer once a week. After 6 weeks animals were sacrificed, eyes with optic nerves were enucleated and processed for histology and immunohistochemistry. RGCs survival was compared by counting RGCs bodies and optic nerve axons from control eyes (healthy and PBS) and Schwann cells treated. Mean 6 weeks IOP in ocular hypertension eyes was significantly higher in comparison to healthy contralateral eyes (31.02±5.5 mmHg and 10.32±0.54 mmHg, mean±SD, Wilcoxon paired test, P<0.05). There were significant differences between RGCs bodies and optic nerve axons numbers in Schwann cell-treated vs. PBS-treated vs. healthy control eyes (P<0.05, Kruskall-Wallis test). Mean 6-weeks loss of RGCs bodies was 21.7% in glaucoma eyes treated with Schwann cells and 45% in glaucoma eyes treated with PBS. Immunofluorescent staining with GAP43 showed neurites outgrowth within optic nerves from eyes treated with Schwann cells. Applied cellular therapy using predegenerated Schwann cells showed neuroprotective and regenerative effect towards RGCs in rat glaucoma model.
7

Role of IL-1beta in production of TNF-alpha and IL-6 by peripheral mononuclear cells following subarachnoid hemorrhage in rats

51%
Larysz-Brysz M., Jedrzejowska-Szypulka H., Olakowska E., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Subarachnoid hemorrhage (SAH) develops when extravasated arterial blood enters subarachnoid space and mixes with cerebrospinal fluid. This leads to numerous pathologies, including increased synthesis of proinflammatory cytokines, like interleukin-1β (IL-1β). Through broken blood-brain barrier, IL-1β may stimulate peripheral leukocytes. These peripheral mononuclear cells (PMC) may be an additional source of other cytokines and migrating to the brain they can enhance or reduce the pathologies resulting from SAH. We examined the effect of neutralization of IL-1β on secretion of TNF-α and IL-6 by PMCs in adult rats following SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies. Control group consisted of sham-operated rats. Ninety minutes or 24 hrs following surgery, blood samples were collected from the extraorbital plexus and centrifuged to separate leukocyte subpopulations. Isolated PMCs (monocytes and lymphocytes) were cultured for 24 hrs and TNF-α and IL-6 concentrations in the supernatants were assessed with ELISA. SAH led to the increase of production of both TNF-α and IL-6 by PMCs. Neutralization of IL-1β activity significantly reduced the concentration of both cytokines 90 min as well as 24 hrs after SAH. The results indicate an important role of IL-1β in the activation of peripheral mononuclear cells in the course of subarachnoid hemorrhage.
8

Peripheral nerve extracts change their neuroprotective activity upon CNS neurites after NGF blockade

51%
Larysz-Brysz M., Lewin-Kowalik J., Gorka D., Wolwender K.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
9

Minocycline administration protects tight junction proteins from degradation after pre-chiasmatic subarachnoid hemorrhange in rats

51%
Gendosz D., Debska K., Jedrzejewska-Szypulka H., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Subarachnoid hemorrhage has complex, multisystem and multifaceted pathogenesis that involves several ongoing pathological processes, including BBB degradation. Our aim was to survey potential protective properties of minocycline on Tight Junctions (TJ) proteins in rat brain, after experimentally induced pre-chiasmatic SAH (pSAH). METHODS: pSAH was induced by injection of 200 μL of fresh autologous arterial blood into pre-chiasmatic cistern in rat brain. Minocycline was administrated ip twice, at 1st and 10th h after surgery (dose: 30 mg/kg). 24 h following the surgery, animals were perfused transcardialy and whole brains were collected. In order to investigate immuno-localization of TJ proteins, coronal sections were immuno-stained against Zonulin-1, Occludin and Claudin-5. ZO-1, OCN, CLN-5 proteins levels were examined by WB reactions. RESULTS: We observed numerous blood vessels around the site of blood application as well in more distant areas, where we found essential alterations in immunostaining patterns of ZO-1, OCN and CLN-5, comparing to controls. Minocycline administration preserves physiological ZO-1 and OCN cellular localization comparing to SAH group. Subsequently we provided WB reactions to define SAH impact on TJ protein level. We observed that SAH leads to significant decrease in both OCN and ZO-1 protein level in first 24 h after ictus. Important message comes from the minocycline experiment. We found out significant increase of ZO-1 level comparing to SAH group. Though OCN doesn’t reach significance, we can observe some positive trend in minocycline group. CONCLUSIONS: Administration of arterial blood directly to prechiasmatic cistern leads to serious affections of TJ integrity during first 24 h after pSAH. Minocycline protects TJ proteins from degradation and also preserves TJ unit from morphological alterations at the level of brain vessel endothelium.
10

Serum levels of the S100B protein and neuron - specific enolase are associated with mortality in critically ill patients

