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1

Resilience of energy and CO2 exchange to a summer heatwave in an alpine humid grassland on the Qinghai-Tibetan Plateau

100%

Zhang F., Cao G.

Polish Journal of Environmental Studies

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2017

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tom 26

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nr 1

Summer heatwaves are expected to be much more frequent and severe with negative effects on terrestrial ecosystem water and carbon budgets, while the impacts on alpine grasslands remain poorly understood. Here we analyzed eddy flux and meteorological dataset of a seven-day (July 26 to August 1) summer heatwave in an alpine humid grassland in northeastern Qinghai-Tibetan Plateau in 2015. Compared with pre-heatwave, only diurnal ecosystem respiration (RES) increased by 30.7%, evidently (P < 0.001) during the heatwave. Diurnal sensible heat fluxes (H) and latent heat fluxes (LET) increased by 18.1% (P = 0.08) and 27.5% (P = 0.02) from 9:00 to 16:00. The heatwave did not lead to substantial increments of daily H and daily LET, while daily Bowen ratio (H/LET) decreased a little (P = 0.07). Daily net ecosystem CO₂ exchange increased by 76.7% (P = 0.03), mainly resulting from remarkable growth in daily RES (P<0.001) and undetectable fluctuation in daily gross primary production (GPP) (P = 0.13). Daily ecosystem water use efficiency (GPP/evapotranspiration) decreased by 20.8%. The little difference of energy and CO₂ fluxes between pre-heatwave and post-heatwave indicated strong resilience to the summer heatwave in the alpine humid grassland. Our results revealed that the present-day summer heatwave exerted a limited influence on energy exchange and vegetation photosynthetic activity but did stimulate ecosystem respiration, which would provide a positive feedback to climate warming with more carbon efflux from alpine grassland.

2

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Hypoxia-induced programmed cell death in root-tip meristematic cells of Triticum aestivum L.

100%

Pang N., Zhang F.

Acta Biologica Cracoviensia. Series Botanica

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2015

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tom 57

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nr 1

In this study, wheat (Triticum aestivum L.) roots were treated with hypoxic water. The staining of cell preparations with DAPI revealed morphological changes of the cells such as nuclear condensation, deformation and fragmentation. Under TEM, cellular membrane shrinkage and breakage, chromatin condensation and apoptotic- like bodies were displayed. The number of mitochondria increased dramatically; their cristae were damaged; the interior became a cavitation and only some flocculent materials were distributed. Indirect immunofluorescence staining indicated that cytochrome C diffused from mitochondria to nucleoplasm and cytoplasm. TUNEL positive nuclei indicated double strand breaks of DNA. DAB staining was used for the identification of hydrogen peroxide and examination showed that the longer the treating time, the darker the staining of the meristematic zones of the roots which suggested the increased accumulation of these Reactive Oxygen Species (ROS). The elevation of hydrogen peroxide production was paralleled with the increase of SOD and POD activities. A negative correlation between the exposure time under hypoxia and the contents of soluble proteins was found. No obvious effect of hypoxia on MDA was established. The obtained results demonstrate that hypoxia causes programmed cell death in the root-tip meristematic cells of Triticum aestivum L. which is most probably attributed to the accumulation of large amounts of ROS.

3

Wetlands appraisal method to alleviate urban heat island effect

81%

Zhang F., Shao D., Shao Y.

Polish Journal of Environmental Studies

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2014

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tom 23

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nr 5

Hangzhou is in the process of becoming a world-famous city known for its quality of life and advancement of environmental protection. This research evaluates how the city’s surrounding environment is affected by the development of urban wetlands. It is simulated upon the numerical model on an urban scale, with the full advantage of meteorological data and updated techniques related to RS (remote sensing), GIS (geographic information system), and meteorological numerical simulation. Results with figures and meteorological data show that urban wetlands in Hangzhou alleviate the urban heat island effect as well as high temperatures in summer, and significant economic benefits can also be derived from this environmental improvement.

4

Conversion of cropland to grassland and forest mitigates global warming potential in Northeast China

81%

Miao S., Qiao Y., Zhang F.

