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Effect of D-mannose treatment on different antioxidants, phenolics, protease activity, lipid peroxidation, DNA damage and cell death was investigated in coleoptiles of etiolated wheat seedlings. Modulations in these biochemical parameters were monitored up to 96 h after treatment at 24 h intervals. With accelerating effect on initial signs of cell death, i.e., appearance of long DNA fragmentation and no effect on initiation of terminal stage, i.e., internucleosomal nDNA fragmentation, mannose treatment (1 % = 56 mM) diminished the antioxidant activities in wheat coleoptiles. Mannose treatment decreased the catalase activity at all intervals, while APX and POD activities decreased at 72 h. Peroxidation of lipids increased at 72 h after mannose treatment. Levels of most of antioxidants, i.e., SOD, peroxidases and phenolics were raised during initial time period (24–48 h) of mannose treatment probably as an attempt to counter the stress effect. Protease activity gradually increased and protein content decreased with time in both treated and non-treated coleoptiles. Sharp decrease in CAT, APX and peroxidase activities and increase in lipid peroxidation at 72 h overlaps with apoptotic internucleosomal nDNA fragmentation in this organ. This coincidence points towards the importance of compromised antioxidant defense and involvement of reactive oxygen species in initiation of terminal stage of programmed cell death in wheat coleoptile. In conclusion, accelerating effect on DNA fragmentation and lipid peroxidation along with diminished antioxidant activities at the time of internucleosomal nDNA fragmentation, provide evidence for pro-apoptotic effect of D-mannose in wheat coleoptile.
Rising environmental concerns toward the use of synthetic chemicals as well as global acceptance of natural products has emphasized the need for exploring novel and sustainable sources of eco-friendly dyes for the textile sector. Our study was conducted to explore the natural colorant potential of black carrot (Daucus carota L.) plant residue for textile processing and optimizing conditions for cotton dyeing. Cotton fabric was dyed using black carrot-based dye, extracted in varying media. The results revealed that black carrot colorants produced darker shades when extracted in acidified methanol media. Excellent color strength of cotton fabrics were found at 70°C by coloring for 55 minutes using 4.0 g of salt (NaCl) as an exhausting agent. The bio mordants as well as chemical mordant were employed to improve color strength properties. In the case of chemical mordanting, 6% tannic acid and 8% iron sulphate as pre-mordant and 8% tannic acid and 2% iron sulphate as post-mordant showed maximum color strength values. Similarly, using bio mordanting, 2% of turmeric (Curcuma longa L.) and 6% of henna (Lawsonia inermis L.) extracts as pre-mordant, as well as 8% of turmeric and 4% of henna extracts as post-mordant showed maximum color strength values. Dyed cotton fabrics exhibited best color fastness properties in term of light, washing, dry and wet rubbing fastness.
Mustard (Brassica juncea L.) cultivars Alankar (salt-tolerant) and PBM16 (salt-sensitive) plants were grown with 50 mM NaCl and were sprayed with 0.1, 0.5, and 1.0 mM salicylic acid (SA) to study the physiological processes determining salt tolerance and to observe the influence of SA application on the alleviation of NaClinduced adverse effects. The content of leaf Na⁺, Cl⁻, H₂O₂, TBARS, and electrolyte leakage and the activity of SOD were higher in PBM16 than Alankar. In contrast, nutrients content, activity of APX and GR, glutathione content, photosynthetic and growth characteristics were higher in Alankar. Treatment of 50 mM NaCl resulted in increase of Na⁺ and Cl⁻, oxidative stress, activity of antioxidant enzymes and glutathione content, while nutrients content, photosynthetic, and growth characteristics decreased in both the cultivars. Application of 0.5 mM SA alleviated the negative effects of 50 mM NaCl maximally, but 1.0 mM SA proved inhibitory. The effect of SA was more conspicuous in Alankar than PBM16. It is concluded that the higher tolerance of Alankar was due to its lower leaf Na⁺ and Cl⁻ content, higher nutrients content, and efficient antioxidant metabolism. The application of 0.5 mM SA substantially alleviated salt-induced adverse effects in Alankar.
