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The influence of pre-sowing seed treatments on germination, seedling growth, ion accumulation and plant antioxidant system in spring wheat (Triticum aestivum L.) cultivar MH-97 was examined under control and saline conditions. Albeit, all pre-sowing seed treatments were effective in improving seedling fresh and dry weight under saline conditions, the effect of hormonal priming with kinetin was very pronounced particularly in improving seedling vigor. The plants raised from seeds primed with kinetin have maximum root and shoot lengths under saline conditions while pre-sowing chilling treatment increased shoot length maximally whereas halopriming increased root length maximally under non-saline conditions. The Na⁺ uptake was decreased while K⁺ uptake was increased in plants due to hormonal priming with kinetin and hydropriming under stress conditions. Except halopriming, all seed treatments significantly increased leaf protein contents under non-saline conditions, however decreased under saline conditions. Halopriming resulted in a significant increase in protein concentration. Catalase (CAT) activity was significantly increased under salinity stress, however, hormonal priming and halopriming treatments maximally increased CAT activity. All the seed treatments were effective in increasing superoxide dismutase (SOD) level but hormonal priming with kinetin maximally increased its activity under stress conditions. Although all pre-sowing seed treatments were effective in alleviating the adverse effect of the salt stress on wheat plants, their effects on altering the concentration of different ions, seedling growth and plant antioxidant system were different in the wheat plants.
A pot study was conducted in net house to determine the influence of hormonal priming kinetin (50 mg·dm-3), salicylic acid (50 mg·dm-3), spermine (50 mg·dm-3) and spermidine (50 mg·dm-3) on emergence, yield, antioxidants and ionic content of two wheat (Triticum aestivum L.) cultivars SARC-1 (salt tolerant) and MH-97 (salt sensitive) under normal and saline conditions. The growth of wheat plants was severely affected by salinity stress. But the most of hormonal priming strategies were effective in alleviating the adverse effects of salt stress on wheat plants, however, hormonal priming with salicylic acid (SA) followed by priming with kinetin successfully enhanced seedling vigor by increasing emergence index, decreasing the uptake of Na+ and finally increasing grain yield of both cultivars under normal as well as saline conditions. Activities of SOD and CAT in both cultivars were significantly affected by priming in stressful environment. On the other hand, SARC-1 performed better as compared to MH-97 due to the increased antioxidant activities under salt stress. It can be concluded that priming with 50 mg·dm-3 SA followed by 50 mg·dm-3 kinetin for 12 h maximally improved salt tolerance in both wheat cultivars.
Poor plant stand establishment is a common problem for marigold production. Exposure to seed priming is one of the strategies being used to accelerate the activation of plant growth, especially seed germination in many crops. Therefore, an experiment was conducted to investigate the influence of different seed priming techniques on performance of French marigold. For this purpose, different seed priming techniques: hydroprimng, 50 mM CaCl2, 100 mg L⁻¹ salicylic acid and 100 mg L⁻¹ ascorbic acid were assessed. Seeds without any treatment were considered as control. Priming with 50 mM CaCl2 was proved to be the most effective treatment in enhancing germination and seedling vigour among all other treatments including control as shown by higher final germination/emergence, germination energy and lower mean germination/emergence time. Furthermore, positive correlation between reducing sugars and dry weight and α-amylase activity and the concentration of reducing sugars indicates that enhancement due to priming could be attributed to higher reducing and total sugars as well as higher α-amylase activity in primed seeds.
Among the natural plant growth stimulants, moringa has attained enormous attention due to its leaf composition being enriched with cytokinin, antioxidants and minerals. Exogenous application of moringa leaf extract (MLE) improves productivity in many crops. This study investigated the potential of MLE with different dilutions, i.e., MLE0, MLE10, MLE20 and MLE30 (0, 10, 20 and 30 times diluted in water, respectively) to improve the performance of tomato. Foliage-applied water and benzylaminopurine (BAP, 50 mg L⁻¹) were taken as controls. Among treatments, foliar-applied MLE30 produced maximum vegetative and flowering branches, number of flowers and heaviest fruits per plant of tomato in comparison with synthetic BAP and other treatments. A similar increase in vegetative and flowering branches was recorded for root-applied MLE20 including BAP. Foliageapplied MLE30 also increased chlorophyll (a) pigments and leaf total soluble proteins than other stimulants used. This increase was followed by enhanced antioxidant activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total phenolics in leaves and fruit lycopene contents of tomato. In general, foliar application of MLE30 was more effective as natural biostimulant to improve growth, productivity and fruit quality of tomato as compared to synthetic BAP and its root application.
Sulphur (S) nutrition is very important for harvesting potential seed and oil yield of rapeseed. This study evaluated response of foliage applied thiourea on the performance of two canola cultivars Shiralee and Dunkeld. Sulphur was applied to soil (40 kg ha-1) or foliage (500 and 1,000 mg L-1) at rosette, bud initiation and flowering stages using elemental S or thiourea as source, respectively; no S application was taken as control. Among all the treatments, soil application of S improved the crop growth, yield and oil quality in both cultivars and was followed by foliar application of thiourea at 1,000 mg L-1 compared with no application. Soil applied S and foliar thiourea (1,000 mg L-1) delayed the flowering and maturity. Soil and foliar applied S significantly improved leaf area index, crop growth rate, net assimilation rate and chlorophyll contents. Plant height, number of branches, siliqua per plant, seed number per siliqua, 1,000-seed weight, biological and seed yield were also increased by soil applied S and foliage applied thiourea (1,000 mg L-1). Nonetheless, improvement in harvest index, seed oil, protein and glucosinolate contents was only observed from foliage applied thiourea (1,000 mg L-1). Response of cv. Shiralee to sulphur application was better than cv. Dunkeld. In conclusion, foliar applied thiourea (1,000 mg L-1) can have potential to improve growth, yield and oil quality in canola and can be economically viable and attractive alternative source.
Tomatoes reportedly have a positive response to seed priming. The present study evaluates the effects of ethanol priming on germination, seedling vigour and antioxidative responses of tomato seeds. Priming was achieved by exposing seeds of ‘Roma’ and ‘Nagina’ to 2, 4 and 6% aerated ethanol solutions for 24 h. Priming with low levels (2 and 4%) of ethanol improved seed germination, seedling vigour and enhanced antioxidative activity that results in better performance of tomato seeds. However, priming with 6% ethanol failed to improve seed germination and seedling development which relates to the decreased anti-oxidative activity in tomato seeds due to high ethanol level.
Tomato is well known regarding its quality and nutritional value in all over the world but imbalances of fertilizer nutrients severely affect the quality of tomato. To investigate the specific contribution of potassium to yield and quality of tomato, a field experiment was conducted on two tomato cultivars, Nagina and Roma. Foliar application with varying levels (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0%) of potassium solutions was applied to the plants and compared with control (without K). Exogenous application of 0.6% K significantly improved plant height, lycopene content, potassium, fruit weight and diameter. Exogenous application of 0.5, 0.6 and 0.7% K maximally improved ascorbic acid contents of both tomato cultivars whereas 0.4 and 0.8% did not improve ascorbic acid contents. Due to positive correlation between K nutrition and fruit quality attributes, exogenous application of an appropriate K level can contribute to higher yield and better quality of tomato fruits. Among all potassium levels, 0.5–0.7% K maximally improved performance of tomato plants of both cultivars.
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