Polish Journal of Environmental Studies

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2015

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tom 24

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nr 3

In converting cropland to grassland and forest, more carbon is sequestered in grassland soil and forest biomass, but the mitigation of global warming potential (GWP) is not clear. In this study, we use the longterm conversion from cropland to grassland (28 y) and forest (14 y) to comprehensively assess the impact on GWP of soil carbon (C), nitrogen (N), CO₂, and N₂O emissions. The results showed that compared to the original cropland, conversion to grassland increased soil C content by 51.1%, soil N content by 28.4%, soil C stock (SCS) by four times, CO₂ emission by 17%, and N₂O emission by 40%; soil N stock (SNS) decreased by half. The corresponding values after afforestation were 7.2%, 5.2%, three times, 3%, -80%, and half, respectively. Overall GWP in the cropland system was calculated using the fuel used for farming production, the change in soil C, and N₂O emissions. Due to large C sequestration, the GWP of conversion to grassland (-1667 kg CO₂-C equivalent ha⁻¹·y⁻¹) and forest (-324 kg CO₂-C equivalent ha⁻¹·y⁻¹) were significantly lower than the cropland system (755 kg CO₂-C equivalent ha⁻¹·y⁻¹). The relationship between GWP and greenhouse gas, between GWP and the change of total C and N, suggest that in rain-fed agricultural systems in northeast China, the conversion from cropland to grassland and forest can mitigate GWP through changing CO₂ and N₂O emissions.

5

The suppressive effect of co-inhibiting PD-1 and CTLA-4 expression on H22 hepatomas in mice

81%

Liang L., Ge K., Zhang F., Ge Y.

Cellular and Molecular Biology Letters

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2018

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tom 23

Objective: We investigated the suppressive effect of siRNA-mediated co-inhibition of PD-1 and CTLA-4 expression on H22 hepatomas in mice. Methods: Murine H22 cells were cultured in vivo in ICR mice. An allograft tumor model was also established in another ICR mouse group. The tumor-bearing mice were randomly divided into four groups: control, single PD-1 siRNA, single CTLA-4 siRNA, and double PD-1 + CTLA-4 siRNAs. The survival time and physiological condition of the mice were observed after the injection of the siRNAs and placebo. The volume and weight of the solid tumor were measured to assess the inhibition of the tumor. To assess the effects of siRNAs on mouse immune function, the protein levels of IFN-γ and IL-10 in the blood and PD-L1 in the tumor and liver were determined using ELISA, and the mRNA levels of IFN-γ, PD-L1, PD-1, CTLA-4, IL-6 and Survivin in the tumor, liver and spleen were determined using quantitative RT-PCR. The ratios of Bax and Bcl-2 protein were determined via western blot to analyze the effect of siRNAs on tumor cell apoptosis. Results: The anti-tumor effect appeared in all groups with siRNA-mediated inhibition. The tumor growth suppression was stronger in the group with double inhibition. The weight and volume of the tumors were significantly lower and the survival rate improved in the three siRNA groups. IFN-γ levels increased but IL-10 levels decreased in the blood of the siRNA group mice compared with the results for the control group. In the tumor and spleen tissue, the IFN-γ levels significantly increased, but in the liver tissue they significantly decreased in the three siRNA groups. The results of quantitative RT-PCR showed that the mRNAs for PD-1 and CTLA-4 were downregulated in spleen tissue in the three siRNA groups, while the PD-L1 mRNA and protein levels increased significantly in the tumor, but decreased in the liver. Survivin and IL-6 mRNA levels decreased in the tumor. Western blot results showed that ratio of Bax and Bcl-2 had significantly increased. These results indicated that downregulating PD-1 and CTLA-4 could increase the body’s immune response and promote apoptosis of tumor cells. Conclusion: Co-inhibiting the expressions of PD-1 and CTLA-4 can effectively suppress the growth of H22 hepatoma and promote the apoptosis of tumor cells in mice. Blocking PD-1 and CTLA-4 can improve the vitality of T cells, and improve the immune environment and response.

