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1

Lesion of the amygdala nuclei in rats determines behaviors diagnostic for autism spectrum disorders

100%
Ptaszek K., Plucinska K., Myslinska D.
Acta Neurobiologiae Experimentalis
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2013
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tom 73
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nr Suppl.1
Autism spectrum disorders (ASD) are a group of pervasive developmental disorder. All of them have been developing from birth, accompanying human through whole life and have strong influence on social functioning, communication as well as cognitive abilities. Neurobiological research (e.g. neuroimaging) indicate that patients with ASD demonstrate impairments of amygdala structure and functioning. It is worth to compare if previous results in human may be observed in animals. The aim of this study was display that lesion of amygdala nuclei may indicate behaviors diagnostic for ASD. For that purpose on a group of male Wistar rats (n=40) was conducted an electrolytic lesion of basolateral (BLA) or centromedial (CeA) amygdala. Animals were divided into groups separately for BLA and CeA: control (without operation), sham (operation, without lesion) and lesioned. After convalescence rats were observed in different behavioral tests which measured social functioning (social interactions), anxiety (elevated plus maze), spatial memory (water maze) and communication (smell preference). Obtain results suggest that amygdala lesion decreased social functioning or anxiety (BLA and CeA), communication (BLA), motor activity (CeA). In spite of this spatial memory increased (BLA). On the base of behavioral results it is likely that lesion of amygdala nuclei may be perceive animal model for further studies. (support: NN303 819040).
2

Brain zif-268 espression under immunization in rats

100%
Myslinska D., Tokarski I., Cecot T., Trojniar W.
Acta Neurobiologiae Experimentalis
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2005
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tom 65
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nr 3
3

Brain c-fos expression after open field stress in rats: influence of social position and responsiveness to novelty

100%
Myslinska D., Lewandowska D., Tokarski J., Trojniar W.
Acta Neurobiologiae Experimentalis
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2003
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tom 63
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nr 5
4

Mild laboratory stress elevates FOS expression in the amygdala and hypothalamus in rats high-responsive to novelty

88%
Majkutewicz I., Myslinska D., Jerzemowska G., Plucinska K., Wrona D.
Acta Neurobiologiae Experimentalis
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2011
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tom 71
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nr S
Responsiveness to novelty is often used as a measure of inter-individual vulnerability to stress loads and drug abuse. The aim of this study was to determine the relationship between individual behavioral profile and brain structures activation. Possible influence of stressful laboratory routines on manifestation of these individual differences was investigated. Male Wistar rats (n=21) were subjected to the novelty test and divided into high (HR) and low (LR) responders to a new environment according to median. Randomly chosen 6 LRs and 5 HRs rats were handled and carried out from the vivarium to the laboratory for nine days (carried group), remaining rats stayed in their home cages (control group, 5 HRs and 5 LRs). One week after the last carrying, an immunohistochemical detection of Fos protein in selected brain areas was performed. Carried HRs showed significantly higher Fos expression in all studied nuclei of the amygdala and most of the hypothalamic areas as compared to LRs and also to control rats. Carried LRs showed elevated density of Fos+ cells only in the stressrelated paraventricular and supraoptic hypothalamic nuclei. Surprisingly, inter-individual (HR vs LR) differences in brain activation was found in carried rats only. We conclude that mild stress evoked by some laboratory routines reveals constitutive differences between the individuals reflected by an increased activity of the amygdala and hypothalamus.
5

Immune effect of electrical stimulation on the bed nucleus of stria terminalis in rats

88%
Myslinska D., Glac W., Ignatowska-Jankowska B., Jankowski M., Trojniar W.
Acta Neurobiologiae Experimentalis
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2007
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tom 67
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nr 3
6

Effects of electrical stimulation of the central nucleus of the amygdala on stress-related behaviors and plasma corticosterone level in rats

