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1

Atypowe pestiwirusy - ryzyko dla hodowli bydła i znaczenie dla kontroli wirusowej biegunki bydła i znaczenie dla kontroli wirusowej biegunki bydła i choroby błon śluzowych (BVD-MD)

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Kuta A., Larska M., Polak M. P., Zmudzinski J. F.
Medycyna Weterynaryjna
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2012
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tom 68
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nr 02
The paper presents the current state of knowledge on the prevalence and risk of a new type of bovine viral diarrhea virus known as type 3 (BVDV-3). The first discovered atypical pestivirus was the isolate D32/00_’HOBI’, detected in fetal calf serum (FCS) originating from Brazil. The isolates CH-KaHO/cont, SVA/cont-08 and IZSPLV_To are further examples confirming the presence of BVDV type 3 in the FCS. This new species of pestiviruses (BVDV-3) is a problem not only for research laboratories using bovine serum, but also for cattle breeders. Natural infections with this virus have been reported in Brazil, Thailand and Italy, which may suggest that the new species of pestivirus is also present in European cattle. The methods used for routine diagnosis of BVDV infection are ineffective in detecting atypical pestiviruses, which may pose a risk of false negative results. It may also influence the safety of vaccines and biological products produced on the basis of contaminated batches of fetal bovine serum.
2

Monitoring of bovine viral diarrhoea virus (Bvdv) infection in polish dairy herds using bulk tank milk samples

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Kuta A., Polak M. P., Larska M., Zmudzinski J. F.
Bulletin of the Veterinary Institute in Puławy
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2013
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tom 57
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nr 2
The aim of the study was to evaluate the status of bovine viral diarrhoea virus (BVDV) infection in selected dairy herds in Poland with the use of commercial enzyme linked immonosorbent assay for the detection of specific antibodies (BVDV-Ab ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) for the detection of viral RNA, using bulk tank milk (BTM) samples. Two hundred and thirty-one samples of BTM were collected from 99 dairy herds in Poland. The herds were divided into four different classes according to the Swedish system of classification. The results showed that 70.7% of herds were BVDV antibody- positive. High levels of antibodies in 52.85 % (37 herds in class 3) of all antibody positive herds indicated acute BVDV infection. Thirty five samples with the highest antibody levels were tested by RT-PCR and five of them were positive for viral RNA. Dairy herds in Poland have high levels of antibodies against BVDV in BTM. Since no vaccination was implemented in the herds tested, high seroprevalence of BVDV antibodies in cattle indicates the widespread of BVDV infection in Polish cattle.
3

Genetic typing of bovine viral diarrhoea virus (BVDV) by restriction fragment length polymorphism (RFLP) and identification of a new subtype in Poland

100%
Kuta A., Polak M. P., Larska M., Zmudzinski J. F.
Bulletin of the Veterinary Institute in Puławy
|
2015
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tom 59
|
nr 1
Restriction fragment length polymorphism (RFLP) analysis was developed for genetic ty ping of Polish strains of bovine viral diarrhoea virus (BVDV). The method was applied using 60 BVDV isolates, which included BVDV genotype 1, subtypes a, b, d, e, f, and g, and genotype 2a. RT-PCR products of the 5'untranslated region (5'UTR) were digested using three enzymes. Restriction patterns classified the strains into seven groups, each with a specific and different pattern from other subty pes. These findings were confirmed by nucleotide sequencing and phylogenetic analysis. The results suggest that RFLP analysis is a simple, reliable, and fast genotyping method for BVDV strains in comparison with sequencing. This method can distinguish six subtypes of BVDV-1 including a new subtype 1e, identified exclusively by this method, and it allows differentiation of BVDV-1 from BVDV-2 genotype.
4

Evaluation of diagnostic methods to distinguish between calves persistently and transiently infected with bovine viral diarrhoea virus in respect to the presence of maternal antibodies

