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In this study, biochar (BC), triple superphosphate (TSP), and TSP+BC amendments were utilized for remediation of Cd, Pb, and Cu co-contaminated agricultural soils. The toxicity characteristic leaching procedure (TCLP), the European Community Bureau of Reference (BCR), X-ray diffraction, and scanning electron microscopy-energy dispersive spectrometer techniques were employed to evaluate the effectiveness of the three types of amendments. After soil amendment, pH, heavy metal concentrations in TCLP extracts, and BCR speciation of heavy metals showed significant changes. The application of BC, TSP, and TSP+BC to co-contaminated soils slightly increased soil pH; decreased Pb, and Cu leachability in the TCLP extracts; and lowered the concentrations of the acid-soluble fraction of heavy metals. The application of TSP+BC mixture at the same dose as BC and TSP produced the greatest reduction in available heavy metal concentration. The optimum mass ratio of TSP to BC was 1:3. Overall, the TSP+BC mixture was highly effective in immobilizing Cd, Pb, and Cu in co-contaminated agricultural soils. The experimental results demonstrate that the rational application of the TSP and BC provides benefits of retrenching phosphorus resources, decreasing phosphorus pollution, and lowering the feed costs of debasing soil remediation treatments.
The bearing response of pile foundations for cross-sea bridge subjected to lateral loading is investigated through three-dimensional finite element numerical analyses. In the analyses, non-linear behavior of concrete is simulated using smeared cracking model, and the strain-stress relationship of rebar is modeled through perfectly elasto-plastic model obeying Mises yield criterion. The finite element model is validated against published lateral static loading test in situ. The effect of reinforcement ratio of reinforced concrete and vertical load level is explored on the displacement of pile head and lateral capacity of pile. The results show that for the pile with low reinforcement ratio, the allowable lateral capacity is controlled by concrete cracking, however the allowable lateral capacity is controlled by the displacement of pile head with high reinforcement ratio. The vertical load applied on the pile head may reduce its displacement but increase simultaneously the maximum moment in the pile body. Therefore, the optimum vertical load level is 0.4~0.6 times of the vertical ultimate load of a single pile
This in-depth paper studies the issue of energy-related CO₂ emissions of China using sample data from 1980 to 2015. Due to the lack of official data, CO₂ emissions are first calculated by the recommended IPCC method. It shows that CO₂ emissions in China present an “S” type in shape. Then the Tapio decoupling index is applied to investigate the relationship between CO₂ emissions and economic growth. This suggests that weak decoupling is the main state during the study period and the decoupling trend is M-shaped. Moreover, the study years are divided into decoupling years and re-link years according to the decoupling relationship, and the ReliefF algorithm is proposed to verify the feasibility of the classification and judge the influencing weights of different driving factors. The ascending order is: actual GDP, urbanization rate, industrial structure, population, energy structure, and electricity consumption. Finally, a hybrid model of grey neural network model (GNNM) based on grey model (GM) and BP neural network (BPNN) is established to forecast CO₂ emissions. This demonstrates that the GNNM model has a better capacity for forecasting CO₂ emissions and capturing the non-linear and non-stationary characteristics of CO₂ emissions.
Groundwater is considered a major source of drinking water and its quality a basis for good population health. In order to identify groundwater hydro-chemical characteristics and pollution conditions in Songnen Plain, groundwater hydro-chemical characteristics and nitrate-nitrogen (NO3-N) spatial distribution characteristics and the health risks were analyzed. Results showed that groundwater hydro-chemical type was mainly HCO3-Ca, which was associated with the action of calcite and silicate mineral weathering dissolution. The over standards rate of NO3-N accounted for 50.8%, the coeffi cient of variation was 183.57% which was high spatial variability, the high-risk area accounted for 88.78% of the total study area, and the high-risk area covered the area with water quality of classes IV, V, and part of class III. The high-risk area is mainly distributed in the eastern high plains and in the central low plains, while the low-risk zone accounts for only 11.22% of the total area and is mainly distributed in the western alluvial plain with scattered distribution in other areas.
To investigate the physiological mechanisms of drought stress mitigated by exogenous cinnamic acid (CA), cucumber seedlings were pretreated with 50 µM CA for 2 days and then were subjected to 10% polyethylene glycol (PEG) 6000. We examined if CA protects plants from PEGinduced drought stress, and whether the protective effect is related to antioxidant and lipid-peroxidation regulation. 2 days of CA application enhanced the activities of guaiacol peroxidase (GPX), glutathione peroxidase (GSH-Px), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) and increased the levels of ascorbate, proline, soluble sugar, vanillic acid (VA) and CA in leaves. After CA-pretreated leaves were exposed to drought, the activities of superoxide dismutase (SOD), catalase, ascorbate peroxidase, monodehydroascorbate reductase, GPX, GSH-Px, DHAR and GR were higher than under drought stress alone, while the levels of reduced glutathione, ascorbate, proline, soluble sugar,VAand CA in leaves were more. The combination of exogenous CA and drought led to higher transcript levels of Cu/Zn-SOD and Mn-SOD genes and decreased contents of malonaldehyde and hydrogen peroxide, but drought had adverse effects on them. Furthermore, the combined effects of exogenous CA and drought made 61.67% leaf edges dried, while drought resulted in 95.83% withered leaves. We conclude CA pretreatment leads to higher contents of CA and VA in drought-stressed leaves and thereby results in higher antioxidant activities directly or indirectly via proline and soluble sugar, thus increasing drought tolerance of cucumber. We also propose soluble sugar can reduce reactive oxygen species and decrease lipid peroxidation when exogenous CA mitigates drought stress.
