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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The role of femtolaser in cataract surgery

100%
Nagy Z. Z., Szaflik J. P.
Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
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2014
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nr 2
2

Role of biochemical factors in the pathogenesis of keratoconus

81%
Wojcik K. A., Blasiak J., Szaflik J., Szaflik J. P.
Acta Biochimica Polonica
|
2014
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tom 61
|
nr 1
 Keratoconus (KC) is a corneal disease associated with structural abnormalities in the corneal epithelium, Bowman's layer and stroma and altered concentration of tear components. KC corneas show a different pattern of collagen lamellae than their normal counterparts. Also, a reduction of several collagen types in KC epithelium and stroma was observed. Altered expression and/or activity of lysyl oxidase, a critical enzyme of the biogenesis of connective tissue detected in KC corneas, may weaken covalent bonds between collagen and elastin fibrils, what may lead to biomechanical deterioration of the cornea. Increased activity of matrix metalloproteinases observed in KC may induce the degradation of the extracellular matrix causing damage to the cornea. Oxidative and nitrative stress play an important role in KC pathogenesis and KC corneas are characterized by the disturbed lipid peroxidation and nitric oxide pathways. Malfunctioning of these pathways may lead to accumulation of their toxic by-products inducing several detrimental effects, along with apoptosis of the corneal cells, which may result from the loss of β-actin or increased levels of cytokines, including interleukin-1 and -6. Change in the expression of genes associated with wound healing, including the nerve growth factor and the visual system homeobox 1, may contribute to increased susceptibility of KC corneas to injury. Consequently, biochemical changes may play an important role in KC pathophysiology and, therefore, can be considered in prevention, diagnosis, prognosis and in the therapy of this disease as well.
3

Pełzaki z rodzaju Acanthamoeba - czynniki etiologiczne stanów patologicznych ludzkiego organizmu

81%
Padzik M., Hendiger E. B., Szaflik J. P., Chomicz L.
Postępy Mikrobiologii
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2017
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tom 56
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nr 4
4

Keratitis incidences difficult to diagnose and treat suspected of amoebic aetiology in contact lens using patients

71%
Padzik M., Chomicz L., Szaflik J. P., Korwin M., Szaflik J.
Annals of Parasitology
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2013
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tom 59
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nr Suppl.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of Acanthamoeba keratitis – a growing threat to human health worldwide

71%
Padzik M., Baltaza W., Conn D. B., Szaflik J. P., Chomicz L.
Annals of Agricultural and Environmental Medicine
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2018
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tom 25
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nr 4
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Assessment of in vitro dynamics of pathogenic Acanthamoeba strains originating from contact lens wearers with infectious keratitis

61%
Padzik M., Starosciak B., Szaflik J. P., Pietruczuk- Padzik A., Siczek P., Chomicz L.
Annals of Parasitology
|
2016
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tom 62
|
nr 4
Recently, incidents of Acanthamoeba keratitis, the vision-threatening eye disease, are reported with increasing frequency worldwide, particularly in contact lens wearers. In our study, the retrospective assessment of in vitro dynamics of subsequent pathogenic Acanthamoeba isolates cultured at 24°C, detected in Polish contact lens wearers with keratitis is presented and results compared with those of environmental A. castellanii Neff strain. There were delayed the proper diagnosis that influenced prolonged and severe course of this eye disease and treatment difficulties. The corneal material was examined directly to visualize developmental amoeba stages for diagnose verification, microbiologically tested for the specific identification of bacteriae and fungi, and in vitro grown in culture medium in temperature 24°C. Among twenty-six keratitis incidents analyzed, eleven were cases of Acanthamoeba keratitis; in the six of them, Acanthamoeba strains and concomitant bacterial and/or fungal infectious agents were detected. In vitro assays showed variability in population density of several clinical strains in the exponential growth phase expressed in various range of overall amoeba number and different proportion between trophozoites and cysts. The clear influence of temperature on the in vitro cultivation of the amoebae was observed: statistically significant lower population dynamics was revealed by most of pathogenic clinical isolates in comparison with those showed by environmental strain. The in vitro monitoring of dynamics of Acanthamoeba strains isolated from infected eyes may be helpful for diagnostics verification, especially in mixed infectious keratitis.
7
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Evaluation of in vitro effects of selected physical and chemical agents on detected in Poland Acanthamoeba strains - factors of increasing threats for public health

