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Professor Maria Rudawska

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The effects of tree genotype on below−ground ectomycorrhizal fungal communities of 40 years old European larch from Kowary, Bliżyn, Świętokrzyski National Park and Szczytna Śląska origins was investigated. The research was carried out in the provenance trial in the Experimental Forestry Unit in Krynica Zdrój (Beskid Sądecki Mountains, S Poland). Ectomycorrhizal fungal communities were assessed by a combination of morphological and molecular techniques. In total 22 fungal taxa has been distinguished (from 17 to 20, depending on larch provenance). Thirteen ectomycorrhizal fungal taxa were common to all analysed origins. The mean taxa richness, Shannon diversity and Simpson dominance coefficient did not differ significantly between provenances. Based on fungal taxa composition (Jaccard coefficient) tested larch origins were characterized by a high level of similarity of ectomycorrhizal fungal communities. Six detected fungal taxa have not been previously reported as symbiotic partners of European larch.
Hymenochaete cruenta is a saprotrophic fungus, grown mainly on the bark of dead branches and trunks of Abies trees and thus the distribution of this fungus in Poland and other European countries is closely connected with natural range of silver fir. Despite its wide range, H. cruenta is considered as rare in the whole area of its occurrence. In this paper, we present a new locality of H. cruenta, discovered in May and September 2016 in the Olbina nature reserve (southern Wielkopolska Lowland, Kalisz Forest District). Ecological notes and macro- and microscopic feature of basidiocarps of this fungus are presented. Additionally, the current distribution of H. cruenta in Poland comprising 78 localities is provided. The rank of H. cruenta among threatened species categories in different European countries is discussed. It seems that the species is rare and threatened in Poland, and it should be still classified as “vulnerable” on the red list of macrofungi. The significance of H. cruenta for diversity of mycobiota and the necessity of further research on the dynamic of occurrence of H. cruenta inside and outside of the natural range of A. alba is underlined.
Biomass of extramatrical mycorrhizal mycelium (EMM) was examined under canopies of mature Norway spruce trees grown in different forest stands in Poland. Two mountain forest sites (Brenna and Salmopol), one upland site (Zwierzyniec) and one lowland site (Mirachowo) have been investigated, using sand-filled mesh-bags method. The in-grow mesh-bags were buried in the soil for 12 months (since October up to the next October) or for 4 months (since June up to October) at four depths at each site: 5, 15, 30 and 45 cm (Brenna and Salmopol) or 60 cm (Zwierzyniec and Mirachowo). The mycelium biomass was estimated from the ergosterol content determined in the mesh-bags. The results indicated significant differences in EMM production and their vertical distribution between the mountain and the upland and lowland forest sites. The lowest EMM biomass was found at the experimental plot in the mountainious site Brenna. Considerable decrease of EMM biomass with the soil depth was recorded after 12 months of the mesh-bags incubation in soil in the upland and lowland sites, while in the mountain forests decrease of the EMM biomass in the lower soil depths diminished more gradually EMM biomass determined in the mesh-bags placed in soil at the upper 5 and 15 cm tended to be higher after 4 months than after 12 months of incubation period. Such results suggest that the time necessary for evaluation of EMM biomass in soil may be limited to the summer–autumn months, when the production of EMM is the highest. Variable stress factors can influence decreased ectomycorrhizal mycelium production and/or their destruction. Further research in different forest types and regions are needed for better understanding factors determining EMM biomass production and surviving in soil.
