BACKGROUND AND AIMS: Hypothesis that multiple sclerosis (MS) may be caused by chronic cerebrospinal venous insufficiency (CCVI) has gained public interest from both patients and physicians. However, there’s still lack of evidence for it. We have investigated presence of neuronal demyelination and degeneration, similar to these found in MS, in rat model of CCVI created by occlusion of jugular veins (JVs). METHODS: Twenty-five young female Wistar C rats were used. Complete ligation of both JVs (BO group), left JV (UO), or partial ligation (stenosis resulting in ~70% reduction of blood flow) of both JVs were performed. Blood flow in JVs was measured with Laser Doppler Flow Assessment. Neurological assessment using Neurologic Deficit Scale (NDS), 5-point EAE staging protocol, and gait analysis with CatWalk was performed. After 12 weeks, MRI for detecting demyelinating plaques as well as signs of bloodbrain barrier (BBB) disruption was performed. Histologic analysis of brain specimens was focused on markers of inflammation and demyelination. RESULTS: No neurologic deficits were found in all experimental animals. Both NDS, EAE and gait analysis did not differ from normal. MRI T2- and T1- weighted imaging as well as FLAIR sequence did not reveal any abnormalities in the brains of experimental rats. Histological analysis did not show any signs of inflammation or demyelination. CONCLUSIONS: Twelve-week CCVI in rats, both complete and partial, did not induce any changes resembling pathologies observed in MS. Therefore, linking CCVI with origin of MS remains controversial.
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