Wyniki wyszukiwania

1

Capsaicin-sensitive nerves in the control of visceral circulation

100%

Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

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2001

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tom 52

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nr 2

2

Badania biomedyczne na zwierzetach.

88%

Pawlik W. W.

Nauka. Polska Akademia Nauk

|

1995

|

nr 1

145-149

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Adenosine mediation of mesenteric blood flow

63%

Jacobson E. D., Pawlik W. W.

Journal of Physiology and Pharmacology

|

1992

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tom 43

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nr 1

The mesenteric circulation is regulated by multiple mechanisms, there is sufficient reason to support the suspicion that local metabolic factors are especially important in the control of intestinal vasculature. Of these, adenosine, a purine nucleoside and mesenteric vasodilator, may be the messenger of the intenstinal tissue to signal appropriate responses of the intestinal vessels. The evidence supporting the candidacy of the nucleoside as a local regular of mesenteric circulation may be summarized, as follows: Adenoside is present in the tissue of the gut in measurable quantities. Exogenous adenosine is a powerful dilator of mesenteric resistance vessels. Blockade of adenosine receptors in the mesenteric circulation interferes significantly with three autoregulatory phenomena, i. e., postprandial hyperemia, pressure-flow autoregulation, and reactive hyperemia. The evidence which weakens the role of adenosine as mesenteric vasoregulator includes: Findings in several reports that adenosine depressed intenstinal oxygen consumption. The failure of adenosine receptors to inhibit some autoregulatory hyperemias of the gut and the rather limited amount of evidence regarding tissue adenosine release in autoregulatory responses of the gut’s vasculature.

4

Monocytes and vascular endothelial cells apoptosis. Role of p-HSP27

51%

Gawad A., Ptak-Belowska A., Brzozowski T., Pawlik W. W.

Journal of Physiology and Pharmacology

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2009

|

tom 60

|

nr 4

55-61

The aim of this study was to find out whether stimulated monocytes could trigger apoptosis of vascular endothelial cells. Human umbilical vein endothelial cells (HUVEC) (EC) were co-cultured for 24 h and 48 h with monocytes isolated from peripheral blood (peripheral blood monocytes) or MonoMac6 cell line activated previously with proinflammatory cytokines. Real-time PCR was conducted to investigate p53 up-regulated modulator of apoptosis (PUMA), heat shock protein HSP70 and HSP27 genes expression. Changes in the level of PUMA, HSP70, HSP27 and phospho-heat shock protein 27 (p-HSP27) proteins were analyzed by means of immunoprecipitation. Apoptosis was determined by TUNEL and poli-(ADP ribose) polymerase ( PARP ) cleavage assay. In HUVEC cells stimulated with monocytes hardly any increase of PUMA mRNA was observed, but the PUMA protein level was significantly up regulated especially after 24 h. Heat shock proteins (HSP70 and HSP27) mRNA expression was elevated after 24 h and 48h and confirmatory up regulation of these proteins was observed in HUVEC cells stimulated with peripheral blood monocytes but not with MonoMac6 cells. Interestingly, in nuclear compartment of HUVECs exposed to the monocytic line and native monocytes, a significant increase of p-HSP27 level has appeared. TUNEL and PARP cleavage assay did not show any apoptotic HUVEC cells after stimulation with monocytes. The main observations of this study indicate that monocytes do not trigger apoptosis of vascular endothelial cells. Proapoptotic activation mediated by PUMA that was observed seemed to be counterbalanced by significant increase of antiapoptotic HSP70, HSP27 and especially phospho-HSP27 proteins level.

5

The role of gastrointestinal hormones in the regulation of intestinal circulation

51%

Walus K. M., Pawlik W. W., Konturek S. J.

Acta Physiologica Polonica

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1979

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tom 30

|

nr 1

6

Brain-gut axis in gastrointestinal system: Introductory remarks

51%

Konturek S. J., Pawlik W. W., Dajani E. Z.

Journal of Physiology and Pharmacology. Supplement

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2003

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tom 54

|

nr 4

3-7

7

Napoleon Cybulski - Polish pioneer in developing of the device for measuring blood flow velocity

51%

Pawlik W. W., Konturek S. J., Bilski R.

