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Effects of tillage alteration on soil water content, maize crop water potential and grain yield under subtropical humid climate conditions

100%

Chen J., He Y., Li P.

International Agrophysics

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2021

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tom 35

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nr 1

2

Comparative proteomics analysis of young spikes of wheat in response to Fusarium graminearum infection

100%

Ding L., Li M., Li P., Cao J.

Acta Physiologiae Plantarum

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2017

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tom 39

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nr 12

3

Perspectives on novel vaccine development

100%

Wang Y. B., Wang L. P., Li P.

Polish Journal of Veterinary Sciences

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2018

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tom 21

|

nr 3

4

Relationship of changes in the fatty acid compositions and fruit softening in peach (Prunus persica L. Batsch)

100%

Duan Y., Dong X., Liu B., Li P.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 03

Fatty acid compositions of peach (Prunus persica L. Batsch) mesocarp tissues from ‘Kawanakajima Hakuto’ and its firm-fleshed mutant ‘Shuangjiuhong’ were examined by gas chromatography during the developmental stages from 20 days before to 20 days after fruit ripening. Fruits were harvested at 4-day intervals from July to September. The predominant fatty acids were linoleic, palmitic and linolenic acids with 27.66–48.93 %, 23.59–31.65 %, and 12.08–28.35 % in ‘Shuangjiuhong’, and 32.64–42.79 %, 23.53–28.95 %, 16.14–39.15 % in ‘Kawanakajima Hakuto’, respectively. Saturated fatty acids (palmitic and stearic acids) remained relatively constant throughout the ripeness period. On the contrast, from 15 days before ripening, notable decline in oleic acid and increase of linoleic and linolenic acids were observed in both cultivars. In addition, from 10 days before ripening, much lower levels of oleic and linolenic acids and higher proportion of linoleic acid were observed in ‘Shuangjiuhong’ than those found in ‘Kawanakajima Hakuto’. And notably higher SFA level, lower levels of UFA and IUFA in the firm-fleshed peach were investigated during those stages. Correlation analysis showed that oleic acid and SFA had very significantly positive, whereas linolenic acid, UFA and IUFA had significantly negative correlation with fruit firmness. These results above suggest that lower levels of oleic and linolenic acids, UFA and IUFA, and higher linoleic acid and SFA content represent fruits with firmer flesh and help to retain the fruit texture.

5

Effects of Bothriochloa ischaemum characteristics induced by nitrogen addition on the process of slope runoff and sediment yield

100%

Li P., Li B., Wang J., Liu G.

Polish Journal of Environmental Studies

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2021

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tom 30

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nr 1

6

Effectiveness of photochemical reflectance index to trace vertical and seasonal chlorophyll a/b ratio in Haloxylon ammodendron

100%

Nyongesah M. J., Wang Q., Li P.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 02

Vegetation indices such as photochemical reflectance index (PRI) have been applied in tracing various biophysical and biochemical parameters within plants. However, less attention has been focused on chlorophyll (Chl) a/b ratio in arid environment. Quantitative estimation of Chl a/b ratio through remote sensing may provide important information regarding carbon balance and stress response in desert plants. We investigated the potential of PRI to trace Chl a/b ratio in Haloxylon ammodendron trees at Gurbantu¨nggu¨t Desert, China. The results revealed a seasonal and vertical variation in correlations. PRI was negatively correlated with Chl a/b ratio in all datasets, with pronounced sensitivity to changes in light across the canopy where low coefficient of determination (R2 = 0.30) was obtained in bottom compared to the middle and top canopy assimilating branches. Seasonally, PRI–Chl a/b ratio relationship exhibited a higher R2 for May–June dataset (R2 = 0.62, P\0.001) and a lower RMSE of 0.18. A larger variability in both relationships was apparent when data from different heights were pooled together (total dataset). Although good correlation between seasonal PRI–Chl a/b relationship was found in top canopy branches, this relationship did not hold throughout the entire growing season. The identified normalized differential (ND) index (ND(510,570)) performed slightly better than the original PRI with R2 improvement of 1 and 4 % for May–June and July–September datasets, respectively, and more than 5 % improvement in R2 for within canopy datasets. In addition, both PRI and ND(510,570) performed poorly for pooled data. Therefore, within canopy variation and age-related changes must be considered when retrieving Chl a/b ratio through hyper-spectral remote sensing. Furthermore, both PRI and ND(510,570) must be applied with caution when dealing with desert species because of confounding factors related to strong erratic changes such as drought. Further studies on PRI mechanisms in different species under different environment are recommended.

