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1

Inland free-living nematodes in China: a historical review of taxonomic studies with descriptions of Aphanonchus orientalis sp.nov. [Chromadorida: Leptolaimidae] and Daptonema limnobia sp.nov. [Monchysterida: Xyalidae]

100%
Wu J., Liang Y.
Annales Zoologici
|
2000
|
tom 50
|
nr 2
The study of inland free-living nematodes is relatively imperfect in China, only seventeen papers were previously published. Since the early researches in 20-30s, few works have been accomplished until 80s. Altogether 171 taxa were formerly recorded, among which, over eighty species have been re-combined. A checklist of the former records with notes on their distribution is presented in this paper. Recently, the function of free-living nematodes has received much attention from Chinese zoologists. Hence, the present authors carried out their studies with emphasis on taxonomy of inland nematodes. During the survey of freshwater lakes, two species are found to be new to science. Aphanonchus orientalis sp. nov. is characterized by having sclerotized vagina, the presence of 10-11 tubular supplements and 42-62 alveoli supplements in males, but no alveoli in females. Daptonema limnobia sp. nov. is distinguished from other species of the genus in the presence of larger and more anteriorly located amphids, shorter bifurcated spicules, smaller apophysis of gubernaculum, shorter terminal setae, and postvulval uterine sac in females.
2

Effects of niclosamide on Oncomelania hupensis, the intermediate snail host of Schistosoma japonicum: an enzyme-histochemical study

81%
Wang W., Li H., Liang Y., Dai J.
Acta Parasitologica
|
2009
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tom 54
|
nr 2
172-179
An enzyme-histochemical study of five enzymes, namely succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), cytochrome oxidase (CCO), cholinesterase (CHE) and nitric oxide synthase (NOS), was elucidated in the soft tissues of Oncomelania hupensis, the intermediate host snail of Schistosoma japonicum, before and after the treatment with a suspension concentrate of niclosamide (SCN). Following the treatment of SCN, a marked loss occurred in the activity of the five enzymes mentioned above. LDH and SDH showed their strongest activity in the buccal mass and muscular fibers, CCO in buccal mass and liver, CHE in pellicle and ganglia, and NOS in muscular fibers and pharyngeal canal. The results indicate that SCN impairs the activities of the enzymes influencing the transfer of neurotransmitter and energy supply in O. hupensis, ultimately leading to the loss of various physiological functions, which is considered to be a cause of death in O. hupensis.
3

Histochemical and enzyme-histochemical studies of Oncomelania hupensis (Gredler, 1881), the intermediate host of Schistosoma japonicum

81%
Wang W., Dai J., Li H., Liang Y.
Acta Parasitologica
|
2011
|
tom 56
|
nr 1
The distribution of glycogen, DNA and histone, and localization of activity of ten enzymes such as glucose-6-phosphatase (G-6-Pase), cytochrome oxidase (CCO), lactate dehydrogenase (LDH), 5′-nucleotidase (5′-NT), succinate dehydrogenase (SDH), glucose-6-phosphate dehydrogenase (G-6-PDH), alkaline phosphatase (ALP), acid phosphatase (ACP), Mg2+-adenosine triphosphatase (Mg2+-ATPase), and cholinesterase (CHE) in Oncomelania hupensis (Gredler, 1881) snails, the intermediate host of Schistosoma japonicum, was surveyed using the histochemical and enzyme-histochemical techniques. The results showed that the glycogen with high activity was widely distributed in snails. DNA showed its strongest reaction in testis. The histone was distributed in sites of the radula, parenchyma of penis and ovary. LDH and SDH were widely distributed in snails and all were abundant. Sites of the strongest enzyme activities of G-6-PDH were evident in the reproductive system together with central ganglia. Only the ovary showed a strong enzyme activity of G-6-Pase. Most tissues and organs contained CCO, and the activity of this enzyme was very strong. 5′-NT showed a strong enzyme activity in the ovary and testis. Mg2+-ATPase was localized in sites of the liver, stomach and reproductive gland. A strong enzyme activity of ALP appeared in the digestive system. ACP showed a low activity in snails. The central ganglia, nervous stem, liver, branchial duct, epithelia of the head and foot regions showed a very strong enzyme activity of CHE. The findings could provide a theoretical basis for development of highly effective molluscicides with low toxicity to other biota, as well as means for novel snail control strategies.
4

Enchytraeus chaoyangensis, a new terrestrial culture species [Enchytraeidae, Oligochaeta] from Northeastern China

81%
Xie Z., Liang Y., Wang H.
Acta Hydrobiologica
|
2000
|
tom 42
|
nr 1-2
5

Nitrogen-fixing bacterial communities exhibit strong spatial structure along a slope gradient in karst shrublands ecosystem

