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This study was conducted to evaluate the influence of boron (B) application through seed coating on leaf elongation, tillering, water relations, panicle sterility, kernel yield, and grain biofortification of fine grain aromatic rice. Boron was applied as seed coating at 1.0, 1.5, 2.0, 2.5, and 3.0 g B kg-1 seed in two rice cultivars Super Basmati and Shaheen Basmati. Boron seed coating significantly affected the leaf elongation, water relations, panicle fertility, kernel yield and grain biofortification in both rice cultivars. However, seed coating with 1.0–2.0 g B kg-1 seed was effective in improving the leaf emergence and elongation, and tillering. Whereas water relations (water and osmotic potential) were improved by all B seed coating treatments, but pressure potential was only improved from seed coating with 2.0–3.0 g B kg-1 seed. Kernel yield was improved by all B seed coating treatments; however seed coating with 2 g B kg-1 seed was the most effective treatment in this regard. Increase in kernel yield, by B seed coating, was attributed to decrease in panicle sterility. Leaf and kernel B contents were increased with increase in B concentration in seed coating.
The influence of pre-sowing seed treatments on germination, seedling growth, ion accumulation and plant antioxidant system in spring wheat (Triticum aestivum L.) cultivar MH-97 was examined under control and saline conditions. Albeit, all pre-sowing seed treatments were effective in improving seedling fresh and dry weight under saline conditions, the effect of hormonal priming with kinetin was very pronounced particularly in improving seedling vigor. The plants raised from seeds primed with kinetin have maximum root and shoot lengths under saline conditions while pre-sowing chilling treatment increased shoot length maximally whereas halopriming increased root length maximally under non-saline conditions. The Na⁺ uptake was decreased while K⁺ uptake was increased in plants due to hormonal priming with kinetin and hydropriming under stress conditions. Except halopriming, all seed treatments significantly increased leaf protein contents under non-saline conditions, however decreased under saline conditions. Halopriming resulted in a significant increase in protein concentration. Catalase (CAT) activity was significantly increased under salinity stress, however, hormonal priming and halopriming treatments maximally increased CAT activity. All the seed treatments were effective in increasing superoxide dismutase (SOD) level but hormonal priming with kinetin maximally increased its activity under stress conditions. Although all pre-sowing seed treatments were effective in alleviating the adverse effect of the salt stress on wheat plants, their effects on altering the concentration of different ions, seedling growth and plant antioxidant system were different in the wheat plants.
Drought stress hampers rice performance principally by disrupting the plant–water relations and structure of biological membranes. This study appraised the role of polyamines (PAs) in improving drought tolerance in fine grain aromatic rice (Oryza sativa L.). Three PAs [putrescine (Put), spermidine (Spd) and spermine (Spm)] were used each at 10 µM as seed priming (by soaking seeds in solution) and foliar spray. Primed and non-primed seeds were sown in plastic pots with normal irrigation in a phytotron. At four-leaf stage, plants were subjected to drought stress by bringing the soil moisture down to 50% of field capacity by halting water supply. For foliar application, 10 µM solutions each of Put, Spd and Spm were sprayed at five-leaf stage. Results revealed that drought stress severely reduced the rice fresh and dry weights, while PAs application improved net photosynthesis, water use efficiency, leaf water status, production of free proline, anthocyanins and soluble phenolics and improved membrane properties. PAs improved drought tolerance in terms of dry matter yield and net photosynthesis was associated with the maintenance of leaf water status and improved water use efficiency. Among the antioxidants, catalase activity was negatively related to H₂O₂ and membrane permeability, which indicated alleviation of oxidative damage on cellular membranes by PAs application. Foliar application was more effective than the seed priming, and among the PAs, Spm was the most effective in improving drought tolerance.