7

First record of the genus Duriocoris Miller, 1940 [Heteroptera: Reduviidae: Stenopodainae] from China, with the description of a new species

100%

Han Y., Li P., Cai W.

Annales Zoologici

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2005

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tom 55

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nr 1

The species of the genus Duriocoris Miller, 1940 from China are reviewed. Two species are recognized, described or redescribed, illustrated. Duriocoris geniculatu is described as a new species. This genus and Duriocoris serratus Miller, 1940 are reported for the first time to China. A key to the three species of the genus is provided.

8

Redescription of the little known reduviid, Tiarodes elegans Stal, 1863 [Heteroptera: Reduviidae: Reduviinae]

100%

Li P., Liu H., Cai W.

Annales Zoologici

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2005

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tom 55

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nr 1

The little known reduviine species, Tiarodes elegatis Stål, 1863, is redescribed based on the material from Sri Lanka. The characters of male genitalia are reported for the first time. The dorsal habitus, head, male genitalia and other diagnostic morphological features are illustrated.

9

Aerodynamic performance of a new double-flap wing sail

88%

Li D., Zhang Y., Li P., Dai J., Li G.

Polish Maritime Research

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2019

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tom 26

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nr 4

As a type of natural energy resource, wind power is used in the modern implementation of wind-assisted technologies as a method for reducing the fuel consumption and environmental pollution of ocean-going ships. In order to promote the full usage of ocean wind energy for cargo ships, an innovative type of ship propulsion-assisted wing sail is proposed in this paper. The propulsion efficiency of this new wing sail can be increased by enlarging its area in both the transverse and vertical directions in good weather conditions, and it can be folded up automatically in poor weather conditions, improving the sailing safety of the ship. The sail parameters relating to the gaps and rotation angles between different parts of the wing sail are compared, and the values giving the best aerodynamic performance are identified using CFD simulation technology. The results for the lift and drag coefficients for the new wing sail at different attack angles are also compared with those of traditional aerofoil sails, including an arc-shaped rigid sail and a variable-camber sail proposed in 2015. From the viewpoint of the sailing performance of the vessel, our results demonstrate that this new type of wing sail has good aerodynamic performance and can reduce fuel costs for commercial vessels

10

Effects of solar ultraviolet radiation on photochemical efficiency of Chaetoceros curvisetus (Bacillariophyceae)

88%

Guan W. C., Li P., Jian J. B., Wang J. Y., Lu S. H.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 3

To assess the short- and long-term impacts of Ultraviolet radiation (UVR, 280–400 nm) on the red tide alga Chaetoceros curvisetus, we exposed cells to three different solar radiation treatments–PAB:280–700 nm, PA:320–700 nm, and P:400–700 nm under 20°C incubated temperature. Short-term exposures were investigated: the photochemical efficiency (ΦPSII) versus irradiance curves under six levels of solar radiation by covering the incubators with a variable number of neutral density screens (the irradiance thus varied from 100 to 3%) lasting 1 h, and longterm exposures were designed to assess how the cells acclimate to solar radiation (the growth, UVabc and ratio of repair to damage rates of D1 protein were detected). A significant decrease in the photochemical efficiency (ΦPSII) at high irradiance (100% of incident solar radiation, 261.6 Wm⁻²) was observed in short-term exposure (1 h). UVR-induced photoinhibition was reduced to 7% in 3% solar radiation (4.08 Wm⁻²), compared with 66% in 100% solar radiation (261.6 Wm⁻²). In long-term experiments (11 days) using batch cultures, cell densities during the first 6 days were relatively constant for treatment P, and decreased slightly under PA and PAB treaments, reflecting a change in the irradiance experienced in the laboratory to that of incident solar irradiance. Thereafter, cell density increased and UV-induced photoinhibition decreased with the following days, indicating acclimation to solar UV. At the end of experiment, cells were found to exhibit both higher ratios of repair to UV-related damage and increased concentrations of UV-absorbing compounds, whose maximum absorption was found to be at 329 nm. Our data indicate that C. curvisetus is sensitive to ultraviolet radiation, but was able to acclimate relatively rapidly (ca. 6 days) by synthesizing UV-absorbing compounds and by increasing the rates of repair processes of D1 protein in PSII.