76%
Rucinski J., Czerwiec K., Wadolowska A., Kurowska E., Podlacha M., Myslinska D., Jurkowlaniec E.
Acta Neurobiologiae Experimentalis
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2015
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tom 75
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nr Supl.
BACKGROUND AND AIMS: The central nucleus of the amygdala (CeA) has efferent projections to regions involved in autonomic, endocrine, and behavioral responses to emotional stimuli. Due to its role in the regulation of the activity of corticosterone releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus (PVN), the CeA is considered a key modulator of behavioral responses to stress. Examine how electrical stimulation of the CeA, which is known to participate in the integration of sensory and emotional informations with neuroendocrine responses, influences stress-related behaviors and plasma corticosterone level in rats. METHODS: Male Wistar rats implanted under standard stereotaxic surgery with stimulating electrodes into the CeA were divided into the following groups: CeA 14–day electrical stimulation (n=12) and CeA sham (n=10) – control, no stimulation. Behavioral activity during the 5-min testing period in the elevated plus maze (EPM) was recorded and analyzed (e.g. number of entries into closed arms, time spent in open arms). Additionally episodes of grooming were measured. Blood samples were collected by heart puncture (isoflurane anesthesia) seven days before (basal), one hour and seven days after the last stimulation. The plasma corticosterone level (CORT) was determined by radioimmunoassay. RESULTS: Stress-related activity in the EPM and grooming episodes were increased in stimulated rats after last stimulation. Whereas the control group showed gradual adaptation to the testing conditions which was manifested by decreased behavioral activity. Effects of CeA stimulation were also confirmed by an increase in plasma corticosterone level. CONCLUSIONS: Electrical stimulation of the CeA induces stressrelated behavior which is reflected by active avoidance of stressful situation (escape behavior) and adaptive activities to stressful conditions (grooming). Research was supported by The National Science Centre, grant number: NN303819040.
7

Dimethyl fumarate alleviates reference memory impairment and modulates brainderived neurothropic factor expression in streptozotocin-induced rat model Alzheimer's disease

76%
Kurowska E., Majkutewicz I., Myslinska D., Grembecka B., Grzybowska M., Podlacha M., Rucinski J.
Acta Neurobiologiae Experimentalis
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2015
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tom 75
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nr Supl.
BACKGROUND AND AIMS: Intracerebroventricular (icv) injection ofstreptozotocin (STZ) induces brain glucose hypometabolism, memory impairment, progressive cholinergic deficit, activation of microglia, oxidative stress and neurodegeneration. It is used as an animal model of sporadic form of Alzheimer’s disease (AD). The aim of this study was to determine if dimethyl fumarate (DMF), oral anti-oxidative and immunosuppressive drug, alleviates spatial reference memory impairments in STZ-icv induced rat model of AD. Additionally, the expression of brain derived neurotrophic factor (BDNF) was measured. METHODS: There were four experimental groups: STZ DMF (n=8) – STZ-icv infused and fed with 0.4% DMF fodder for three weeks until spatial memory test of Morris, STZ CTR (n=8) – STZ-icv infused and fed with standard fodder, and VEH DMF and VEH CTR groups (n=10) – vehicle-icv infused and fed with 0.4% DMF or standard fodder, respectively. A three-day Morris water maze test (four trials per day with unchanged platform location) and the probe test on the fourth day were performed. Rats were sacrificed and brain subjected to immunofluorescent BDNF labeling. RESULTS: The latency to reach the platform in the second and third day of testing was significantly longer in the STZ CTR rats than in the remaining groups, which showed tendency to reduce the latency day by day. STZ DMF rats did not differ in the results of the spatial memory test of Morris from control VEH CTR and VEH DMF groups. All STZ rats showed reduced BDNF expression in the hippocampus, but in the hypothalamus STZ DMF showed more BDNF+ cells than STZ CTR rats. CONCLUSION: Oral medication with DMF alleviates spatial reference memory impaired after STZ-icv infusion. The decrease of BDNF expression after STZ-icv infusion was prevented by DMF in the hypothalamus. The study was financed by the National Science Centre Poland on the basis of decision DEC-2013/09/D/NZ4/01658.
8

