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Bacterial community DNA extracted from soils polluted with heavy metals

100%

Kozdroj J., van Elsas J. D.

Polish Journal of Environmental Studies

2000

tom 09

nr 5

403-407

This paper describes the application of two direct and one indirect methods for the extraction of microbial community DNA from soils polluted with heavy metals. DNA was extracted directly from soil by a gentle method based on the soil incubation at 37°C with proteinase K and SDS or the method was modified by the addition of bead beating step. The indirect approach was based on the RNA/DNA extraction method. The level of soil contamination did not affect on the yields of DNA extracted and PCR amplification of the target DNA. The results indicated that the DNA obtained by the applied protocols was sufficiently pure for further molecular analyses.

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Peanut allergy as a tigger for the deterioration of atopic dermatitis and precursor of staphylococcal and herpetic associated infections - case report

41%

de Carvalho Ferreira D., Abad E. D., Cavalcante F. S., dos Santos F. M., Saintive S., Goudoris E., do Prado E. A., Ribeiro M., Rosado A. S., van Elsas J. D., dos Santos K. R. N.

Annals of Agricultural and Environmental Medicine

2015

tom 22

nr 3

Ograniczanie wyników

1 Annals of Agricultural and Environmental Medicine

1 Polish Journal of Environmental Studies

2 van Elsas J. D.

1 Abad E. D.

1 Cavalcante F. S.

1 Goudoris E.

1 Kozdroj J.

1 Ribeiro M.

1 Rosado A. S.

1 Saintive S.

1 de Carvalho Ferreira D.

1 do Prado E. A.

1 dos Santos F. M.

1 dos Santos K. R. N.

1 2015

1 2000

Wyniki wyszukiwania

Bacterial community DNA extracted from soils polluted with heavy metals

Peanut allergy as a tigger for the deterioration of atopic dermatitis and precursor of staphylococcal and herpetic associated infections - case report