Wyniki wyszukiwania

1

In vitro Study of biofilm growth on biologic prosthetics

100%

Bellows C., Smith A.

Polish Journal of Microbiology

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2014

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tom 63

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nr 4

Biologic prosthetics are increasingly used for the repair of abdominal wall hernia defects but can become infected as a result of peri- or early post-operative bacterial contamination. Data evaluating biofilm formation on biologic prosthetics is lacking. The aim of this study was to investigate the influence of different biologic prosthetics on the growth behavior of two different bacterial species and their ability to form biofilms. Methicillin resistant Staphylococcus aureus (MRSA) or Pseudomonas aeruginosa were incubated on disks of two biologic prosthetics-human acellular dermis (ADM), and porcine small intestinal submucosa (SIS). The bacteria were allowed to attach to the prosthetics and propagate into mature biofilms for 24 hours at 37°C. Images of biofilms were obtained using confocal microscopy and scanning electron microscopy (SEM). The number of viable cells and the biofilm biomass were quantified by colony forming units (CFUs) and crystal violet staining respectively. Analysis of variance was performed to compare the mean values for the different prosthetics. Each biologic matrix had a distinct surface characteristic. SEM visualized mature biofilms characterized by highly organized multi-cellular structures on surface of both biologic prosthetics. Quantification of bacterial growth over time showed that ADM had the lowest CFUs and biofilm biomass at 24 hours post-inoculation compared to SIS for both bacterial strains. MRSA and P. aeruginosa can form mature biofilms on biologic prosthetics but the relative abundance of the biofilm varies on different prosthetic constructs. Biologic material composition and manufacturing methods may influence bacterial adherence.

2

The neural correlates of cognitive time management: a review

100%

Rubia K., Smith A.

Acta Neurobiologiae Experimentalis

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2004

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tom 64

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nr 3

3

Presence of Acapoeta tanganicae (Actinopterygii: Cypriniformes: Cyprinidae ) within the Lake Rukwa catchment supports historic riverine connectivity with Lake Tanganyika

71%

Genner M. J., Turner G. F., Smith A., Mzighani S., Ngatunga B. P.

Acta Ichthyologica et Piscatoria

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2015

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tom 45

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nr 1

Freshwater fish biogeography can provide insight into past connectivity of river systems. Here we report the first discovery of the large-bodied cyprinid Acapoeta tanganicae (Boulenger, 1900) inside the Lake Rukwa catchment. Previously this species was thought to be endemic to Lake Tanganyika and immediately proximate sections of connected rivers. This shared distribution supports a scenario of direct connectivity of the two catchments during Holocene high stands of Lake Rukwa.

4

Caribanosis gen. nov. from Hispaniola (Pimeliinae: Stenosiini) with taxonomic notes on the tribes belopini and stenosini (Coleoptera: Tenebrionidae)

61%

Nabozhenko M., Kirejtshuk A., Merkl O., Varela C., Aalbu R., Smith A.

Annales Zoologici

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2016

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tom 66

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nr 4

Rhypasma Pascoe, 1862 is transferred from the tribe Stenosini (Pimeliinae) to Belopini (Lagriinae). Caribanosis gen. nov. is described and placed in Stenosini (Tenebrionidae: Pimeliinae) to hold Rhypasma quisqueyanus Garrido et Varela, 2011. The following new combination is established: Caribanosis quisqueyanus (Garrido et Varela, 2011), comb. nov. Caribanosis is similar to the South American genus Grammicus Waterhouse, 1845 but differs in having a single central pronotal keel, not two lateral keels as in Grammicus. Both are members of the subtribe Stenosina.

5

Effect of temperature stress on the endogenous cytokinin content in Arabidopsis thaliana [L.] Heynh plants

61%

Todorova D., Genkov T., Vaseva-Gemisheva I., Alexieva V., Karanov E., Smith A., Hall M.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 1

The levels of three endogenous cytokinin equivalents: zeatin (Z), iso-pentenyladenine (iP) and dihydrozeatin (dZ) in two Arabidopsis thaliana (L.) Heynh genotypes - wild type (wt) and ethylene-insensitive mutant (eti5), were compared using enzyme immunoassay (ELISA). Cytokinin content was measured after exposure to low (4 °C for 24 h in darkness) or high temperature (38 °C for 24 h in darkness). Measurements were performed immediately and 24, 48 and 120 h after treatments. It was found that at normal growth conditions eti5 plants contained more endogenous cytokinins compared to the wild type. At both temperature treatments mutant plants had decreased total cytokinin levels. Wild-type plants treated with high temperature (HT) exhibited reduced total cytokinins (with the exception of rates at 48 h), while low temperature (LT) treatment resulted in elevated total amount of the studied equivalents (except at 24 h). The obtained results suggested that HT had greater effect on cytokinin levels than LT since it caused more profound changes in the total content. We assume that this was due to the natural chilling tolerance of Arabidopsis plants.

6

Cytokinin oxidase-dehydrogenase [CKX] activity in wild and ethylene-insensitive mutant eti5 type of Arabidopsis thaliana [L.] Heynh plants and the effect of cytokinin N1-[2-chloro-4-pyridyl]-N3-phenylurea on enzymatic activity and leaf morphology

51%

Todorova D., Vaseva-Gemisheva I., Petrov P., Stoynova-Bakalova E., Alexieva V., Karanov E., Smith A., Hall M.

Acta Physiologiae Plantarum

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2006

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tom 28

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nr 6

The specific activity of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) (CKX) was determined in leaves of wild type (wt) and ethylene-insensitive mutant (eti5) of Arabidopsis thaliana (L.) Heynh plants. Comparative studies showed that this mutation has lower basal CKX activity than wt. Application of 4PU-30 (N1-(2-chloro-4-pyridyl)-N2-phenylurea) resulted in decreased CKX activity in both wt and mutant plants. The treatment increased leaf blade thickness and the volume of chlorophyll-containing cells per unit leaf area in wt but these changes were not observed in the eti5 mutant. The reduction in chlorophyll “a” and “b”, as well as in carotenoids content in the treated wt tissues resulting from altered leaf morphology was not detected in eti5 plants.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

1 Acta Ichthyologica et Piscatoria

1 Acta Neurobiologiae Experimentalis

1 Annales Zoologici

1 Polish Journal of Microbiology

6 Smith A.

2 Alexieva V.

2 Hall M.

2 Karanov E.

2 Todorova D.

2 Vaseva-Gemisheva I.

1 Aalbu R.

1 Bellows C.

1 Genkov T.

1 Genner M. J.

1 Kirejtshuk A.

1 Merkl O.

1 Mzighani S.

1 Nabozhenko M.

1 Ngatunga B. P.

1 Petrov P.

1 Rubia K.

1 Stoynova-Bakalova E.

1 Turner G. F.

1 Varela C.

1 2016

1 2015

1 2014

1 2006