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The subject of investigations was the evaluation of sanitary-hygienic state of slurry subject to anaerobic fermentation in bioreactor under thermophilic conditions. The influence of anaerobic fermentation on the survival of Salmonella senftenberg, EHEC and streptococci D group was specified. It turned out from investigations that after 4 hours the number of Salmonella senftenberg and EHEC bacteria decreased 108 times in relation to the control test. The lowest elimination rate was for streptococci D group. The percentage of invasive eggs of Ascaris suum decreased after 4 hours from 89% to 7%. The investigation proved proper run of anaerobic process in bioreactor and sufficient desinfection of cattle slurry.
Microbiological and parasitologic investigations dealing with the influence of aeration on fecal bacteria and Ascaris suum eggs survival in cattle slurry were carried out. The results showed that already the first stage of aeration (reactor I) eliminated quickly a number of Salmonella senftenberg (105 times during 2.5 hours in relation to the control test) and EHEC (108 times during 2.5 hours in relation to the control test) respectively. The longest survival time was for D group streptococci. Inactivation of Ascaris suum eggs due to thermic conditions fluctuated between 50 and 90 minutes. The final stage of aeration (reactor II) completely destroyed pathogen germs and Ascaris suum eggs present in cattle slurry.
Microbiological research on bioaerosols was carried out at the Municipal Sewage Treatment Plant in Toruń. The number of staphylococci in the atmospheric air was determined at grit chambers, aeration tanks, and in the vicinity of maturing compost piles. On the basis of Polish Standards, a medium air pollution with staphylococci was reported at the object, the majority of them occurring in the air around the compost piles in summer. The antibiotic-resistance of the 42 tested strains of Staphylococcus spp. showed the greatest sensitivity to metacycline (59.6%) and then to erythromycin and vancomycine (40.5%). Novobiocine, to which 66.7% resistant strains were found, appeared to be the least effective antibiotic.
Animal by-products of category 3 after proper processing may be a valuable fertilizer for agricultural purposes. However, they can contain numerous bacterial and viral pathogens and, in cases of improper handling, may pose a health risk for people and animals. This study is aimed at monitoring the number of fecal streptococci introduced into carriers of different types imitating fragments of meat and bone wastes during composting process in a drum bio-reactor. Fecal streptococci are indicator microorganisms, and are known by their thermoresistant characteristics, so it was assumed that their elimination will also diminish the pathogenic microorganisms present in wastes. Three research cycles were carried out in a drum-type bio-reactor, and a different course of temperature was noted in each of them. In cycle 1, in which the temperature exceeded 60ºC, fecal streptococci died the fastest, 139.0-154.4 hours later (depending on carrier type). In cycles 2 and 3, maximum temperatures were similar (57.2ºC and 58.8ºC, respectively), but secondary multiplication of the streptococci in the 102nd hour of the processes was observed. In cycle 2 at this time their number was similar to the level of initial suspension. The type and size of the carriers were of no major importance to streptococci survival in the bio-reactor. Yet in each of the cycles analyzed, effective reduction was accomplished and the product obtained can be considered to be environmentally safe.
The research was carried out on farms in Kiełpin in Bory Tucholskie Landscape Park in vicinity of Tuchola over 1997-1999. The purpose was to determine of quantitative composition of Pseudomonas and Arthrobacter genera, coryneform group and total count of bacteria on roots of potato. Aster cultivar of potato was cultivated in two systems: conventional and ecological. The microbiological analysis showed that the coryneform group dominated over other investigated groups of bacteria. The greatest number of bacteria was isolated from rhizop1ane of potatoes cultivated in ecological system. The results obtained during three years of the experiment show that the number of potentially antagonistic bacteria increased along with plant vegetation. The maximum number of these bacteria inhabited roots of older plants.
