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1

Research design considerations for deteriorating items of inventory models

100%
Karmveer, Sharma A.
World Scientific News
|
2019
|
tom 124
|
nr 2
2

Localization of nitrogenase and nitrate reductase in the Cycas-Anabaena cycadeae association

81%
Sharma A., Mishra P., Kumar A.
Acta Biologica Cracoviensia. Series Botanica
|
2004
|
tom 46
213-216
Induction of nitrate reductase (NR) activity in coralloid roots of Cycas revoluta was observed after 8 h incubation in 0.02 M KNO3. Other plants growing near Cycas showed a higher level of NR immediately when incubated in KNO3. In contrast to NR, intact coralloid roots showed very high nitrogenase activity (~1.2 to 1.6 µmol C2H4 g fresh wt-1 h-1) under both light and dark conditions as compared to transverse sections of roots. Localization of NR and nitrogenase was tested in coralloid roots using different sets of roots and also in the endophyte. Our results showed that NR activity was mainly due to the endophyte (Anabaena cycadeae); coralloid roots lacked it, as no NR activity was observed in chloramphenicol-treated intact root samples.
3
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Content available

24-epibrassinolide restores the synthesis of proteins and amino acids in Brassica juncea L. leaves under imidacloprid stress

81%
Sharma A., Kumar V., Kumar Thukral A., Bhardwaj R.
Journal of Horticultural Research
|
2017
|
tom 25
|
nr 2
4

Microbial fuel cell: generating electricity from organic waste – a review

81%
Mital S., Srivastava J., Sharma A.
World Scientific News
|
2018
|
tom 113
5

Flow characteristics in a partly vegetated channel with emergent vegetation and seepage

81%
Devi T. B., Sharma A., Kumar B.
International Journal of Ecohydrology and Hydrobiology
|
2019
|
tom 19
|
nr 1
6

Multivariate analysis on the distribution of elements in plants

71%
Kumar V., Sharma A., Bakshi P., Bhardwaj R., Thukral A. K.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 11
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Presence of intracellular viruses in human Cryptosporidium isolates

71%
Sharma P., Khurana S., Sharma A., Sehgal R., Malla N.
Annals of Parasitology
|
2016
|
tom 62
|
nr 2
Cryptosporidium is a major cause of diarrheal illness mainly in children and immunocompromised adults. Disease severity ranges from asymptomatic or self-limited gastroenteritis to acute or chronic diarrhoea which may be associated with systemic features. Intracellular viruses that reside in many parasites have been incriminated in pathogenesis of diseases like trichomoniasis, leishmaniasis etc. Thus we attempted to detect and quantitate the intracellular viruses in Cryptosporidium isolates and sought to seek a relationship if any, with clinical features. Cryptosporidia in stool samples from immunocompromised patients and children were identified by microscopy and species differentiated by PCR-RFLP of 18s rRNA; further subgenotyped by sequencing of GP60 region. Copy number of dsRNA virus and 18srRNA was calculated in 56 Cryptosporidium isolates (39 C. hominis and 17 C. parvum). Viral copy number per oocyst was calculated as ratio of dsRNA virus copy number to 18s rRNA copy number. Viruses were detected in all the isolates. Mean CSpV/RNA ratio was 0.17±0.4 for C. hominis isolates compared with 0.12±0.11 for C. parvum isolates, however this difference was not statistically significant. Similarly no association of diarrhoea, vomiting, cough and fever was found with either CSpV copy number or with CSpV/rRNA ratio.
8
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First report of the earthworm Pontoscolex corethrurus (Müller, 1857) from Punjab, India

71%
Singh S., Singh J., Sharma A., Pal Vig A., Shakoor A.
International Letters of Natural Sciences
|
2018
|
tom 68
The earthworm Pontoscolex corethrurus (Müller, 1857) is reported for the first time from Punjab, India. This species was first described by German naturalist Fritz Müller in 1857 from Santa Catarina state of Brazil. It is commonly found in gardens, cropland and fallow lands. It tolerates wide range of climatic and edaphic factors due to its endogeic ecological category. P. corethrurus has high efficiency for organic matter assimilation and has ability to live in new habitat due to which it can survive even in very poor soil. The life cycle of this species is well documented and this species has economic importance due to its use in waste management.
9

Lipid peroxidation and cell cycle signalling: 4-hydroxynonenal, a key molecule in stress mediated signalling

