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The purpose of the study was to evaluate the usefulness of magnetic resonance (MR) in imaging of the cisternal and petroclival segments of the abducent nerve. Heavily T2-weighted submillimetric 3D sequence in axial plane, T1-weighted 3D, 1.5 mm slice thickness sequence in axial plane and TOF sequence were performed on 16 volunteers. Additionally the reformatted T2-weighted images in sagittal and in oblique parasagittal plane parallel to the abducent nerve in the pontine cistern were performed. The heavily T2-weighted sequence provides high contrast between fluid and other structures. High signal intensity of the cerebrospinal fluid is a kind of background for cranial nerves, vessels, meninges as well as for bony and fibrous structures. The authors identified the abducent nerve in at least one plane of the submillimetric, heavily T2-weighted sequences in 84.4% cases (in 84.4% in axial plane, 68.8% in sagittal and 84.4% parasagittal parallel to the VI-th cranial nerve in the pontine cistern). Dorello’s canal was identified in 27/32 abducent nerves (84.4%) on the submillimetric, heavily T2-weighted sequence in parasagittal parallel to the abducent nerve in the pontine cistern plane. In 71.9% (23/32) of cases, the abducent nerve was in contact with the arterial vessel in pontine cistern.
Using axonal retrograde tracing, combined with morphometric analysis, we compared the distribution and number of claustral neurons projecting to the motor and somatosensory cortical areas in the Wistar rat. Comparable volumes of the retrograde tracer Fluoro-Gold, were injected into the motor or somatosensory cortices. Injections into these areas resulted in labeling of neurons along the entire length of the claustrum. Neurons retrogradely labeled after injection into the motor cortex prevailed in the anterior part of the claustrum, whereas those projecting to the somatosensory cortex predominated in the central part. The mean number of claustral neurons retrogradely labeled after tracer injections into the motor cortex significantly outnumbered that from the somatosensory cortical area (p<0.01). Similarly, the mean value of the numerical density of the retrogradely labeled neurons was significantly higher for the motor projection zone in the claustrum, than for the somatosensory projection zone (p<0.001). The contralateral claustral projections, both into the motor and somatosensory cortices, were considerably lower in number than the ipsilateral ones. These findings indicate that: (1) the claustral projections to the various cortical regions seem to be differentiated (2) the distribution of claustral neurons projecting to the motor and somatosensory neocortical areas shows an anteroposterior gradient, (3) the claustrum of the rat appears to be more closely related to the motor than to the somatosensory system, (4) the rat claustrum seems to function more as a satellite than a relay structure in relationship to the cerebral cortex.
Expression of substance-P in human neurons of trigeminal ganglia has been investigated by immunohistochemistry and morphometry. These neurons constituted 12.8% to 32.6% of the total neuronal population in the trigeminal ganglia. Substance-P positive granulations were concentrated around the nucleus, distributed focally in neuroplasm or dispersed over the neuroplasm. Morphometric analysis has indicated the presence of three populations of SP-positive cells: small, medium-sized and large. The results suggest a functional differentiation on the level of the first neurons of the afferent path of the stomatognatic system. Substance-P is likely to play a role in the transmission not only of nociceptive impulses but also of those involved in the mechano-functional stimulation of system activities.
The quantitative analysis of the claustrocortical connections labeled with the fluorescent retrograde tracer Fluoro-Gold (FG) was conducted on 90 rabbits subdivided into the following age groups (P2, P7, P14, P21, P30, P60, P90, P120, P180). The equal volumes of retrograde fluorescent tracer FluoroGold (FG) were injected into the selected regions of the motor or somatosensory cortices. The volume of the dorsal part of the claustrum, total number of projecting neurons, numerical density and percentage distribution of projecting neurons were estimated by means of the unbiased stereological methods. The claustrocortical connections both with the motor and somatosensory areas in a rabbit are established in the postnatal life. The parts of the claustrum occupied by the motor and somatosensory projection zones as well as the morphology of the cortically projecting neurons do not reveal characteristic changes during the studied period. The significant decrease of the total number and numerical density of cortically projecting neurons as well as the increase of the claustral volume may reflect the process of adjustment of the claustrum to its modulatory function upon corresponding cortical areas. The intensity of the claustral connections with the motor and somatosensory cortices reveals significant difference during the studied period, being higher for the motor projection. It may be assumed that the claustrocortical connections established before birth undergo significant quantitative changes during postnatal development.