Environmental contamination, herbicide resistance development among weeds and health concerns due to over and misuse of synthetic herbicides has led the researchers to focus on alternative weed management strategies. Allelochemicals extracted from various plant species can act as natural weed inhibitors. In this study, allelopathic extracts from four plant species sorghum [Sorghum bicolor (L.) Moench], mulberry (Morus alba L.), barnyard grass [Echinochloa crusgalli (L.) Beauv.], winter cherry [Withania somnifera (L.)] were tested for their potential to inhibit the most problematic wheat (Triticum aestivum L.) weeds wild oat (Avena fatua L.) and canary grass (Phalaris minor Ritz.). Data regarding time to start germination, time to 50% germination, mean germination time, final germination percentage, germination energy, root and shoot length, number of roots, number of leaves, and seedling fresh and dry weight was recorded for both the weeds, which showed that mulberry was the most inhibitory plant species while sorghum showed least allelopathic suppression against wild oat. Mulberry extracts resulted in a complete inhibition of the wild oat germination. The allelopathic potential for different plants against wild oat was in the order: mulberry > winter cherry > barnyard grass > sorghum. Mulberry, barnyard grass and winter cherry extracts resulted in a complete inhibition of canary grass. Sorghum however exhibited least suppressive or in some cases stimulatory effects on canary grass. Plants revealing strong allelopathic potential can be utilized to derive natural herbicides for weed control.
This study was conducted to investigate the benefits associated with re-drying after seed priming with polyamines. Wheat (cv. AS-2002) seeds were soaked in 10 and 20 mg L⁻¹ aerated solutions of spermidine (Spd), putrescine (Put) and spermine (Spm), and distilled water (CK2) for 12 h at 28 ± 2°C. Untreated seeds (CK1) and priming in distilled water (CK2) were taken as control treatments. Seeds were primed in two sets: In one set, after each treatment, seeds were given three surface washings with distilled water and dried closer to original moisture; in the other, seeds were only surface dried and used immediately. Use of surface-dried seeds after priming was more effective since it reduced emergence time and synchronized the emergence. Moreover, final emergence, shoot and root length, seedling fresh and dry weight were also improved. Improved starch metabolism was considered possible reason of seed invigoration. All the seed treatments resulted in a lower electrical conductivity of seed leachates compared with control; however, there was more decrease in seeds re-dried after priming than the seeds surface dried after priming. Although the effect of all the polyamines was stimulatory, Spd was the more effective for most of the attributes studied. Nonetheless, Put was more effective for seedling fresh and dry weights. All the polyamines were more effective at lower concentrations except Spm, which improved the coefficient of uniformity of emergence at high concentration. To conclude, if immediate sowing is possible, use of surface-dried seeds after priming may be more effective; seed priming with 10 mg L⁻¹ Spd was the most effective technique when surface dried.
Suspended sediments load estimation in the Indus River and its important tributaries in Khyber Pakhtunkhwa, Pakistan is presented to determine siltation in major reservoirs in the area and to create a strategy to minimize and/or control this risk for the enhancement of hydel energy production, irrigation, and other domestic purposes. The study area is part of an orogenically active region along the northwestern collision zone of Indian-Eurasian plates. During spring and summer suspended sediment load is highest, but least in winter. Sediment load of all rivers studied is directly proportional to the melting of glaciers and/or high rainfall during spring and summer. Glacier melt, rainfall, lithology of rocks (shale, slate, phyllite, mudstone, and siltstone), discharge, and other anthropogenic factors caused the high suspended load in rivers. Mostly the fine sediments settle during winter due to flow velocity of rivers reducing appreciably and residence time of water increasing in reservoirs. The high sediment influx deposition in major reservoirs like the Terbella and Warsak, etc. reduces their storage capacity as well as power generation capacity. So it is proposed that life span of these reservoirs can be lengthened by the construction of small check dams (silt control dams) on upstream, dam cleaning tactics, effective watershed management, extensive afforestation on the steep mountain slopes upstream of the lake, and the use of runoff rivers for electricity generation using steep gradient and fast flow velocity.
Sulphur (S) nutrition is very important for harvesting potential seed and oil yield of rapeseed. This study evaluated response of foliage applied thiourea on the performance of two canola cultivars Shiralee and Dunkeld. Sulphur was applied to soil (40 kg ha-1) or foliage (500 and 1,000 mg L-1) at rosette, bud initiation and flowering stages using elemental S or thiourea as source, respectively; no S application was taken as control. Among all the treatments, soil application of S improved the crop growth, yield and oil quality in both cultivars and was followed by foliar application of thiourea at 1,000 mg L-1 compared with no application. Soil applied S and foliar thiourea (1,000 mg L-1) delayed the flowering and maturity. Soil and foliar applied S significantly improved leaf area index, crop growth rate, net assimilation rate and chlorophyll contents. Plant height, number of branches, siliqua per plant, seed number per siliqua, 1,000-seed weight, biological and seed yield were also increased by soil applied S and foliage applied thiourea (1,000 mg L-1). Nonetheless, improvement in harvest index, seed oil, protein and glucosinolate contents was only observed from foliage applied thiourea (1,000 mg L-1). Response of cv. Shiralee to sulphur application was better than cv. Dunkeld. In conclusion, foliar applied thiourea (1,000 mg L-1) can have potential to improve growth, yield and oil quality in canola and can be economically viable and attractive alternative source.