Focusing on the variations of chemical constituents in licorice root, influences of exposure to physical factors of spaceflight on licorice (Glycyrrhiza uralensis Fisch.) seeds were investigated. Licorice seeds obtained from two different producing areas were flown on a recoverable satellite for 18 days. After returning to earth, the seeds carried by the satellite and the parallel ground control were cultivated to maturity under the same condition. Chromatographic fingerprint of 1 year licorice root analyzed by high performance liquid chromatography with diode-array detection not only displayed the contents of glycyrrhizic acid and liquiritin increasing in the spaceflight samples but showed the variation of the kinds of chemical constituents. The main components in the root extract were identified by high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry. The changes in the kind of secondary metabolites of licorice root after spaceflight were firstly reported. A total of 26 components which included 9 flavonoids, 16 triterpene saponins and 1 coumarin were identified according to their mass spectra determined in both negative and positive ion modes. The research provided the scientific data for spaceflight breeding of medicinal plant and indicated that the technology of spaceflight may be a new effective method for the breeding and cultivation of licorice.
Small heat shock proteins (sHSPs) are the critical components of responses to various environmental stresses. However, few have been functionally characterised in Primula. In this study, we cloned a sHSP gene, PfHSP17.1, which is highly up-regulated in the leaves of Primula forrestii exposed to thermal stress (42 °C for 2 h). Sequence alignment and phylogenetic analysis indicated that PfHSP17.1 is a member of the plant cytosolic class I sHSPs. This gene was basally and ubiquitously expressed in different plant organs. The expression of PfHSP17.1 was also triggered remarkably by salt, drought and oxidative stress conditions but was only slightly induced by abscisic acid. Transgenic Arabidopsis thaliana constitutively expressing PfHSP17.1 displayed increased thermotolerance and higher resistance to salt and drought compared with wild-type plants. These results highlight the important role that PfHSP17.1 plays in diverse physiological and biochemical processes related to adverse conditions.
Although the treatment for recycling chrome in tanning effluents is relatively mature, the post-tanning processes have no corresponding effective technology due to the lack of knowledge about the effluents. In this work we investigated the composition and properties of rewetting and neutralizing effluents in posttanning processes. Goat skins were tanned with sulfate chrome liquor of alkalinity 33% (SC), and then conventional technologies were applied to get rewetting and neutralizing effluents (RE, NE). The effluents were separated and analyzed by ion exchange chromatography, gel filtration chromatography, ultravioletvisible absorption spectroscopy, and Fourier-transform infrared spectroscopy. Hide powder tanned by the chrome recovered from the effluents was evaluated by thermogravimetric analysis and differential scanning calorimetry. The results indicated that RE and NE showed different valent chrome complexes from SC. Released chrome mainly combined with SO₄²⁻ and HCOO⁻ through a single-point coordinate mode. Molecular weight of chrome complexes in the effluents was smaller and the tanning ability was poorer. Based on the results, the composition and properties of chrome complexes in RE and NE were understood, and some of their structures were speculated. Since these characteristics have a relationship with tanning performance, they may provide theoretical support for developing effluent-related recycling technology in the leather industry.
This study was conducted to examine the seasonal dynamics of biomass and plant nitrogen (N) content under three grazing intensities (light grazing – LG: 1.2, moderate grazing – MG: 2.0, and heavy grazing – HG: 2.9 yaks ha⁻¹) in representative alpine meadow on the eastern Tibetan Plateau. Differentiation in grazing intensity in the study area started since 1997 and has continued to the present time. Plant samples were collected in the middle of June, August and September. The highest aboveground biomass occurred at the MG site for both August and September. Over the growing season, belowground biomass (0–30 cm) increased as grazing intensity increased. The total belowground biomass averaged over all sampling dates was 1226, 1908 and 2244 g m⁻² for LG site, MG site and HG site, which accounted for 75, 81 and 88% of total biomass, respectively. The results suggested that grazing intensity changed biomass allocation pattern between aboveground and belowground parts of plants. Higher grazing intensity resulted in higher N concentration in both live and dead aboveground biomass over the study period. Increased grazing intensity tended to increase plant N content averaged over all sampling dates, which were 17.9 g m⁻², 23.8 g m⁻² and 27.6 g m⁻² in LG site, MG site and HG site. The results indicated that higher grazing intensity had a potential to increase the ecosystem pool of plant N.