The shape of the inferior horn of the lateral ventricle has been investigated in 100 (50right and 50 left) human brain hemispheres which were fixed in 40% formaldehyde solution and cut frontally. It has been found that the shape of the inferior horn depends on the course and depth of the collateral and occipitotemporal sulci. In most cases a part from two main ventricular surfaces: The hippocampal and superolateral, there is one surface more, either the inferior - collateral eminence (Type I - 97% of cases in the posterior part of the inferior horn, 49%- in the middle and 42%- in the anterior part) or the inferolateral, adjacent to the occipitotemporal sulcus ( Type II- 0%, 26% and 35%, respectively). In a few cases both collateral eminence and inferolateral surfaces were present (Type III- 3% , 2% and 6% respectively). In type IV neither collateral eminence nor the inferolateral surface appeared (0%, 23%, 17% respectively). The authors suggest to designate the inferolateral surface, not mentioned in the literature, as the occipitotemporal eminence (of the inferior horn).
An immunocytochemical double-staining method was applied in order to study the co-localisation of nitric oxide synthase (NOS) with three calcium-binding proteins, calbindin D28k (CB), calretinin (CR) and parvalbumin (PV) in the claustrum of the rat during the first 4 months of life (postnatal days: P0–P120). The co-localisation of NOS/PV and NOS/CB is reported. These neurons fall into the category of non-pyramidal cells. Double-labelled NOS/CB neurons are observed in the claustrum starting from P4, whereas double-labelled NOS/PV neurons are observed from P14 onwards. The percentages of double-labelled neurons increase in relation to the age. Double-labelled NOS/CB and NOS/PV neurons, although they do not constitute a numerous population, play an important role in the process of maturation of the claustrum. This is confirmed by the occurrence of these types of neurons at definite stages of maturation and by the increase in their number.
The hippocampus plays a role in new learning, memory and emotion and is a component of the neuroanatomical stress circuit. The structure is involved in terminating hypothalamic-pituitary-adrenocortical (HPA) axis responses to stress and attenuates stress responses by shutting off this axis. The immunoreactivity (-ir) of c-Fos, NGF and its receptor TrkA following acute and chronic open-field stress were studied in CA1-CA3 and the DG of the hippocampus. The material consisted of 21 male adult rats divided into three groups: nonstressed (control) animals and rats exposed to acute (15 min once) and chronic (15 min daily for 21 days) aversive stimulation (open-field exposure). The brains were stained with use of immunohistochemical methods for c-Fos, NGF or TrkA. In the animals exposed to acute open-field stress the number of c-Fos-, TrkAand NGF-ir cells was higher in all the structures studied than in the control animals. However they were differentiated only in c-Fos immunoreactivity. In the rats exposed to chronic open-field stress the number of c-Fos-ir cells in the structures of the hippocampal formation studied was smaller than in rats exposed to acute stress and was comparable to that in the control group. No differences were observed between the groups exposed to acute and chronic stress in the number of TrkA-ir cells in the structures under investigation. The number of NGF-ir neurons in CA1 and CA2 was lower after exposure to chronic than after exposure to acute stress but was still higher than that in the control group. Our findings indicate that neurons of CA1-CA3 and the DG are engaged in the stress response after acute as well as chronic open-field exposure. This is probably related to the important role of the hippocampus in processing new spatial information as well as in the habituation processes, although these appear to have different mechanisms.
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