71%
Liang Y., He X., Chen X., Hu Y., Su Y.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 5
Environmental heterogeneity is a primary factor in determining soil microbial spatial patterns. However, few studies have demonstrated a link between environmental heterogeneity and the spatial patterns in free-living nitrogen-fixing bacteria, which are important in nitrogen cycling. Here, quantitative polymerase chain reaction and terminal restriction fragment length polymorphism were used to determine the spatial distribution of free-living nitrogen-fixing bacteria along a slope gradient. Plant communities were evaluated through field surveys, and basic soil properties were measured in the laboratory. Soil properties (e.g., soil available phosphorus, soil organic carbon, and total nitrogen) were higher in upper- and middle -slope soils than in lower-slope soils, while bacterial abundance was the opposite. Bacterial and plant communities all varied along the slope gradient. Redundancy analysis revealed that bacterial community composition was closely linked to pH, soil organic carbon, available phosphorus, and total nitrogen. These results indicate that free-living nitrogen-fixing bacterial communities show strong spatial structure along a slope gradient, and emphasize the importance of soil heterogeneity in affecting bacterial spatial patterns in a karst region.
6

Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa

71%
Qin L., Liang Y., Yang D., Xia G., Liu S.
Journal of Applied Genetics
|
2015
|
tom 56
|
nr 1
7

Ectopic overexpression of maize heat shock transcription factor gene ZmHsf04 confers increased thermo and salt-stress tolerance in transgenic Arabidopsis

71%
Jiang Y., Zheng Q., Chen L., Liang Y., Wu J.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 01
In our previous study, 25 Hsf gene homologues were identified and analyzed in maize. However, the exact functions of these gene family members are unclear at present. Here, we report the isolation and characterization of a strongly heat-induced HsfA2 gene from maize, ZmHsf04, and show its function in the heat/salt-stress response. Subcellular location assays showed that the ZmHsf04 protein is localized to the nucleus. Transcription activation data demonstrate that an AHA2 domain protein is necessary for the transcriptional activity of ZmHsf04. Furthermore, the function of ZmHsf04 was characterized in detail in transgenic plants of Arabidopsis thaliana Col-0. Overexpression of ZmHsf04 in Arabidopsis enhanced thermotolerance and positively regulated short-term-acquired thermotolerance. In addition, ZmHsf04 significantly improved salt tolerance and increased the sensitivity to abscisic acid in transgenic Arabidopsis. Overexpression of ZmHsf04 in Col-0 plants significantly up-regulated the expression of heat-specific HSP genes (AtHsp25.3-P, AtHsp18.2-CI, and AtHsp70B) and stress-related genes (AtAPX2 and AtGolS1) compared to the wild type. In summary, we have characterized a transcriptional regulator, ZmHsf04, which has important functions in enhancing heat and salt tolerance in plants.
8

S100A4 promotes invasion and angiogenesis in breast cancer MDA-MB-231 cells by upregulating matrix metalloproteinase-13

71%
Wang L., Wang X., Liang Y., Diao X., Chen Q.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 4
S100A4 is a member of the S100 family of calcium-binding proteins that is directly involved in tumor metastasis. In the present study, we examined the potential role of S100A4 in metastasis in breast cancer and its relation with matrix metalloproteinase-13 (MMP-13). Analysis of 100 breast cancer specimens including 50 with and 50 without lymph node metastasis showed a significant upregulation of S100A4 and MMP-13 expression in metastatic breast cancer tissues. Positive immunoreactivity for S100A4 was associated with MMP-13 expression. Overexpression of S100A4 in the MDA-MB-231 breast cancer cell line upregulated MMP13 expression leading to increased cell migration and angiogenesis. SiRNA-mediated silencing of S100A4 downregulated MMP13 expression and suppressed cell migration and angiogenesis. Moreover, neutralization of MMP-13 activity with a specific antibody blocked cell migration and angiogenesis in MDA-MB-231/S100A4 cells. In vivo siRNA silencing of S100A4 significantly inhibited lung metastasis in transgenic mice. The present results suggest that the S100A4 gene may control the invasive potential of human breast cancer cells by modulating MMP-13 levels, thus regulating metastasis and angiogenesis in breast tumors. S100A4 could therefore be of value as a biomarker of breast cancer progression and a novel therapeutic target for human breast cancer treatment.
9

Enhancing nitrogen removal performance in a bioreactor using immobilized anaerobic ammonium oxidation sludge by polyvinyl alcohol-sodium alginate (PVA-SA)