51%
Duda I., Krzych L., Jedrzejowska-Szypulka H., Lewin-Kowalik J.
Acta Biochimica Polonica
|
2017
|
tom 64
|
nr 4
11

Long term treatment of ketogenic diet increases kidney tumor growth by p53-dependent manner

51%
Liskiewicz A., Kasprowska-Liskiewicz D., Lewin-Kowalik J., Jedrzejowska-Szypulska H.
Acta Neurobiologiae Experimentalis
|
2014
|
tom 74
|
nr 3
The Eker rat is a useful model of tuberous sclerosis, genetic disease with leads to tumor growth in many tissues. Ketogenic diet (KD) is already used in treatment of epilepsy in tuberous sclerosis patients, but its influence on tumor growth is still not clear. Here we reported how KD affects growth of kidney tumors in Eker rats, subjected to the diet for 4, 6 and 8 months. Data were compared to animals fed with standard diet. Obtained results showed that long term treatment of ketogenic diet promotes tumor growth in kidneys. Additionally, biochemical analysis of renal tissue indicate a possible mechanism in growth promoting influence of KD by lowering p53 protein, guardian of the genome.
12

Role of bcl-2 in the optic nerve regeneration induced by peripheral nerve grawts

51%
Wolwender K., Lewin-Kowalik J., Larysz-Brysz M., Fus Z.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
13

Role of IL-1beta in stimulation of peripheral mononuclear cells to secrete ET-1 and in development of vasospasm following subarachnoid hemorrhage in rats

51%
Jedrzejowska-Szypulka H., Larysz-Borysz M., Woszczycka-Korczynska I., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Subarachnoid hemorrhage (SAH) develops when extravasated arterial blood enters subarachnoid space and mixes with cerebrospinal fluid. As a result, much pathology develops, including arterial vasospasm leading to neuronal ischemia resulting in neurological deficits. Cerebral arteries may be constricted both directly by cytokine influence on arterial smooth muscles and indirectly, through increase of endothelin-1 (ET-1) synthesis or adhesion molecules production. Interleukin-1β (IL-1β) is secreted following SAH and through broken bloodbrain barrier it may lead to the stimulation of peripheral leukocytes. These peripheral mononuclear cells (PMC) may be an additional source of ET-1 and migrating to the brain they can enhance the vasospasm. We examined effect of neutralization of IL-1β on secretion of ET-1 by PMCs as well as on basilar artery vasospasm in rats following SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies. Control group consisted of sham-operated rats. Ninety minutes or 24 hrs following surgery, blood samples were collected from the extraorbital plexus and centrifuged to separate leukocyte subpopulations. Isolated PMCs (monocytes and lymphocytes) were cultured for 24 hrs and ET-1 concentration in the supernatants was assessed with ELISA. In brain stem specimens, vasospasm was determined. SAH led to the strong vasospasm and increase of production ET-1 by PMCs. Neutralization of IL-1β activity significantly reduced the ET-1 level in both time-points, but led to decrease of vasospasm only after 24 hrs. The results suggest that ET-1 does not influence the vasospasm in the acute phase but is involved in this process 24 hrs after subarachnoid hemorrhage.
14

Decrease of RGCs number in glaucoma model vs. optic nerve transaction in rats

51%
Pietrucha-Dutczak M., Smedowski A., Wylegala E., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
Introduction: Transection of the optic nerve and glaucoma causes both structural and functional damage to retinal ganglion cells with subsequent vision defect or loss. This study was undertaken to compare the lost of RGCs after optic nerve transection and glaucoma model. Materials and methods: Wistar rats were divided into two groups. The first group underwent bilateral stereotactic injection of fluorescent tracer – Fluorogold (FG) into the superior colliculus to label RGCs. After one week the right optic nerve was transected. Left eye without optic nerve axotomy was established as control. In the second group intraocular pressure (right eye) was elevated by injection of polystyrene microbeads into anterior chamber (Bead model) and measured by Icare TonoLab. RGCs were labeled by FG before euthanasia. Fourteenth days following optic nerve transection and intraocular pressure elevation the total number of FG-positive RGCs was counted in seven radial sections through the optic disk. Results and conclusions: After axotomy the number of surviving cells was reduced to 20.2 % (from 2249.5 ± 127.2 – in control group to 454.7 ± 96.5 – in group after axotomy), in glaucoma model to 79.9% (from 2249.5 ± 127.2 – in control group to 1798.3 ± 118.96 – in glaucoma model).
15

