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1

Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and their intergeneric hybrids, revealed by FISH and GISH

100%
Ksiazczyk T., Taciak M., Zwierzykowski Z.
Journal of Applied Genetics
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2010
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tom 51
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nr 4
This study focuses on the variability of chromosomal location and number of ribosomal DNA (rDNA) sites in some diploid and autotetraploid Festuca pratensis and Lolium perenne cultivars, as well as on identification of rDNA-bearing chromosomes in their triploid and tetraploid F. pratensis × L. perenne hybrids. The rDNA loci were mapped using fluorescence in situ hybridization (FISH) with 5S and 25S rDNA probes, and the origin of parental genomes was verified by genomic in situ hybridization (GISH) with L. perenne genomic DNA as a probe, and F. pratensis genomic DNA as a block. FISH detected variation in the number and chromosomal location of both 5S and 45S rDNA sites. In F. pratensis mostly additional signals of 5S rDNA loci occurred, as compared with standard F. pratensis karyotypes. Losses of 45S rDNA loci were more frequent in L. perenne cultivars and intergeneric hybrids. Comparison of the F. pratensis and L. perenne genomes approved a higher number of rDNA sites as well as variation in chromosomal rDNA location in L. perenne. A greater instability of F. pratensis-genome-like and L. perenne-genome-like chromosomes in tetraploid hybrids was revealed, indicating gains and losses of rDNA loci, respectively. Our data indicate that the rDNA loci physically mapped on chromosomes 2 and 3 in F. pratensis and on chromosome 3 in L. perenne are useful markers for these chromosomes in intergeneric Festuca × Lolium hybrids.
2

Seed coat patterns in rapid-cycling Brassica forms

100%
Karcz J., Ksiazczyk T., Maluszynska J.
Acta Biologica Cracoviensia. Series Botanica
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2005
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tom 47
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nr 1
The micromorphological typology of seed surfaces was investigated in rapid-cycling Brassica (RCBr) forms, using scanning electron microscopy. Four types of basic ornamentation pattern were recognized: reticulate (B. rapa, B. juncea), reticulate-foveolate (B. nigra), randomly reticulate (B. oleracea, B. napus) and reticulate-rugose (B. carinata). The seed coats showed variation in the shape and size of the testa epidermal cells and the structure of the outer periclinal and anticlinal cell walls. The surface patterns in RCBr seeds were less exposed and were moderately reticulated compared to cultivated Brassica species. The micromorphological characteristics of the seed coats may provide valuable additional diagnostic criteria for delimitation of RCBr forms, and can be used in identification of seeds.
3

FISH and GISH analysis of Brassica genomes

100%
Hasterok R., Ksiazczyk T., Wolny E., Maluszynska J.
Acta Biologica Cracoviensia. Series Botanica
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2005
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tom 47
|
nr 1
Fluorescence and genomic in situ hybridization (FISH and GISH) methods were used for discrimination of Brassica genomes. The three diploid and three allotetraploid species of Brassica, known as the "U-triangle," represent an attractive model for molecular and cytologieal analysis of genome changes during phylogeny in the genus Brassica. The use of genomic DNA probes enabled unambiguous discrimination of the ancestral genomes in B. juncea and B. carinata, and was only partially successful in B. napus. GISH signals in all genomes were localized predominantly in pericentromeric regions of chromosomes. Simultaneous application of genomic and ribosomal DNA probes in multicolor GISH and FISH allowed identification of a significant number of chromosomes in the B. juncea complement. The study also revealed that species of Brassica possess Arabidopsis-type telomeric repeats which in all genomes occupied exclusively terminal, that is, telomeric, locations of chromosomes.
4

A large-scale survey of genetic variation and genome evolution within the invasive Reynoutria complex

88%
Bzdega K., Janiak A., Ksiazczyk T., Sliwinska E., Tokarska-Guzik B.
Biodiversity: Research and Conservation
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2014
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tom Suppl.1
5

Identification of the chromosome complement and the spontaneous 1R/1V translocations in allotetraploid Secale cereale × Dasypyrum villosum hybrids through cytogenetic approaches

76%
Ksiazczyk T., Apolinarska B., Kulak-Ksiazczyk S., Wisniewska H., Stojalowski S., Lapinski M.
Journal of Applied Genetics
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2011
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tom 52
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nr 3
Genome modifications that occur at the initial interspecific hybridization event are dynamic and can be consolidated during the process of stabilization in successive generations of allopolyploids. This study identifies the number and chromosomal location of ribosomal DNA (rDNA) sites between Secale cereale, Dasypyrum villosum, and their allotetraploid S. cereale × D. villosum hybrids. For the first time, we show the advantages of FISH to reveal chromosome rearrangements in the tetraploid Secale × Dasypyrum hybrids. Based on the specific hybridization patterns of ribosomal 5S, 35S DNA and rye species-specific pSc200 DNA probes, a set of genotypes with numerous Secale/Dasypyrum translocations of 1R/1V chromosomes were identified in successive generations of allotetraploid S. cereale × D. villosum hybrids. In addition we analyse rye chromosome pairs using FISH with chromosome-specific DNA sequences on S. cereale × D. villosum hybrids.
6
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Application of new biotechnologies in Brassica napus L. resynthesis

64%
Sosnowska K., Szala L., Liersch A., Mikolajczyk K., Ksiazczyk T., Bocianowski J., Wos H., Cegielska-Taras T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2015
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tom 96
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nr 1
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