61%
Baltaza W., Padzik M., Szaflik J. P., Perkowski K., Dybicz M., Chomicz L.
Annals of Parasitology
|
2019
|
tom 65
|
nr 1
Acanthamoeba keratitis, the vision-threatening corneal disease reported with increasing frequency in Poland is difficult to treat due to extremely high resistance of the amoeba cysts to chemicals. The agents of possible anti-amoebic activity are still tested. Pathogenic Acanthamoeba samples/isolates acquired from severe cases of keratitis examined by molecular techniques to determine genotypes, compared to one another as well as to the environmental Acanthamoeba castellanii Neff strain were included in the studies. These strains were in vitro examined in terms of their sensitivity/resistance to selected chemicals and tolerance to temperature changes. Samples of the strains cultivated in vitro under bacteria-free conditions were monitored during different growth phases. Higher amoebic population dynamics was observed in both pathogenic Acanthamoeba strains during transfer to 37°C. Agents tested influenced population dynamics in different degree; they showed amoebostatic or amoebicidal effects, however a tendency toward induction of encystment also appeared. Because activation of the dormant cysts can lead to repeated development of amoebae, very important is cysticidal efficacy of chemicals. Further in vitro investigations on various Acanthamoeba strains with different chemicals are still necessary.
8
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Amoebicidal or amoebostatic influence of disinfectants used in health facilities and laboratories on corneal strains of Acanthamoeba

61%
Baltaza W., Padzik M., Szaflik J. P., Dybicz M., Hendiger E., Chomicz L.
Annals of Parasitology
|
2017
|
tom 63
|
nr 3
Different Acanthamoeba species are amphizoic organisms distributed in wide range of habitats in natural and man-made environments; they are also detected on surfaces of equipment and accessories in health facilities. Some strains of the amoebae are causative agents of the vision-threatening human disease Acanthamoeba keratitis, mainly reported in contact lens wearers. An exceptional high resistance of Acanthamoeba trophozoites and particularly cysts to chemicals, disinfectants and drugs is believed as influencing difficulty resulting in unsuccessful therapeutic management. As Acanthamoeba keratitis is the serious medical problem worldwide, different chemicals with possible activity against environmental and clinical Acanthamoeba strains are tested. In our study, selected disinfectants used in health care settings and laboratories were tested and their efficacy against the corneal strains Acanthamoeba castellanii and A. polyphaga , and environmental A. castellanii Neff strain was assessed. Comparative assessment of results of the assays show that, apart from amoebistatic effects, the disinfectants indicated expected cysticidal efficacy.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Comparative examination on selected amphizoic amoebae in terms of their in vitro temperature tolerance - a possible indirect marker of potential pathogenicity of Acanthamoeba strains

61%
Baltaza W., Padzik M., Szaflik J. P., Dybicz M., Grochowska A., Kuryłowicz A., Chomicz L.
Annals of Parasitology
|
2018
|
tom 64
|
nr 4
Acanthamoeba species are ubiquitous in natural and man-made environments worldwide; some strains are able to colonize human eyes as facultative parasites. It has been shown that environmental and clinical isolates/species of Acanthamoeba vary in their pathogenicity. In this study we examine and compare the in vitro effects of the changing temperature on the population dynamics of subsequent amoebic strains. Identification of Acanthamoeba strain by morphological and molecular methods and temperature assays were performed. Monitoring of the corneal and environmental strains showed changes in population densities and a termo-tolerance correlating with pathogenicity of amoebae. Comparative assessment of results indicated differences in viability of amoebic populations in exponential growth phase in vitro cultivation. The increased awareness of the threat is needed for better understanding of impact of factors examined on pathogenesis in human infected with Acanthamoeba strains.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Comparison of chlorhexidine disinfectant in vitro effect on environmental and ocular Acanthamoeba strains, the amoebic agents of human keratitis - an emerging sight-threatening corneal disease in Poland