11

Temporal and spatial variations in soil TOC, TN, and TP, and their relationships in bioretention tanks

88%

Guo C., Li J., Li H., Liu Z., Li P.

Polish Journal of Environmental Studies

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2018

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tom 27

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nr 6

This study aims to explore the temporal and spatial variations of soil total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), and the relationships between C/N, C/P and TOC, TN, and TP in bioretention tanks. Two bioretention tanks (tank No. 1: depth of 0~20 cm was vacant aquifer layer, 20~90cm, filled with planting soil, 90~105 cm, filled with gravel; tank No. 3: 0~20 cm was aquifer layer, 20~50 cm, filled with planting soil, 50~90 cm, filled with blast furnace slag and sand; 90~105cm, filled with gravel) were used in simulation stormwater runoff purification experiments to collect planting soil samples at intervals of one hour before inflow and 24 hours after the end of inflow. The results revealed that soil TN, TP, and TOC in 2 bioretention tanks were mainly concentrated at 10~30 cm in soil. The contents of TN and TP varied from 0.32 g kg⁻¹ to 0.50 g kg⁻¹ and from 0.83 g kg⁻¹ to 1.35 g kg⁻¹ within the investigated zone, respectively. Soil TN content in the 2 bioretention tanks before the inflow was slightly greater than after the inflow, but the opposite was true for TP, as it was less before the inflow than after. The potential of TN and TP fixation in No. 1 was higher than that in No, 3 within the upper 30 cm depth, which were related to the infiltration rate of underlying fillers (the underlying fillers of No. 1 is planting soil, and which is the blast furnace slag and sand in No. 3). The TOC content in the 2 bioretention tanks varied from 4.24 g kg⁻¹ to 8.97 g kg⁻¹, and the average contents decreased with the increasing depths. The C/N and C/P were positively correlated with TOC, while they were negatively correlated with TN and TP contents, which showed that soil C/N and C/P were mainly controlled by TOC in soil. The conclusions can provide references for the design and operation evaluation of bioretention facilities.

12

Comparison of salt tolerance between Cichorium intybus L. transformed with AtNHX1 or HvBADH1

88%

Zhang F., Li X., Lai P., Li P., Zhao Y.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 02

Drought and salinity are two major limiting factors of plant growth and agricultural productivity. An efficient way to improve salt tolerance of plants is by expression of the Na+/H+ antiporter gene AtNHX1 from Arabidopsis thaliana, introduced via genetic transformation. Similarly, expression of the coding genes of betaine aldehyde dehydrogenase (BADH) cloned from Graminaceous or Chenopodiaceous weeds appears to increase salt tolerance of plants. Unlike AtNHX1 gene, the transformation of BADH genes additionally enhances osmotic stress tolerance to the transformants. To better understand the differences of their capacities in promoting plant salt tolerance, the HvBADH1 gene from Hulless barley and the AtNHX1 gene, were introduced into a glycophyte species Cichorium intybus L. We investigated the traits of osmotic adjustment and antioxidation ability in the transformed plants under salt stress condition. The results indicated that both AtNHX1 and HvBADH1-transformed plants showed similar Na+ and K+ accumulations, but HvBADH1-transformed plants exhibited better osmotic adjustments to salt stress. And the AtNHX1 overexpression lines exhibited superior membrane protection and relative calli growth, delivering better NaCl tolerance to the plants under conditions of severe salt stress.

