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This study aimed to assess lipid peroxidation and antioxidant enzymes in goats naturally infected with Babesia ovis. Red blood cell count (RBC), hemoglobin (Hb) concentration, packed cell volume (PCV), malondialdehyde (MDA) concentration, erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and total antioxidant capacity (TAC) were determined in 15 goats naturally infected with B. ovis as well as same number of healthy goats. The parasitological diagnosis was confirmed using polymerase chain reaction (PCR) analysis by amplifying a partial 18S rRNA gene sequence of B. ovis. Percentage of parasitemia varied from 0.01 to 1%. The activities of erythrocyte GSH-Px, SOD, CAT and TAC were significantly lower (p<0.05) in the infected goats than in healthy ones. MDA concentration in erythrocytes of infected goats was significantly higher in infected goats than in healthy ones (pš0.05). Severity of parasitemia showed a positive correlation with the MDA and negative correlation with PCV, SOD, CAT, GSH-Px and TAC. Also, MDA was negatively correlated with PCV, SOD, CAT, GSH-Px and TAC. The results of this study suggested that oxidative damage to RBCs may contribute to the pathogenesis of anemia in caprine babesiosis.
Many species of animals play the role of intermediate host for Neospora caninum. Although the neosporosis has a global distribution, however there is no information on goats’ infection in Iran. The main goal of present study was to determine the seroprevalence of N. caninum in goats of western Iran. A total of 450 sera were taken and analyzed for antibodies to N. caninum using ELISA technique. Of those, 28 (6.2%) examined goats were found to be seropositive (95%, CI=6.2±2.2%). In which male goats (11.1%) had the highest IgG against N. caninum with more than 2 years-old (54.4%). There was significant difference between prevalence and age. Positive association were in animals with history of abortion (18/30, 60%) and IgG against N. caninum. No correlations were found between male and female. This was the first report on N. caninum infection in Iranian goats and may be responsible for abortion and economic losses in goat husbandry in the region.
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