6

LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots

81%

Lei X., Ma K., Zhang F.

Acta Biologica Cracoviensia. Series Botanica

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2021

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tom 63

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nr 1

7

Assessing 16 polycyclic aromatic hydrocarbons (PAHs) in river basin water and sediment regarding spatial-temporal distribution, partitioning, and ecological risks

81%

Chen Y., Sun C., Zhang J., Zhang F.

Polish Journal of Environmental Studies

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2018

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tom 27

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nr 2

This work investigated the distribution, partitioning, and ecological risks of 16 polycyclic aromatic hydrocarbons (PAHs) in water and sediment of the Yinma River Basin in China in normal, wet, and dry seasons. Sixteen PAHs were analyzed in 17 paired sets of water and sediment samples collected from the study area. The concentrations of PAHs in water and sediment showed spatial-temporal variations, the concentrations in water ranged from 167.6 to 373.9 ng/L with a mean value of 227.9 ng/L in the normal season, from 186.0 to 386.9 ng/L with a mean value of 281.6 ng/L in the wet season, and ranged from 147.0 to 315.9 ng/L with an average value of 218.6 ng/L in the dry season, while concentrations in sediment varied from 895.6 to 2,518.2 ng/g with a mean value of 1,739.4 ng/g in the normal season, from 1,233.0 to 4,763.0 ng/g with a mean value of 2,122.6 ng/g in the wet season, and ranged from 914.2 to 5,678.5 ng/g with an average value of 1,943.7 ng/g in the dry season. Partitioning of PAHs in water and sediment was studied and the result showed that PAHs tend to absorb in sediment. Ecological risk assessments were conducted based on the species-sensitivity distribution (SSD) model, with results suggesting that ecological risks of individual PAHs in water and sediment were little, and the multiple congeners of PAHs in sediment from some sampling sites had ecological risks. Primary sources of 16 PAHs in water and sediment were identified with isomer ratios, and results indicated that primary sources of PAHs in water were identified as a mixture pattern of petroleum and combustion sources, and in sediment were identified as a mixture pattern of petroleum and grass, wood and coal combustion sources.

8

QTL mapping of Fusarium moniliforme ear rot resistance in maize. 1. Map construction with microsatellite and AFLP markers

81%

Zhang F., Wan X. Q., Pan G. T.

Journal of Applied Genetics

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2006

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tom 47

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nr 1

To map the QTLs of Fusarium moniliforme ear rot resistance in Zea mays L., a total of 230 F₂ individuals, derived from a single cross between inbred maize lines R15 (resistant) and Ye478 (susceptible), were genotyped for genetic map construction using simple sequence repeat (SSR) markers and amplified fragment length polymorphism (AFLP) markers. We used 778 pairs of SSR primers and 63 combinations of AFLP primers to detect the polymorphisms between parents, R15 and Ye478. From the polymorphic 30 AFLP primer combinations and 159 SSR primers, we scored 260 loci in the F₂ population, among which 8 SSR and 13 AFLP loci could not be assigned to any of the linkage groups. An integrated molecular genetic linkage map was constructed by the remaining 151 SSR and 88 AFLP markers, which distributed throughout the 10 linkage groups of maize and spanned the genome of about 3463.5 cM with an average of 14.5 cM between two markers. On 4 chromosomes, we detected 5 putative segregation distortion regions (SDRs), including 2 new ones (SDR₂ and SDR₇). The other 3 SDRs were located near the regions where gametophyte genes were mapped, indicating that segregation distortion could be partially caused by gametophytic factors.

9

Wheat straw-derived biochar enhanced nitrification in a calcareous clay soil

71%

Wang C., Chen S., Wu L., Zhang F., Cui J.

