The aim of presented research was an evaluation of fungistatic activity of two strains of Paenibacillus species including preliminary characteristics of metabolites responsible for antagonism. The tested strains inhibited growth of all fungi used as indicators however belonging to Microdochium and Septoria genera were more sensitive than Fusarium. It was observed that larger amount of Paenibacillus sp. added to culture caused usually stronger inhibition of fungal growth and antifungal effect was comparable for both strains. Enzymatic hydrolysis revealed completely lost of antagonistic activity, what indicates that fungistatic metabolites are proteins.