Streptozotocin and dimenthyl fumarate decreases plasma tumor necrosis factor Alpha concentration in rats

76%
Grzybowska M., Podlacha M., Majkutewicz I., Kurowska E., Myslinska D., Grembecka B., Wrona D.
Acta Neurobiologiae Experimentalis
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2015
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tom 75
|
nr Supl.
BACKGROUND AND AIMS: This study aims to determine if dimethyl fumarate (DMF), antioxidant having immunosuppressive properties, taken orally for three weeks will affect plasma tumor necrosis factor  alpha (TNF-α) concentration in an animal model of sporadic form of Alzheimer’s disease (sAD) induced by intracerebroventricular (icv) injection of betacytotoxic drug, streptozotocin (STZ). METHODS: Blood samples from young, male Wistar rats divided into four groups: STZ DMF (subjected to icv injection of STZ, fed with 0.4% DMF fodder), VEH DMF (subjected to icv injection of vehicle, fed with 0.4% DMF fodder), STZ CTR (subjected to icv injection of STZ, fed with standard fodder), VEH CTR (subjected to icv injection of vehicle, fed with standard fodder) were taken one hour after Morris water maze test finishing. Then, TNF-α concentration was determined with ELISA method using a Rat TNF-α ELISA Kit. RESULTS: Injections of STZ in rats being on the control feed (STZ/ CTR) significantly decreased (P≤0.05) the  plasma concentration of  TNF-α (22±2 pg/ml; mean±SE) as compared to the controls (VEH/CTR: 33±3 pg/ml). Moreover, within the STZ/DMF group, a significant (P≤0.01) decrease in the concentration of  TNF-α (22±0.8 pg/ml)  as compared to the controls  (VEH/DMF: 30±2 pg/ ml), was observed. CONCLUSION: The obtained results indicate that streptozotocin injection and feeding with dimethyl fumarate of the streptozotocininduced sAD rats reduce such a peripheral blood pro-inflammatory cytokine level as TNF-α.
9

Dimethyl fumarate prevents spatial working memory impairment and does not affect brain IL-6 expression in streptozotocininducted rat model of Alzheimer's disease

76%
Majkutewicz I., Kurowska E., Myslinska D., Grembecka B., Grzybowska M., Podlacha M., Rucinski J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Animal model of sporadic form of Alzheimer Disease (AD) evoked by intracerebroventricular (icv) injection of betacytotoxic drug, streptozotocin (STZ), reflects memory impairments, brain hypometabolism, cholinergic deficit, activation of microglia and neurodegeneration found in AD patients. Brain inflammation is important factor contributing to exacerbation of AD symptoms, but some studies show neuroprotective properties of pro-inflammatory cytokine IL-6. The aim of this study was to determine if dimethyl fumarate (DMF), which has anti-oxidative and immunosuppressive properties, can alleviate spatial working memory impairments in STZ-icv induced rat model of AD and change the expression of IL-6 in the brains. METHODS: Four experimental groups were separated: STZ DMF (n=8) – STZ-icv infused and fed with 0.4% DMF fodder for three weeks until spatial memory test of Morris, STZ CTR (n=8) – STZicv infused and fed with standard fodder, and VEH DMF and VEH CTR groups (n=10) – vehicle-icv infused and fed with 0.4% DMF or standard fodder, respectively. Morris water maze testing was performed for three days, with four trials per day with unchanged platform location, and rats were then sacrificed and brains subjected to immunofluorescent IL-6 labeling. RESULTS: The latency to reach the platform in each trial was significantly longer in the STZ CTR rats than in the remaining groups. STZ CTR was the only group which did not decrease the latency and the distance swum to platform in the consecutive trials. STZicv infused rats  (STZ CTR and STZ DMF groups) had also lower number of the IL-6 expressing cells in the hippocampus and the hypothalamus than control VEH CTR rats. CONCLUSION: Oral medication with DMF prevents spatial working memory impairment evoked by STZ-icv infusion, but has no influence on the central expression of IL-6. The study was financed by the National Science Centre Poland on the basis of decision DEC-2013/09/D/NZ4/01658.
10