Spray droplet size has long been recognized as an important variable that applicators of vector control sprays must be aware of to make the most effective spray applications. Researchers and applicators have several different techniques available to assess spray droplet size from spray nozzles. The objective of this study was to compare the droplet size spectrum produced by three nozzles commonly used in vector control in a high-speed wind tunnel, when characterized using three different laser-based droplet size measurement systems. Three droplet sizing systems: Malvern Spraytec laser diffraction, Sympatec HELOS laser diffraction, and TSI Phase Doppler Particle Analyzer (PDPA), were simultaneously operated, but under different operating conditions, to measure the spray droplet size-spectra for three spray nozzles. The three atomizers: a TeeJet® 8001E even flat fan nozzle, a BETE® PJ high pressure fog nozzles, and a Micronair ® AU5000 rotary atomizer were evaluated in a high speed wind tunnel at airspeeds of 53 and 62 m/s (120 and 140 mph). Based on the results of this work, only the BETE® PJ high pressure fog nozzles met the label requirements for both Fyfanon® and Anvil®. While the other nozzle might met the Dv0.5 (VMD - volume median diameter) requirement for Fyfanon®, the resulting Dv0.9 values exceeded labeled size restrictions. When applying Anvil with the BETE PJ high pressure fog nozzles, it is important to use the smaller two orifice sizes. The larger sizes tended to result in Dv0.9 values that exceeded label recommendations.
In the current study, potential involvement of anthocyanins in the defence of the Zn hyperaccumulator plant, Noccaea caerulescens, was investigated in two independent glasshouse experiments. Plants were grown in compost amended with a range of Zn concentrations. In the first experiment, foliar herbivory was simulated by clipping the shoots with scissors, whilst in second experiment, plants were subjected to natural herbivory by thrips (Frankliniella occidentalis). In both experiments, foliar Zn concentrations were approximately twice as high as that applied to the soil, indicating accumulation occurred. In the clipping experiment, foliar anthocyanin concentrations decreased by 22–45 % with increasing soil Zn amendments and clipping increased anthocyanin concentrations by 17–24 % relative to unclipped plants. In a thrips feeding experiment, Zn treatment had little effect on anthocyanin content although there was a trend towards increased anthocyanin production in plants grown at the lower concentrations of Zn. Anthocyanin concentration increased by 49–52 % in N. caerulescens leaves subjected to thrip attack compared to that of thrip-free shoots. Thus, anthocyanins increased as a result of foliar damage (manual clipping and natural herbivory) and generally decreased with enhanced Zn concentrations. Foliar glucosinolate concentrations were higher in ungrazed leaves than in grazed leaves of the same thrip-infested plants, whilst anthocyanin production was higher in the grazed leaves. The possibility of an interaction between glucosinolate and anthocyanin production resulting from herbivory is discussed.
Water extracts from allelopathic crops possess the potential to control weeds effectively, especially when used in combination with reduced rates of herbicides. Label doses of different herbicides and their seventy percent reduced doses, were combined with 18 l/ha each of allelopathic sorghum and sunflower water extracts (WE). This combination was sprayed 30 days after sowing (DAS) for weed control in wheat (Triticum aestivum). Maximum reduction in total weed density and dry weight over the control, was recorded in a field sprayed with mesosulfuron + idosulfuron (Atlantis 3.6 WG) at 14.4 g active substance (a.s.)/ha. However, sorghum + sunflower WE each at 18 l/ha combined with doses which had been reduced by 70% of mesosulfuron + idosulfuron (Atlantis 12 EC at 36 g a.s./ha), or metribuzin + phenoxaprop (Bullet 38 SC at 57 g a.s./ha) or mesosulfuron + idosulfuron (Atlantis 3.6 WG at 4.32 g a.s./ha), reduced total weed dry weight by more than 90%, over the control. Sorghum and sunflower water extracts each at 18 l/ha combined with metribuzin + phenoxaprop (Bullet 38 SC at 57 g a.s./ha) produced a maximum number of productive tillers, spikelets per spike, number of grains per spike, biological yield and grain yield. Moreover, this treatment was the most economical along with having the maximum net benefits. The results suggested that weeds can be controlled in wheat, for a higher yield, when a 70% reduced herbicide dose is used in combination with allelopathic sorghum and sunflower water extracts.
