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This report starts with an overview based on national-level data from official sources and then presents the findings of a survey of rural households conducted in 2001 in Mazowieckie region, Poland. The study focuses on income distribution differences and income level comparison among four subregions. The change of rural household living standard during the socio-economic transformation and the attitudes of rural households towards the transformation are also examined. By analyzing source of income and related effect factors, some components of potential sources of income and the policy urgently needed to develop rural area in Poland are suggested.
In this study, the effects of salicylic acid to antioxidative activity and photosynthetic characteristics in waterlogging stress of two peony cultivars (‘Fengdanbai’ and ‘Mingxing’) were investigated. 4-year-old peony grown in different levels of waterlogging stress and then different concentration prepared SA (0.0, 0.1, 0.5 and 1.0 mmol L⁻¹) sprayed on fresh leaves of peony. The antioxidative enzymes activities include superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), chlorophyll content, relative conductivity and MDA content were measured in leaves about different waterlogging treatment, the photosynthetic characteristics were also measured using photosynthetic measurement system. The results showed that waterlogging stress decreased the chlorophyll content in all peony cultivars leaves, but with SA treatment can inhibit the decrease of chlorophyll content. Relative conductivity increased as the extension of waterlogging time in two cultivars. SA treatment could effectively inhibit the increase of relative conductivity, and 0.5 mmol L⁻¹ of SA was the most suitable concentration. SOD, POD, CAT activity increased first and then decreased in different waterlogging condition, SA significantly increased the activity of various enzymes. MDA content was increase as the expansion of waterlogging time in two cultivars. SA inhibits the increase of MDA content. Of all concentration of SA, 0.5 mmol L⁻¹ was the best concentration to inhibit the waterlogging stress. For the photosynthetic characteristics, the net assimilation rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) and intercellular CO₂ (Ci) were decreased under different waterlogging condition. SA treatment can increase Pn, Gs, Tr and Ci of peony.
Taking small-scale areas as research units, this paper empirically explores the dynamic relationship between economic development and carbon emissions from 1995 to 2013 in China’s low-carbon pilot provinces. This subject has been neglected in the literature. We first apply decoupling theory to analyse the decoupling of economic development and carbon emissions. We then apply a STIRPAT model and ridge regression to deconstruct the mechanism whereby economic development influences carbon emissions in China’s low-carbon provinces. Empirical results show a positive linear correlation between economic development and carbon emissions in the examined provinces. We find that population size and industrial structure most prominently affect carbon emissions; however, per capita wealth, technological progress, and policy factors do not show the expected significance. Our results allow policymakers to formulate lowcarbon policies on the basis of empirically verified situations in the examined regions. They suggest that restricting population growth and transforming industrial structures are the keys to reducing China’s carbon emissions.
There is an apparent allometric relationship between peak frequency of echolocation and body size in rhinophilids. However, some rhinolophids deviate from this rule. To date this variation has been explained as a result of partitioning of communication channels. An alternative hypothesis that food resource partitioning results in this divergence in expected frequencies was tested by comparing prey selection between Rhinolophus macrotis Blyth, 1844 and Rhinolophus lepidus Blyth, 1844 in Yunnan Province, China. These two sympatric species are morphologically similar but acoustically divergent: R. macrotis has an echolocation frequency significantly lower than that predicted by the allometric relationship, whereas that of R. lepidus agreed with expectations. Prey selection experiments, conducted in a flight tent, indicated that the dominant prey taxa of R. macrotis were Lasiocampidae, Arctiidae and Noctuidae, whilst that of R. lepidus were Arctiidae, Noctuidae and Ichneumonidae. R. macrotis ate more earless moths and fewer eared moths than R. lepidus, and R. macrotis fed on larger prey in general and captured a wider size range than that captured by R. lepidus. These results confirmed the existence of finely tuned trophic niche differentiation and suggested that food resource partitioning is one of the factors leading to lower peak frequency of calls in R. macrotis.
Atrazine is a photosystem-II-inhibiting herbicide that interferes with photosynthetic electron transport, resulting in oxidative stress. Soybean (Glycine max (L.) Merrill) is an atrazine-sensitive crop, and its productivity is severely impacted by soils containing atrazine residues. Our previous study indicated that the bacteria Klebsiella pneumoniae strain SnebYK-induced resistance to atrazine in soybean, both before and after pasteurization. In order to study the molecular mechanisms of this induced resistance, proteins change in soybean leaves induced by SnebYK was investigated using two-dimensional gel electrophoresis. Differentially expressed proteins (relative to a non-induced control) were identified using MALDI-TOF MS. Differential expression patterns were detected in soybean leaves that had been induced by the bacterium. Analysis of relative expression levels indicated up-regulation of most of the mRNAs in these samples relative to the control. The corresponding proteins were observed to be involved primarily in physiological processes, including active oxygen removal, resistance signal transduction, and photosynthesis. This is the first study to conduct proteomic analysis of a soybean resistance response induced by bacteria. It is plausible that these differentially expressed proteins may interact to play a major role in defense and/or resistance responses.
Distribution pattern of δ¹³C values of plateau plants and their responses to environment along altitudinal gradients were investigated. In the growing season of 2003 (June– August), stable carbon isotope ratios (δ¹³C) of 174 plant samples belonging to 89 species of 20 families and 58 genera along the gradient 2800– 4400 m (above sea level) was studied in six sites on the east edge of Qinghai-Tibet Plateau. The results indicated that the range of δ¹³C values of plants is narrow from –30.2‰ to –25.2‰, which means that none of the species examined belonged to C₄ photosynthetic pathway and all of these species performed C₃ photosynthetic pathway. The average δ¹³C values of plants at 6 sites were positively correlated to altitude (r = 0.974, P <0.01). The results revealed that site-averaged δ¹³C values were negatively correlated with temperature (r = 0.907, P <0.05) as well as CO₂ partial pressure (r = 0.940, P <0.01). The combination of these two factors account for 80% of the variation of δ¹³C values (r² = 0.859, P <0.01). Varying precipitation with increasing altitude does not affect the plant δ¹³C values (r = 0.469, P> 0.05) as well as the sunlight duration(r = 0.630, P> 0.05).