13

Analysis of driving forces of energy consumption in rural areas of China’s Henan Province based on the STIRPAT model and ridge regression

88%

Tian C., Li P., Shao H., Li M., Zheng Y.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 2

14

Transformation of wheat Triticum aestivum with the HvBADH1 transgene from hulless barley improves salinity-stress tolerance

76%

Li P., Cai J., Luo X., Chang T., Li J., Zhao Y., Xu Y.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 09

Studies have shown that the stress tolerance of cereal plants to osmotic or salinity stresses can be improved to varying degrees by the overexpression of an introduced betaine aldehyde dehydrogenase (BADH) gene. In the present study, the HvBADH1 gene from Hordeum vulgare L. var. nudum Hook. f., encoding a cytosolic BADH, was transferred into Triticum aestivum via traditional Agrobacterium tumefaciens-mediated transformation. Molecular methods, such as PCR, Southern blot analysis, and real-time quantitative RT-PCR were used to identify the successful integration and expression of the HvBADH1 transgene in genetically transformed wheat lines. To detect the efficacy of the HvBADH1 transgene in the transformants, some pivotal physiological indicators that reflected abiotic stress tolerance were measured in individual transgenic plant lines. These indicators included intracellular K⁺ and Na⁺ contents or K⁺/Na⁺ ratio, relative conductivity, and malondialdehyde and glycine betaine (GB) concentrations in cells. The results revealed that all the tested transgenic lines could significantly increase the recruitments of K⁺ in their cytosol than the wild-type seedlings. Similarly, 11.59- to 21.82-fold greater accumulation of GB, 2.11–2.56 times higher calli relative growth rates, and 26.2–29.1% seedling survival rates were found in transgenic lines under 150 mM NaCl stressed conditions. Our results demonstrated that by overexpressing the HvBADH1 transgene in genetically transformed wheat, the overall salt tolerance of the target plants was significantly increased, and the damaging effects of high salinity were significantly reduced.

15

Chemical reduction of nitrate using nanoscale bimetallic iron/copper particles

76%

Guo J., Yu M., Sun Z., Li P., Yang T., Liu J., Zhang L.

Polish Journal of Environmental Studies

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2018

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tom 27

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nr 5

Nanoscale zero-valent iron (NZVI) as an effective material has been applied to reduce nitrate. Yet NZVI has defects of aggregation and oxidation. To overcome these disadvantages, nanoscale bimetallic iron/copper particles were introduced to reduce nitrate in this work. In this paper, nanoscale bimetallic Fe/Cu particles were prepared by the liquid phase chemical reduction method; the particles were characterized by scanning electron microscopy (SEM) and x-ray diffraction (XRD). The effect of prepared particles was evaluated by reducing synthetic nitrate wastewater, and batch experiments were conducted to investigate the effect of initial nitrate concentration and various Cu loading on nitrate reduction by nanoscale bimetallic Fe/Cu particles. The results indicated that nitrate could be completely removed in 20 min reaction by nanoscale bimetallic Fe/Cu particles when Cu loading was 5% and initial nitrate concentration was 80 mg/L. As a result, the nitrate in wastewater was converted into ammonium and nitrogen gas, with nitrite as an intermediate by-product.

16

Systematic assessment of reference genes for RT-qPCR across plant species under salt stress and drought stress

76%

Xiao X., Wu X., Ma J., Li P., Li T., Yao Y.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 09

Suitable reference gene (RGs) is the prerequisite for accurate normalization of real-time quantitative PCR (RT-qPCR) data. However, previous results are diverse in various researches that focused on selecting stable RGs. This study aims at systematically assessing various RGs in plants under salt stress or drought stress by collection of geNorm rankings of genes, data transformation and statistic analysis. Although none of the analyzed genes can guarantee universally stable expressions in plant species under salt stress or drought stress, we found that 18S (18S ribosomal RNA) was generally the least stable gene under salt and drought stress. This gene should not be used as the RG in RT-qPCR. On the contrary, it is least risk to use EF1 for salt stress and TIP41 for drought treatment experiments. We compared the effects of salt and drought stresses on 7 frequently used RGs through paired-samples T test. The expression of Ubiquitin gene under drought stress is much more unstable than that under salt stress. The tested genes belonging to multigene family and having different stability could be one reason of variations in the published studies, which was supported by the analysis of expression profile of Salicornia europaea transcriptome. This is the first systematic assessment quantifying global stability of Rgs across plant species under salt stress and drought stress, which will improve our understanding of RGs and facilitate the future work on RGs selection.

