Wyniki wyszukiwania

1

A new thrombolytic, antiplatelet agent with higher fibrin affinity – a staphylokinase variant

100%

Oszajca K.

Acta Biochimica Polonica

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2007

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tom 54

|

nr Supplement 4

2

Udzial tlenku azotu w regulacji ekspresji genow

81%

Oszajca K., Szemraj J., Bartkowiak J.

Postępy Biochemii

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2007

|

tom 53

|

nr 3

254-262

3

Wplyw tlenku azotu na regulacje ekspresji inhibitora aktywatorow plazminogenu typu 1 oraz tkankowego aktywatora plazminogenu

81%

Oszajca K., Szemraj J., Bartkowiak J.

Postępy Biochemii

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2005

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tom 51

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nr 4

407-413

4

The effect of oxidative stress on NADPH oxidase function in human endothelial cells

71%

Kawecka I., Oszajca K., Brown G., Bartkowiak J., Szemraj J.

Acta Biochimica Polonica

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2006

|

tom 53

|

nr Supplement 1

5

Znaczenie bialka MDM2 w cyklu komorkowym

71%

Szemraj J., Rozponczyk E., Bartkowiak J., Greger J., Oszajca K.

Postępy Biochemii

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2005

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tom 51

|

nr 1

44-51

6

The effects of oxidative stress on the expression of PAI-1, t-PA,uPA and uPAR in vascular endothelial cells

61%

Oszajca K., Kowalska I., Bieniasz M., Brown G., Pietras T., Bartkowiak J., Szemraj J. Z.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr Supplement 4

7

Cloning and expression of a new recombinant thrombolytic and antithrombotic agent - a staphylokinase variant

52%

Kowalski M., Brown G., Bieniasz M., Oszajca K., Chabielska E., Pietras T., Szemraj Z., Makandjou-Ola E., Bartkowiak J., Szemraj J.

Acta Biochimica Polonica

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2009

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tom 56

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nr 1

41-53

To develop a more potent antithrombin agent with thrombolytic and antiplatelet properties, a new staphylokinase (SAK) variant was constructed. The kringle 2 domain (K2) of tissue type-plasminogen activator (t-PA) containing a fibrin-specific binding site (i), the RGD sequence (Arg-Gly-Asp) for the prevention of platelet aggregation (ii) and the antithrombotic agent - hirulog (iii) was assembled to the C-terminal part of recombinant staphylokinase (r-SAK). cDNA for the hybrid protein SAK-RGD-K2-Hirul was cloned into Pichia pastoris pPIC9K yeast expression vector. The introduction of K2 t-PA, the RGD sequence and hirulog into the C-terminus of r-SAK did not alter the staphylokinase activity. We observed a higher clot lysis potency of SAK-RGD-K2-Hirul as evidenced by a faster and more profound lysis of 125I-labeled human fibrin clots. The potency of thrombin inhibition by the hirulog C-terminal part of the recombinant fusion protein was almost identical to that of r-Hir alone. These results suggest that the SAK-RGD-K2-Hirul construct can be a more potent and faster-acting thrombolytic agent with better antithrombin and antiplatelet properties compared to r-SAK and SAK-RGD-K2-Hir.

Ograniczanie wyników

4 Acta Biochimica Polonica

3 Postępy Biochemii

7 Oszajca K.

6 Bartkowiak J.

5 Szemraj J.

3 Brown G.

2 Bieniasz M.

2 Pietras T.

1 Chabielska E.

1 Greger J.

1 Kawecka I.

1 Kowalska I.

1 Kowalski M.

1 Makandjou-Ola E.

1 Rozponczyk E.

1 Szemraj J. Z.

1 Szemraj Z.

1 2009

3 2007

1 2006

2 2005

A new thrombolytic, antiplatelet agent with higher fibrin affinity – a staphylokinase variant

Udzial tlenku azotu w regulacji ekspresji genow

Wplyw tlenku azotu na regulacje ekspresji inhibitora aktywatorow plazminogenu typu 1 oraz tkankowego aktywatora plazminogenu

The effect of oxidative stress on NADPH oxidase function in human endothelial cells

Znaczenie bialka MDM2 w cyklu komorkowym

The effects of oxidative stress on the expression of PAI-1, t-PA,uPA and uPAR in vascular endothelial cells

Cloning and expression of a new recombinant thrombolytic and antithrombotic agent - a staphylokinase variant