Przeprowadzono ocenę skuteczności oczyszczania ścieków z zakładu mięsnego na podstawie określania ogólnej liczby drobnoustrojów oraz pałeczek E. coli, Salmonella i paciorkowców grupy D w ścieku surowym i oczyszczonym. Badania wykazały, iż skuteczność oczyszczalni mechaniczno-chemicznej pod względem usuwania zanieczyszczeń mikrobiologicznych jest niewielka. Liczba badanych bakterii tylko nieznacznie obniżała się po oczyszczeniu, bez względu na porę roku. Szczególnie niepokojący był fakt częstego wykrywania w oczyszczonym ścieku pałeczek Salmonella. Przeprowadzone badania wskazują na konieczność poddania ścieków z zakładów mięsnych dalszemu oczyszczaniu metodami biologicznymi.
Microbiological research of bioaerosols was carried out at the Municipal Sewage Treatment Plant in Toruń. The concentration of selected bacteria, fungi and actinomycetes in the atmospheric air was estimated in the vicinity of sand catchers, aeration chambers and maturing compost piles, as well as 100 m beyond the treatment plant. It was found that the air at the test stands showed different degrees of microbiological pollution. The largest bioaerosol emission sources were the sand catcher and the maturing compost storage facility. The total number of bacteria and fungi amounted to a maximum of 104 CFU m-3 and of actinomy­cetes - 103 CFU m-3. The bacteria of the genus Pseudomonas (fluorescent subgroup) occurred at all the stands throughout the study except December. The number of Escherichia coli and bacteria of the genera Enterococcus and Salmonella remained at the very low level of about 10'CFU m-3, and of all these bacteria only fecal streptococci D-type were isolated at stand 4 (beyond the treatment plant). On the basis of the research carried out and the results obtained at the control stand one may conclude that the test facility does not pose a hazard in respect of the tested bacteria emission.
The aim of the study was to evaluate a method of animal by-product disposal based on a significant increase in pH caused by the addition of quicklime. Three different doses of CaO were tested - 10%, 20%, or 25% (expressed in dry matter). The inactivation rate of indicator bacteria - Escherichia coli and faecal streptococci was the main criterion of the estimation of the process efficiency. The maximum temperature value generated inside the biomass of the 25% share of lime was 37.7°C. Addition of each CaO dose caused an increase in pH over 12. Application of the highest amount of CaO (25%) resulted in an inactivation of coliform rods within 3 h. Under similar conditions, faecal streptococci survived more than 4 h. A dose of 10% caused a rapid decline of E. coli in meat for 6 h. Streptococci were still isolated from the same samples after 24 h of liming.
The aim of this study was to examine the rate of Ascaris suum egg inactivation in meat wastes subjected to different sanitisation technologies. The wastes were composted in a drum bioreactor and fermented under mesophilic (37°C) and thermophilic (55°C) conditions. A suspension with fertilised eggs of A. suum was introduced into perlon sacks and placed in waste biomass. They were removed at fixed-time intervals and the percentage of live eggs was calculated. The eggs were inactivated the earliest during thermophilic fermentation and their total time of survival amounted to approximately 10 h. The full inactivation of eggs during composting was obtained after 72 h, while in the course of mesophilic fermentation. No live eggs were detected on 14th d. All the sanitisation technologies analysed ensured the environmental safety of product obtained.
The aim of the study was to evaluate the inactivation of Ascaris suum eggs during composting waste from municipal green areas with the addition of sewage sludge from Kneer container technology. Carriers with parasite eggs were placed in the top, medium and bottom layers of the composted biomass. Analyses showed distinct differences in egg inactivation in particular biomass layers. Ascaris suum eggs survived longest at the bottom - from 3 days in the summer cycle to 161 days in the spring cycle. The egg’s elimination rate in the summer cycle was remarkably higher than in the spring. No eggs able to develop further were detected at all the tested layers of the biomass after 4 days, while in spring the survival rate based on a calculation regression lines ranged from 20 to 161 days.
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