Polish Journal of Environmental Studies

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2018

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tom 27

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nr 3

Biochar’s production and application in soils has been suggested as a means of abating climate change by sequestering carbon while simultaneously providing energy and increasing crop yields. However, little is known about biochar’s effect on nitrification in alkaline soil. This study focused on the effect of wheat straw-derived biochar (0%, 2%, 5%, and 10%, w/w) on nitrification in a calcareous clay soil with an incubation experiment. Moreover, the variations of ammonia-oxidizing bacteria (AOB) amount, urease activity, pH, and inorganic nitrogen contents during the incubation and their relationships with potential nitrification rates were also explored. The results indicated that nitrification was enhanced by wheat straw-derived biochar and showed an obvious dose-response to biochar application rate. Generally, the potential nitrification rate increased with incubation time elapsing for all four treatments, which were in the ranges of 21.0-33.9, 23.7-45.1, 21.4-57.5, and 31.8-66.1 nmol N/(g dry soil∙h), respectively. The potential nitrification rate increased by 1.36-2.40 times at 10% biochar application rate compared with the control (0%) at the same incubation stage. Except for NH₄⁺-N content of the soil-biochar mixture, AOB amount, urease activity, pH, and NO₃⁻-N content all showed increasing trends during incubation. Moreover, correlation analysis indicated that the potential nitrification rate was positively correlated with AOB amount, urease activity, pH, and NO₃⁻-N content (r≥0.713, P<0.01), but negatively correlated with NH₄⁺-N content (r = -0.408, P<0.01). Notably, though the biochar application in the Loess Plateau region has more benefits for soil condition improvement, the enhanced nitrification induced by biochar may pose a negative effect on fertilizer bioavailable efficiency in the agricultural system.

10

Morphology of the invasive amphiphyte Alternanthera philoxeroides under different water levels and nitrogen concentrations

71%

Ding W., Zhang H., Zhang F., Wang L., Cui S.

Acta Biologica Cracoviensia. Series Botanica

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2014

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tom 56

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nr 2

11

The effects of different types of biochar on ammonia emissions during co-composting poultry manure with a corn leaf

71%

Rong R., Zheng Y., Zhang F., Yang L., Li Z.

Polish Journal of Environmental Studies

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2019

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tom 28

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nr 5

Composting of poultry manure (PM) can cause several problems, including ammonia (NH₃) emissions. Biochar is a useful material in the composting process due to its microstructure and sorption properties. This research aims to investigate the changes in NH₃ emissions and its relationship with physicochemical properties and the bacterial community during PM composting with different biochar. Seven treatments were carried out with a 2:1 ratio of PM and corn leaf (dry weight basis), i.e. compost with no biochar (control); compost with 5%, 10% and 15% wheat stalk biochar (WSB); and compost with 5%, 10% and 15% rice husk biochar (RHB). The results indicated that adding WSB and RHB could obviously (p<0.05) reduce NH₃ emissions by 53.4-88.7% and 62.5-89.3%, respectively. And the volatilization of NH₃ in RHB treatment was lower than that in WSB treatment at the same ratio. Higher surface areas of RHB could adsorb more NH₄⁺ and NH₃ to reduce the volatilization of NH₃ at the thermophilic composting stage. The analysis of the bacterial community in compost indicated that adding biochar could decrease the population of Ureibacillus thermosphaericus involved in the oxidative deamination process, which further reduced the NH₄⁺-N content and the emission of NH₃. Thus, RHB is suggested to be added into PM composting with a ratio of 15%.

12

Heavy metals pollution characteristics and health risk assessment of farmland soils and agricultural products in a mining area of Henan Province, China

71%

Zhang F., He Y., Zhao C., Kou Y., Huang K.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 5

13

Comparison of salt tolerance between Cichorium intybus L. transformed with AtNHX1 or HvBADH1

71%

Zhang F., Li X., Lai P., Li P., Zhao Y.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 02

Drought and salinity are two major limiting factors of plant growth and agricultural productivity. An efficient way to improve salt tolerance of plants is by expression of the Na+/H+ antiporter gene AtNHX1 from Arabidopsis thaliana, introduced via genetic transformation. Similarly, expression of the coding genes of betaine aldehyde dehydrogenase (BADH) cloned from Graminaceous or Chenopodiaceous weeds appears to increase salt tolerance of plants. Unlike AtNHX1 gene, the transformation of BADH genes additionally enhances osmotic stress tolerance to the transformants. To better understand the differences of their capacities in promoting plant salt tolerance, the HvBADH1 gene from Hulless barley and the AtNHX1 gene, were introduced into a glycophyte species Cichorium intybus L. We investigated the traits of osmotic adjustment and antioxidation ability in the transformed plants under salt stress condition. The results indicated that both AtNHX1 and HvBADH1-transformed plants showed similar Na+ and K+ accumulations, but HvBADH1-transformed plants exhibited better osmotic adjustments to salt stress. And the AtNHX1 overexpression lines exhibited superior membrane protection and relative calli growth, delivering better NaCl tolerance to the plants under conditions of severe salt stress.