71%
Lu Y., Ma L., Liang Y., Shan B., Chang J.
Polish Journal of Environmental Studies
|
2018
|
tom 27
|
nr 2
Anaerobic ammonium oxidation (anammox) is an efficient and promising pathway for nitrogen removal from wastewater, but its application is usually confined by the low growth rate and susceptibility to surroundings of anammox bacteria. In this study, cultured anammox sludge was immobilized using polyvinyl alcohol (PVA)-sodium alginate (SA) gel and put into a lab-scale column reactor at a packing ratio of 20%, and nitrogen removal performance was evaluated at two hydraulic retention times (HRTs). Anammox was rapidly initiated in the reactor, with ammonium and nitrite removal efficiency reaching 82.3% and 84.7% after an operation period of 10 d. Nitrogen removal efficiency declined greatly after the reduction of HRT from 24 h to 12 h, but then recovered quickly, with an average TN removal rate of 84.5% and 0.43 kg·m⁻³·d⁻¹ achieved under the steady operational state. The immobilized anammox reactor performed significantly better and was more stable in nitrogen removal than that with anammox sludge inoculated directly, indicating the superiority of cell entrapment of anammox biomass in addition to its easy reservation. Nitrogen removal in the reactor increased after stable operation with the HRT. It has potential to apply immobilized anammox sludge entrapped by PVA-SA gel for the convenient establishment of an anammox reactor with stable and high nitrogen removal rates.
10

Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury

71%
Liang Y., Yang N., Pan G., Jin B., Ji W.
Cellular and Molecular Biology Letters
|
2018
|
tom 23
Background: Pulmonary inflammation and endothelial barrier permeability increase in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) induced by pro-inflammatory cytokines and matrix metalloproteinases (MMPs). However, the relationship between pro-inflammatory cytokines and MMPs in ALI/ARDS remains poorly understood. Methods: A lipopolysaccharide (LPS)-induced ALI rat model was established through intratracheal instillation. The wet/dry ratios of lung tissues were measured, and bronchoalveolar lavage fluid (BALF) was collected to test protein concentrations, total cell/macrophage numbers, and pro-inflammatory cytokine levels. LPS-treated alveolar macrophages were utilized in in vitro experiments. The expression and secretion of MMPs were respectively detected using quantitative PCR, Western blotting and ELISA assays. Results: The levels of IL-33 and MMP2/9 in BALF increased in all the ALI rats with severe lung injury. LPS-induced IL-33 autocrine upregulated the expression of MMP2 and MMP9 through activating STAT3. Neutralizing IL-33 in culture medium with specific antibodies suppressed the expression and secretion of MMP2 and MMP9 in LPS-treated alveolar macrophages. Consistently, eliminating IL-33 decreased the levels of MMP2 and MMP9 in BALF and alleviated lung injury in ALI rats. Conclusion: The IL-33/STAT3/MMP2/9 regulatory pathway is activated in alveolar macrophages during acute lung injury, which may exacerbate the pulmonary inflammation.
11

Influence of medium salt strength and nitrogen source on biomass and metabolite accumulation in adventitious root cultures of Pseudostellaria heterophylla

61%
Yin S., Liang Y., Gao W., Wang J., Jing S., Zhang Y., Liu H.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 08
We investigated the effects of medium salt strength and ammonia/nitrate ratio on biomass production and metabolites accumulation of adventitious root. The medium with full-strength Murashige and Skoog (MS) reached the highest growth rate (16.77), and the contents of saponin and polysaccharide reached the peak (i.e., 0.65 and 24.85 %) at 3/4 MS and 1 MS, respectively. In case of ammonia/nitrate ratio, a NH4+/NO3- ratio of 20:40 was optimal for the production of biomass and polysaccharide (23.27 %). In contrast, the content of saponin achieved the optimum (0.74 %) at a NH4+/NO3 - ratio of 30:30. In 5-L balloon-type bubble bioreactor (BTBB) cultures, an approximately 23-fold increase in biomass was recorded. The fresh weight (FW) and dry weight (DW) were 72.78 and 6.79 g per bioreactor with the contents of saponin (0.62 %) and polysaccharide (17.32 %), respectively. It indicated potential application to produce adventitious roots of pseudostellaria heterophylla with bioreactors on large scale in commercial.
12

The diversity, growth promoting abilities and anti-microbial activities of bacteria isolated from the fruiting body of Agaricus bisporus