17

Drought stress induces oxidative stress and the antioxidant defense system in ascorbate-deficient vtc1 mutants of Arabidopsis thaliana

76%

Niu Y., Wang Y., Li P., Zhang F., Liu H., Zheng G.

Acta Physiologiae Plantarum

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2013

|

tom 35

|

nr 04

Drought stress has a negative impact on plant cells and results in the generation of reactive oxygen species (ROS). To increase our understanding of the effects of drought stress on antioxidant processes, we investigated the response of the ascorbate-deficient Arabidopsis thaliana vtc1 mutant to drought stress. After drought stress, vtc1 mutants exhibited increases in several oxidative parameters, including H2O2 content and the production of thiobarbituric acid reactive substances. Decreases in chlorophyll content and chlorophyll fluorescence parameters were also observed. The vtc1 mutants had higher total glutathione than did wild-type (WT) plants after 48 h of drought stress. A reduced ratio of glutathione/total glutathione and an increased ratio of dehydroascorbate/total ascorbate were observed in the vtc1 mutants compared with the WT plants. In addition, the activities of enzymes that are responsible for ROS scavenging, including superoxide dismutase, catalase, and ascorbate peroxidase, were decreased in the vtc1 mutants compared with the WT plants. Similar reductions in activity in the vtc1 mutant were observed for the enzymes that are responsible for the regeneration of ascorbate and glutathione, including monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase. These results suggest that low intrinsic ascorbate and impaired ascorbate–glutathione cycling in the vtc1 mutant induced a decrease in the reduced form of ascorbate, which enhanced sensitivity to drought stress.

18

Sitefidelity and association patterns of Indo-Pacific humpback dolphins off the east coast of Zhanjing, China

76%

Xu X., Zhang Z., Ma L., Li P., Yang G., Zhou K.

Acta Theriologica

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2012

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tom 57

|

nr 2

This study examined the population of Indo-Pacific humpback dolphins, Sousa chinensis, inhabiting the waters off the east coast of Zhanjiang, China. A total of 116 dolphins were identified during 147 boat-based surveys, completed between June 2005 and June 2007. Abundance estimates indicated that a small population of 268 dolphins (95% CI = 189–413) inhabited this coastal area. The sighting frequencies of identified dolphins varied between one and five when the 2006 and 2007 data were organized into six occasions. Twenty-three percent (27 individuals) of the cataloged dolphins were identified in more than one calendar year. The two animals (ZJ001, ZJ011) with the highest degree of site fidelity were present in the study area for a period of 23 months. The most frequently (15 times) sighted dolphin (ZJ046) was recorded in nine (56%) of the 16 months surveyed. This evidence indicates the interannual site fidelity of S. chinensis to the area off the eastern coast of Zhanjiang City. Range sizes of eight identified individuals calculated by the minimum convex polygon varied from 2.07 to 331.20 km2. Associations between 34 individuals sighted at least three times and more than three times were measured by the half-weight index and SOCPROG program. The majority of these dolphins (80.57%) were not seen together during surveys and the mean association rate between dyads was low at 0.05. Temporal analyses for all the individuals were conducted using a lagged association rate. The results indicated a non-random social structure made by constant companions.

19

Radiofrequency ablation does not induce the significant increase of CD4+CD25+Foxp3+ regulatory T cells compared with surgical resection in Hepa 1-6 tumor model

76%

Gao H.-J., Zhang Y.-J., Liang H.-H., Li P., Peng Z.-W., Pang X.-H., Chen M.-S.