14

EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

71%

Zhang C., Shi W., Ma K., Li H., Zhang F.

Acta Societatis Botanicorum Poloniae

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2016

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tom 85

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nr 3

In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca²+) chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.

15

Experimental and mathematical investigations on unconfined compressive behaviour of costal soft soil under complicated freezing processes

71%

Wang W., Li N., Zhang F., Zhou A., Chi S.

Polish Maritime Research

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2016

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tom 23

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nr 4

In order to properly understand the effect of freezing-thawing circle (FTC) to mechanical behavior of costal soft soil (CSS), unconfined compressive test is conducted. Six kind FTC times are designed from zero to five. The tested data show that: (1) unconfined compressive strength of CCS decreases nonlinearly with more FTC, and the strength after five FTC times becomes about 22% of its original strength without any freezing-thawing experience; (2) stressstrain curves of all unconfined compressive samples can be well fitted by three-parameter hyperbolic model; (3) and relationship between two parameters and FTC times can be fitted by exponent function, while another parameter can be considered as 0.95. Consequently, one composite hyperbolic- exponent empirical formula is established in order to describe freezing-thawing-dependent stress-strain behavior of CSS. Finally, good agreements have been found between tested dada and simulated results

16

The novel protein C3orf43 accelerates hepatocyte proliferation

71%

Zhang C., Chang F., Li D., Zhang F., Xu C.

Cellular and Molecular Biology Letters

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2017

|

tom 22

17

Interactive effects of BDE-153 and its metabolite 2,4-DBP on biochemical status in crucian carp (Carassius auratus)

71%

Lu G., Liu J., Zhang F., Nkoom M., Wu D.

Polish Journal of Environmental Studies

|

2018

|

tom 27

|

nr 3

Polybrominated diphenyl ethers (PBDEs), which belong to the class of brominated flame-retardants, are widely used in industrial products. PBDEs have been detected in varied environmental matrices and they can induce various toxicities such as neurotoxicity, cytotoxicity, and endocrine-disrupting effects in animals. The main objective of this study was to investigate the effects of 2,2’,4,4’,5,5’-hexabromodiphenyl ether (BDE-153), 2,4-dibromophenol (2,4-DBP), and their mixtures on the endocrine system, including acetylcholinesterase (AChE) of brain, ethoxyresorufin-O-deethylase (EROD), glutathione-S-transferase (GST), and superoxide dismutase (SOD) of liver in crucian carp (Carassius auratus). Fish were exposed to BDE-153 (0.2-100 mg/kg in food), 2,4-DBP (0.2-100 mg/kg in food), and their mixture for seven days. BDE-153 alone or in combination with 2,4-DBP significantly reduced brain AChE activity and increased liver EROD activity while no significant effects were observed for 2,4-DBP alone. The simultaneously elevated GST and SOD activities were found at higher doses of BDE-153 and 2,4-DBP (alone and in combination), and exhibited obvious positive correlation (0.76

18

Water- and N-induced changes in soil C:N:P stoichiometry and its implications for N limitation of a desert steppe species, Glycyrrhiza uralensis

71%

Huang J., Yu H., Zhang F., Li M., Lin H.