Soil samples from petroleum-contaminated soil were collected from 25 different petroleum filling stations and automobile workshops in the district of Swat, Khyber Pakhtunkhawa, Pakistan. A total of seven bacterial genera were isolated. All of the isolates were Gram-positive bacteria. The genera identified by the culture and cell morphological characteristics were: Bacillus, Streptococcus, Staphylococcus, Micrococcus, Corynebacterium Arthrobacter, and Streptomyces. Lipolytic and saline activities of the selected isolates were studied. Among the isolates, Arthrobacter, Staphylococcus, Bacillus, Micrococcus, Corynebacterium, and Streptomyces produced lipase enzymes, while no lipase was produced by Streptococcus. Dense growth of Bacillus and Streptococcus was observed at 1% NaCl. Dense growth of Streptomyces was observed at strength of 2% NaCl. At 3% NaCl concentration, dense growth of Staphylococcus, Micrococcus, Corynebacterium, and Arthrobacter was observed, indicating that they were moderately halotolerent. In our study, Bacillus, Arthrobacter, and Streptomyces showed optimum growth at pH 8.0, and Streptococcus, Staphylococcus, and Micrococcus showed optimum growth at pH 7.0. Only Corynebacterium showed optimum growth at pH 9.0, indicating that it is tolerant of higher pH conditions.
The indiscriminate use of herbicides has led to the contamination of water bodies, possibly affecting the health of aquatic biota, especially fish. Atrazine is considered as toxicants for aquatic fauna, due to its high persistence in soil, high half-life and high mobility toward aquatic bodies as well as high solubility in water. The objective of the present study was to determine (LC50) and to evaluate the acute and chronic toxicity of atrazine on the biochemical parameters; total protein and serum albumin of freshwater grass carp (Ctenopharyngodon idella). Above 15 μlL⁻¹, the LC50 was recorded revealing sensitivity of grass carp to atrazine. Grass carp was exposed to atrazine for 01 (15 μlL⁻¹), 02 (13 μlL⁻¹), 03 (10 μlL⁻¹), and 04 (08 μlL⁻¹) days/concentration for scrutinizing acute toxicity. Likewise, fish were exposed to atrazine for 10 (06 μlL⁻¹), 15 (04 μlL⁻¹), and 25 (02 μlL⁻¹) days/concentration for scrutinizing chronic toxicity. Control group concentration was 8.3 gL⁻¹ and 3.5 gL⁻¹. Total protein concentration observed for acute toxicity was 7.5 gL⁻¹, 6.5 gL⁻¹, 4.6 gL⁻¹, and 3.2 g/L and serum albumin concentration was 2.7 gL⁻¹, 1.6 gL⁻¹, 1.4 gL⁻¹, and 1.1 gL⁻¹ respectively. Similarly total protein concentration observed for chronic toxicity was 8.2 gL⁻¹, 6.8 gL⁻¹, and 4.3 gL⁻¹ and serum albumin concentration was 2.1 gL⁻¹, 1.7 gL⁻¹, and 1.4 gL⁻¹ respectively. Markedly decline (denoted by P<0.05, P≤0.01 and P≤0.001) was noticed in both the parameters concentration during acute as well as chronic toxicity, when compared with control group concentration, indicating negatively impinge of atrazine on grass carp as well as atrazine present in aquatic bodies must jeopardize the health of other aquatic fauna.
