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The atricle is devoted to the study of the cultivation of cyanobacteria Microcystis aeruginosa (Kützing) Kützing, Microcystis pulverea (H.C.Wood) Forti. in the presence of basalt tuff. The possibility of using basalt tuff as an adsorbing material for regulating the number of cyanobacteria and preventing toxic “water bloom” in fluid circuits. The study analyzes the change in the biomass amount and the dynamics of the proportion of dead cyanobacteria in response to the presence of basalt tuff in the culture medium. It is noted that the use of basalt tuff leads to a decrease in the growth activity of cultures of Microcystis aeruginosa, Microcystis pulverea, manifested in an increase in the number of dead cells and slowdown the accumulation of cyanobacterial biomass. These effects are the result of a decrease in the amount of available nitrogen in the nutrient medium.
Various studies of the components of the antioxidant protection system of microalgae D. armatus under the influence of osmotic stress and active forms of oxygen will allow to develop methods for controlling carotenogenesis in a given culture and to obtain carotenoid enriched feed for zooplankton. These studies made it possible to evaluate the activity of catalase, peroxidase enzymes in cells that are cultured under the induction of carotenogenesis by free radical oxidation promoters and osmotic stress on the background of physiological changes. It is established that under these conditions, there is an increase in volumes and aggregation of vegetative cells. At the same time, the amount of biomass remains at the level of the first day of inductors application. Against the background of a decrease in growth activity, a decrease in the number of metabolically active cells in cytochrome oxidase was observed. It is also shown that, when iron sulfate is introduced with hydrogen peroxide and sodium chloride against the background of enhanced carotenogenesis, antioxidant systems are activated by increasing the activity of catalase and peroxidase. Under such conditions, it is possible to achieve increased production of carotenoids in Desmodesmus armatus culture.
The nutritional composition and amino acid profile of Moina macrocopa were studied using different types of yeast (Saccharomyces cerevisiae and Rhodotorula glutinis) as fodder substrates. The effective accumulation of carotenoids in Moina macrocopa during R. glutinis yeast application was not accompanied by deterioration in the nutritional value of zooplankton. The content of total proteins and total lipids in Moina grown on S. cerevisiae and R. glutinis was not significantly different. However, the use of R. glutinis in the cultivation of M. macrocopa led to the change in the ratio of proteinogenic amino acids in the studied cladocerans. In particular, the share of methionine, leucine and isoleucine significantly increased. It allowed to enhance the quality of protein in the fodder zooplankton, that is especially important in the feeding of fish fry.
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