71%
Yang Y., Sharma R., Sharma A., Awasthi S., Awasthi Y. C.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 2
Role of lipid peroxidation products, particularly 4-hydroxynonenal (4-HNE) in cell cycle signaling is becoming increasingly clear. In this article, recent studies suggest­ing an important role of 4-HNE in stress mediated signaling for apoptosis are criti­cally evaluated. Evidence demonstrating the modulation of UV, oxidative stress, and chemical stress mediated apoptosis by blocking lipid peroxidation by the a-class glutathione S-transferases (GSTs) is presented which suggest an important role of these enzymes in protection against oxidative stress and a role of lipid peroxidation products in stress mediated signaling. Overexpression of 4-HNE metabolizing GSTs (mGSTA4-4, hGSTA4-4, or hGST5.8) protects cells against 4-HNE, oxidative stress (H2O2 or xanthine/xanthine oxidase), and UV-A mediated apoptosis by blocking JNK and caspase activation suggesting a role of 4-HNE in the mechanisms of apoptosis caused by these stress factors. The intracellular concentration of 4-HNE appears to be crucial for the nature of cell cycle signaling and may be a determinant for the signaling for differentiation, proliferation, transformation, or apoptosis. The intracellular concentrations of 4-HNE are regulated through a coordinated action of GSTs (GSTA4-4 and hGST5.8) which conjugate 4-HNE to GSH to form the conjugate (GS-HNE) and the transporter 76 kDa Ral-binding GTPase activating protein (RLIP76), which catalyze ATP-dependent transport of GS-HNE. A mild stress caused by heat, UV-A, or H2O2 with no apparent effect on the cells in culture causes a rapid, transient induction of hGST5.8 and RLIP76. These stress preconditioned cells acquire ability to metabolize and exclude 4-HNE at an accelerated pace and acquire relative resistance to apoptosis by UV and oxidative stress as compared to unconditioned control cells. This resistance of stress preconditioned cells can be abrogated by coating the cells with anti-RLIP76 antibodies which block the transport of GS-HNE. These studies and previous reports discussed in this article strongly suggest a key role of 4-HNE in stress mediated signaling.
10

Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity

61%
Kumar V., Sharma A., Narasimha Prasad B. C., Gururaj H. B., Giridhar P., Ravishankar G. A.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1
Direct shoot bud induction and plant regeneration was achieved in Capsicum frutescens var. KTOC. Aseptically grown seedling explants devoid of roots, apical meristem and cotyledons were inoculated in an inverted position in medium comprising of Murashige and Skoog (Physiol Plant 15:472–497, 1962) basal medium supplemented with 2-(N-morpholine) ethanesulphonic acid buffer along with 2.28 µM indole-3-acetic acid, 10 µM silver nitrate and either of 13.31– 89.77 µM benzyl adenine (BA), 9.29–23.23 µM kinetin, 0.91–9.12 µM zeatin, 2.46–9.84 µM 2-isopentenyl adenine. Profuse shoot bud induction was observed only in explants grown on a media supplemented with BA (26.63 µM) as a cytokinin source and 19.4 ± 4.2 shoot buds per explant was obtained in inverted mode under continuous light. Incorporation of polyamine inhibitors in the culture medium completely inhibited shoothoot bud induction. Incorporation of exogenous polyamines improved the induction of shoot buds under 24 h photoperiod. These buds were elongated in MS medium containing 2.8 µM gibberellic acid. Transfer of these shoots to hormone-free MS medium resulted in rooting and rooted plants were transferred to fields. This protocol can be efficiently used for mass propagation and presumably also for regeneration of genetically transformed C. frutescens.
11

Expression analysis of genes associated with sucrose accumulation in sugarcane under normal and GA3-induced source-sink perturbed conditions

61%
Verma I., Roopendra K., Sharma A., Jain R., Singh R. K., Chandra A.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 06
12

Functional reconstitution of Ral-binding GTPase activating protein, RLIP76, in proteoliposomes catalyzing ATP-dependent transport of glutathione conjugate of 4-hydroxynonenal

61%
Sharma R., Sharma A., Yang Y., Awasthi S., Singhal S. S., Zimniak P., Awasthi Y. C.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 3
Earlier studies from our laboratories have shown that RLIP76, a previously de­scribed Ral-binding GTPase activating protein (Jullien-Flores et al., 1995, J. Biol. Chem. 270: 22473), is identical with the xenobiotic transporter DNP-SG ATPase, and can catalyze ATP-dependent transport of glutathione-conjugates as well as doxorubin (Awasthi et al., 2000, Biochemistry, 39: 9327). We have now reconstituted purified bacterially expressed RLIP76 in proteoliposomes, and have studied ATP-dependent uptake of the glutathione conjugate of 4-hydroxynonenal (GS-HNE) by these vesicles. Results of these studies show that RLIP76 reconstituted in proteoliposomes catalyzes ATP-dependent transport of GS-HNE against a concentration gradient. The transport of GS-HNE is saturable with respect to ATP as well as GS-HNE with Km values of 1.4 mM and 2.5 ,«M, respectively. These studies demonstrate that RLIP76 mediates active transport of GS-HNE, and are consistent with our previous work showing that RLIP76-mediated efflux of GS-HNE regulates the intracellular concentration of 4-HNE and thereby affects 4-HNE mediated signaling.
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