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To supply more documentary evidence for the effect of parasitic infection on activity of neurosecretory centres in the cerebral ganglia of the snail host, caudo-dorsal and light green cells of adult individuals of Lymnaea stagnalis naturally infected with digenean parthenites were subjected to karyometric analysis. In infected animals significant enlargement of nuclear volumes of both examined types of neurosecretory cells was ascertained. Mean nuclear volume of the light green cells in snails infected with Furcocercariae was significantly lower in comparison with the individuals infected with parthenites from groups Echinostomata and Xiphidiocercariae. In remaining cases differences in nuclear volumes among subpopulations distinguished on the ground of an infection factor were not statistically significant.
Qualities of microscopical pictures of digenean larvae total slides stained with different acetocarmines were compared. Semichon's acidifying carmine for topographical staining. Schneider's and Belling's carmines for chromosomes as well as iron acetocarmine (modification of the last two) were used. The last stain permits to obtain the slides, that in respect of their colour and quality of microscopical picture may be compared with slides stained with Semichon's carmine. Contrast, colour and reaction of staining depend on concentration of iron acetate in the staining solution. Total slides of investigated larvae stained respectively with Schneider's and Belling's carmines are not enough readable.
On the background of informations about frequency of appearance Fasciola hepatica L. among cattle in Poland im the time from 1995-1997, as well es in the former period, there is presented in the article an estimative apperiation of economical losses caused by staggers disease among cattle. The highest extensiveness of invasion of F. hepatica L. among cattle was noticed in the fifties and sixties manty in the north-eastern Poland. Losses caused by confiscation of a liver to amounted to 1197031zł in 1995, 1277154zł in 1996 and 1312078zł in 1997.
Cases of taeniosis were mostly caused by Taenia saginata (318 cases – 84.8%). They were more frequently found in urban populations (255 cases, which is 68%) than in villagers (63 cases - 16.80%). Among the studied subjects there were fewer women than men (women - 147, 39.2%, men - 171 - 46.67%); taeniosis was diagnosed most frequently among persons aged 30-39 (89 cases - 23.73%) and only rarely in children aged 0-9 (8 cases – 2.13%). From 1986 through 1994 cysticercosis caused by Cysticercus bovis was found in 1684 (0.018%) animals of 934202 heads of cattle slaughtered in Katowice district. That particular cysticercosis was observed each year. During the period 1986-1994 cysticercosis caused by Cysticercus cellulosae was found in only few pigs (6 cases - 0.0001 % of 5930961 examined pigs). In the same period of time echinococcosis was found in 196711 pigs, which is 3.3% of 5930961 examined animals.
The autors present an extensiveness of invasion of hydatidosis among swine in Poland. It is ascertained that a hydatidosis among swine appeared most often in the Eastern Poland. The analysis of these data shows that the percent infected animals, the highest extensiveness of invasion hydatidosis among swine occur in the districts Zamość, Łomża and Chełm.
Acid and neutral lipids in epithelial cells building tubules of the digestive gland of snails naturally infected with digenean larvae were stained with nil blue sulphate. Strong decrease in number of phospholipids granules in secretory cells, as well as in calcium cells in the infected glands was observed. The larvae caused also decrease of number of neutral lipids in both types of cells, but these changes were much smaller and did not refer to small granules laying in top parts of the epithelial cells. Changes in number of lipids granules were limited only to the cells of tubules damaged by parasites.
Total protein and activities of: alpha-amylase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase in haemolymph of the freshwater snail Lymnaea stagnalis, naturally infected with digenean larvae were investigated. There were no any changes in these parameters in the snails infected with Cercaria tenuispina that occupies mainly haemocoel sinuses. Significant increase of activities of all examined enzymes in haemolymph of the snails infected with larvae belonging to the Furcocercariae group was ascertained. These changes were proportional to the degree of injury of the digestive gland, examined with the use of the thymol turbidity test. Total protein level was significantly higher only in haemolymph of snails with higher values of this test.
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