100%
Larska M., Kuta A., Polak M. P.
Bulletin of the Veterinary Institute in Puławy
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2013
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tom 57
|
nr 3
Two issues concerning virus detection and identification of persistently infected (PI) cattle were analysed in the study: 1) interference by maternal antibodies and 2) discrimination between PI and transiently infected (TI) animals. Antigen ELISA and RT-PCR based methods were compared using serum samples from natural and experimental PI and TI calves. RT-PCR and realtime RT-PCR using primers within 5'UTR region were more sensitive in detecting PI animals than Ems and NS3 antigen capture ELISAs, and they were not influenced by the presence of colostral antibodies in serum or by bovine viral diarrhoea virus genotype. The serum samples with Ct values ≤ 29.10 (corresponding to 104.87 viral RNA copies/µL) identified PI animals with 100% probability, while all samples with Ct values > 32.06 (corresponding to viral RNA load below 10⁴ copies/µL) indicated TI status. The samples with Ct values between 29.10 and 32.06 (17.2% of PI and 11.5% of TI) should be considered as PI suspect and retested.
5

Budowa herpeswirusa bydła typu 1 i patogeneza zakażeń tym wirusem

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Adaszek L., Kuta A., Urban-Chmiel R., Winiarczyk S.
Życie Weterynaryjne
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2010
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tom 85
|
nr 09
6

Atypowe pestiwirusy - nowe wyzwanie dla diagnostyki i kontroli zakażeń bydła wirusem BVD-MD

100%
Polak M., Kuta A., Larska M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
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2014
|
tom 09
|
nr 4
7

Spread of Schmallenberg virus infections in the ruminants in Poland between 2012 and 2013

100%
Larska M., Kesik-Maliszewska J., Kuta A.
Bulletin of the Veterinary Institute in Puławy
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2014
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tom 58
|
nr 2
The aim of the study was to evaluate the prevalence of Schmallenberg virus (SBV) specific antibodies in ruminants from 13 Polish provinces, sampled between January 2010 and August 2013. A total number of 1813 serum samples from cattle, sheep, goats, mouflon, wild and farmed cervids, and European bison were tested by ELISA for viral nucleoprotein antibodies. First SBV seropositive animals were identified in August 2012 (1.6%), and the percentage increased gradually, reaching 57.1% in December of this year. The proportion of seropositive animals in 2013 at the level of 34.2% increased tenfold in comparison to 2012 (3.4%), which was particularly significant at the level of individual provinces. In 2013, the highest percentages of SBV seropositive animals were found in Dolnoslaskie (92.3%), Podlaskie (82.3%), and Zachodniopomorskie (80.9%) provinces. Significant associations between the seroprevalence and province of origin, month, ruminant species, and insect vector activity were found, while no dependence of animal age on seropositivity was observed. The differences between SBV seropositive large and small ruminants suggested the involvement of some vector exposure factors, which needs to be further investigated.
8

Realizacja i efekty ekonomiczne programu zwalczania zakażenia BVD-MD na przykładzie gospodarstwa bydła mlecznego

76%
Polak M. P., Manka P., Kubasik D., Rola J., Kuta A., Larska M., Zmudzinski J. F.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2013
|
tom 08
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nr 1
9

Analiza molekularna fragmentu genu glikoproteiny gC wirusa BHV-1 izolowanego od cieląt z terenów wschodniej Polski

76%
Adaszek L., Kuta A., Kalinowski M., Urban-Chmiel R., Rola J., Zietek J., Winiarczyk S.
Medycyna Weterynaryjna
|
2011
|
tom 67
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nr 05
10
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Obraz kliniczny zakażeń wirusem Schmallenberg

76%
Larska M., Tarkowska K., Kuta A., Fidler-Kwiatek E., Ciastek M., Zmudzinski J. F.
Życie Weterynaryjne
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2013
|
tom 88
|
nr 06
11

Status wybranych chorób wirusowych i BSE w populacji bydła w Polsce

64%
Zmudzinski J. F., Rola J., Polak M. P., Larska M., Smreczak M., Orlowska A., Trebas P., Kuta A., Kesik-Maliszewska J.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2015
|
tom 10
|
nr 2 Monografia
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