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Nasiona cebuli odmiany 'Wolska' poddano hydrokondycjonowaniu kilkoma sposobami: (1) umieszczono je na 6 lub 7 godz. w kolumnie wypełnionej napowietrzaną wodą destylowaną o temperaturze 20°C, (2) przetrzymywano je przez 2 dni w temperaturze 15°C lub 20°C po dodaniu 400 lub 500 μ1 wody destylowanej na 1 g nasion, (3) moczono je przez 3 godz. w wodzie o temperaturze 20°C, a następnie umieszczano na 3 dni w temperaturze 20°C oraz wilgotności względnej powietrza około 100%. Nasiona poddano również osmokondycjonowaniu w roztworze glikolu polietylenowego (PEG 8000) o potencjale osmotycznym -1,5 MPa przez 7 dni w ciemności w temperaturze 15°C. Po hydro- i osmokondycjonowaniu nasiona suszono, a następnie oceniano ich kiełkowanie w temperaturze 10°C i 20°C. Osmokondycjonowanie, a następnie hydrokondycjonowanie w 500 μl wody na 1 g nasion w temperaturze 20°C, najbardziej przyspieszyło kiełkowanie nasion w temperaturze 10°C. Kondycjonowanie nasion w atmosferze nasyconej parą wodną skróciło natomiast w największym stopniu ich czas kiełkowania w temperaturze 20°C. Przetrzymywanie nasion w temperaturze 15°C lub 20°C po dodaniu 500 μl wody na 1 g nasion i w 20°C po dodaniu 400 μ1 wody na 1 g nasion oraz ich kondycjonowanie w atmosferze nasyconej parą wodną poprawiło zdolność kiełkowania nasion w temperaturze 10°C.
Introduction: Milk thistle (Silybum marianum (L.) Gaertn.) is a medicinal plant belonging to Asteraceae family. Extract from milk thistle achenes (termed in practice as seeds) contains sylimarin, which protects liver cells against inorganic and organic toxic compounds. Objective: The aim of the research was to evaluate the effect of colour grading on the quality of milk thistle seeds. Methods: Seeds were graded manually by colour according to the Royal Horticultural Society Colour Chart, issued in Great Britain. In three samples two fractions of seeds were separated: beige and brown, whereas seeds of the fourth sample were graded into three fractions: yellow, beige and brown. The 1000-seed weight and germination of graded and non-graded seeds were evaluated. Infestation of graded seeds with fungi was assessed. Results: Brown seeds had a higher 1000-seed weight than beige or yellow ones. Germination at the final count of beige seeds did not differ as compared to that of brown seeds or was even better. Milk thistle seeds were infested with numerous fungi, however Alternaria alternata and Ulocladium consortiale predominated. Conclusions: Less mature beige seeds can be used as sowing material because their germination at the final count did not differ as compared to that of fully mature brown seeds or was even better. Infestation of these seeds with some of the fungi was lower than brown seeds.
Seed infection with Alternaria spp. is an important source of severe carrot diseases. The aim of the study was to evaluate the effects of carrot seed treatments with acetic acid, grapefruit extract and volatile compounds of fir (Abies alba Mill.) and thyme (Thymus vulgaris L.) essential oils on their germination, vigour and health. Seeds of two samples were soaked for 30 min in 0.5, 1 and 2% acetic acid, 0.5 and 1% Biosept Active (33% of grapefruit extract), or treated for 72 h or 96 h with volatile compounds of fir and thyme oils individually (10 µl), and jointly (5+5 µl). Controls were untreated seeds and seeds soaked in distilled water. Acetic acid effectively controlled A. alternata and A. radicina in both samples, and did not affect adversely seed germination, however, at the highest concentration deteriorated seed vigour. Biosept Active was less effective, however at 1% concentration decreased seeds infestation with A. alternata in sample I, and at concentrations of 0.5 and 1% reduced percentages of seeds infested with A. alternata and A. radicina in sample II. Essential oils treatments in some cases favoured growth of A. dauci and A. radicina.
Priming is a treatment applied for seed enhancement. Brassica vegetable seeds are commonly affected by Alternaria brassicicola. The changes in germination and vigour of kohlrabi (Brassica oleracea L. var. gongylodes L.) seeds subjected to two priming methods in the presence of A. brassicicola were investigated. The following treatments were applied: uninoculated non-primed seeds (control), seeds inoculated with A. brassicicola (2∙104 spores ml-1), uninoculated and inoculated seeds hydroprimed for 1–2 days, and uninoculated and inoculated seeds osmoprimed for 1–4 days. The percentage of germinating seeds, germination capacity, the percentages of diseased seedlings and dead seeds were determined. The speed of germination, tetrazolium staining index, and electrical conductivity described seed vigour. The presence of A. brassicicola on/in seeds was confirmed on CW semiselective agar. Osmopriming was conducive to penetration of A. brassicicola into kohlrabi seeds. The presence of the fungus had no influence on the percentage of germinating seeds, but a significantly lower germination capacity and a higher percentage of diseased seedlings and dead seeds were observed after inoculation. Non-primed inoculated seeds germinated faster than uninoculated ones. Despite the presence of A. brassicicola, osmotic priming improved seed vigour as measured by the speed of seed germination and electrical conductivity. The results of the tetrazolium vigour test showed its limited usefulness for seeds heavily infected with A. brassicicola.
Three seed priming techniques: hydropriming, halopriming and osmopriming, were compared for their effects on germination and vigour of pansy (Viola × wittrockiana Gams.) seeds at 20°C, 30°C and 35°C. Seeds were hydroprimed in the restricted volumes of water (600 and 700 μl H2O·g seed-1, 2, 3 or 4 days), haloprimed in KNO3 solution (-1.5 MPa, 5 days), and osmoprimed in polyethylene glycol solutions (-1.0, -1.25 or -1.5 MPa PEG 8000, 7 days) at 15°C or 20°C. Seed germination and vigour tests were performed for untreated and primed seeds. Generally, hydropriming negatively affected the speed of germination, the percentage of germinating seeds and germination capacity. Halopriming accelerated seed germination at 20°C, 30°C and 35°C but did not influence the percentage of germinating seeds and germination capacity. Osmopriming of seeds in PEG solution of osmotic potential –1.0 MPa at 20°C not only improved germination rates at 20°C, 30°C, and 35°C to the highest extent, but also increased percentage of germinating seeds at 30°C and 35°C most effectively and positively affected seed germination capacity at 20°C and 30°C.
Priming is one of the most common methods improving seed quality. The purpose of the research was to study effects of osmopriming on milk thistle seed germination, vigour, health and location of fungi at 10 and 20°C. Seeds (achenes) of two samples (I, II) were primed in the polyethylene glycol (PEG) solutions of osmotic potentials of -1.0, -1.25 and -1.5 MPa, at 15°C for 3, 5 and 7 days. Applied variants of priming improved energy of seed germination and germination capacity at 10°C in both samples. Enhancement of these parameters was also observed at 20°C, if seeds of sample I were primed in the PEG solutions of osmotic potentials of -1.0 MPa for 7 days and -1.25 and -1.5 MPa for 5 and 7 days. Priming, regardless of time and osmotic potential of PEG, accelerated seed germination at 10 and 20°C in both samples. Fungi of genera: Alternaria, Cladosporium, Fusarium and Rhizopus were frequently identified on the seeds. After priming significantly increased seed infestation with fungi, especially in sample I. The fungi were more often detected in pericarps and seed coats than embryos. Many a time, after priming an increase in inner achene infestation was observed.
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