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S. occidentalis can be used in pharmacology and vegetation restoration. A pot experiment was conducted to study the effects of biochar and mycorrhizal fungus inoculation on plant growth and heavy metal accumulation. The mycorrhizal infection rate was increased by apple branch biochar but decreased by coconut husk iron-modified biochar. Conversely, soil pH was not affected by mycorrhizal inoculation but was increased by biochar. Compared to the uninoculated control, the combination of apple branch biochar and mycorrhizal inoculation significantly increased the growth of stems, leaves and roots by 226.46%, 163.15% and 86.00%, respectively. The application of apple branch biochar increased the root Pb content, while root Cd, Cr, Cu and Fe were decreased by 36.30%, 13.63%, 3.09% and 7.66%, respectively. Furthermore, the content of all elements in the stems and leaves also decreased. The application of iron-modified biochar alone increased the content of all the elements in the roots by 4.23-109.33%. But their contents in stems and leaves were decreased by iron-modified biochar and mycorrhizal inoculation alone. The combination of biochar and mycorrhizal inoculation most effectively promoted plant growth, enhanced heavy metal uptake by the roots and produced a barrier effect that reduced the transfer of heavy metals from the roots to the shoots. This might constitute a feasible means of promoting the safe utilization of S. occidentalis in phytoremediation.
The effects of nitrogen (N) addition and increased precipitation on nitrous oxide (N2O) emissions in alpine meadow ecosystems are still unclear. In this study, we measured N2O fluxes on the Tibetan plateau under interactions of moderate atmospheric N deposition and increased precipitation using a closed chamber method. Under all applied treatment conditions, the alpine meadow ecosystem acted as a source of N2O. The N2O emission rate reached a maximum of 74.83±14.40 μg m-2 h-1, with a significant increase in emission rate of 68.76% following N addition when compared with the control plot (p<0.05). Increased precipitation, and its interactive combination with N deposition, enhanced the N2O emission rate by 53.90% and 44.52%, respectively. However, there was no significant difference between these two treatments. Increased precipitation would help to mitigate N2O fluxes under global nitrogen deposition conditions.
A polymorphism within exon 2 of the 2, 4-dienoyl-CoA reductase gene (DECR1) was investigated by PCR-SSCP in 228 Shanxi White pigs. An association between the DECR1 polymorphism and growth traits in Shanxi White pigs was determined with an univariate animal model. The polymorphism was found within exon 2 of the DECR1, giving rise to genotypes AA, BB or AB. This polymorphism exhibited a significant effect of generation and sex (P<0.05, P<0.01) on growth traits and backfat thickness. At the age of six months BB pigs showed the body weight and chest girth higher than AA and AB animals. However, the polymorphism revealed no significant effects on other growth traits (P>0.05) though a trend of BB>AB>AA was showed.
The mechanisms underlying the retention of inorganic N were still not well understood in an alpine meadow on the Tibetan Plateau as well as in other high-altitude meadow sites greatly grazed and disturbed. We conducted field soil and dominant species foliar nitrogen natural abundance of stable isotope ratios (δ¹⁵N) under four grazing intensities. It was demonstrated that soil δ¹⁵N decreased significantly from 5.83±0.20‰ to 2.17±0.48‰ at 0-10 cm with the elevation of grazing intensity. Grazing reduced the degree of ecosystem N openness. The δ¹⁵N value of surface soil was mainly affected by soil total nitrogen. Furthermore, the degree of nitrogen limitation increased with grazing elevation for sedge family and Gramineae family plants.
Acer buergerianum Miq. (Trident maple) is a native species of China with a large distribution, but exist in small population. Water and light are two important factors limiting plant growth and are crucial in the framework of forest regeneration. However, there is no consensus on how shade interacts with drought. Four hypotheses in the recent literature variously predict that shade will have a stronger, weaker or equal impact on seedlings under drought stress. This study investigated the interactive responses of A. buergerianum to light and water focusing on seedling growth, leaf morphology and biomass partitioning by performing a growth experiment in pots with different water supply regimes [15, 35, 55, 75, 95 % of field capacity (FC)] combined with two light regimes (10 and 66 % of full sunlight). After 123 days treatment, the results showed that shade greatly reduced growth and biomass, in contrast enhancing the amount of chlorophyll, the amount of water in the leaves, and the specific leaf area. Drought reduced growth, biomass, and the bulk of the leaves. Most leaf traits and biomass characteristics had strong interactions in their responses to light and water treatments. Allometric analysis revealed that water and light had no effects on root to shoot ratios, main root to lateral root ratios, and root mass ratios. Shade alleviated the negative impact of drought. A. buergerianum successfully adapted to the various light and water conditions. We recommend a water supply above 15 % FC to keep the seedlings vigorous, under both sunlight conditions.