Lesion and stimulation of the mesolimbic motivational dopamine system influence blood peripheral lymphocyte percentage in rats differing in locomotor activity

76%
Plucinska K., Grembecka B., Glac W., Myslinska D., Jerzemowska G., Wrona D.
Acta Neurobiologiae Experimentalis
|
2011
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tom 71
|
nr S
The mesolimbic dopamine system, that plays a pivotal role in emotionally motivated behaviours, originates in the ventral tegmental area (VTA) and sends major projections to the nucleus accumbens (Acb). In the present work, we assessed the effects of manipulation in the mesolimbic dopaminergic system identified with the “brain reward system” on the percentage of peripheral blood lymphocytes in freely moving rats differing in locomotor activity to novelty (high responders; HR or low responders; LR). T and B lymphocyte populations were determined by flow cytometry (CD3-FITC/CD45RA-PC7). Peripheral blood was collected from rats exposed to chronic 14 day VTA electrical stimulation to obtain feeding or exploration response (constant current 0.1 ms duration, cathodal pulses, 50 Hz, 30-min daily session) and with a lesion electrode aimed at Acb: 3 weeks after electrode implantation, after 2 weeks of chronic VTA stimulation, on the 2nd day after the Acb lesion (2mA for 15s) and on the 14th VTA stimulation day following the Acb lesion. As compared to the respective sham animals, chronic stimulation of the VTA caused significant (p<0.05) decrease in T cell percentage in both HR (33.32 ± 5.39% vs 48.04 ± 7.45%) and LR (44.99 ± 2.94% vs 51 ± 3.57%) animals. In addition, significantly (p<0.05) lower level of T cell percentage in HR animals (40.13±3.64% vs 50.94 ± 5.87%) rats were observed. On the 14th VTA stimulation day following the Acb lesion, significant (p<0.05) decrease in B cell percentage in both HR (12.47 ± 3.55% vs 19.45 ± 1.58%) and LR (7.09 ± 2.48% vs 16.5 ± 3.46%) animals was observed. On the other hand, chronic stimulation of the VTA (20.99 ± 3.38% vs 17.65 ± 0.49%) and Acb lesion (25.14 ± 2.48% vs 20.62 ± 0.85%) caused significant increase (p<0.05) in B cell percentage in HR animals. The results emphasize the importance of individual differences (HR vs LR) in the influence of the mesolimbic motivational system on blood lymphocyte distribution.
11

Genistein-mediated lysosomal degradation of main patogenic factors of Alzheimer’s disease as a novel therapeutic strategy

76%
Pierzynowska K., Podlacha M., Gaffke L., Majkutewicz I., Mantej J., Myslinska D., Wegrzyn G.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
12

Brain-derived neurotropic factor (RDNF) expression and behavioral response during stimulation of bed nucleus of the stria terminalis (BST) in rats