Journal of Physiology and Pharmacology. Supplement

|

2006

|

tom 57

|

nr 1

107-118

Napoleon Cybulski (1854-1919) was the most prominent Polish physiologist who chaired the Department of Physiology at the Faculty of Medicine of Jagiellonian University between 1885 and 1919. One of his greatest achievements was the construction of a device for precise measurements of blood movement in the vessels - the photohemotachometer, which provided a better insight into the physiology and pathophysiology of the circulatory system. In the field of endocrinology Cybulski together with Szymonowicz, found out that adrenal extracts contain biologically active substances that elevate blood pressure. He was also a constructor of an extremely clever microcalorimeter to measure the quantity of heat produced during isolated muscle contraction. He applied, for the first time, condensator discharges to stimulate nerves and analyzed changes in the excitability of the muscles. Cybulski proved that the cause of the electrical excitability of tissue depends on the electrical energy and the time of its duration. Together with Beck, he performed the experiments on the sensory centers in the central nervous system by means of the observations of the electrical evoked potentials. They discovered the continuous electrical oscillations in the brain - the brain waves and recorded the negative electrical potentials in certain brain areas induced by sensory impulses coming from the periphery. Using this technique Beck and Cybulski localized various centers in the brain of dogs and Macaccus rhesus monkeys. The names of Beck and Cybulski were quoted in many publications on neurophysiology and electroencephalography. The present paper describes Napoleon Cybulski as a constructer of an original instrument for studying the movement of blood under various experimental conditions an emphasizes the contribution of this invention to the development of physiology.

8

Systemic circulatory effects of pentagastrin

51%

Pawlik W. W., Gustaw P., Czarnobilski K., Konturek S. J.

Acta Physiologica Polonica

|

1987

|

tom 38

|

nr 5

9

Leptin is the modulator of HSP60 gene expression in AR42J cells

51%

Bonior J., Jaworek J., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

|

2006

|

tom 57

|

nr 7

135-143

Leptin, circulating protein involved in the control of body weight and energy expenditure received attention as a modulator of immune response of the organism. Leptin receptors have been detected in the pancreas and experimental studies have shown that leptin protects the pancreas against the damage induced by caerulein overstimulation. Heat shock proteins (HSP) are endogenous proteins produced by various cells exposed to high temperature or to the noxious agents. HSP protect the cells against various environmental and endogenous stressors. The implication of HSP60 in the leptin-induced pancreatic protection has not been examined yet. The aim of this study was: to investigate the changes of HSP60 mRNA signal in the pancreatic AR42J cells subjected to caerulein and leptin. AR42J cells were incubated in standart medium at 37°C for: 0, 1, 3, 5, 12 or 24 h, under basal conditions. Incubation time of 3 h was selected for the next experiments. AR42J cells were incubated in presence of caerulein (10-11, 10-9 or 10-7M), leptin (10-8 or 10-6M), or combination of above. Gene expression for HSP60 was determined by RT-PCR. The mRNA signal for HSP60 has been observed in AR42J pancreatic cells under basal conditions. Incubation of AR42J cells in presence of leptin (10-8 or 10-6M) resulted in the significant increase of gene expression for HSP60 in both groups of AR42J cells. Caerulein stimulation reduced mRNA signal for HSP60. The strongest mRNA signal for HSP60 has been observed after the exposition of AR42J cells to combination of leptin and caerulein. We conclude that: 1. Gene expression for HSP60 has been detected in pancreatic AR42J cells under basal conditions. 2. HSP60 gene expression was significantly increased in pancreatic AR42J cells stimulated by leptin whereas caerulein reduced this signal. 3. The strongest gene expression for HSP60 has been detected in the cells incubated with combination of caerulein and leptin.

10

Effect of prostaglandin I2 on gastric acid secretion and mucosal blood flow

51%

Pawlik W. W., Walus K. M., Konturek S. J.

Acta Physiologica Polonica

|

1979

|

tom 30

|

nr 1

11

Increase of heat shock protein gene expression by melatonin in AR42J cells

51%

Bonior J., Jaworek J., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology

|

2005

|

tom 56

|

nr 3

Heat shock proteins (HSPs) have been reported to protect the pancreatic cells from the acute damage produced by caerulein overstimulation. However the effects of caerulein, melatonin or hyperthermia preconditioning on mRNA signal for HSP60 in the pancreatic acinar cells has not been examined yet. The aims of this study were: 1. To investigate the gene expression for HSP60 in the pancreatic AR42J cells stimulated by melatonin, caerulein or combination of both these substances. 2. To compare above changes with mRNA signal for HSP60 in pancreatic AR42J cells subjected to hyperthermia preconditioning. AR42J cells were incubated in standard medium at 37°C for: 0, 1, 3, 5, 12 or 24 h, under basal conditions. Above cells were then subjected to heat shock (42°C) for 0, 1 or 3 h. In the next part of the study AR42J cells were incubated in presence of caerulein (10-11, 10-9 or 10-7M), melatonin (10-8 or 10-6M), or combination of above under basal conditions or following heat shock pretreatment. Gene expression for HSP60 was determined by RT-PCR. The mRNA signal for HSP60 has been observed in AR42J cells under basal conditions, and this signal was markedly and time-dependently increased in these cells subjected to hyperthermia preconditioning. Incubation of AR42J cells in presence of melatonin (10-8 or 10-6M) resulted in the significant and dose-dependent increase of gene expression for HSP60 in both groups of AR42J cells: preconditioned and in those, which were not subjected to hyperthermia. Caerulein stimulation reduced mRNA signal for HSP60. The strongest signal has been observed after the exposition of AR42J cells to hyperthermia preconditioning, combined with melatonin and caerulein. We conclude that: 1. Gene expression for HSP60 has been detected in pancreatic AR42J cells under basal conditions. 2. Hyperthermia preconditioning resulted in a significant and time-dependent increase of HSP60 signal in pancreatic AR42J cells. 3. HSP60 gene expression was significantly increased in pancreatic AR42J cells stimulated by melatonin whereas caerulein reduced this signal. 4. The strongest gene expression for HSP60 has been found in the cells subjected to the combination of hyperthermia preconditioning, caerulein and melatonin.