Upper Tanawallies is in the middle of the Western part of Hazara division, and includes the remote areas of four districts i.e. Mansehra, Abbottabad, Haripur and Tor-Ghar, having latitude 34°.34.40´ N to 34°.48.88´ N and 72°.84.27´ E to 73°.10.50´ E longitude. This area is loaded with plant diversity, and tree species in this area vary in their life form, leaf spectra and phenological behavior. There is no prior record of plants diversity in the area, hence, this study was conducted to explore the tree diversity, life form, leaf spectrum and phenology of the tree flora. A total 127 sampling stands (10 x 10 m2 ) were put into place in different locations in Upper Tanawallies to collect field data using the quadrat method. As an outcome, we learned that the region hosts 53 different tree species of 39 genera belonging to 25 tree families. The biological spectra of the trees were constructed according to Raunkiaer (1934). Results showed that Mesophanerophytes was the dominant life form class, contributing 34 (64%) of all tree species encountered, while Mesophyll and Microphyll was the dominant leaf size spectrum classes, each contributing to a count of 18 (34%) of all tree species. Frequent field visits were also carried out during the flowering and fruiting seasons in 2016/17. The result indicates that most tree species of the area show flowering during April-May (32%) while, maximum fruiting were recorded in June-July (36%). Our study concludes that anthropogenic activities on these forests should be reduced to overcome deforestation. This work will be the baseline for new research in the study area.
The purpose of the present study was to scrutinize the effect of glyphosate and atrazine (herbicide) on blood biochemical and hematological parameters of common carp, (Cyprinus carpio), including plasma glucose (RBS), cholesterol (CH), serum protein (SP), creatinine phosphates (CPK), lactate dehydrogenase (LDH), WBC, hemoglobin (Hb), platelets (PT), lymphocytes (LP), monocytes (MT), esinophils (EN), and neutrophils (NT), and on behavioral aspects for (24, 48, 72, 96) hours under doses of (0.1, 0.07, 0.05, 0.02) and (0.2, 0.15, 0.1, 0.05)mlL-1 respectively of glyphosate and atrazine. For analysis of biochemical and hematological parameters, the protocol of biochemical analyzer set (Merck Micro Lab 300 biochemistry analyzer) and hematological analyzer (Mindray BC-2300 Hematology Analyzer) was followed in the laboratory. An upturn in RBS, CH, and WBC concentration was observed while SP, LDH, LT, MT, and EP concentrations were decreased against both herbicides. CPK and Hb concentrations were increased against atrazine, while against glyphosate the concentrations were decreased. PT and NT showed momentous upturn in concentrations against glyphosate, while showing a decline against atrazine. Both herbicides affected the blood biochemical and hematological parameters of the selected fish. Behaviorally, changes were observed against both herbicides, including loss of equilibrium, increase in the frequency of opercular movements, fast swimming and jumping, losing balance, becoming exhausted and lethargic, vertical swimming, and bleeding at the base of the eyeballs.
The present study contemplates the enzymatic profile of grass carp, including lactate dehydrogenase (LDH), creatinine phosphokinases (CPK), serum glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase (Alk Phosp) under atrazine’s acute toxicity effects (LC50) for 01 (15 μl/L), 02 (13 μl/L), 03 (10 μl/L), and 04 (08 μl/L) days/concentration, respectively. For analyzing the enzymatic profile we followed the biochemical analyzer set protocol (Merck micro lab 300 biochemistry analyzer) in the laboratory. Control group concentrations for LDH, CPK, SGPT, and Alk Phosp were 342 IU/ml, 7513.3 IU/ml, 46 mmol/l, and 126.6 IU/ml, respectively. After treatment LDH concentrations were 906, 851, 765, and 545 IU/ml, respectively. CPK concentrations were 1,737, 2,445, 3,718, and 5,767 IU/ml, respectively. SGPT concentrations were 27, 24.3, 13.67, and 8.67, respectively, and Alk Phosp concentrations were 50.3, 30, 22.3, and 17.6 IU/ml, respectively. Maximum inclined (P≤0.001) in concentration of LDH was observed after 24 hrs exposure because of hepatic tissue damage, resulting in increased membrane permeability causing enhanced leaching out of LDH and as LDH participates in an anaerobic pathway, so increase LDH mean increases of anaerobic metabolism resulting from depletion of energy under environmental stress conditions by atrazine, while other enzymatic components like CPK, SGPT, and Alk Phosp showed kindred attributes in their result, like all parameter concentrations showed perpetual decline (P≤0.001) in their concentrations indicating reduced enzymatic activity due to a reduction in permeability for these enzymes, forcing the enzymes to accumulate in the cells as well as decrease in enzyme synthesis due to intoxication of atrazine.
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