64%
Myslinska D., Majkutewicz I., Podlacha M., Ciepielewski Z., Karnia M., Wadolowska A., Rucinski J., Siudak M., Zajaczkowski S., Wrona D.
Acta Neurobiologiae Experimentalis
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2015
|
tom 75
|
nr Supl.
BACKGROUNDANDAIMS: The bed nucleus of the stria terminalis (BST) is a part of “the extended amygdala”, a formation responsible for emotional aspects of behavior. The BST is considered as a site of convergence of information from brain regions associated with the control of emotional, cognitive, autonomic and behavioral responsesto stress and noxiousstimuli. On the basis of our previousstudy we assumed that the BST also influenced the primary antitumor immune response. In the present study we investigated the influence of 14-day electrical stimulation of the BST on the brain-derived neurotrophic factor (BDNF) expression and behavioral response. METHODS: Male Wistar rats implanted with electrodes into the BST were divided into groups: BST stimulated and BST sham. The intensity of stimulation current (120–160 µA; 50 Hz) was determined individually for each stimulated rat to induce a behavioral response such a locomotor reaction. The current intensity was raised incrementally in 30 s trials (30 trials/day, 20 s rest between the trials). Behavioral reaction was measured in the Opto Varimex Minor actometer during stimulation procedure. BDNF was detected during immunofluorescence procedure. RESULTS: The stimulation of the BST caused induction of BDNF expression in brain cortical and subcortical motor structures: the frontal primary motor cortex (areas FR1 and FR2), prefrontal cortex, ventral tagmental area, as well as in the central amygdala nucleus and in the hypothalamus: the paraventricular and the supraoptic nuclei, the medial preoptic and the lateral areas positively correlated with the augmentation of the behavioral activity which appeared as locomotor activity (increase in the average number of movements in horizontal and vertical plane). CONCLUSION: This suggests that the behavioral outcome of the BST stimulation, imitating physical exercise, could be responsible for brain BDNF synthesis observed in the study. Supported by: NN303819040.
13

Behavioral responses to amygdaloid stimulation

64%
Myslinska D., Rocinski J., Czerwiec K., Wadolowska A., Ciepielewski Z., Kurowska E., Majkutewicz L., Plucinska K., Podlacha M., Ptaszek K.
Acta Neurobiologiae Experimentalis
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2014
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tom 74
|
nr 3
The central nucleus of the amygdala (CeA) is involved in fear conditioning and participates in euroendocrine response to stressful stimuli. In our previous study, we found that CeA also influences immune function, especially innate antitumore response. In the present study, we investigated the influence of 14-day electrical stimulation of the CeA on corticosterone level (CORT; determined by radioimmunoassay) and behavioral response: locomotor activity in the new environment (measured in the Opto Varimex Minor actometer), explorative and anxiety-like behavior [assessed in the openfield (OF) and in the elevated plus-maze (EPM) tests]. Male Wistar rats implanted with stimulating electrodes into the CeA were divided into groups: CeA 14-day electrical stimulation (n=20) and CeA sham (n=18). Current intensity (70–120 µA; 50 Hz) was raised incrementally in 30-s trials until behavioral reaction in the Opto Varimex Minor actometer was observed. OF and EPM tests were performed after termination of stimuation procedure. Electrical stimulation of the CeA caused augmentation of CORT level (P<0.001) correlated with an increase in the average number of movements in horizontal and vertical plane imitating escape behavior. These effects were accompanied by an increase in anxiety-related behavior in the EPM and decreased locomotor activity and exploration in the OF. We suggest the hormonal response to of the CeA stimulation plays a crucial role in the regulation of the behavioral response. Supported by The National Science Centre, grant number: NN303819040.
14

Electrical stimulation of the medial septal nucleus increases cytotoxic activity of NK cells in the spleen and peripheral blood in rats

64%
Plucinska K., Grembecka B., Glac W., Budtke P., Jerzemowska G., Myslinska D., Orlikowska A., Wrona D.
Acta Neurobiologiae Experimentalis
|
2009
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tom 69
|
nr 3
The medial septal nucleus (MS) is a forebrain limbic structure involved in learning and memory mechanisms. In previous study we found that electrolytic lesion of the medial septal nucleus caused depression of the peripheral blood natural killer cell cytotoxicity (NKCC) and the leukocyte number. In the sham operated group mere insertion of electrodes into the MS evoked transient NKCC enhancement, probably resulting from mechanical MS stimulation. To check this effect in the present study, we evaluated both spleen and blood NKCC (51Cr-release assay) and large granular lymphocytes (LGL) number (a morphological method) after chronic electrical stimulation (constant current 0.1 ms duration cathodal pulses delivered at a frequency of 50 Hz during 30-min daily session for 14 consecutive days) of MS in conscious, freely moving rats (n=12). Additionally, peripheral blood leukocyte, lymphocyte and neutrophile number was mesaured. Chronic MS stimulation caused signifi cant blood NKCC augmentation and LGL number (25.86 ± 9.31% vs. 15.75 ± 4.75%, P<0.01) in comparison to the sham group (n=13). No signifi cant effect was found in the spleen (27.14 ± 9.99% vs. 28.58 ± 8.04%). A week after termination of the stimulation procedure all measured parameters returned to the baseline. The results obtained indicate that such limbic structure as medial septum enhances antitumor and antiviral function and number of NK cells.
15