12

Nitric oxide as mediator of bradykinin-induced pancreatic circulatory and metabolic responses

51%

Pawlik W. W., Sendur R., Biernat J., Koziol R.

Journal of Physiology and Pharmacology

|

1997

|

tom 48

|

nr 4

13

Role of nitric oxide in the pancreatic vascular and metabolic responses associated with activation of capsaicin-sensitive neurons

51%

Pawlik W. W., Czarnobilski K., Sendur R., Konturek S. J.

Journal of Physiology and Pharmacology

|

1996

|

tom 47

|

nr 4

14

Badania medyczne na zwierzetach

51%

Pawlik W. W.

Wszechświat

|

1995

|

tom 96

|

nr 05

136-137

15

The effect of arachidonic acid on gastric secretion, mucosal blood flow and cyclic AMP concentration in gastric mucosa

45%

Mitis-Musiol M., Walus K. M., Pawlik W. W., Mikos E., Konturek S. J.

Acta Physiologica Polonica

|

1979

|

tom 30

|

nr 1

16

Endotoxemia in the infant rats modulates HSP60 protein level in the pancreatic acinar cells

45%

Bonior J., Jaworek J., Kot M., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

|

2007

|

tom 58

|

nr 3

189-198

17

Melatonin stimulates HSP27 phosphorylation in human pancreatic carcinoma cells [PANC-1]

45%

Leja-Szpak A., Jaworek J., Szklarczyk J., Konturek S. J., Pawlik W. W.

Journal of Physiology and Pharmacology. Supplement

|

2007

|

tom 58

|

nr 3

177-188

18

Effects of substance P on intestinal circulation and oxygen consumption

45%

Pawlik W. W., Gustaw P., Czarnobilski K., Sendur R., Konturek S. J.

Acta Physiologica Polonica

|

1987

|

tom 38

|

nr 5

19

Role of calcitonin gene related peptide in the modulation of intestinal circulatory, metabolic and myoelectric activity during ischemia-reperfusion

45%

Pawlik W. W., Obuchowicz R., Biernat J., Sendur R., Jaworek J.

Journal of Physiology and Pharmacology

|

2000

|

tom 51

|

nr 4,2

20

Adenosine modulates reactive hyperemia in rat gut

45%

Pawlik W. W., Hottenstein O. D., Palen T. E., Pawlik T., Jacobson E. D.

Journal of Physiology and Pharmacology

|

1993

|

tom 44

|

nr 2

Ograniczanie wyników

Capsaicin-sensitive nerves in the control of visceral circulation

Badania biomedyczne na zwierzetach.

Adenosine mediation of mesenteric blood flow

Monocytes and vascular endothelial cells apoptosis. Role of p-HSP27

The role of gastrointestinal hormones in the regulation of intestinal circulation

Brain-gut axis in gastrointestinal system: Introductory remarks

Napoleon Cybulski - Polish pioneer in developing of the device for measuring blood flow velocity

Systemic circulatory effects of pentagastrin

Leptin is the modulator of HSP60 gene expression in AR42J cells

Effect of prostaglandin I2 on gastric acid secretion and mucosal blood flow

Increase of heat shock protein gene expression by melatonin in AR42J cells

Nitric oxide as mediator of bradykinin-induced pancreatic circulatory and metabolic responses

Role of nitric oxide in the pancreatic vascular and metabolic responses associated with activation of capsaicin-sensitive neurons

Badania medyczne na zwierzetach

The effect of arachidonic acid on gastric secretion, mucosal blood flow and cyclic AMP concentration in gastric mucosa

Endotoxemia in the infant rats modulates HSP60 protein level in the pancreatic acinar cells

Melatonin stimulates HSP27 phosphorylation in human pancreatic carcinoma cells [PANC-1]

Effects of substance P on intestinal circulation and oxygen consumption

Role of calcitonin gene related peptide in the modulation of intestinal circulatory, metabolic and myoelectric activity during ischemia-reperfusion

Adenosine modulates reactive hyperemia in rat gut