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Cynopterus sphinx is a frugivorous bat also known to feed regularly on leaves. This raises the question whether microorganisms capable of digesting such a diet are present in the gut. In the present study cellulolytic and xylanolytic bacteria were isolated from the intestine of C. sphinx on Berg's agar medium containing carboxymethyl cellulose and xylan. The isolated cultivable cellulose and xylan degrading bacteria were characterized biochemically and identified to be Bacillus sp., Clostridium sp., Streptococcus sp., and Staphylococcus sp. Among the cultivable bacteria from the intestine of the insectivorous Hipposideros fulvus, which was used as a control, no cellulolytic and xylanolytic bacteria could be isolated. We hypothesize that leaves could be a carbohydrate source for bats. By isolating polysaccharide-degrading bacteria from the intestine of C. sphinx, we infer that they help in digestion of xylan and soluble cellulose in their diet.
Background. Metals discharged in wastes from different industries into wetland ecosystems pose a serious threat to the receiving environment. Biotic components grown in the area amass such metals within their body through nutritional uptake and accumulation. The present investigation was carried out to study the extent of accumulation of different waste metals in tissues of cichlids commonly cultured in composite industrial wastewater-fed fishponds (Bheris) in East Calcutta Wetlands (ECW), a Ramsar site in India. Materials and Methods. AAS studies were employed to estimate metal (Cr, Mn, Fe, Cu, Zn, and Pb) concentrations in water, and bottom sediment of fishponds and fish tissues (gill, liver, muscle, and bones) of cichlids: Nile tilapia, Oreochromis niloticus L. and Mozambique tilapia, O. mossambicus (Peters, 1852), collected from contaminated and control sites. Results. The concentrations of metals in bottom sediment were found higher than those of the overlying water. It was evident from the experiment that, either bone or liver was the site of maximum accumulation for the elements while muscles were the site of least metal accumulation in both fish species. Chromium, which was a highly publicised contaminant of tannery effluents, showed a similar distribution pattern (muscle < gill < liver < bone) in these fishes. Concentrations of non-essential metals like Pb were highest in Nile tilapia liver (17.9 mg • kg-1) followed by Mozambique tilapia liver (14.7 mg • kg-1). Principal component analysis with Varimax rotation revealed that Fe and Mn were in the first and second component, respectively, to influence metal uptake and bioaccumulation. Conclusion. Tissue-specific and species-specific patterns of metal concentration and partitioning were apparent from our experimentation. Dendrograms showed two definite clustering pattern, gill and muscle in one cluster with bone and liver in the other, two important organs so far as partitioning (in bone) and detoxification (in liver) were concerned.
Gene flow results from movement between populations, homogenising gene pools and impacting genetic variation and evolution. Growing evidence suggests that movement of individuals among populations may be more strongly determined by ecological traits. We compared genetic and morphometric differentiation in four species of bats — Eonycteris spelaea, Cynopterus sphinx, Rhinolophus affinis and Hipposideros pomona — which differ in their flight capability and roosting requirement to understand their effects in shaping genetic structure. Hypervariable region I of the mitochondrial control region was amplified from 40 E. spelaea, 28 C. sphinx, 44 R. affinis and 57 H. pomona sampled from locations spread across the Andaman archipelago. Populations of E. spelaea were nearly panmictic; R. affinis were differentiated into two clusters, and H. pomona were differentiated into three clusters. Consistent genetic and morphometric clusters were obtained for C. sphinx and the genetic break for C. sphinx occurs between Middle and South Andaman Islands, coincided with the Jarawa Tribal Reserve. In conclusion, poorly-dispersing, caveroosting species show high population structure, but when flight capability is very well-developed, the effect of disjunct roost availability is offset. The genetic structure of C. sphinx where we expected panmixia, is possibly confounded by the colonization history of its two genetic lineages and its habitat use which may prevent significant gene flow between the two lineages.
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