Stimulation of the bed nucleus of the stria terminalis and the medial septal nucleus influences natural killer cell cytotoxicity of rats differing in responsiveness to novelty

64%
Myslinska D., Plucinska K., Glac W., Grembecka B., Listowska M., Podlacha M., Ptaszek K., Wrona D.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
In our previous study we found that electrolytic lesion of the bed nucleus of the stria terminalis (BST) as well as the medial septal nucleus (MS) caused depression of the peripheral blood natural killer cell cytotoxicity (NKCC) in rats. In the present study we evaluated blood NKCC after 14 day electrical stimulation of the BST and the MS in conscious, freely behaving rats differing in responsiveness to novelty. Male Wistar rats divided into high (HR) and low (LR) responders to novelty, implanted with stimulating electrodes at the BST or at the MS, were subjected to 14 day electrical stimulation (constant current 0.1 ms duration cathodal pulses delivered at a frequency of 50 Hz during 30 min) of the BST and the MS. The chronic stimulation of the BST and the MS caused augmentation of blood NKCC in comparison to the sham operated group and to the baseline, which was more significant in HRs. A week after termination of the stimulation procedure NKCC returned to the baseline. The obtained results suggest that immunoenhancing effect on blood NK cell function is dependent on the behavioral outcome (intensive locomotor reaction) of the BST and the MS stimulation as well as on individual behavioral characteristics. This work was supported by a research grant NN303819040.
16

Proces autofagii indukowany genisteiną jako nowe podejście w leczeniu chorób neurodegeneracyjnych

64%
Pierzynowka K., Podlacha M., Myslinska D., Majkutewicz I., Mantej J., Niedzialek N., Wegrzyn A., Wegrzyn G.
Postępy Mikrobiologii. Suplement
|
2017
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tom 56
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nr 2
17

Functional reorganization of the mesolimbic system after its terminal area destruction influences blood leukocytes and their subsets

64%
Plucinska K., Grembecka B., Myslinska D., Listowska M., Glac W., Jerzemowska G., Badtke P., Wrona D.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
Contralateral nucleus accumbens shell (AcbS) lesions (Contra group) impaired (by about 20%) and ipsilateral AcbS lesions (Ipsi group) facilitated (by about 30%) motivational aspects of ventral tegmental area (VTA) stimulation-induced feeding or exploration which manifested as respective alterations in latency to reaction. Present work was aimed to examine how this motivational reorganization of AcbSVTA circuitry affect on blood leukocytes and their subsets (morphological method). As compared to the respective sham animals, the chronic VTA stimulation and unilateral lesion of the AcbS caused a significant decrease in total leukocyte and lymphocyte numbers in Ipsi and Contra groups. Both groups showed also significant decreases in total leukocyte and lymphocyte numbers on the 2nd day after unilateral lesion of the AcbS. On the 14th VTA stimulation day following unilateral lesion of the AcbS total leukocyte and a large granular lymphocyte (LGL) number was higher in Ipsi group in relation to Contra group and in comparison with the respective sham group. Increased motivational drive associated with facilitation reactivity of the ipsilateral VTA to lesioning AcbS enhance total leukocyte number, especially LGL cells that are critical to the innate immune system.
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