Air pollution in Pakistan is causing damage to health, environment and quality of life. Air pollution in Pakistan is not effectively monitored due to heavy cost involved in setting up ground stations. However, Satellite remote sensing can effectively monitor the air pollution in terms of Aerosol Optical Depth (AOD) at regional as well as global level. However, algorithms used to derive AOD from different sensors have some inherited differences which can pose challenges in monitoring regional AOD at high temporal resolution using more than one sensor. Therefore, this study focuses on comparison of four major satellite based AOD products namely Moderate Resolution Imaging SpectroRadiometer (MODIS), Multi-angle Imaging SpectroRadiometer (MISR), Ozone Monitoring Instrument multiwavelength (OMI) aerosol product and Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) with the ground based AErosol RObotic NETwork (AERONET) AOD which is only available from Lahore and Karachi in Pakistan. The correlation of various AOD products with AERONET AOD is estimated statistically through coefficient of determination (R²), Root Mean Square Error (RMSE), slope and intercept. It is noticed that MODIS is relatively accurate and reliable for monitoring air quality on operational bases over the land cover area of Lahore (R² = 0.78; RMSE = 0.18), whereas MISR over the coastal areas of Karachi (R² = 0.82; RMSE = 0.20). The results of the study will help the stakeholders in planning additional ground stations for operational monitoring of air quality at regional level.
Didelphimorph marsupials were widely distributed in Asia during the Cenozoic, but their occurrence in the Indian subcontinent has not so far been demonstrated. Here, we describe a new herpetotheriid marsupial Asiadidelphis akbarbugtiisp. nov. from the early Oligocene Bugti Member of the Chitarwata Formation, Bugti Hills, Pakistan. The discovery of the herpetotheriids in the Oligocene of Pakistan represents the most southern occurrence of the family, which was thought to have occurred only to the north of the Alpine−Himalayan Suture. Our data suggest that episodic faunal exchanges occurred between the Asian mainland and the Indian subcontinent during the late Paleogene, and that the southern Asian faunas were not as completely isolated by the Himalayan chain as formerly believed.
Continuous rainfall during the harvest period of soybean leads to the delayed harvest of mature seeds and favors the infection of field mold that eventually causes seed mildew in the field under high humid conditions. Soybean isoflavones are a subgroup of flavonoids which possess crucial roles in the responses of soybean to multiple abiotic and/or biotic stresses. To fully reveal the role of isoflavones in the responses of soybean seeds to filed mildew (FM) stress, two soybean genotypes with different resistance to FM stress were treated in a controlled greenhouse which provided high humid conditions in this current research. The dynamic changes of isoflavones contents and the expression levels of four major genes involved in isoflavones biosynthesis pathway were measured by liquid chromatography–mass spectrometry (LC–MS) and quantitative real-time PCR (qRT-PCR), respectively. The current results showed that the contents of major isoflavones components in resistant genotype C103 were higher than the susceptive genotype ND12 before FM treatment. Whereas after FM treatment, the dynamic changing patterns of isoflavones contents and some major genes expression were opposite between these two genotypes, and the biosynthesis of isoflavones was more rapid in resistant genotype compared to susceptible one. Noticeably, the biosynthesis of isoflavones aglycones which exhibit better bioactivities was remarkably promoted after FM treatment in resistant genotype C103, further revealing the important role of isoflavones in the resistance against FM stress. In sum, this research investigated the role of isoflavones against FM stress, the results demonstrated higher inherent contents and more rapid biosynthesis of isoflavones after FM treatment could establish stronger resistant foundation in soybean seeds against the FM stress. These results would provide new insights into the mechanism research of soybean against FM stress, but how the stress systematically regulates isoflavones and other resistant network still needs further research.
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