Solid pituitary adenoma in Eker rats subjected to ketogenic diet

51%
Liskiewicz A., Gendosz D., Jedrzejowska-Szypulka H., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
Tuberous sclerosis (TS) is a genetic disease causing non-malignant tumors growth in the brain (e.g. pituitary adenoma) and in other organs. Ketogenic diet is already used in TS patients in treatment of epilepsy. However the mechanism of its influence on tumor growth is still not clear. The Eker rat is a useful model of TS: it has a spontaneous germ line mutation of the TSC2 gene what predisposed them to multiple tumors. In Eker rats, pituitary adenomas are common, occurring in 58% of adults (more than 18-months-old). Methods: Forty six 8-month-old Eker rats (males and females) were used. Twenty six (experimental group) have been maintained on high fat, low carbohydrate ketogenic diet for 6 months, while 20 (control group) received a standard rodent diet. At the age of 14 months rats were sacrificed. Anteroposterior, vertical, and transverse diameters of the found pituitary adenomas were measured. Size of tumors was calculated by using the formula for volume of the ellipsoid. Results: 8% animals from experimental group and 20% from control group have developed pituitary adenomas. Mean tumor volume in experimental group was 143 mm2 vs. 217 mm2 in control animals. Conclusion: Eker rats fed with ketogenic diet develops solid pituitary adenoma in 14 months of age. Incidence of these pituitary tumors in Eker rats fed with ketogenic diet (8%) was lower when compared with rats from control group (20%).
16

IL-1betta stimulates cytokine secretion by peripheral leukocytes following subarachnoid hemorrhage in rats.

45%
Jedrzejowska-Szypulka H., Woszczycka-Korczynska L., Olakowska E., Larysz-Brysz M., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
Much evidence indicates that pathologies observed after subarachnoid hemorrhage (SAH), including vasospasm and neurological defects, are the effects of immuno-infl ammatory processes accompanying the hemorrhage. Following SAH, CSF concentration of cytokines increase, what may change the cerebral perfusion and destroy brain–blood barrier. These cytokines are produced by activated leukocytes as well as hypoxic cells in the brain. The aim of this study was to examine the role of IL-1β in stimulation of peripheral leukocytes to increase secretion of cytokines after SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies (SAH’ groups). Control group consisted of sham-operated rats. Ninety minutes or 24 h following surgery, blood samples were collected and subpopulations of mononuclear leukocytes were isolated and cultured for 24 h. Then, the concentration of TNF-α, ET-1 and IL-6 in supernatants was measured by means of ELISA. SAH resulted in signifi cant increase in TNF-α and ET-1, especially after 24 h, and no change in IL-6 concentration. Inhibition of IL1-β decreased the secretion of TNF-α and ET-1 and led to increase in IL-6. We found that peripheral leukocytes are an additional source of cytokines following SAH, and their activation depends on IL1-β produced after hemorrhage.
17

Positive effects of microcrystalic chitosan application after laminectomy in rats

45%
Larysz-Brysz M., Marcol W., Slusarczyk W., Malinowska-Kolodziej I., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
18

Content and composition of proteins obtained from short-time-predegenerated distal stumps of rat sciatic nerves

45%
Gorka D., Larysz-Brysz M., Lewin-Kowalik J., Krause M., Barski J. J.
Applied Biology Communications. Lublin Scientific Center
|
1994
|
tom 04
|
nr 3-4
19

The effects of peripheral nerve estracts versus BDNF on retinal ganglion cells survival following injury

39%
Golka B., Swiech-Sabuda E., Larysz-Brysz M., Gorka D., Golka D., Marcol W., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
20

Does restruction of jugular venous outflow induce multiple sclerosis-like signs in rat brains?p.S66-S67

39%
Jedrzejowska-Szypulka H., Kotulska K., Kapustka B., Laczynski M., Larysz-Brysz M., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Hypothesis that multiple sclerosis (MS) may be caused by chronic cerebrospinal venous insufficiency (CCVI) has gained public interest from both patients and physicians. However, there’s still lack of evidence for it. We have investigated presence of neuronal demyelination and degeneration, similar to these found in MS, in rat model of CCVI created by occlusion of jugular veins (JVs). METHODS: Twenty-five young female Wistar C rats were used. Complete ligation of both JVs (BO group), left JV (UO), or partial ligation (stenosis resulting in ~70% reduction of blood flow) of both JVs were performed. Blood flow in JVs was measured with Laser Doppler Flow Assessment. Neurological assessment using Neurologic Deficit Scale (NDS), 5-point EAE staging protocol, and gait analysis with CatWalk was performed. After 12 weeks, MRI for detecting demyelinating plaques as well as signs of bloodbrain barrier (BBB) disruption was performed. Histologic analysis of brain specimens was focused on markers of inflammation and demyelination. RESULTS: No neurologic deficits were found in all experimental animals. Both NDS, EAE and gait analysis did not differ from normal. MRI T2- and T1- weighted imaging as well as FLAIR sequence did not reveal any abnormalities in the brains of experimental rats. Histological analysis did not show any signs of inflammation or demyelination. CONCLUSIONS: Twelve-week CCVI in rats, both complete and partial, did not induce any changes resembling pathologies observed in MS. Therefore, linking CCVI with origin of MS remains controversial.
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