61%
Padzik M., Baltaza W., Szaflik J. P., Hendiger E., Dybicz M., Chomicz L.
Annals of Parasitology
|
2018
|
tom 64
|
nr 3
Small amoebae belonging to the Acanthamoeba genus complete their life cycles in different environmental niches as free-living protists however some of them are facultative parasites that can cause severe disease in humans. The sight-threatening Acanthamoeba keratitis develops in immune-competent persons, mainly in contact lens wearers; it is detected with increasing frequency along with the spread of contact lens use. The high abundance of the amoebae in the environment is important for dispersion and transmission of the infections among humans. Emerging threats for the public health generated by these amoebae is the serious medical problem worldwide. Nonspecific symptoms, similar to those occurring in the other eye diseases, diagnostic mistakes, the delay of an appropriate treatment, an exceptional high resistance of the amoebae to chemicals and drugs result in a prolonged course of the disease and often unsuccessful therapeutic management. Thus, different chemicals are still examined for their potential activity in vitro against various species, strains/isolates of Acanthamoeba. As the prolonged therapy often induces encystation subsequently leading to excystment and recurrences of amoebic keratitis, apart from anti-amoebic activity, cysticidal effect of examined agents is desirable. In the present study, results of our comparative investigations showed that cationic antiseptic chlorhexidine digluconate indicated in vitro anti-amoebic effect on environmental Acanthamoeba castellanii Neff strain and pathogenic corneal Acanthamoeba polyphaga T4 genotype. Amoebostatic effect of the disinfectant was expressed in reduced number of surviving amoebae in comparison to the respective control cultures; simultaneously, despite prolonged incubation with the agent no stimulation of encystation was noted. The corneal strain was more resistant to the tested compound than the Neff strain. The cysticidal efficacy of chemicals is very expected, thus further in vitro studies on pathogenic Acanthamoeba strains with different application chemicals pattern are needed.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In vivo confocal microscopy and in vitro culture techniques as tools for evaluation of severe Acanthamoeba keratitis incidents

61%
Padzik M., Szaflik J. P., Baltaza W., Perkowski K., Dybicz M., Chomicz L.
Annals of Parasitology
|
2017
|
tom 63
|
nr 4
Amphizoic amoebae belonging to the genus Acanthamoeba are known as etiological agents of sightthreatening Acanthamoeba keratitis. The leading risk factor for the development of this serious human disease is contact lens wearing which popularity increases worldwide, also in Poland. The disease with active epithelial inflammations, corneal ulcers, including loss of the visual acuity is a serious medical problem as an emerging threat for the public health related to improper contact lens hygiene. The treatment of the amoebic keratitis is difficult, often unsuccessful due to delayed proper diagnosis. The clinical picture of the disease, often with severe course is nonspecific, similar to that occurring in viral, fungal or bacterial keratitis, thus clinical symptoms alone are not sufficient to identify the causative agent of the amoebic infection. Early diagnosis is decisive for the suitable therapeutic management and the treatment efficacy. In our studies, several complicated, difficult to treat Acanthamoeba keratitis incidences pertaining Polish patients using contact lenses have been retrospectively analyzed in terms of the usefulness of non-invasive methods of in vivo confocal microscopy and in vitro culture techniques applied for diagnosis. Hyper-reflective double-walled spherical Acanthamoeba cysts, with a more reflective outer wall were detected in the epithelium and anterior layers of the corneal stroma. In vivo confocal microscopy, if available, may be a valuable, sensitive tool for diagnosis in late identified severe infections mainly with strong viability strains, however confoscan may offer limited value at lowintensity amoebic infections. The microscopic visualization of amoebae in slides prepared directly from corneal scraping and laboratory examinations of specimens from in vitro cultivated corneal isolates allow to confirm or verify results of in vivo examinations, furthermore to identify directly the pathogens and to clarify previous misdiagnoses.
12
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The first investigation on differences in the effectiveness of mycophenolate mofetil and azathioprine antimetabolites determined in Polish patients treated for non-infectious uveitis

61%
Waszczyk-Laczak M., Lazicka-Galecka M., Chomicz L., Mucha K., Paczek L., Szaflik J. P.
Annals of Agricultural and Environmental Medicine
|
2020
|
tom 27
|
nr 4
13

Polymorphism of the apex nuclease 1 gene in keratoconus and fuchs endothelial corneal dystrophy

51%
Wojcik K. A., Synowiec E., Kaminska A., Izdebska J., Polakowski P., Pawlowska E., Blasiak J., Szaflik J., Szaflik J. P.
Cellular and Molecular Biology Letters
|
2015
|
tom 20
|
nr 1
14

Transferrin receptor levels and polymorphism of its gene in age-related macular degeneration

51%
Wysokinski D., Danisz K., Pawlowska E., Dorecka M., Romaniuk D., Robaszkiewicz J., Szaflik M., Szaflik J., Blasiak J., Szaflik J. P.
Acta Biochimica Polonica
|
2015
|
tom 62
|
nr 2
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