The aim of this study was to evaluate the effects of different light quality of light emitting diode (LED) on the growth, concentration of chlorophyll and chlorophyll biosynthesis precursors of non-heading Chinese cabbage (Brassica campestris L.). Seedlings of the cultivar Te Ai Qing were cultured for 28 days under 6 treatments: red light (R), blue light (B), green light (G), yellow light (Y), red plus blue light (RB) and dysprosium lamp (CK). Lighting experiments were performed under controlled conditions (photon flux density 150 μmol m-2 s-1; 12 h photoperiod; 18–20 º C). The fresh and dry mass were the greatest under RB, which were significantly higher than other light treatments. The fresh mass under RB was almost twice higher compared to other light treatments. Plant height was highest underRtreatment and was lowest under B.RBtreatment also lowered the plant height significantly. The highest soluble sugar concentration was observed under B. The soluble protein concentration was the greatest under RB. The R treatment was adverse to pigment accumulation. The concentration of photosynthetic pigments and chlorophyll biosynthesis precursors were higher under RB. The RB treatment was beneficial to pigment accumulation.
Crosstalk in wiring harness has been studied extensively for its importance in the naval ships electromagnetic compatibility field. An effective and high-efficiency method is proposed in this paper for analyzing Statistical Characteristics of crosstalk in wiring harness with random variation of position based on Polynomial Chaos Expansion (PCE). A typical 14-cable wiring harness was simulated as the object of research. Distance among interfering cable, affected cable and GND is synthesized and analyzed in both frequency domain and time domain. The model of naval ships wiring harness distribution parameter was established by utilizing Legendre orthogonal polynomials as basis functions along with prediction model of statistical characters. Detailed mean value, mean square error, probability density function and reasonable varying range of crosstalk in naval ships wiring harness are described in both time domain and frequency domain. Numerical experiment proves that the method proposed in this paper, not only has good consistency with the MC method can be applied in the naval ships EMC research field to provide theoretical support for guaranteeing safety, but also has better time-efficiency than the MC method. Therefore, the Polynomial Chaos Expansion method
The application of nitrogen fertilizers can improve the soil environment and influence the nutrition and quality of plants; there are also significant impacts of soil types on soil microbial communities. Research on the coupling effects between the rates of nitrogen fertilizer and the soil types on soil microbial metabolic activities under field conditions are currently lacking. In this study, we investigated the effect of five different N fertilization rates on three types of soils (meadow black, alluvial, and sandy soil) that were over 8-year-long planted maize. Illumina Miseq sequencing methods were used to characterize the soil microbial communities. Real-time PCR were adopted to calculate the relative abundance of N, C, and P-cycling functional microbial. Results indicated that different N rates can significantly change soil available nutrients and effectively reduce soil pH. The structure of the soil bacterial communities and diversity did not change after the fertilization of N during the study period. However, it can be found that soil types can substantially influence the structure of bacterial communities in soil, meanwhile, there was a significant correlation between pH and the composition of bacterial communities. Although the composition of the soil microbial community did not influence the fertilization rate of N, they can be indirectly affected by pH. There was significant difference (P<0.05) in the relative abundances of the nutrient cycling functional genes of the rhizosphere soil under different nitrogen levels, with nitrogen application and soil type the main influencing factors. The results showed that the excessive application of N fertilizer did not increase the yields of maize, thus it is important to investigate the reasonable fertilization rates of N fertilizer to protect the ecological balance of crop soils.
Nuclear-encoded chloroplast small heat shock proteins (Cp-sHSPs) play important roles in plant stress tolerance due to their abundance and diversity. Their functions in Primula under heat treatment are poorly characterized. Here, expression analysis showed that the Primula Cp-sHSP gene, PfHSP21.4, was highly induced by heat stress in all vegetative and generative tissues in addition to constitutive expression in certain development stages. PfHSP21.4 was introduced into Arabidopsis, and its function was analysed in transgenic plants. Under heat stress, the PfHSP21.4 transgenic plants showed increased heat tolerance as shown by preservation of hypocotyl elongation, membrane integrity, chlorophyll content and photosystem II activity (Fv/Fm), increased seedling survival and increase in proline content. Alleviation of oxidative damage was associated with increased activity of superoxide dismutase and peroxidase. In addition, the induced expression of HSP101, HSP70, ascorbate peroxidase and Δ1-pyrroline-5-carboxylate synthase under heat stress was more pronounced in transgenic plants than in wild-type plants. These results support the positive role of PfHSP21.4 in response to heat stress in plants.
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