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The role of enzyme amylase in two germinating seed morphs, i.e. black and brown, of Halopyrum mucronatum in saline and non-saline environment was examined. Both seed morphs of this halophytic grass have variations in their moisture content, total lipid, protein, sugar, phenol and tannin contents. Black seed exhibited higher activity compared to brown in saline medium. Sugar mobilization in both seed morphs was also affected due to the difference in amylase activity. However, exogenous application of GA₃ in saline medium enhanced the amylase activity and sugar mobilization. Phenolic contents were similar except for vanillic acid which was found only in brown seeds while catechol was present only in black seeds. Phenols extracted from both seed morphs were applied to determine their effects on amylase activity. Phenolic extracts obtained from brown seeds showed higher degree of inhibition of amylase activity. Results are discussed in relation to seed coat phenols, leaching, amylase activity and sugar mobilization.
Mulberry is grown worldwide. Its leaves are used to feed the silkworms which in turn produce silk fiber. Sericulture is mostly practiced in China, India and Japan. In the rest of the world, mulberry is generally used as forage in animal production, or for other purposes. Besides using the leaves, mulberry bears sweet fruit. The full-bodied flavor of this fruit is a good balance of sweetness and tartness with nutrient elements of vital importance for human metabolism. If these fruits are industrially exploited for various commercially valuable products, mulberry can become an important crop throughout the world. This paper presents a visualization of mulberry and the industrial exploitation of mulberry fruits for the global scenario. Mulberry can be used for making jam, jelly, pulp, fruit drink, fruit sauce, cake, fruit tea, fruit powder, fruit wine, food colorant, diabetes control agent and as ruminant livestock feed. It can also be used in the pharmaceutical industry. It opens a new vista for industrial exploitation of mulberry fruits worldwide. Such a use of the mulberry has been overlooked for the sake of using only mulberry leaf for the sericulture industry.
The effect of irradiated chitosan coating on post-harvest preservation of tomato was observed in this study. Irradiated chitosan (40 kGy) solution of various concentrations (500, 750, 1000, 1500 and 2000 ppm) were applied on post-harvest preservation of tomato. Both chitosan treated and untreated (control) tomato were stored at room temperature in open and zip bag conditions. The effect of coating of various chitosan solutions on tomato was observed during storage period. The percentage of weight loss and spoilage rate of the preserved and control tomato samples were investigated. Several parameters (such as total bacteria count, total mold count, moisture, ash, acidity, vitamin C, sugar, protein and fat) were analysed for irradiated chitosan coated tomato in open condition after 3-weeks storage period. In addition, the same parameters were also analysed for control tomato. Considering all parameters, the results revealed that 1500 ppm chitosan solution performed better in extending the shelf-life of tomato as compared to the control and other treated samples. Thus, this observation recommend that irradiated chitosan coating have the potential to be used as natural preservative to maintain quality and extending shelf-life of tomato.
Aqucous leaf extract of common lambsquarters (Chenopodium album L.) was evaluated alone or in combination with NaCl for its effect on germination and rice seedling growth. The treatments have no effect on germination. However, growth of both shoot and root significantly decreased under NaCl alone and in combination with leaf extract. Root growth was affected more than the shoot and the effect of NaCl was accentuated in the presence of leaf extract.
Suaeda fruticosa Forssk is a leaf succulent obligate halophyte that produces numerous seeds under saline conditions. Seeds are a good source of high quality edible oil and leaves are capable of removing substantial amount of salt from the saline soil besides many other economic usages. Little is known about the biochemical basis of salt tolerance in this species. We studied some biochemical responses of S. fruticosa to different exogenous treatments under non-saline (0 mM), moderate (300 mM) or high (600 mM) NaCl levels. Eight-weekold seedlings were sprayed twice a week with distilled water, hydrogen peroxide (H₂O₂, 100 µM), glycine betaine (GB, 10 mM), or ascorbic acid (AsA, 20 mM) for 30 days. At moderate (300 mM) NaCl, leaf Na⁺, Ca2⁺ and osmolality increased, along with unchanged ROS and antioxidant enzyme activities, possibly causing a better plant growth. Plants grew slowly at 600 mM NaCl to avoid leaf Na⁺ buildup relative to those at 300 mM NaCl. Exogenous application of distilled water and H₂O₂ improved ROS scavenging mechanisms, although growth was unaffected. ASA and GB alleviated salt-induced growth inhibition at 600 mM NaCl through enhancing the antioxidant defense system and osmotic and ion homeostasis, respectively.
In vivo modulation of HMG-CoA reductase (HMGR) activity and its impact on artemisinin biosynthesis as well as accumulation were studied through exogenous supply of labeled HMG-CoA (substrate), labeled MVA (the product), and mevinolin (the competitive inhibitor) using twigs of Artemisia annua L. plants collected at the preflowering stage. By increasing the concentration (2–16 µM) of HMG-CoA (3-¹⁴C), incorporation of labeled carbon into artemisinin was enhanced from 7.5 to 17.3 nmol (up to 130%). The incorporation of label (¹⁴C) into MVA and artemisinin was inhibited up to 87.5 and 82.9%, respectively, in the presence of 200 µM mevinolin in incubation medium containing 12 µM HMG-CoA (3-¹⁴C). Interestingly, by increasing the concentration of MVA (2-¹⁴C) from 2 to 18 µM, incorporation of label (¹⁴C) into artemisinin was enhanced from 10.5 to 35 nmol (up to 233%). When HMG-CoA (3-¹⁴C) concentration was increased from 12 to 28 µM in the presence of 150 µM mevinolin, the inhibitions in the incorporation of label (¹⁴C) into MVA and artemisinin were, however, reversed and the labels were found to approach their values in twigs fed with 12 µM HMG-CoA (3-¹⁴C) without mevinolin. In another experiment, 14.2% inhibition in artemisinin accumulation was observed in twigs in the presence of 175 µM fosmidomycin, the competitive inhibitor of 1-deoxy-D-xylulose 5-phosphate reductase (DXR). HMG-CoA reductase activity and artemisinin accumulation were also increased by 18.6 to 24.5% and 30.7 to 38.4%, respectively, after 12 h of treatment, when growth hormones IAA (100 ppm), GA₃ (100 ppm) and IAA + GA₃ (50 + 50 ppm) were sprayed on A. annua plants at the pre-flowering stage. The results obtained in this study, hence, demonstrate that the mevalonate pathway is the major contributor of carbon supply to artemisinin biosynthesis and HMGR limits artemisinin synthesis and its accumulation in A. annua plants.
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