61%
Xiang Q., Luo L., Liang Y., Chen Q., Zhang X., Gu Y.
Polish Journal of Microbiology
|
2017
|
tom 66
|
nr 2
Agaricus bisporus plays an important role in ecological processes and is one of the most widely cultivated mushrooms worldwide. Mushroom growth-promoting bacteria have been isolated from casing soil and compost, but microorganisms in the fruiting body have received only a little attention. To get an overview of phylogenetic diversity of microorganisms in the fruiting body of A. bisporus, as well as to screen antimicrobial and mushroom growth-promoting strains, and eventually intensify mushroom production, we isolated and characterized microorganisms from the fruiting body of A. bisporus. In total, 55 bacterial strains were isolated, among which nine isolates represented Actinomycetes. All the isolates were analyzed by 16S rRNA gene RFLP and sixteen representative strains by 16S rRNA gene sequencing. According to the phylogenetic analysis, eleven isolates represented the Gram-positive Bacillus, Lysinibacillus, Paenibacillus, Pandorea and Streptomyces genera, and five isolates belonged to the Gram-negative Alcaligenes and Pseudomonas genera. The bacteria isolated from the fruiting body of A. bisporus had broad-spectrum antimicrobial activities and potential mushroom growth-promoting abilities.
13

Influence of sucrose concentration and phosphate source on biomass and metabolite accumulation in adventitious roots of Pseudostellaria heterophylla

61%
Yin S., Gao W., Liang Y., Wang J., Liu H., Wei C., Zuo B.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 05
In this study, we investigated the induction of Pseudostellaria heterophylla adventitious root and the effects of sucrose concentration and phosphate source on biomass increase and metabolites accumulation. These roots were initially cultured in Murashige and Skoog medium for 4 weeks. IBA 3.0 mg L-1 proved to be the best auxin for inducing adventitious roots and the frequency of adventitious roots induced from roots (100 %) was higher than that from leaves (78 %) and stems (27 %). The medium with 4 % sucrose resulted in the optimum biomass i.e. 1.04 g/flask DW, and the content of saponin and polysaccharides reached the peak i.e. 0.676 and 24.4 %, respectively. With regards to phosphate source, 1.25 mM phosphate concentration was more favorable for biomass of roots (0.87 g/flask of DW), whereas the optimum saponin (0.74 %) and polysaccharides (22.09 %) were achieved with 2.5 mM phosphate. However, the saponin content at 2.5 mM phosphate did not show significant difference from the saponin content at 0.625 mM (0.69 %) or 3.75 mM phosphate (0.69 %).
14

Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes

61%
Wang D., Wang H., Qi Y., Liang Y., Zhang J., Yu L.
Folia Histochemica et Cytobiologica
|
2012
|
tom 50
|
nr 1
15

Assessment of the socio-economic impact of a water diversion project for a water-receiving area

61%
Peng Z., Yin J., Zhang L., Zhao J., Liang Y., Wang H.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 2
16

Role of CX3CL1 in diseases

61%
Liu W., Jiang L., Bian C., Liang Y., Xing R., Yishakea M., Dong J.
Archivum Immunologiae et Therapiae Experimentalis
|
2016
|
tom 64
|
nr 5
17

Physiological characterization of ‘stay green’ wheat cultivars during the grain filling stage under field growing conditions

52%
Chen J., Liang Y., Hu X., Wang X., Tan F., Zhang H., Ren Z., Luo P.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 5
Rye (Secale cereale L.) chromosome arm 1RS could delay leaf senescence, and change in H₂O₂ content is a useful index for weighing the ability to delay the senescence. Two wheat cultivars, Chuannong12 (CN12) and Chuannong 18 (CN18), harboring the wheat–rye 1BL/1RS translocated chromosome were investigated for H₂O₂ change and physiological index after flowering under field conditions, and MY11, the agronomical parent of both CN12 and CN18, was used as the control. A combined change in the peak value of CdSe/ZnS quantum dot (QD) fluorescence and morphological observation indicated that the H₂O₂ contents in CN12 and CN18 were generally lower than that in MY11. They both had higher values for net photosynthetic rate (Pn), stomatal conductance (Gs), Fv/F'm F'v/F'm, and photochemical quenching of PSII (qP) than MY11 only in the late measurement stage. Some small differences were also observed, such as CN12 and CN18 wheat cultivars having higher and longer photosynthetic competence than MY11 during the grain filling stage, which perhaps resulted from a mechanism for removing oxidative species, especially H₂O₂.
18

Comparison of the anesthesia effects of ketamine, dexmedetomidine and tiletamine-zolazepam with or without tramadol in cats

52%
Liang Y., Wang Z., Li L., Li J., Ma X., Chen S., Yin Y., Lu D.
Medycyna Weterynaryjna
|
2021
|
tom 77
|
nr 08
19

Stabilization/solidification treatment of cadmium-bearing-residue with magnesium slag

52%
Chen Y., Han F., Liang Y., Peng N., Peng B., Liu H., Wang Z., Zhao Z.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 1
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