Polish Journal of Ecology

|

2016

|

tom 64

|

nr 2

Changes in precipitation patterns and the deposition of atmospheric nitrogen (N) increase the possibility of altering soil carbon (C):N:phosphorus (P) stoichiometry through their effects on soil C and nutrient dynamics, especially in water- and N-limited ecosystems. We conducted separate 2-year watering and N addition experiments, and examined soil C:N:P stoichiometry, relative growth rate, and leaf N resorption traits of Glycyrrhiza uralensis Fisch in a desert steppe of northwestern China. Our objectives were to determine how soil C:N:P stoichiometry responded to climate change, and its indications for plant growth and N resorption. The results showed that additional water increased N loss and thus decreased N availability, resulting in high N resorption from senescing leaves of G. uralensis. N addition increased N availability, consequently reducing plant N dependence on leaf resorption. High relative growth rates occurred with intermediate N:P and C:N ratios, while high N resorption occurred with a low N:P ratio but a high C:N ratio. Our results indicate that soil C:N:P stoichiometry also could be a good indicator of N limitation for desert steppe species. Altered soil C:N:P stoichiometry affects the N strategy of plants, and will be expected to further influence the structure and function of the desert steppe community in the near future.

19

Evidence of TWIST1 and transforming growth factor-beta1 aberrant expressions as novel therapeutic targets in modulating the severity of osteoarthritis with focus on biologic agents

71%

Ye C., Ye J., Wu H., Zhang F., Liu Y.

Journal of Physiology and Pharmacology

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2020

|

tom 71

|

nr 6

20

Isolation of the cDNAs encoding secreted and membrane binding proteins from egg of Schistosoma japonicum [Chinese strain]

71%

Yu C., Zhang F., Yin X., Kikuchi M., Hirayama K.

Acta Parasitologica

|

2008

|

tom 53

|

nr 1

The granulomatous reaction which occurs around egg trapped in the intrahepatic venules ultimately may lead to fibrosis, which is the main pathogenesis of schistosomiasis. The excreted proteins from eggs play an important role during this process, and they may be a target for developing new strategies to control the hepatic pathogenesis caused by schistosome infection. In this study, fifteen genes encoding secreted or membrane binding protein were identified with the signal sequence trapping method by retrovirus mediated expression screening (SST-REX) of cDNAs from the egg of Schistosoma japonicum (Chinese strain).

Ograniczanie wyników

Resilience of energy and CO2 exchange to a summer heatwave in an alpine humid grassland on the Qinghai-Tibetan Plateau

Hypoxia-induced programmed cell death in root-tip meristematic cells of Triticum aestivum L.

Wetlands appraisal method to alleviate urban heat island effect

Conversion of cropland to grassland and forest mitigates global warming potential in Northeast China

The suppressive effect of co-inhibiting PD-1 and CTLA-4 expression on H22 hepatomas in mice

LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots

Assessing 16 polycyclic aromatic hydrocarbons (PAHs) in river basin water and sediment regarding spatial-temporal distribution, partitioning, and ecological risks

QTL mapping of Fusarium moniliforme ear rot resistance in maize. 1. Map construction with microsatellite and AFLP markers

Wheat straw-derived biochar enhanced nitrification in a calcareous clay soil

Morphology of the invasive amphiphyte Alternanthera philoxeroides under different water levels and nitrogen concentrations

The effects of different types of biochar on ammonia emissions during co-composting poultry manure with a corn leaf

Heavy metals pollution characteristics and health risk assessment of farmland soils and agricultural products in a mining area of Henan Province, China

Comparison of salt tolerance between Cichorium intybus L. transformed with AtNHX1 or HvBADH1

EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

Experimental and mathematical investigations on unconfined compressive behaviour of costal soft soil under complicated freezing processes

The novel protein C3orf43 accelerates hepatocyte proliferation

Interactive effects of BDE-153 and its metabolite 2,4-DBP on biochemical status in crucian carp (Carassius auratus)

Water- and N-induced changes in soil C:N:P stoichiometry and its implications for N limitation of a desert steppe species, Glycyrrhiza uralensis

Evidence of TWIST1 and transforming growth factor-beta1 aberrant expressions as novel therapeutic targets in modulating the severity of osteoarthritis with focus on biologic agents

Isolation of the cDNAs encoding secreted and membrane binding proteins from egg of Schistosoma japonicum [Chinese strain]