In this study, we investigated responses of the mid-successional species Acer truncatum Bunge and the late-successional species Quercus variabilis Blume to three solar illumination conditions: (1) constant low light (CL), (2) constant high light (CH) and (3) low light first and high light afterwards (LH). The last treatment was to simulate a canopy opening. Both species exhibited increases in biomass, totally and in part, and decreases in leaf water content, specific leaf area and chlorophyll concentrations in LH treatment compared to CL treatment. For A. truncatum, exposure to high light condition (LH) increased crown area, and decreased root to shoot ratio, stem mass ratio and leaf perimeter. However, for Q. variabilis, LH treatment increased stem diameter at ground height, effective quantum yield, photochemical quenching and decreased maximum photosystem II quantum yield. The biomass allocation pattern did not change in Q. variabilis among three light conditions. With respect to newly developed leaves, no significant differences were found in leaf size of Q. variabilis between LH treatment and CH treatment while that of A. truncatum decreased in LH treatment. All chlorophyll fluorescence parameters in newly developed oak leaves in LH treatment increased compared to those of CH treatment while no difference was found for A. truncatum between LH and CH treatment. A. truncatum displayed a greater overall plasticity than Q. variabilis although the oak seedlings have a greater plasticity with respect to chlorophyll concentration and chlorophyll fluorescence parameters. A. truncatum should be a better candidate for vegetation recovery, especially in places with heterogeneous light conditions.
Elephants were confined to Mengyang Protected Area in China and their distribution range had reduced greatly compared to past records. A preliminary study of habitat selection by Asian elephantsElephas maximus Linnaeus, 1758 and their distribution was conducted in Mengyang Protected Area and its surrounds using site visits and transect surveys from July 2003 to December 2006. Although no variable significantly influenced their habitat selection, elephants still showed preference for altitudes between 900 and 1200 m, gradients <30°, and orientations to the south-east, south and south-west. Human activities, including habitat transformation and degradation, disturbance by large infrastructure and poaching were considered to be the main factors inducing elephant distribution changes.
Glucosinolates (GS) are a group of sulfur- and nitrogen-rich plant secondary metabolites that originate from amino acids and exist mainly in plants in the order Brassicales, such as Arabidopsis thaliana (Arabidopsis) and Chinese cabbage (Brassica rapa ssp. pekinensis). To date, several regulatory components responsible for GS biosynthesis have been identified in Arabidopsis. However, the functions of GS biosynthesis regulators in Chinese cabbage have not been clarified. In our current study, a putative ATR1/MYB34 orthologous gene, BrMYB34.2, was isolated from Chinese cabbage leaves. To investigate the function of this gene, we engineered Arabidopsis plants that overexpress BrMYB34.2 ectopically and phenotypic analysis was performed. Moreover, we assayed the accumulation levels of indolic GS (IGS) and aliphatic glucosinolates in transgenic plants and test the expression of key genes of IGS biosynthesis and tryptophan synthesis by Real-time quantitative PCR. And further analysed the resistance of transgenic plants in 5MT stress treatment. The results indicate that ectopic expression of the BrMYB34.2 gene in Arabidopsis was able to up-regulate the accumulation level of IGS due to the increased expression of IGS and Trp biosynthetic genes. Moreover, overexpression of BrMYB34.2 conferred Arabidopsis 5MT resistance. These results suggest that the BrMYB34.2 gene may function as one of the regulators of IGS and Trp biosynthesis in Chinese cabbage.
Degradation of shrub meadows and reclamation of alpine meadows may heavily affect the soil sink for atmospheric methane (CH₄), but this is poorly understood. Therefore, in situ measurements of atmospheric CH₄ consumption were conducted in four landuse types: natural alpine meadow (NM), Elymus nutans pasture (EP), herbaceous meadow in shrub (HS), and a P. fruticosa shrub meadow (PS) within two years. CH₄ fluxes were measured using static chambers and gas chromatography. All four types of land use showed atmospheric CH₄ sink throughout the two years, with mean soil CH₄ consumption rates at 24.6±10.9, 33.8±15.0, 39.8±10.3, and 28.1±12.1 µg CH₄·m⁻²·hr⁻¹ for NM, EP, PS, and HS, respectively. Soil CH₄ consumption increased by 40% by reclamation from NM to EP, while it decreased by 30% by degradation from PS to HS. Soil CH₄ consumption in four types of land use was significantly correlated with temperature at 5 cm depth (P<0.01) and the soil water-filled pore space (WFPS) (P<0.05). Temperature showed stronger effects on soil CH₄ consumption than WFPS, except in NM. UV radiation was positively correlated with soil CH₄ consumption with increasing temperature and decreasing soil moisture. These findings indicate that a decrease in the grazing pressure in shrub meadows and increase in the area of artificial pasture reclaimed from alpine meadows would enhance the CH₄ sink in alpine meadows on the Tibetan Plateau.
Propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy) benzylamino)benzoate (ZJ0273) is a new herbicide which inhibits acetolactate synthase (ALS). The ZJ0273 is considered as safe for the environment and exhibits a satisfactory effect on weed control in the rapeseed field. ALS is the key enzyme of reactions in the biosynthesis of total amino acids (TAAs) especially branched-chain amino acids (BCAAs). This study reports the effect of ZJ0273 on BCAAs and TAAs in rapeseed leaves using near-infrared spectroscopy (NIRS) techniques. A decrease in TAAs and BCAAs contents was observed as the herbicide dosages were increased along with leaf senescence. The wavelengths 2,416 and 1,340 nm were selected to develop the NIRS model for detecting BCAAs and TAAs, and correlation coefficients of model’s prediction set were 0.9823, 0.9764, 0.9831, and 0.9968 for valine, isoleucine, leucine, and TAAs, respectively. The results indicated that 100 mg/L ZJ0273 was a safe dosage for oilseed rape as it did not show a significant effect on the contents of amino acids compared to other higher dosages (500 and 1,000 mg/L).
Increasing levels of atmospheric nitrogen deposition have greatly affected forest trees. Acer truncatum Bunge has a large distribution in northern China, Korea and Japan and plays an important ecological role in forest ecosystems. We investigated the responses of A. truncatum to a broad range of nitrogen addition regimes with a focus on seedling growth, biomass partitioning, leaf morphology, gas exchange physiology and chlorophyll fluorescence physiology. Moderate nitrogen addition promoted shoot height, stem diameter at ground height, total biomass, size of leaves and chlorophyll fluorescence and gas exchange performance, whereas extreme level of nitrogen addition did not result in such facilitation. Chlorophyll content, pattern of biomass partitioning, ratio of leaf length to width, leaf water content, and specific leaf area did not change among the addition regimes. The critical amount of nitrogen deposition should be defined in the context of a certain time period in a particular region for a certain species at a special developmental stage. The critical amount of N deposition that weakens total biomass facilitation in A. truncatum planted in mixed soil of yellow cinnamon soil and humic soil is approximately 10 g N m−2 y−1 during the first growing season.
This study was aimed at qualifying the methane emission ability of different communities in alpine meadow, and monitoring if the dominant species from these communities could emit methane in a sand culture experiment. Using the static chamber technique and gas chromatography method, two experiments were conducted in the field and in laboratory. First, the methane flux rate was measured in plant communities: natural alpine meadows (NM), Elymus nutans pasture (EP), herbaceous community in shrub (HS), and a Poa fruticosa meadow (PS). A 3-month sand culture experiment was conducted to show the non-microbial methane emission from living plants. Average methane emission rates were estimated to be 16.83 µg m⁻² h⁻¹(range -49.3–107.8), 28.49 µg m⁻² h⁻¹ (range -55.0–96.2) and 20.91 µg m⁻² h⁻¹ (range -31.9– 145.8) for NM, EP, and PS, respectively. Methane emission rate from EP was significantly higher than from NM during the growing season. The reclaim of grassland would enhance the methane emission in this aera through this one year's measurement, but whether this conclusion suit to the whole Tibet Plateau, it remains further longer time and larger spatial scale experiments to verify it. The result of the sand culture experiment showed that some plant species emitted methane in an aerobic, nonmicrobial environment, most of herbaceous species showed a methane emission characteristic, the methane emission from plant may have a species dependent characteristic.
Ice cream was prepared with exopolysaccharide (EPS)-producing Lactobacillus plantarum YW11 by direct inoculation (DI), addition of prefermented skim milk (FSM), or addition of the lyophilized powder of the YW11 strain (LP) into the ice cream mix. After 4 weeks of storage, viable counts of the YW11 strain decreased in all groups by 0.8–1.61 log cfu/g. Furthermore, ice cream made using the LP method showed the highest survival rate. The ice cream processing and storage conditions also affected the YW11 strain’s tolerance to acid and bile, with a decrease in survival rate of 38.8–63.2% and 10.8–51.8%, respectively. The degree of impact on the viability of strain YW11 was hardening>aging>freezing>storage (p<0.05). The YW11 strain produced a ropy EPS (up to 4.84 mg/g) in the ice cream mix made using the DI and FSM methods; it was present as a fine porous matrix as observed by Cryo-SEM. Formation of the EPS together with changes in the pH of the ice cream mix caused increased viscosity (up to 131.0 mPa·s), overrun and meltdown, decreased destabilization of fat, and firmness of ice cream. Hydrocarbons, ketones, and benzenes were found to be the major volatiles in the fermented ice cream samples, which also had decreased levels of dodecane, characterized by the smell of dirt.
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