Hes l, a hairy and enhancer of split homolog, negatively regulates inner ear hair cell differentiation. The main objective of this study was to investigate the status of the Hes l gene in the noise-damaged cochlea in relation to the degree of noise-induced hearing loss (NIHL). Adult albino guinea pigs were exposed to white-band noise (115 dB sound pressure level). Noise exposure for either 1 or hours induced significant elevations of threshold in auditory brainstem response (ABR) compared with unexposed controls. Succinate dehydrogenase staining showed that white-band noise exposure caused significant outer hair cell losses. In addition, we found significant up-regulations of cochlear Hesl mRNA and protein expressions following acoustic trauma, and Hesl mRNA expression was positively correlated with NIHL. These findings suggest that up-regulation of Hes l expression in response to noise exposure may be one of the underlying mechanisms of NIHL.
Arbuscular mycorrhizal (AM) symbiosis can improve the growth of poplar, which is considered to be a bioenergy feedstock and a substitute for fossil fuels. Microbes in plant rhizosphere may affect the interaction between AM fungi and host plant. The role of AM fungi on the biomass and bioenergy production of poplar in the presence or absence of other soil microorganisms was less investigated. In this study, the effects of Rhizophagus irregularis and Glomus versiforme on the growth, root-absorbing area, chlorophyll content, gas exchange, total organic carbon (TOC) content, gross calorie value (GCV), lignin and cellulose content of Populus × canadensis ‘Neva’ in sterilized and unsterilized soil were evaluated. The results showed that AM symbiosis increased the biomass parameters such as stem length, ground diameter, dry weight, root-absorbing area, chlorophyll content and gas exchange capacity, as well as bioenergy parameters such as TOC content, GCV and lignin content of poplar seedlings, but did not affect the cellulose content. The extent of this enhancement varied within AM fungi species and soil conditions. The effect of AM was higher in sterilized soil than that in unsterilized soil, and the function of R. irregularis was greater than G. versiforme in sterilized soil, but lower in unsterilized soil. In general, G. versiforme exhibited higher applying potential in the biomass and bioenergy production of poplar.
Silicon (Si) has been verified to play an important role in enhancing plant resistance against pathogens, but the exact mechanisms remain unclear. Two near-isogenic lines of rice (Oryza sativa L.), CO39 (blast susceptible), and C101LAC (Pi-1) (blast resistant), were hydroponically grown to study the effects of exogenous silicon application on the changes of disease incidence, mineral nutrient concentrations, chlorophyll content, and photochemical efficiency in Magnaporthe oryzae infected rice plants. Si amendment in nutrient solution at a concentration of 2.0 mM significantly reduced the disease index of rice plants of CO39 and C101LAC (Pi-1). Silicon application alone had no effects on mineral nutrient contents, chlorophyll content, maximum/potential quantum efficiency (Fv/Fm), and the maximum primary yield (Fv/F0) of photochemistry of PS II in healthy rice leaves. M. oryzae inoculation significantly increased the content of K, Na, Ca, Mg, Fe, and reduced the value of Fv/F0 and Fv/Fm in rice leaves. However, Si treatment suppressed M. oryzae induced increase of mineral nutrient contents, and significantly increased Fv/F0 and Fv/Fm value compared with Sideficient infected plants. These results suggest that siliconenhanced resistance to rice blast is associated with an enhancement of photochemical efficiency and adjustment of mineral nutrient absorption in M. oryzae-infected rice plants.
Dwarfism was one of the most important agronomic traits in cucumber breeding. The current study was conducted to identify dwarf-related proteins using twodimensional electrophoresis. Twenty-two differentially expressed protein spots were detected between dwarf and vine genotypes while 20 of them were successfully identified by MALDI-TOF/TOF MS. Out of 20 identified proteins in dwarf genotype, 14 were up-regulated and six were down-regulated. The classification of differential proteins showed that the identified proteins were functionally involved in photosynthesis, energy metabolism, cytoskeletal functions, transduction and signal regulation, detoxification and redox regulation. Five differentially regulated proteins were analyzed using the technique quantitative real-time PCR (qRT-PCR). The results showed that four proteins, including histone deacetylase, the EIN2- like protein, chlorophyll A/B binding protein, and ubiquitin-conjugating enzyme Spm2 (E2) were up-regulated and one F-box family protein was down-regulated in the dwarf genotype. Further western blot analysis revealed that only E2 protein was detected in the dwarf genotype, indicating its important role in the cucumber dwarf trait.
hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.
In this study, wastes and minerals were incorporated into the production of bricks as a kind of waterpurifying substrate. The brick contained 72% clay, 10% bagasse, 10% steel slag, 5% zeolite, and 3% calcite by weight. The brick exhibited highly and hierarchically porous structure. It can remove phosphate and ammonium simultaneously from aqueous solutions with the maximum adsorption amount of 3.8 mg/g for phosphate and 0.7 mg/g for ammonium. Kinetic analysis showed that the brick had a faster removal rate for phosphate than ammonium. The maximum desorbability of phosphate and ammonium were found to be 5.9% and 13.9%, respectively. Considering wastes recycling, easy fabrication, and favorable properties, this type of brick has the potential to be applied to in-situ remediation of aquatic environment.
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