Pedunculate oak (Quercus robur L.) is among the major managed tree species in Europe. In Poland, oak woodlands cover 6.9% of forest area. Furthermore, the significance of native oaks for Polish forestry is rising because the Polish National Forest Strategy predicts a growth of forest cover from 28.5% to 33% by year 2050 with the primary focus on the increase in contribution of deciduous tree species (from 22% to 33%). Therefore, for establishment of new stands, oak seedlings are grown for 2−3 years in forest nurseries. For optimal growth and development all European oak species are critically dependent on ectomycorrhizal fungi. The objective of this study was to describe the temporal dynamics of ectomycorrhizal community of pedunculate oak seedlings during the first year of growth in bare−root forest nursery. Experiment was conducted in forest nursery Miranowo (western Poland). The species structure of an ectomycorrhizal community was assessed every 10 or 15 days from May till October. Mycorrhizal colonization of tested seedlings changed from 0 to 85% depending of the time of sampling. Based on combination of morphological and molecular techniques, nine ectomycorrhizal fungal taxa were distinguished. The first ectomycorrhizas formed by Scleroderma verrucosum were detected 50 days after acorns sowing. In the next sampling days ectomycorrhizal community was dominated consecutively by Inocybe curvipes and Hebeloma sacchariolens (with maximum of abundance 60.98 and 75.55%, respectively). The obtained results were strongly influenced by the infection with the fungal biotroph Erysiphe alphitoides and resulted in a dramatic reduction in the abundance of living ectomycorrhizas down to 7.5%. Our investigation revealed that ectomycorrhizal fungal community of pedunculate oak seedlings during the first year of growth in bare−root forest nursery is rich in terms of number of species and a temporal changes are very dynamic. Thus we suggest, that in case of pedunculate oak artificial inoculation in general is not necessary for oaks seedlings in nursery practice.
Indigenous mycorrhizal colonisation of one (1/0) and two (2/0) year old Scots pine (Pinus sylvestris L.) seedlings from forest nurseries situated in the north-west part of Poland was investigated. Seedlings were fertilized after a schedule designed to satisfy their requirements which resulted from soil analysis of each nursery. In autumn needle nitrogen concentration and mycorrhizal status were estimated. As a rule seedlings 1/0 obtained high doses of fertilizers, and their needle nitrogen concentration highly exceeded the recommended optimal level above which mycorrhiza formation is greatly restricted. Highly negative correlation was found between the nitrogen status of foliage and mycorrhizal quantitative and qualitative colonisation estimated in the abundance class of 0-5. When internal nitrogen level was low, several ectomycorrhizal morphotypes were found on roots of tested plants. Excessive nitrogen fertilization decreased root ramification pattern and along with increased pH promoted ectendomycorrhizal symbiosis. Two-year-old seedlings (2/0) were characterised by significantly lower nitrogen concentration in the needles which resulted in much higher ectomycorrhizal colonisation. Ectendomycorrhizal symbiosis in the case of 2/0 seedlings was scarce. The results indicate that natural colonisation in nurseries is dependent on the soil management practices, especially nitrogen fertilization. Optimalization of nitrogen fertilization may intensify ectomycorrhizal colonisation of the nursery stock from indigenous fungal species and improve the growth of Scots pine seedlings in bare-root nurseries and plantations.
Ectomycorrhizas of Scots pine (Pinus sylvestris L.) and beech (Fagus sylvatica L.) were sampled in a mature forest ecosystem exposed for more than 40 years to moderate levels of gaseous and dust pollutants. Soil of the forest site was characterised by low pH and accumulation of heavy metals (Pb, Mn, Zn, Cu, Cd, Fe). Mycorrhizal vitality and enzyme activity of the root-surface and soil acid phosphatase (AcPase) were studied at 17 measurement points (0–5 cm soil depth) in relation to the content of inorganic phosphate (Pi) and aluminium ions (Al3+) in the soil. Anatomy of Scots pine and beech mycorrhizas taken from different measurement points was observed. The concentration of essential nutrients (C, N, P, Ca, Mg) and the ratios Ca/Al, Mg/Al and N/P were analysed in fine roots. High concentrations of Al3+ in the soil (40–118 meq kg-1) and low levels of Pi (12–44 mg P2O5 kg-1) were accompanied by high activity of the root-surface AcPase of pine and beech mycorrhizas (25–67 and 33–86 μmol pNP g-1 fresh weight h-1, respectively) and soil AcPase (6.8–22.4 μmol pNP g-1 dry weight h-1). The results indicate that fine tree roots are undoubtedly under stress as evidenced by a disturbance in P uptake and accumulation. However, the high vitality of mycorrhizas and the high Ca/Al ratio in fine roots suggest that the defence mechanisms of mycorrhizas and the rhizosphere are still able to ameliorate the influence of anthropogenic pollution.
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