Archivum Immunologiae et Therapiae Experimentalis

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2013

|

tom 61

|

nr 4

20

Cell‑based immunofluorescence assay for screening the neurogenesis potential of new drugs in adult hippocampal neural progenitor cells

76%

Zhang K., Li B., Li P., Yang X., Cui H., Liu X.

Acta Neurobiologiae Experimentalis

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2019

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tom 79

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nr 3

Preclinical studies have suggested that increased adult neurogenesis in the hippocampus might have potential therapeutic effects for Alzheimer’s disease and depression; therefore, it is a target for the treatment of some brain diseases. In this technical communication, we propose a cell‑based fluorescence assay to study the neurogenesis of adult hippocampal progenitor cells that can be used for high‑throughput screening of drugs promoting neurogenesis. Three fluorescent dyes (DAPI, Alexa Fluor 488, and Alexa Fluor 594) and a fluorescence spectrophotometry reader were used, which confirmed that the mutual interference of the three fluorescent dyes is very low. We used this cell‑based fluorescence assay to evaluate the effects of three neurotrophic factors, ciliary neurotrophic factor (CNTF), insulin‑like growth factor 1 (IGF‑1), and IGF‑2 on the promotion of neurogenesis in adult hippocampal neural progenitor cells. The fluorescence intensity ratio of the neuronal marker, class III β‑tubulin, to the housekeeping protein, glyceraldehyde 3‑phosphate dehydrogenase, or nuclear staining dye, DAPI, in CNTF‑treated cells was significantly higher than in control cells. The ratios in IGF‑1‑ and IGF‑2‑treated cells were slightly higher under higher cell density conditions. These results are consistent with those in previous reports; therefore, this report proved the efficacy of this method. Taken together, the results showed that this simple, rapid, and economical cell‑based immunofluorescence assay could be a powerful tool for the rapid screening of drugs that promote adult neurogenesis.

Ograniczanie wyników

Effects of tillage alteration on soil water content, maize crop water potential and grain yield under subtropical humid climate conditions

Comparative proteomics analysis of young spikes of wheat in response to Fusarium graminearum infection

Perspectives on novel vaccine development

Relationship of changes in the fatty acid compositions and fruit softening in peach (Prunus persica L. Batsch)

Effects of Bothriochloa ischaemum characteristics induced by nitrogen addition on the process of slope runoff and sediment yield

Effectiveness of photochemical reflectance index to trace vertical and seasonal chlorophyll a/b ratio in Haloxylon ammodendron

First record of the genus Duriocoris Miller, 1940 [Heteroptera: Reduviidae: Stenopodainae] from China, with the description of a new species

Redescription of the little known reduviid, Tiarodes elegans Stal, 1863 [Heteroptera: Reduviidae: Reduviinae]

Aerodynamic performance of a new double-flap wing sail

Effects of solar ultraviolet radiation on photochemical efficiency of Chaetoceros curvisetus (Bacillariophyceae)

Temporal and spatial variations in soil TOC, TN, and TP, and their relationships in bioretention tanks

Comparison of salt tolerance between Cichorium intybus L. transformed with AtNHX1 or HvBADH1

Analysis of driving forces of energy consumption in rural areas of China’s Henan Province based on the STIRPAT model and ridge regression

Transformation of wheat Triticum aestivum with the HvBADH1 transgene from hulless barley improves salinity-stress tolerance

Chemical reduction of nitrate using nanoscale bimetallic iron/copper particles

Systematic assessment of reference genes for RT-qPCR across plant species under salt stress and drought stress

Drought stress induces oxidative stress and the antioxidant defense system in ascorbate-deficient vtc1 mutants of Arabidopsis thaliana

Sitefidelity and association patterns of Indo-Pacific humpback dolphins off the east coast of Zhanjing, China

Radiofrequency ablation does not induce the significant increase of CD4+CD25+Foxp3+ regulatory T cells compared with surgical resection in Hepa 1-6 tumor model

Cell‑based immunofluorescence assay for screening the neurogenesis potential of new drugs in adult hippocampal neural progenitor cells