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Salinity and drought are important agro-environmental problems occurring separately as well as together with the combined occurrence increasing with time due to climate change. Screening of bread wheat genotypes against salinity or drought alone is common; however, little information is available on the response of wheat genotypes to a combination of these stresses. This study investigates the response of a salt-resistant (SARC-1) and a salt-sensitive (7-Cerros) wheat genotype to drought at different growth stages under non-saline (ECe 2.1 dS m-1) and saline soil (ECe 15 dS m-1) conditions. Drought was applied by withholding water for 21 days at a particular growth stage viz. tillering, booting, and grain filling stages. At booting stage measurements regarding water relations, leaf ionic composition and photosynthetic attributes were made. At maturity grain yield and different yield, components were recorded. Salinity and drought significantly decreased grain yield and different yield components with a higher decrease in the case of combined stress of salinity 9 drought. The complete drought treatment (drought at tillering + booting + grain filling stages) was most harmful for wheat followed by drought at booting stage and grain filling–tillering stages, respectively. The salt-resistant wheat genotype SARC-1 performed better than the saltsensitive genotype 7-Cerros in different stress treatments. A decrease in the water and turgor potentials, photosynthetic and transpiration rates, stomatal conductance, leaf K+, and increased leaf Na+ were the apparent causes of growth and yield reduction of bread wheat due to salinity, drought, and salinity 9 drought.
Sweet potato (Ipomoea batatas (L.) Lam) is an important crop due to its cultivation as staple food for millions of small farmers as well as for poor people in Latin America, Asia, Africa and in many other parts of the world. This tuberous crop is susceptible to drought stress especially during the period of crop establishment as well as vine development and tuber initiation. Yield of the crop vary widely among farmers due to improper planting systems. This current study was aimed to investigate the influence of various irrigation intervals and planting systems on vegetative growth, storage root yield and quality of sweet potato (Ipomoea batatas (L.) Lam) cv. ‘White star’ under field conditions. Three irrigation intervals (7, 14 and 21 days for summer crop, and 14, 28 and 42 days for winter crop) and two planting systems (bed planting and ridge planting) were adopted in this study. Vine length, number of branches and average leaf area significantly reduced as the irrigation interval was increased. Ridge planting produced longer vines with greater leaf area in winter crop as compared to bed planting. Yield parameters (storage root length, storage root diameter, number and fresh weight of marketable roots per plant) were directly linked with vegetative growth especially in summer crop. Under water stress conditions, as vegetative growth decreased storage root yield was also reduced. However, yield attributes were not affected by the planting systems. Vitamin C content decreased with water stress, whereas total soluble solids (TSS) and leaf proline content significantly increased with water stress in summer crop. Ridge planting also resulted in increased leaf proline content in summer crop. It is concluded that for attaining good vegetative growth and storage root yield, sweet potato should be irrigated at an interval of 7 days during summer and 14 days during winter crop and planted on ridges.
A field study was conducted to evaluate the effect of different plant growth regulators (PGRs) applied through seed priming and foliar spray on growth and yield of three okra cultivars grown under calcareous soils. The cultivars of Punjab Selection and Sabzpari produced significantly higher number of branches and leaves per plant, pod length and diameter and pod yield as compared to cv. Green ferry, while, cv. Punjab selection produced significantly greater number of flowers and pods per plant as compared to other two cultivars. Seed germination (%), plant height, and fruit set (%) were not affected by the cultivars. Among the PGR treatments, seed primed with GA3 resulted in significantly higher germination percentage and greater plant height at flowering. Seed priming and foliar spray with NAA and GA3 were effective in increasing the final plant height, number of branches, number of leaves, number of flowers and number of pods per plant and fruit set (%), fresh weight per pod and pod yield. However, pod diameter, pod moisture content and dry weight per pod were not influenced by the PGR treatments applied. These results suggested that the PGRs have great potential to improve seed germination, enhance growth and increase yield of okra cultivars under calcareous soils.
The objective of the present study was to determine the prevalence of brucellosis in household animals of Mirpur, Azad Kashmir due to its geographic importance. A total of 360 blood samples of cattle, buffaloes, sheep and goats were initially screened through Rose Bengal Plate test (RBPT) and then positive samples were subjected to Enzyme Linked Immunosorbent Assay (ELISA) for confirmation and quantification of antibody titers. Molecular confirmation of serologically positive samples was performed by real time polymerase chain reaction (PCR). RBPT and ELISA showed a total of 8.6% and 6.87% positive samples respectively. The species wise seropositivity by RBPT was greater in cattle followed by buffaloes, goats and sheep. Similarly ELISA showed more seropositivity in cattle than buffaloes, while sheep and goats were negative for brucellosis by ELISA. RT-PCR revealed 100% samples positive for Brucella abortus by species specific PCR. This study revealed the presence of Brucella abortus in Mirpur for the first time. Since brucellosis is listed in transboundary diseases, its presence in this geographically important region could be a potential threat for neighboring countries.
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